Role of insulin receptor substrate-2 in interleukin-9-dependent proliferation
Interleukin-9 (IL-9) stimulation results in JAK, STAT and IRS1/2 phosphorylation. The role of IRS adaptor proteins in IL-9 signaling is not clear. We show that IL-9 induces IRS2 phosphorylation and association with phosphatidylinositol-3 kinase (PI 3-K) p85 subunit in TS1 cells and BaF/9R cells, whi...
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| Veröffentlicht in: | FEBS letters 2000-10, Vol.482 (3), p.200-204 |
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| creator | Demoulin, Jean-Baptiste Grasso, Luigi Atkins, John M. Stevens, Monique Louahed, Jamila Levitt, Roy C. Nicolaides, Nicholas C. Renauld, Jean-Christophe |
| description | Interleukin-9 (IL-9) stimulation results in JAK, STAT and IRS1/2 phosphorylation. The role of IRS adaptor proteins in IL-9 signaling is not clear. We show that IL-9 induces IRS2 phosphorylation and association with phosphatidylinositol-3 kinase (PI 3-K) p85 subunit in TS1 cells and BaF/9R cells, which proliferate upon IL-9 stimulation. We observed a PI 3-K-dependent phosphorylation of protein kinase B (PKB) in TS1 cells, but not in BaF/9R, nor in other IL-9-dependent cell lines. Finally, 32D cells that were transfected with the IL-9 receptor but lack IRS expression survived in the presence of IL-9. Ectopic IRS1 expression allowed for IL-9-induced proliferation, in the absence of significant PKB phosphorylation. |
| doi_str_mv | 10.1016/S0014-5793(00)02059-7 |
| format | Article |
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The role of IRS adaptor proteins in IL-9 signaling is not clear. We show that IL-9 induces IRS2 phosphorylation and association with phosphatidylinositol-3 kinase (PI 3-K) p85 subunit in TS1 cells and BaF/9R cells, which proliferate upon IL-9 stimulation. We observed a PI 3-K-dependent phosphorylation of protein kinase B (PKB) in TS1 cells, but not in BaF/9R, nor in other IL-9-dependent cell lines. Finally, 32D cells that were transfected with the IL-9 receptor but lack IRS expression survived in the presence of IL-9. Ectopic IRS1 expression allowed for IL-9-induced proliferation, in the absence of significant PKB phosphorylation.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/S0014-5793(00)02059-7</identifier><identifier>PMID: 11024460</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Animals ; Apoptosis ; Cell Division - physiology ; Cells, Cultured ; Extracellular Matrix Proteins - metabolism ; Humans ; IL, interleukin ; IL-9R, interleukin-9 receptor ; Insulin receptor substrate ; Insulin Receptor Substrate Proteins ; Interleukin-9 ; Interleukin-9 - physiology ; Intracellular Signaling Peptides and Proteins ; IRS, insulin receptor substrate ; JAK, janus kinase ; Mice ; Phosphatidylinositol 3-Kinases - metabolism ; Phosphoproteins - biosynthesis ; Phosphoproteins - physiology ; Phosphorylation ; PI 3-K, phosphatidylinositol-3 kinase ; PKB, protein kinase B ; Proliferation ; Protein kinase B ; Protein-Serine-Threonine Kinases ; Proto-Oncogene Proteins - metabolism ; Proto-Oncogene Proteins c-akt ; STAT, signal transducer and activator transcription factor</subject><ispartof>FEBS letters, 2000-10, Vol.482 (3), p.200-204</ispartof><rights>2000 Federation of European Biochemical Societies</rights><rights>FEBS Letters 482 (2000) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4737-94bc7710034639445793f63d477813f91e5658f0c4260a9482849ba79fbdbe7d3</citedby><cites>FETCH-LOGICAL-c4737-94bc7710034639445793f63d477813f91e5658f0c4260a9482849ba79fbdbe7d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2FS0014-5793%2800%2902059-7$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0014-5793(00)02059-7$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>315,781,785,1418,1434,3551,27926,27927,45576,45577,45997,46411,46835</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11024460$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Demoulin, Jean-Baptiste</creatorcontrib><creatorcontrib>Grasso, Luigi</creatorcontrib><creatorcontrib>Atkins, John M.</creatorcontrib><creatorcontrib>Stevens, Monique</creatorcontrib><creatorcontrib>Louahed, Jamila</creatorcontrib><creatorcontrib>Levitt, Roy C.</creatorcontrib><creatorcontrib>Nicolaides, Nicholas C.</creatorcontrib><creatorcontrib>Renauld, Jean-Christophe</creatorcontrib><title>Role of insulin receptor substrate-2 in interleukin-9-dependent proliferation</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>Interleukin-9 (IL-9) stimulation results in JAK, STAT and IRS1/2 phosphorylation. The role of IRS adaptor proteins in IL-9 signaling is not clear. We show that IL-9 induces IRS2 phosphorylation and association with phosphatidylinositol-3 kinase (PI 3-K) p85 subunit in TS1 cells and BaF/9R cells, which proliferate upon IL-9 stimulation. We observed a PI 3-K-dependent phosphorylation of protein kinase B (PKB) in TS1 cells, but not in BaF/9R, nor in other IL-9-dependent cell lines. Finally, 32D cells that were transfected with the IL-9 receptor but lack IRS expression survived in the presence of IL-9. Ectopic IRS1 expression allowed for IL-9-induced proliferation, in the absence of significant PKB phosphorylation.</description><subject>Animals</subject><subject>Apoptosis</subject><subject>Cell Division - physiology</subject><subject>Cells, Cultured</subject><subject>Extracellular Matrix Proteins - metabolism</subject><subject>Humans</subject><subject>IL, interleukin</subject><subject>IL-9R, interleukin-9 receptor</subject><subject>Insulin receptor substrate</subject><subject>Insulin Receptor Substrate Proteins</subject><subject>Interleukin-9</subject><subject>Interleukin-9 - physiology</subject><subject>Intracellular Signaling Peptides and Proteins</subject><subject>IRS, insulin receptor substrate</subject><subject>JAK, janus kinase</subject><subject>Mice</subject><subject>Phosphatidylinositol 3-Kinases - metabolism</subject><subject>Phosphoproteins - biosynthesis</subject><subject>Phosphoproteins - physiology</subject><subject>Phosphorylation</subject><subject>PI 3-K, phosphatidylinositol-3 kinase</subject><subject>PKB, protein kinase B</subject><subject>Proliferation</subject><subject>Protein kinase B</subject><subject>Protein-Serine-Threonine Kinases</subject><subject>Proto-Oncogene Proteins - metabolism</subject><subject>Proto-Oncogene Proteins c-akt</subject><subject>STAT, signal transducer and activator transcription factor</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNUV1LwzAUDaK4-fETlD6JPkRvmrRpnkTH5oSJ4Mdz6MctRLt2Jq2yf2-6Dn1UCITknHvO4VxCThhcMmDx1TMAEzSSip8DXEAIkaJyh4xZIjnlIk52yfiHMiIHzr2BfydM7ZMRYxAKEcOYPDw1FQZNGZjadZWpA4s5rtrGBq7LXGvTFmnoQX9atBV276amiha4wrrAug1WtqlMiZ5omvqI7JVp5fB4ex-S19n0ZTKni8e7-8nNguZCckmVyHIpGYDPyZUQfcQy5oWQMmG8VAyjOEpKyEUYQ6pEEiZCZalUZVZkKAt-SM4GXe_-0aFr9dK4HKsqrbHpnJYhZ5LFiSdGAzG3jXMWS72yZpnatWag-x71pkfdJ9AAetOjln7udGvQZUssfqe2xXnCfCB8mQrX_1PVs-ltuEF6wG-j_-69rgcp9I19GrTa5QbrHAvjl9HqojF_pP0G0fOU3Q</recordid><startdate>20001006</startdate><enddate>20001006</enddate><creator>Demoulin, Jean-Baptiste</creator><creator>Grasso, Luigi</creator><creator>Atkins, John M.</creator><creator>Stevens, Monique</creator><creator>Louahed, Jamila</creator><creator>Levitt, Roy C.</creator><creator>Nicolaides, Nicholas C.</creator><creator>Renauld, Jean-Christophe</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20001006</creationdate><title>Role of insulin receptor substrate-2 in interleukin-9-dependent proliferation</title><author>Demoulin, Jean-Baptiste ; Grasso, Luigi ; Atkins, John M. ; Stevens, Monique ; Louahed, Jamila ; Levitt, Roy C. ; Nicolaides, Nicholas C. ; Renauld, Jean-Christophe</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4737-94bc7710034639445793f63d477813f91e5658f0c4260a9482849ba79fbdbe7d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>Apoptosis</topic><topic>Cell Division - physiology</topic><topic>Cells, Cultured</topic><topic>Extracellular Matrix Proteins - metabolism</topic><topic>Humans</topic><topic>IL, interleukin</topic><topic>IL-9R, interleukin-9 receptor</topic><topic>Insulin receptor substrate</topic><topic>Insulin Receptor Substrate Proteins</topic><topic>Interleukin-9</topic><topic>Interleukin-9 - physiology</topic><topic>Intracellular Signaling Peptides and Proteins</topic><topic>IRS, insulin receptor substrate</topic><topic>JAK, janus kinase</topic><topic>Mice</topic><topic>Phosphatidylinositol 3-Kinases - metabolism</topic><topic>Phosphoproteins - biosynthesis</topic><topic>Phosphoproteins - physiology</topic><topic>Phosphorylation</topic><topic>PI 3-K, phosphatidylinositol-3 kinase</topic><topic>PKB, protein kinase B</topic><topic>Proliferation</topic><topic>Protein kinase B</topic><topic>Protein-Serine-Threonine Kinases</topic><topic>Proto-Oncogene Proteins - metabolism</topic><topic>Proto-Oncogene Proteins c-akt</topic><topic>STAT, signal transducer and activator transcription factor</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Demoulin, Jean-Baptiste</creatorcontrib><creatorcontrib>Grasso, Luigi</creatorcontrib><creatorcontrib>Atkins, John M.</creatorcontrib><creatorcontrib>Stevens, Monique</creatorcontrib><creatorcontrib>Louahed, Jamila</creatorcontrib><creatorcontrib>Levitt, Roy C.</creatorcontrib><creatorcontrib>Nicolaides, Nicholas C.</creatorcontrib><creatorcontrib>Renauld, Jean-Christophe</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Demoulin, Jean-Baptiste</au><au>Grasso, Luigi</au><au>Atkins, John M.</au><au>Stevens, Monique</au><au>Louahed, Jamila</au><au>Levitt, Roy C.</au><au>Nicolaides, Nicholas C.</au><au>Renauld, Jean-Christophe</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Role of insulin receptor substrate-2 in interleukin-9-dependent proliferation</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>2000-10-06</date><risdate>2000</risdate><volume>482</volume><issue>3</issue><spage>200</spage><epage>204</epage><pages>200-204</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>Interleukin-9 (IL-9) stimulation results in JAK, STAT and IRS1/2 phosphorylation. The role of IRS adaptor proteins in IL-9 signaling is not clear. We show that IL-9 induces IRS2 phosphorylation and association with phosphatidylinositol-3 kinase (PI 3-K) p85 subunit in TS1 cells and BaF/9R cells, which proliferate upon IL-9 stimulation. We observed a PI 3-K-dependent phosphorylation of protein kinase B (PKB) in TS1 cells, but not in BaF/9R, nor in other IL-9-dependent cell lines. Finally, 32D cells that were transfected with the IL-9 receptor but lack IRS expression survived in the presence of IL-9. Ectopic IRS1 expression allowed for IL-9-induced proliferation, in the absence of significant PKB phosphorylation.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>11024460</pmid><doi>10.1016/S0014-5793(00)02059-7</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Access via Wiley Online Library; Access via ScienceDirect (Elsevier); EZB-FREE-00999 freely available EZB journals; Wiley Online Library (Open Access Collection); Alma/SFX Local Collection |
| subjects | Animals Apoptosis Cell Division - physiology Cells, Cultured Extracellular Matrix Proteins - metabolism Humans IL, interleukin IL-9R, interleukin-9 receptor Insulin receptor substrate Insulin Receptor Substrate Proteins Interleukin-9 Interleukin-9 - physiology Intracellular Signaling Peptides and Proteins IRS, insulin receptor substrate JAK, janus kinase Mice Phosphatidylinositol 3-Kinases - metabolism Phosphoproteins - biosynthesis Phosphoproteins - physiology Phosphorylation PI 3-K, phosphatidylinositol-3 kinase PKB, protein kinase B Proliferation Protein kinase B Protein-Serine-Threonine Kinases Proto-Oncogene Proteins - metabolism Proto-Oncogene Proteins c-akt STAT, signal transducer and activator transcription factor |
| title | Role of insulin receptor substrate-2 in interleukin-9-dependent proliferation |
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