Cell block cytology : Improved preparation and its efficacy in diagnostic cytology
Cell blocks prepared from residual tissue fluids and fine-needle aspirations can be useful adjuncts to smears for establishing a more definitive cytopathologic diagnosis. They can be particularly useful for categorization of tumors that otherwise may not be possible from smears themselves. A modifie...
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| Veröffentlicht in: | American journal of clinical pathology 2000-10, Vol.114 (4), p.599-606 |
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| container_title | American journal of clinical pathology |
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| creator | NATHAN, Nithyananda A NARAYAN, Eddie SMITH, Mary M HORN, Murray J |
| description | Cell blocks prepared from residual tissue fluids and fine-needle aspirations can be useful adjuncts to smears for establishing a more definitive cytopathologic diagnosis. They can be particularly useful for categorization of tumors that otherwise may not be possible from smears themselves. A modified cell block technique using an improvised ethanol formalin fixative (Nathan alcohol formalin substitute) followed by a simple paraffin processing schedule is described. This improved preparation offers excellent cytomorphologic features corresponding closely to cells in Papanicolaou-stained smears and ensures optimal preservation of histochemical and immunocytochemical properties. The technique is simple and reproducible and uses routine safe laboratory chemicals. The efficacy of cell blocks also is discussed. |
| doi_str_mv | 10.1309/G035-P2MM-D1TM-T5QE |
| format | Article |
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The efficacy of cell blocks also is discussed.</description><subject>Biological and medical sciences</subject><subject>Biopsy, Needle - methods</subject><subject>Body Fluids - cytology</subject><subject>Cytodiagnosis - methods</subject><subject>Exudates and Transudates - cytology</subject><subject>Female</subject><subject>Humans</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Miscellaneous. Technology</subject><subject>Neoplasms - chemistry</subject><subject>Neoplasms - pathology</subject><subject>Paraffin Embedding</subject><subject>Pathology. Cytology. Biochemistry. Spectrometry. 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They can be particularly useful for categorization of tumors that otherwise may not be possible from smears themselves. A modified cell block technique using an improvised ethanol formalin fixative (Nathan alcohol formalin substitute) followed by a simple paraffin processing schedule is described. This improved preparation offers excellent cytomorphologic features corresponding closely to cells in Papanicolaou-stained smears and ensures optimal preservation of histochemical and immunocytochemical properties. The technique is simple and reproducible and uses routine safe laboratory chemicals. The efficacy of cell blocks also is discussed.</abstract><cop>Chicago, IL</cop><pub>American Society of Clinical Pathologists</pub><pmid>11026107</pmid><doi>10.1309/G035-P2MM-D1TM-T5QE</doi><tpages>8</tpages></addata></record> |
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| identifier | ISSN: 0002-9173 |
| ispartof | American journal of clinical pathology, 2000-10, Vol.114 (4), p.599-606 |
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| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals |
| subjects | Biological and medical sciences Biopsy, Needle - methods Body Fluids - cytology Cytodiagnosis - methods Exudates and Transudates - cytology Female Humans Investigative techniques, diagnostic techniques (general aspects) Male Medical sciences Miscellaneous. Technology Neoplasms - chemistry Neoplasms - pathology Paraffin Embedding Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Reproducibility of Results Sensitivity and Specificity Tissue Fixation - methods |
| title | Cell block cytology : Improved preparation and its efficacy in diagnostic cytology |
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