First Isolation of Stephanoascus ciferrii from a Cat

The present study deals with the first isolation of Stephanoascus ciferrii from a cat. A 2-year-old female Persian cat weighing 2.25kg was referred to an animal hospital with a chief complaint of otitis externa of the left ear. Microscopic examination of specimen from the left ear disclosed yeast ce...

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Veröffentlicht in:MICROBIOLOGY and IMMUNOLOGY 2000, Vol.44(8), pp.711-713
Hauptverfasser: Kano, Rui, Makimura, Koichi, Kushida, Toshiaki, Nomura, Megumi, Yamaguchi, Hideo, Hasegawa, Atsuhiko
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container_title MICROBIOLOGY and IMMUNOLOGY
container_volume 44
creator Kano, Rui
Makimura, Koichi
Kushida, Toshiaki
Nomura, Megumi
Yamaguchi, Hideo
Hasegawa, Atsuhiko
description The present study deals with the first isolation of Stephanoascus ciferrii from a cat. A 2-year-old female Persian cat weighing 2.25kg was referred to an animal hospital with a chief complaint of otitis externa of the left ear. Microscopic examination of specimen from the left ear disclosed yeast cells. The colony of the clinical isolate was cream-colored, rough, raised and wrinkled. The microscopic examination of the clinical isolate revealed abundant branched and septated mycelia with small ramified chains of oval blastoconidia, variable in size, and arranged alongside the hyphae. Amplification of the isolate DNA with LSU rDNA primers yielded a fragment of about 570bp, whose nucleotide sequence of the isolate showed 100% similarity to that of Stephanoascus ciferrii in the GenBank database. Therefore, the isolate was identified as Stephanoascus ciferrii, confirming the result of mycological examination by molecular analysis.
doi_str_mv 10.1111/j.1348-0421.2000.tb02553.x
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purification</topic><topic>Stephanoascus ciferrii</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kano, Rui</creatorcontrib><creatorcontrib>Makimura, Koichi</creatorcontrib><creatorcontrib>Kushida, Toshiaki</creatorcontrib><creatorcontrib>Nomura, Megumi</creatorcontrib><creatorcontrib>Yamaguchi, Hideo</creatorcontrib><creatorcontrib>Hasegawa, Atsuhiko</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>MICROBIOLOGY and IMMUNOLOGY</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kano, Rui</au><au>Makimura, Koichi</au><au>Kushida, Toshiaki</au><au>Nomura, Megumi</au><au>Yamaguchi, Hideo</au><au>Hasegawa, Atsuhiko</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>First Isolation of Stephanoascus ciferrii from a Cat</atitle><jtitle>MICROBIOLOGY and IMMUNOLOGY</jtitle><addtitle>Microbiology and Immunology</addtitle><date>2000-01-01</date><risdate>2000</risdate><volume>44</volume><issue>8</issue><spage>711</spage><epage>713</epage><pages>711-713</pages><issn>0385-5600</issn><eissn>1348-0421</eissn><coden>MIIMDV</coden><abstract>The present study deals with the first isolation of Stephanoascus ciferrii from a cat. 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subjects Animals
Biological and medical sciences
Cat
Cat Diseases - microbiology
Cats
DNA, Ribosomal
Experimental mycoses and models
Female
Genes, Fungal
Infectious diseases
Medical sciences
Mycoses
Mycoses - microbiology
Mycoses - veterinary
Otitis externa
Otitis Externa - microbiology
Otitis Externa - veterinary
Ribosomal DNA sequence
Saccharomycetales - genetics
Saccharomycetales - isolation & purification
Stephanoascus ciferrii
title First Isolation of Stephanoascus ciferrii from a Cat
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