First Isolation of Stephanoascus ciferrii from a Cat
The present study deals with the first isolation of Stephanoascus ciferrii from a cat. A 2-year-old female Persian cat weighing 2.25kg was referred to an animal hospital with a chief complaint of otitis externa of the left ear. Microscopic examination of specimen from the left ear disclosed yeast ce...
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Veröffentlicht in: | MICROBIOLOGY and IMMUNOLOGY 2000, Vol.44(8), pp.711-713 |
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creator | Kano, Rui Makimura, Koichi Kushida, Toshiaki Nomura, Megumi Yamaguchi, Hideo Hasegawa, Atsuhiko |
description | The present study deals with the first isolation of Stephanoascus ciferrii from a cat. A 2-year-old female Persian cat weighing 2.25kg was referred to an animal hospital with a chief complaint of otitis externa of the left ear. Microscopic examination of specimen from the left ear disclosed yeast cells. The colony of the clinical isolate was cream-colored, rough, raised and wrinkled. The microscopic examination of the clinical isolate revealed abundant branched and septated mycelia with small ramified chains of oval blastoconidia, variable in size, and arranged alongside the hyphae. Amplification of the isolate DNA with LSU rDNA primers yielded a fragment of about 570bp, whose nucleotide sequence of the isolate showed 100% similarity to that of Stephanoascus ciferrii in the GenBank database. Therefore, the isolate was identified as Stephanoascus ciferrii, confirming the result of mycological examination by molecular analysis. |
doi_str_mv | 10.1111/j.1348-0421.2000.tb02553.x |
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A 2-year-old female Persian cat weighing 2.25kg was referred to an animal hospital with a chief complaint of otitis externa of the left ear. Microscopic examination of specimen from the left ear disclosed yeast cells. The colony of the clinical isolate was cream-colored, rough, raised and wrinkled. The microscopic examination of the clinical isolate revealed abundant branched and septated mycelia with small ramified chains of oval blastoconidia, variable in size, and arranged alongside the hyphae. Amplification of the isolate DNA with LSU rDNA primers yielded a fragment of about 570bp, whose nucleotide sequence of the isolate showed 100% similarity to that of Stephanoascus ciferrii in the GenBank database. Therefore, the isolate was identified as Stephanoascus ciferrii, confirming the result of mycological examination by molecular analysis.</description><identifier>ISSN: 0385-5600</identifier><identifier>EISSN: 1348-0421</identifier><identifier>DOI: 10.1111/j.1348-0421.2000.tb02553.x</identifier><identifier>PMID: 11021402</identifier><identifier>CODEN: MIIMDV</identifier><language>eng</language><publisher>Tokyo: Blackwell Publishing Ltd</publisher><subject>Animals ; Biological and medical sciences ; Cat ; Cat Diseases - microbiology ; Cats ; DNA, Ribosomal ; Experimental mycoses and models ; Female ; Genes, Fungal ; Infectious diseases ; Medical sciences ; Mycoses ; Mycoses - microbiology ; Mycoses - veterinary ; Otitis externa ; Otitis Externa - microbiology ; Otitis Externa - veterinary ; Ribosomal DNA sequence ; Saccharomycetales - genetics ; Saccharomycetales - isolation & purification ; Stephanoascus ciferrii</subject><ispartof>MICROBIOLOGY and IMMUNOLOGY, 2000, Vol.44(8), pp.711-713</ispartof><rights>Center for Academic Publications Japan</rights><rights>owned by Center for Academic Publications Japan (Publisher)</rights><rights>2000 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c7483-256964c27f50a2eb2d8d77a47caec08c8d0b78554e5e8d95cd7a8fbb3a83111b3</citedby><cites>FETCH-LOGICAL-c7483-256964c27f50a2eb2d8d77a47caec08c8d0b78554e5e8d95cd7a8fbb3a83111b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1348-0421.2000.tb02553.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1348-0421.2000.tb02553.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,777,781,1412,1428,1877,27905,27906,45555,45556,46390,46814</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1513690$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11021402$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kano, Rui</creatorcontrib><creatorcontrib>Makimura, Koichi</creatorcontrib><creatorcontrib>Kushida, Toshiaki</creatorcontrib><creatorcontrib>Nomura, Megumi</creatorcontrib><creatorcontrib>Yamaguchi, Hideo</creatorcontrib><creatorcontrib>Hasegawa, Atsuhiko</creatorcontrib><title>First Isolation of Stephanoascus ciferrii from a Cat</title><title>MICROBIOLOGY and IMMUNOLOGY</title><addtitle>Microbiology and Immunology</addtitle><description>The present study deals with the first isolation of Stephanoascus ciferrii from a cat. A 2-year-old female Persian cat weighing 2.25kg was referred to an animal hospital with a chief complaint of otitis externa of the left ear. Microscopic examination of specimen from the left ear disclosed yeast cells. The colony of the clinical isolate was cream-colored, rough, raised and wrinkled. The microscopic examination of the clinical isolate revealed abundant branched and septated mycelia with small ramified chains of oval blastoconidia, variable in size, and arranged alongside the hyphae. Amplification of the isolate DNA with LSU rDNA primers yielded a fragment of about 570bp, whose nucleotide sequence of the isolate showed 100% similarity to that of Stephanoascus ciferrii in the GenBank database. Therefore, the isolate was identified as Stephanoascus ciferrii, confirming the result of mycological examination by molecular analysis.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cat</subject><subject>Cat Diseases - microbiology</subject><subject>Cats</subject><subject>DNA, Ribosomal</subject><subject>Experimental mycoses and models</subject><subject>Female</subject><subject>Genes, Fungal</subject><subject>Infectious diseases</subject><subject>Medical sciences</subject><subject>Mycoses</subject><subject>Mycoses - microbiology</subject><subject>Mycoses - veterinary</subject><subject>Otitis externa</subject><subject>Otitis Externa - microbiology</subject><subject>Otitis Externa - veterinary</subject><subject>Ribosomal DNA sequence</subject><subject>Saccharomycetales - genetics</subject><subject>Saccharomycetales - isolation & purification</subject><subject>Stephanoascus ciferrii</subject><issn>0385-5600</issn><issn>1348-0421</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkF1v0zAUhi0EYmXwF1CEEHcJ_oxdrpiqfVRah4AhuDs6cRzmkibFTkX37-eSsHGH8IVt6Tzn8fFLyCtGC5bW23XBhDQ5lZwVnFJaDBXlSoli_4jM7kuPyYwKo3JVUnpEnsW4ppRrbuRTcsQY5UxSPiPyzIc4ZMvYtzj4vsv6Jvs8uO0Ndj1Gu4uZ9Y0LwfusCf0mw2yBw3PypME2uhfTeUy-nJ1eLy7yyw_ny8XJZW61NCLnqpyX0nLdKIrcVbw2tdYotUVnqbGmppU2SkmnnKnnytYaTVNVAo1I_6zEMXkzereh_7lzcYCNj9a1LXau30XQXDDFTPlPkOk0imIyge9G0IY-xuAa2Aa_wXALjMIhXFjDIUE4JAiHcGEKF_ap-eX0yq7auPqhdUozAa8nIEWHbROwsz4-cIqJck4T9n7EfvnW3f7HBLBarn5fk-J0VKzjgN_dvQPD4G3rYINd7dlca5ASzLjp5P5TtzcYwHXJk48eHwe3_0vzA0ottIKvV-fwqbz-dsX1R1iJO22TvM4</recordid><startdate>20000101</startdate><enddate>20000101</enddate><creator>Kano, Rui</creator><creator>Makimura, Koichi</creator><creator>Kushida, Toshiaki</creator><creator>Nomura, Megumi</creator><creator>Yamaguchi, Hideo</creator><creator>Hasegawa, Atsuhiko</creator><general>Blackwell Publishing Ltd</general><general>Center For Academic Publications Japan</general><general>Center for Academic Publications Japan</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>20000101</creationdate><title>First Isolation of Stephanoascus ciferrii from a Cat</title><author>Kano, Rui ; Makimura, Koichi ; Kushida, Toshiaki ; Nomura, Megumi ; Yamaguchi, Hideo ; Hasegawa, Atsuhiko</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c7483-256964c27f50a2eb2d8d77a47caec08c8d0b78554e5e8d95cd7a8fbb3a83111b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cat</topic><topic>Cat Diseases - microbiology</topic><topic>Cats</topic><topic>DNA, Ribosomal</topic><topic>Experimental mycoses and models</topic><topic>Female</topic><topic>Genes, Fungal</topic><topic>Infectious diseases</topic><topic>Medical sciences</topic><topic>Mycoses</topic><topic>Mycoses - microbiology</topic><topic>Mycoses - veterinary</topic><topic>Otitis externa</topic><topic>Otitis Externa - microbiology</topic><topic>Otitis Externa - veterinary</topic><topic>Ribosomal DNA sequence</topic><topic>Saccharomycetales - genetics</topic><topic>Saccharomycetales - isolation & purification</topic><topic>Stephanoascus ciferrii</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kano, Rui</creatorcontrib><creatorcontrib>Makimura, Koichi</creatorcontrib><creatorcontrib>Kushida, Toshiaki</creatorcontrib><creatorcontrib>Nomura, Megumi</creatorcontrib><creatorcontrib>Yamaguchi, Hideo</creatorcontrib><creatorcontrib>Hasegawa, Atsuhiko</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>MICROBIOLOGY and IMMUNOLOGY</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kano, Rui</au><au>Makimura, Koichi</au><au>Kushida, Toshiaki</au><au>Nomura, Megumi</au><au>Yamaguchi, Hideo</au><au>Hasegawa, Atsuhiko</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>First Isolation of Stephanoascus ciferrii from a Cat</atitle><jtitle>MICROBIOLOGY and IMMUNOLOGY</jtitle><addtitle>Microbiology and Immunology</addtitle><date>2000-01-01</date><risdate>2000</risdate><volume>44</volume><issue>8</issue><spage>711</spage><epage>713</epage><pages>711-713</pages><issn>0385-5600</issn><eissn>1348-0421</eissn><coden>MIIMDV</coden><abstract>The present study deals with the first isolation of Stephanoascus ciferrii from a cat. A 2-year-old female Persian cat weighing 2.25kg was referred to an animal hospital with a chief complaint of otitis externa of the left ear. Microscopic examination of specimen from the left ear disclosed yeast cells. The colony of the clinical isolate was cream-colored, rough, raised and wrinkled. The microscopic examination of the clinical isolate revealed abundant branched and septated mycelia with small ramified chains of oval blastoconidia, variable in size, and arranged alongside the hyphae. Amplification of the isolate DNA with LSU rDNA primers yielded a fragment of about 570bp, whose nucleotide sequence of the isolate showed 100% similarity to that of Stephanoascus ciferrii in the GenBank database. 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subjects | Animals Biological and medical sciences Cat Cat Diseases - microbiology Cats DNA, Ribosomal Experimental mycoses and models Female Genes, Fungal Infectious diseases Medical sciences Mycoses Mycoses - microbiology Mycoses - veterinary Otitis externa Otitis Externa - microbiology Otitis Externa - veterinary Ribosomal DNA sequence Saccharomycetales - genetics Saccharomycetales - isolation & purification Stephanoascus ciferrii |
title | First Isolation of Stephanoascus ciferrii from a Cat |
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