Neuroendocrine differentiation of the LNCaP prostate cancer cell line maintains the expression and function of VIP and PACAP receptors
The molecular mechanisms involved in differentiation of prostate cancer cells to a neuroendocrine (NE) cell phenotype are not well understood. Here we used the androgen-dependent human prostate cancer cell line LNCaP to perform a systematic and broad analysis of the expression, pharmacology, and fun...
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| Veröffentlicht in: | Cellular signalling 2001-12, Vol.13 (12), p.887-894 |
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| creator | Juarranz, Marı́a G. Bolaños, Oscar Gutiérrez-Cañas, Irene Lerner, Ethan A. Robberecht, Patrick Carmena, Marı́a J. Prieto, Juan C. Rodrı́guez-Henche, Nieves |
| description | The molecular mechanisms involved in differentiation of prostate cancer cells to a neuroendocrine (NE) cell phenotype are not well understood. Here we used the androgen-dependent human prostate cancer cell line LNCaP to perform a systematic and broad analysis of the expression, pharmacology, and functionality of vasoactive intestinal peptide (VIP)/pituitary adenylate cyclase-activating peptide (PACAP) receptors. Reverse transcription polymerase chain reaction experiments, together with pharmacological approaches with a set of specific agonists and antagonists, demonstrated the presence of the three VIP/PACAP receptor subtypes (PAC
1, VPAC
1, and VPAC
2) with a major role for VPAC
1, acting through adenylate cyclase (AC) stimulation. An essentially similar pattern was observed by NE differentiated cells (4 days after serum deprivation) in spite of the important morphological changes observed. However, the expression of the prostate-specific antigen (PSA) decreased in NE cells (and increased again by dihydrotestosterone, DHT, treatment). The present demonstration of the induction of NE transdifferentiation in LNCaP cells by increasing concentrations of VIP adds value to previous observations on the role of cAMP in this process, an interesting topic in the comprehension of the molecular changes that are involved in the progression of prostate cancer to androgen independence. |
| doi_str_mv | 10.1016/S0898-6568(01)00199-1 |
| format | Article |
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1, VPAC
1, and VPAC
2) with a major role for VPAC
1, acting through adenylate cyclase (AC) stimulation. An essentially similar pattern was observed by NE differentiated cells (4 days after serum deprivation) in spite of the important morphological changes observed. However, the expression of the prostate-specific antigen (PSA) decreased in NE cells (and increased again by dihydrotestosterone, DHT, treatment). The present demonstration of the induction of NE transdifferentiation in LNCaP cells by increasing concentrations of VIP adds value to previous observations on the role of cAMP in this process, an interesting topic in the comprehension of the molecular changes that are involved in the progression of prostate cancer to androgen independence.</description><identifier>ISSN: 0898-6568</identifier><identifier>EISSN: 1873-3913</identifier><identifier>DOI: 10.1016/S0898-6568(01)00199-1</identifier><identifier>PMID: 11728828</identifier><language>eng</language><publisher>England: Elsevier Inc</publisher><subject>Adenylyl Cyclases - metabolism ; Binding, Competitive ; cAMP ; Cell Differentiation ; Culture Media, Serum-Free ; Cyclic AMP - biosynthesis ; Dose-Response Relationship, Drug ; Humans ; Male ; Neurites - ultrastructure ; Neuroendocrine cells ; Neurons - cytology ; Neurons - metabolism ; Neuropeptides - pharmacology ; Neurosecretory Systems - cytology ; Neurosecretory Systems - metabolism ; PACAP receptors ; Pituitary Adenylate Cyclase-Activating Polypeptide ; Prostate cancer ; Prostatic Neoplasms - genetics ; Prostatic Neoplasms - metabolism ; Prostatic Neoplasms - pathology ; Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide ; Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I ; Receptors, Pituitary Hormone - genetics ; Receptors, Pituitary Hormone - metabolism ; Receptors, Pituitary Hormone - physiology ; Receptors, Vasoactive Intestinal Peptide - genetics ; Receptors, Vasoactive Intestinal Peptide - metabolism ; Receptors, Vasoactive Intestinal Peptide - physiology ; Receptors, Vasoactive Intestinal Peptide, Type II ; Receptors, Vasoactive Intestinal Polypeptide, Type I ; RNA, Neoplasm - biosynthesis ; Transcription, Genetic ; Transdifferentiation ; Tumor Cells, Cultured ; Vasoactive Intestinal Peptide - pharmacology ; VIP receptors</subject><ispartof>Cellular signalling, 2001-12, Vol.13 (12), p.887-894</ispartof><rights>2001 Elsevier Science Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c361t-bcebd0746ea8e254f60fdfaab3396da5054f71fc9ac0029315d8bdc4efd2e0f23</citedby><cites>FETCH-LOGICAL-c361t-bcebd0746ea8e254f60fdfaab3396da5054f71fc9ac0029315d8bdc4efd2e0f23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0898656801001991$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11728828$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Juarranz, Marı́a G.</creatorcontrib><creatorcontrib>Bolaños, Oscar</creatorcontrib><creatorcontrib>Gutiérrez-Cañas, Irene</creatorcontrib><creatorcontrib>Lerner, Ethan A.</creatorcontrib><creatorcontrib>Robberecht, Patrick</creatorcontrib><creatorcontrib>Carmena, Marı́a J.</creatorcontrib><creatorcontrib>Prieto, Juan C.</creatorcontrib><creatorcontrib>Rodrı́guez-Henche, Nieves</creatorcontrib><title>Neuroendocrine differentiation of the LNCaP prostate cancer cell line maintains the expression and function of VIP and PACAP receptors</title><title>Cellular signalling</title><addtitle>Cell Signal</addtitle><description>The molecular mechanisms involved in differentiation of prostate cancer cells to a neuroendocrine (NE) cell phenotype are not well understood. Here we used the androgen-dependent human prostate cancer cell line LNCaP to perform a systematic and broad analysis of the expression, pharmacology, and functionality of vasoactive intestinal peptide (VIP)/pituitary adenylate cyclase-activating peptide (PACAP) receptors. Reverse transcription polymerase chain reaction experiments, together with pharmacological approaches with a set of specific agonists and antagonists, demonstrated the presence of the three VIP/PACAP receptor subtypes (PAC
1, VPAC
1, and VPAC
2) with a major role for VPAC
1, acting through adenylate cyclase (AC) stimulation. An essentially similar pattern was observed by NE differentiated cells (4 days after serum deprivation) in spite of the important morphological changes observed. However, the expression of the prostate-specific antigen (PSA) decreased in NE cells (and increased again by dihydrotestosterone, DHT, treatment). The present demonstration of the induction of NE transdifferentiation in LNCaP cells by increasing concentrations of VIP adds value to previous observations on the role of cAMP in this process, an interesting topic in the comprehension of the molecular changes that are involved in the progression of prostate cancer to androgen independence.</description><subject>Adenylyl Cyclases - metabolism</subject><subject>Binding, Competitive</subject><subject>cAMP</subject><subject>Cell Differentiation</subject><subject>Culture Media, Serum-Free</subject><subject>Cyclic AMP - biosynthesis</subject><subject>Dose-Response Relationship, Drug</subject><subject>Humans</subject><subject>Male</subject><subject>Neurites - ultrastructure</subject><subject>Neuroendocrine cells</subject><subject>Neurons - cytology</subject><subject>Neurons - metabolism</subject><subject>Neuropeptides - pharmacology</subject><subject>Neurosecretory Systems - cytology</subject><subject>Neurosecretory Systems - metabolism</subject><subject>PACAP receptors</subject><subject>Pituitary Adenylate Cyclase-Activating Polypeptide</subject><subject>Prostate cancer</subject><subject>Prostatic Neoplasms - genetics</subject><subject>Prostatic Neoplasms - metabolism</subject><subject>Prostatic Neoplasms - pathology</subject><subject>Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide</subject><subject>Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I</subject><subject>Receptors, Pituitary Hormone - genetics</subject><subject>Receptors, Pituitary Hormone - metabolism</subject><subject>Receptors, Pituitary Hormone - physiology</subject><subject>Receptors, Vasoactive Intestinal Peptide - genetics</subject><subject>Receptors, Vasoactive Intestinal Peptide - metabolism</subject><subject>Receptors, Vasoactive Intestinal Peptide - physiology</subject><subject>Receptors, Vasoactive Intestinal Peptide, Type II</subject><subject>Receptors, Vasoactive Intestinal Polypeptide, Type I</subject><subject>RNA, Neoplasm - biosynthesis</subject><subject>Transcription, Genetic</subject><subject>Transdifferentiation</subject><subject>Tumor Cells, Cultured</subject><subject>Vasoactive Intestinal Peptide - pharmacology</subject><subject>VIP receptors</subject><issn>0898-6568</issn><issn>1873-3913</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFPGzEQha0KVALtT2jlE4LDFs86u-s9VVEELVIEkQq9Wl57rLpKvKntRfQP8LvxJgGOHCxLo-_N6L1HyBdg34BBffGLiVYUdVWLMwbnjEHbFvCBTEA0vOAt8AMyeUWOyHGMfzNUsbr8SI4AmlKIUkzI0w0OoUdveh2cR2qctRjQJ6eS6z3tLU1_kC5u5mpJN6GPSSWkWnmNgWpcrehqlK2V8ym_uKXxcRMwxlGvvKF28Ppl2e_r5Xa2nM1nSxpQ4yb1IX4ih1atIn7e_yfk_urybv6zWNz-uJ7PFoXmNaSi09gZ1kxrVALLamprZo1VquO8rY2qWB41YHWrNGNly6EyojN6itaUyGzJT8jpbm-28m_AmOTaxdGG8tgPUTYlh2nDWQarHaiz5xjQyk1waxX-S2ByLEBuC5BjupKB3BYgIeu-7g8M3RrNm2qfeAa-7wDMNh8cBhm1wxyncTmNJE3v3jnxDEwmmDg</recordid><startdate>20011201</startdate><enddate>20011201</enddate><creator>Juarranz, Marı́a G.</creator><creator>Bolaños, Oscar</creator><creator>Gutiérrez-Cañas, Irene</creator><creator>Lerner, Ethan A.</creator><creator>Robberecht, Patrick</creator><creator>Carmena, Marı́a J.</creator><creator>Prieto, Juan C.</creator><creator>Rodrı́guez-Henche, Nieves</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20011201</creationdate><title>Neuroendocrine differentiation of the LNCaP prostate cancer cell line maintains the expression and function of VIP and PACAP receptors</title><author>Juarranz, Marı́a G. ; Bolaños, Oscar ; Gutiérrez-Cañas, Irene ; Lerner, Ethan A. ; Robberecht, Patrick ; Carmena, Marı́a J. ; Prieto, Juan C. ; Rodrı́guez-Henche, Nieves</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c361t-bcebd0746ea8e254f60fdfaab3396da5054f71fc9ac0029315d8bdc4efd2e0f23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Adenylyl Cyclases - metabolism</topic><topic>Binding, Competitive</topic><topic>cAMP</topic><topic>Cell Differentiation</topic><topic>Culture Media, Serum-Free</topic><topic>Cyclic AMP - biosynthesis</topic><topic>Dose-Response Relationship, Drug</topic><topic>Humans</topic><topic>Male</topic><topic>Neurites - ultrastructure</topic><topic>Neuroendocrine cells</topic><topic>Neurons - cytology</topic><topic>Neurons - metabolism</topic><topic>Neuropeptides - pharmacology</topic><topic>Neurosecretory Systems - cytology</topic><topic>Neurosecretory Systems - metabolism</topic><topic>PACAP receptors</topic><topic>Pituitary Adenylate Cyclase-Activating Polypeptide</topic><topic>Prostate cancer</topic><topic>Prostatic Neoplasms - genetics</topic><topic>Prostatic Neoplasms - metabolism</topic><topic>Prostatic Neoplasms - pathology</topic><topic>Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide</topic><topic>Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I</topic><topic>Receptors, Pituitary Hormone - genetics</topic><topic>Receptors, Pituitary Hormone - metabolism</topic><topic>Receptors, Pituitary Hormone - physiology</topic><topic>Receptors, Vasoactive Intestinal Peptide - genetics</topic><topic>Receptors, Vasoactive Intestinal Peptide - metabolism</topic><topic>Receptors, Vasoactive Intestinal Peptide - physiology</topic><topic>Receptors, Vasoactive Intestinal Peptide, Type II</topic><topic>Receptors, Vasoactive Intestinal Polypeptide, Type I</topic><topic>RNA, Neoplasm - biosynthesis</topic><topic>Transcription, Genetic</topic><topic>Transdifferentiation</topic><topic>Tumor Cells, Cultured</topic><topic>Vasoactive Intestinal Peptide - pharmacology</topic><topic>VIP receptors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Juarranz, Marı́a G.</creatorcontrib><creatorcontrib>Bolaños, Oscar</creatorcontrib><creatorcontrib>Gutiérrez-Cañas, Irene</creatorcontrib><creatorcontrib>Lerner, Ethan A.</creatorcontrib><creatorcontrib>Robberecht, Patrick</creatorcontrib><creatorcontrib>Carmena, Marı́a J.</creatorcontrib><creatorcontrib>Prieto, Juan C.</creatorcontrib><creatorcontrib>Rodrı́guez-Henche, Nieves</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cellular signalling</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Juarranz, Marı́a G.</au><au>Bolaños, Oscar</au><au>Gutiérrez-Cañas, Irene</au><au>Lerner, Ethan A.</au><au>Robberecht, Patrick</au><au>Carmena, Marı́a J.</au><au>Prieto, Juan C.</au><au>Rodrı́guez-Henche, Nieves</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Neuroendocrine differentiation of the LNCaP prostate cancer cell line maintains the expression and function of VIP and PACAP receptors</atitle><jtitle>Cellular signalling</jtitle><addtitle>Cell Signal</addtitle><date>2001-12-01</date><risdate>2001</risdate><volume>13</volume><issue>12</issue><spage>887</spage><epage>894</epage><pages>887-894</pages><issn>0898-6568</issn><eissn>1873-3913</eissn><abstract>The molecular mechanisms involved in differentiation of prostate cancer cells to a neuroendocrine (NE) cell phenotype are not well understood. Here we used the androgen-dependent human prostate cancer cell line LNCaP to perform a systematic and broad analysis of the expression, pharmacology, and functionality of vasoactive intestinal peptide (VIP)/pituitary adenylate cyclase-activating peptide (PACAP) receptors. Reverse transcription polymerase chain reaction experiments, together with pharmacological approaches with a set of specific agonists and antagonists, demonstrated the presence of the three VIP/PACAP receptor subtypes (PAC
1, VPAC
1, and VPAC
2) with a major role for VPAC
1, acting through adenylate cyclase (AC) stimulation. An essentially similar pattern was observed by NE differentiated cells (4 days after serum deprivation) in spite of the important morphological changes observed. However, the expression of the prostate-specific antigen (PSA) decreased in NE cells (and increased again by dihydrotestosterone, DHT, treatment). The present demonstration of the induction of NE transdifferentiation in LNCaP cells by increasing concentrations of VIP adds value to previous observations on the role of cAMP in this process, an interesting topic in the comprehension of the molecular changes that are involved in the progression of prostate cancer to androgen independence.</abstract><cop>England</cop><pub>Elsevier Inc</pub><pmid>11728828</pmid><doi>10.1016/S0898-6568(01)00199-1</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0898-6568 |
| ispartof | Cellular signalling, 2001-12, Vol.13 (12), p.887-894 |
| issn | 0898-6568 1873-3913 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_72314730 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Adenylyl Cyclases - metabolism Binding, Competitive cAMP Cell Differentiation Culture Media, Serum-Free Cyclic AMP - biosynthesis Dose-Response Relationship, Drug Humans Male Neurites - ultrastructure Neuroendocrine cells Neurons - cytology Neurons - metabolism Neuropeptides - pharmacology Neurosecretory Systems - cytology Neurosecretory Systems - metabolism PACAP receptors Pituitary Adenylate Cyclase-Activating Polypeptide Prostate cancer Prostatic Neoplasms - genetics Prostatic Neoplasms - metabolism Prostatic Neoplasms - pathology Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I Receptors, Pituitary Hormone - genetics Receptors, Pituitary Hormone - metabolism Receptors, Pituitary Hormone - physiology Receptors, Vasoactive Intestinal Peptide - genetics Receptors, Vasoactive Intestinal Peptide - metabolism Receptors, Vasoactive Intestinal Peptide - physiology Receptors, Vasoactive Intestinal Peptide, Type II Receptors, Vasoactive Intestinal Polypeptide, Type I RNA, Neoplasm - biosynthesis Transcription, Genetic Transdifferentiation Tumor Cells, Cultured Vasoactive Intestinal Peptide - pharmacology VIP receptors |
| title | Neuroendocrine differentiation of the LNCaP prostate cancer cell line maintains the expression and function of VIP and PACAP receptors |
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