Immunohistochemical analysis of E-cadherin, α-catenin, β-catenin, γ-catenin, and neural cell adhesion molecule (NCAM) in chordoma
Aims—the epithelioid features seen in chordoma are unique among mesenchymal tumours. However, no detailed analysis regarding cell–cell communication has been conducted in this epithelioid tumour. The aims of this study were to investigate cell–cell communication in chordoma. Methods—By means of immu...
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| Veröffentlicht in: | Journal of clinical pathology 2001-12, Vol.54 (12), p.945-950 |
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| creator | Naka, T Oda, Y Iwamoto, Y Shinohara, N Chuman, H Fukui, M Tsuneyoshi, M |
| description | Aims—the epithelioid features seen in chordoma are unique among mesenchymal tumours. However, no detailed analysis regarding cell–cell communication has been conducted in this epithelioid tumour. The aims of this study were to investigate cell–cell communication in chordoma. Methods—By means of immunohistochemical techniques that incorporated a panel of monoclonal antibodies against cell adhesion molecules (CAMs), including E-cadherin, α-catenin, β-catenin, γ-catenin, and neural cell adhesion molecule (NCAM), the expression of CAMs was studied in 15 specimens of chordoma and eight specimens of chondrosarcoma. Results—Most chordoma specimens showed some positive immunoreactivity for all the CAMs examined. For the various CAMs investigated, between two and five cases showed diffuse immunoreactions, indicating well preserved expression. Well preserved expression of all the CAMs examined was limited to only one case, thus indicating that the expression of CAMs was decreased in most of the chordoma specimens; however, no significant correlation was found between the decreased expression of CAMs and the histological grade of malignancy, cellular growth pattern, or clinical parameters in chordoma. In chondrosarcoma, only a few specimens showed positive immunoreactivity for CAMs and the expression of E-cadherin, β-catenin, γ-catenin, and NCAM was seen more frequently in the chordoma specimens than in the chondrosarcoma specimens. Conclusions—These results suggest that the expression of CAMs is associated with the formation and maintenance of chordoma tissue architecture, just as it is in other epithelial tumours or normal tissue. Immunohistochemistry for CAMs was found to be of diagnostic value for discriminating chordoma from chondrosarcoma, and these markers could be used along with the cytokeratins, which are already used for this purpose. |
| doi_str_mv | 10.1136/jcp.54.12.945 |
| format | Article |
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However, no detailed analysis regarding cell–cell communication has been conducted in this epithelioid tumour. The aims of this study were to investigate cell–cell communication in chordoma. Methods—By means of immunohistochemical techniques that incorporated a panel of monoclonal antibodies against cell adhesion molecules (CAMs), including E-cadherin, α-catenin, β-catenin, γ-catenin, and neural cell adhesion molecule (NCAM), the expression of CAMs was studied in 15 specimens of chordoma and eight specimens of chondrosarcoma. Results—Most chordoma specimens showed some positive immunoreactivity for all the CAMs examined. For the various CAMs investigated, between two and five cases showed diffuse immunoreactions, indicating well preserved expression. Well preserved expression of all the CAMs examined was limited to only one case, thus indicating that the expression of CAMs was decreased in most of the chordoma specimens; however, no significant correlation was found between the decreased expression of CAMs and the histological grade of malignancy, cellular growth pattern, or clinical parameters in chordoma. In chondrosarcoma, only a few specimens showed positive immunoreactivity for CAMs and the expression of E-cadherin, β-catenin, γ-catenin, and NCAM was seen more frequently in the chordoma specimens than in the chondrosarcoma specimens. Conclusions—These results suggest that the expression of CAMs is associated with the formation and maintenance of chordoma tissue architecture, just as it is in other epithelial tumours or normal tissue. Immunohistochemistry for CAMs was found to be of diagnostic value for discriminating chordoma from chondrosarcoma, and these markers could be used along with the cytokeratins, which are already used for this purpose.</description><identifier>ISSN: 0021-9746</identifier><identifier>EISSN: 1472-4146</identifier><identifier>DOI: 10.1136/jcp.54.12.945</identifier><identifier>PMID: 11729215</identifier><identifier>CODEN: JCPAAK</identifier><language>eng</language><publisher>London: BMJ Publishing Group Ltd and Association of Clinical Pathologists</publisher><subject>Adult ; Aged ; alpha Catenin ; beta Catenin ; Biological and medical sciences ; Biomarkers, Tumor - analysis ; Bone Neoplasms - chemistry ; Cadherins - analysis ; catenin ; Cell adhesion & migration ; Cell Adhesion Molecules - analysis ; Chondrosarcoma - chemistry ; chordoma ; Chordoma - chemistry ; Cytoskeletal Proteins - analysis ; Desmoplakins ; Diagnosis, Differential ; Diseases of the osteoarticular system ; E-cadherin ; Female ; gamma Catenin ; Humans ; Immunohistochemistry - methods ; Laboratories ; Male ; Medical sciences ; Microwaves ; Middle Aged ; Morphology ; neural cell adhesion molecule ; Neural Cell Adhesion Molecules - analysis ; Trans-Activators ; Tumors ; Tumors of striated muscle and skeleton</subject><ispartof>Journal of clinical pathology, 2001-12, Vol.54 (12), p.945-950</ispartof><rights>2002 INIST-CNRS</rights><rights>COPYRIGHT © 2001 Journal of Clinical Pathology 2001</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14123416$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11729215$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Naka, T</creatorcontrib><creatorcontrib>Oda, Y</creatorcontrib><creatorcontrib>Iwamoto, Y</creatorcontrib><creatorcontrib>Shinohara, N</creatorcontrib><creatorcontrib>Chuman, H</creatorcontrib><creatorcontrib>Fukui, M</creatorcontrib><creatorcontrib>Tsuneyoshi, M</creatorcontrib><title>Immunohistochemical analysis of E-cadherin, α-catenin, β-catenin, γ-catenin, and neural cell adhesion molecule (NCAM) in chordoma</title><title>Journal of clinical pathology</title><addtitle>J Clin Pathol</addtitle><description>Aims—the epithelioid features seen in chordoma are unique among mesenchymal tumours. However, no detailed analysis regarding cell–cell communication has been conducted in this epithelioid tumour. The aims of this study were to investigate cell–cell communication in chordoma. Methods—By means of immunohistochemical techniques that incorporated a panel of monoclonal antibodies against cell adhesion molecules (CAMs), including E-cadherin, α-catenin, β-catenin, γ-catenin, and neural cell adhesion molecule (NCAM), the expression of CAMs was studied in 15 specimens of chordoma and eight specimens of chondrosarcoma. Results—Most chordoma specimens showed some positive immunoreactivity for all the CAMs examined. For the various CAMs investigated, between two and five cases showed diffuse immunoreactions, indicating well preserved expression. Well preserved expression of all the CAMs examined was limited to only one case, thus indicating that the expression of CAMs was decreased in most of the chordoma specimens; however, no significant correlation was found between the decreased expression of CAMs and the histological grade of malignancy, cellular growth pattern, or clinical parameters in chordoma. In chondrosarcoma, only a few specimens showed positive immunoreactivity for CAMs and the expression of E-cadherin, β-catenin, γ-catenin, and NCAM was seen more frequently in the chordoma specimens than in the chondrosarcoma specimens. Conclusions—These results suggest that the expression of CAMs is associated with the formation and maintenance of chordoma tissue architecture, just as it is in other epithelial tumours or normal tissue. Immunohistochemistry for CAMs was found to be of diagnostic value for discriminating chordoma from chondrosarcoma, and these markers could be used along with the cytokeratins, which are already used for this purpose.</description><subject>Adult</subject><subject>Aged</subject><subject>alpha Catenin</subject><subject>beta Catenin</subject><subject>Biological and medical sciences</subject><subject>Biomarkers, Tumor - analysis</subject><subject>Bone Neoplasms - chemistry</subject><subject>Cadherins - analysis</subject><subject>catenin</subject><subject>Cell adhesion & migration</subject><subject>Cell Adhesion Molecules - analysis</subject><subject>Chondrosarcoma - chemistry</subject><subject>chordoma</subject><subject>Chordoma - chemistry</subject><subject>Cytoskeletal Proteins - analysis</subject><subject>Desmoplakins</subject><subject>Diagnosis, Differential</subject><subject>Diseases of the osteoarticular system</subject><subject>E-cadherin</subject><subject>Female</subject><subject>gamma Catenin</subject><subject>Humans</subject><subject>Immunohistochemistry - methods</subject><subject>Laboratories</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Microwaves</subject><subject>Middle Aged</subject><subject>Morphology</subject><subject>neural cell adhesion molecule</subject><subject>Neural Cell Adhesion Molecules - analysis</subject><subject>Trans-Activators</subject><subject>Tumors</subject><subject>Tumors of striated muscle and skeleton</subject><issn>0021-9746</issn><issn>1472-4146</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNpdkc9uEzEQxi0EomngyBVZQiCQ2OAZe-3dYxsVqFTCgT9Xy_HaisOuHdZZid77QsB78EwYGijiNN9ofvNp9A0hD4AtALh8sbW7RS0WgItW1LfIDITCSoCQt8mMMYSqVUIekeOct4wBV8DvkiMAhS1CPSNX58MwxbQJeZ_sxg3Bmp6aaPrLHDJNnp5V1nQbN4b4nP74Wpq9i7_1t3_09xttYkejm8ZiY11fvMpyDinSIfXOTr2jT1fLkzfPaIjUbtLYpcHcI3e86bO7f6hz8uHl2fvl6-ri7avz5clFFTjj-0p05RSmnMfGqJojbxD9GkH4VrXMcNk1XFiQbs2QCSOVsd56x73BzjZC8Tl5cu27G9PnyeW9HkL-daWJLk1ZK-QgauAFfPQfuE3TWFLJGiUKZBILOycPD9S0Hlynd2MYzHip_6RbgMcHwOSSqx9NtCHfcAKQC5CFq6658gX35e_cjJ-0VFzVevVxqVftqWjY6Tvd8J_bSZki</recordid><startdate>20011201</startdate><enddate>20011201</enddate><creator>Naka, T</creator><creator>Oda, Y</creator><creator>Iwamoto, Y</creator><creator>Shinohara, N</creator><creator>Chuman, H</creator><creator>Fukui, M</creator><creator>Tsuneyoshi, M</creator><general>BMJ Publishing Group Ltd and Association of Clinical Pathologists</general><general>BMJ</general><general>BMJ Publishing Group LTD</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88I</scope><scope>8AF</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BTHHO</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20011201</creationdate><title>Immunohistochemical analysis of E-cadherin, α-catenin, β-catenin, γ-catenin, and neural cell adhesion molecule (NCAM) in chordoma</title><author>Naka, T ; Oda, Y ; Iwamoto, Y ; Shinohara, N ; Chuman, H ; Fukui, M ; Tsuneyoshi, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-i303t-4dcad07ef28a75323822fb214f9790a36d834c16eb0204a67acfcfe3fa2dc8473</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Adult</topic><topic>Aged</topic><topic>alpha Catenin</topic><topic>beta Catenin</topic><topic>Biological and medical sciences</topic><topic>Biomarkers, Tumor - analysis</topic><topic>Bone Neoplasms - chemistry</topic><topic>Cadherins - analysis</topic><topic>catenin</topic><topic>Cell adhesion & migration</topic><topic>Cell Adhesion Molecules - analysis</topic><topic>Chondrosarcoma - chemistry</topic><topic>chordoma</topic><topic>Chordoma - chemistry</topic><topic>Cytoskeletal Proteins - analysis</topic><topic>Desmoplakins</topic><topic>Diagnosis, Differential</topic><topic>Diseases of the osteoarticular system</topic><topic>E-cadherin</topic><topic>Female</topic><topic>gamma Catenin</topic><topic>Humans</topic><topic>Immunohistochemistry - methods</topic><topic>Laboratories</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Microwaves</topic><topic>Middle Aged</topic><topic>Morphology</topic><topic>neural cell adhesion molecule</topic><topic>Neural Cell Adhesion Molecules - analysis</topic><topic>Trans-Activators</topic><topic>Tumors</topic><topic>Tumors of striated muscle and skeleton</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Naka, T</creatorcontrib><creatorcontrib>Oda, Y</creatorcontrib><creatorcontrib>Iwamoto, Y</creatorcontrib><creatorcontrib>Shinohara, N</creatorcontrib><creatorcontrib>Chuman, H</creatorcontrib><creatorcontrib>Fukui, M</creatorcontrib><creatorcontrib>Tsuneyoshi, M</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>BMJ Journals</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of clinical pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Naka, T</au><au>Oda, Y</au><au>Iwamoto, Y</au><au>Shinohara, N</au><au>Chuman, H</au><au>Fukui, M</au><au>Tsuneyoshi, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunohistochemical analysis of E-cadherin, α-catenin, β-catenin, γ-catenin, and neural cell adhesion molecule (NCAM) in chordoma</atitle><jtitle>Journal of clinical pathology</jtitle><addtitle>J Clin Pathol</addtitle><date>2001-12-01</date><risdate>2001</risdate><volume>54</volume><issue>12</issue><spage>945</spage><epage>950</epage><pages>945-950</pages><issn>0021-9746</issn><eissn>1472-4146</eissn><coden>JCPAAK</coden><abstract>Aims—the epithelioid features seen in chordoma are unique among mesenchymal tumours. However, no detailed analysis regarding cell–cell communication has been conducted in this epithelioid tumour. The aims of this study were to investigate cell–cell communication in chordoma. Methods—By means of immunohistochemical techniques that incorporated a panel of monoclonal antibodies against cell adhesion molecules (CAMs), including E-cadherin, α-catenin, β-catenin, γ-catenin, and neural cell adhesion molecule (NCAM), the expression of CAMs was studied in 15 specimens of chordoma and eight specimens of chondrosarcoma. Results—Most chordoma specimens showed some positive immunoreactivity for all the CAMs examined. For the various CAMs investigated, between two and five cases showed diffuse immunoreactions, indicating well preserved expression. Well preserved expression of all the CAMs examined was limited to only one case, thus indicating that the expression of CAMs was decreased in most of the chordoma specimens; however, no significant correlation was found between the decreased expression of CAMs and the histological grade of malignancy, cellular growth pattern, or clinical parameters in chordoma. In chondrosarcoma, only a few specimens showed positive immunoreactivity for CAMs and the expression of E-cadherin, β-catenin, γ-catenin, and NCAM was seen more frequently in the chordoma specimens than in the chondrosarcoma specimens. Conclusions—These results suggest that the expression of CAMs is associated with the formation and maintenance of chordoma tissue architecture, just as it is in other epithelial tumours or normal tissue. Immunohistochemistry for CAMs was found to be of diagnostic value for discriminating chordoma from chondrosarcoma, and these markers could be used along with the cytokeratins, which are already used for this purpose.</abstract><cop>London</cop><pub>BMJ Publishing Group Ltd and Association of Clinical Pathologists</pub><pmid>11729215</pmid><doi>10.1136/jcp.54.12.945</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adult Aged alpha Catenin beta Catenin Biological and medical sciences Biomarkers, Tumor - analysis Bone Neoplasms - chemistry Cadherins - analysis catenin Cell adhesion & migration Cell Adhesion Molecules - analysis Chondrosarcoma - chemistry chordoma Chordoma - chemistry Cytoskeletal Proteins - analysis Desmoplakins Diagnosis, Differential Diseases of the osteoarticular system E-cadherin Female gamma Catenin Humans Immunohistochemistry - methods Laboratories Male Medical sciences Microwaves Middle Aged Morphology neural cell adhesion molecule Neural Cell Adhesion Molecules - analysis Trans-Activators Tumors Tumors of striated muscle and skeleton |
| title | Immunohistochemical analysis of E-cadherin, α-catenin, β-catenin, γ-catenin, and neural cell adhesion molecule (NCAM) in chordoma |
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