Molecular characterization of Cryptosporidium isolates obtained from human and bovine infections in Japan
Cryptosporidium oocysts, morphologically identified as Cryptosporidium parvum, were isolated from 22 human and 14 bovine cases in Japan, and were genotyped by means of a PCR/RFLP analysis of the polythreonine gene. DNA profiles of human isolates gave three distinct genotypes, namely an anthroponotic...
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| Veröffentlicht in: | Parasitology research (1987) 2001-11, Vol.87 (11), p.950-955 |
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| container_title | Parasitology research (1987) |
| container_volume | 87 |
| creator | YAGITA, Kenji IZUMIYAMA, Shinji TACHIBANA, Hiroshi MASUDA, Gouta ISEKI, Motohiro FURUYA, Koji KAMEOKA, Yosuke KUROKI, Toshiro ITAGAKI, Tadashi ENDO, Takuro |
| description | Cryptosporidium oocysts, morphologically identified as Cryptosporidium parvum, were isolated from 22 human and 14 bovine cases in Japan, and were genotyped by means of a PCR/RFLP analysis of the polythreonine gene. DNA profiles of human isolates gave three distinct genotypes, namely an anthroponotic genotype 1, zoonotic genotype 2 and a new genotype. Isolates from bovine samples gave zoonotic genotype 2. The unusual genotype of Cryptosporidium was isolated from the feces of three immunologically healthy adults, and was further characterized by the sequence analysis of the 18S rRNA gene. The third genotype was identified as Crypto sporidium meleagridis, demonstrating that C. meleagridis, which occurs worldwide, has the potential to infect humans regardless of their immunological condition. |
| doi_str_mv | 10.1007/s004360100480 |
| format | Article |
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DNA profiles of human isolates gave three distinct genotypes, namely an anthroponotic genotype 1, zoonotic genotype 2 and a new genotype. Isolates from bovine samples gave zoonotic genotype 2. The unusual genotype of Cryptosporidium was isolated from the feces of three immunologically healthy adults, and was further characterized by the sequence analysis of the 18S rRNA gene. The third genotype was identified as Crypto sporidium meleagridis, demonstrating that C. meleagridis, which occurs worldwide, has the potential to infect humans regardless of their immunological condition.</description><identifier>ISSN: 0932-0113</identifier><identifier>EISSN: 1432-1955</identifier><identifier>DOI: 10.1007/s004360100480</identifier><identifier>PMID: 11728022</identifier><identifier>CODEN: PARREZ</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Animals ; Base Sequence ; Biological and medical sciences ; Cattle ; Cryptosporidiosis - parasitology ; Cryptosporidiosis - veterinary ; Cryptosporidium parvum - classification ; Cryptosporidium parvum - genetics ; Cryptosporidium parvum - isolation & purification ; DNA Primers - chemistry ; DNA, Protozoan - analysis ; Fundamental and applied biological sciences. Psychology ; Genetics of eukaryotes. Biological and molecular evolution ; Genotype ; Humans ; Immunocompetence ; Immunocompromised Host ; Molecular Sequence Data ; Polymerase Chain Reaction ; Polymorphism, Restriction Fragment Length ; Population genetics, reproduction patterns ; Protozoa ; RNA, Protozoan - analysis ; RNA, Ribosomal - genetics ; Sequence Homology, Nucleic Acid ; Threonine - genetics</subject><ispartof>Parasitology research (1987), 2001-11, Vol.87 (11), p.950-955</ispartof><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c385t-f2f4dc4ed37230626169b8c3bee65f631bbef1a55f7341733b037da6c23ebac63</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14128558$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11728022$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>YAGITA, Kenji</creatorcontrib><creatorcontrib>IZUMIYAMA, Shinji</creatorcontrib><creatorcontrib>TACHIBANA, Hiroshi</creatorcontrib><creatorcontrib>MASUDA, Gouta</creatorcontrib><creatorcontrib>ISEKI, Motohiro</creatorcontrib><creatorcontrib>FURUYA, Koji</creatorcontrib><creatorcontrib>KAMEOKA, Yosuke</creatorcontrib><creatorcontrib>KUROKI, Toshiro</creatorcontrib><creatorcontrib>ITAGAKI, Tadashi</creatorcontrib><creatorcontrib>ENDO, Takuro</creatorcontrib><title>Molecular characterization of Cryptosporidium isolates obtained from human and bovine infections in Japan</title><title>Parasitology research (1987)</title><addtitle>Parasitol Res</addtitle><description>Cryptosporidium oocysts, morphologically identified as Cryptosporidium parvum, were isolated from 22 human and 14 bovine cases in Japan, and were genotyped by means of a PCR/RFLP analysis of the polythreonine gene. DNA profiles of human isolates gave three distinct genotypes, namely an anthroponotic genotype 1, zoonotic genotype 2 and a new genotype. Isolates from bovine samples gave zoonotic genotype 2. The unusual genotype of Cryptosporidium was isolated from the feces of three immunologically healthy adults, and was further characterized by the sequence analysis of the 18S rRNA gene. The third genotype was identified as Crypto sporidium meleagridis, demonstrating that C. meleagridis, which occurs worldwide, has the potential to infect humans regardless of their immunological condition.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cattle</subject><subject>Cryptosporidiosis - parasitology</subject><subject>Cryptosporidiosis - veterinary</subject><subject>Cryptosporidium parvum - classification</subject><subject>Cryptosporidium parvum - genetics</subject><subject>Cryptosporidium parvum - isolation & purification</subject><subject>DNA Primers - chemistry</subject><subject>DNA, Protozoan - analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetics of eukaryotes. Biological and molecular evolution</subject><subject>Genotype</subject><subject>Humans</subject><subject>Immunocompetence</subject><subject>Immunocompromised Host</subject><subject>Molecular Sequence Data</subject><subject>Polymerase Chain Reaction</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>Population genetics, reproduction patterns</subject><subject>Protozoa</subject><subject>RNA, Protozoan - analysis</subject><subject>RNA, Ribosomal - genetics</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>Threonine - genetics</subject><issn>0932-0113</issn><issn>1432-1955</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkM1LwzAYh4Mobk6PXiUXvVXz3e4ow08mXvRc3qQJi7RNTVph_vV2rDA8vQ8vD8_hh9AlJbeUkPwuESK4IiOLghyhORWcZXQp5TGak-XIhFI-Q2cpfRFCcyXEKZpRmrOCMDZH_i3U1gw1RGw2EMH0Nvpf6H1ocXB4FbddH1IXoq_80GCfQg29TTjoHnxrK-xiaPBmaKDF0FZYh5_xjX3rrNlF0oj4FTpoz9GJgzrZi-ku0Ofjw8fqOVu_P72s7teZ4YXsM8ecqIywFc8ZJ4opqpa6MFxbq6RTnGptHQUpXc4FzTnXhOcVKMO41WAUX6CbfbeL4XuwqS8bn4yta2htGFI5ZinL-U7M9qKJIaVoXdlF30DclpSUu23Lf9uO_tUUHnRjq4M9jTkK15MAyUDtIrTGp4MnKCukLPgfBeqCwg</recordid><startdate>20011101</startdate><enddate>20011101</enddate><creator>YAGITA, Kenji</creator><creator>IZUMIYAMA, Shinji</creator><creator>TACHIBANA, Hiroshi</creator><creator>MASUDA, Gouta</creator><creator>ISEKI, Motohiro</creator><creator>FURUYA, Koji</creator><creator>KAMEOKA, Yosuke</creator><creator>KUROKI, Toshiro</creator><creator>ITAGAKI, Tadashi</creator><creator>ENDO, Takuro</creator><general>Springer</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20011101</creationdate><title>Molecular characterization of Cryptosporidium isolates obtained from human and bovine infections in Japan</title><author>YAGITA, Kenji ; IZUMIYAMA, Shinji ; TACHIBANA, Hiroshi ; MASUDA, Gouta ; ISEKI, Motohiro ; FURUYA, Koji ; KAMEOKA, Yosuke ; KUROKI, Toshiro ; ITAGAKI, Tadashi ; ENDO, Takuro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c385t-f2f4dc4ed37230626169b8c3bee65f631bbef1a55f7341733b037da6c23ebac63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cattle</topic><topic>Cryptosporidiosis - parasitology</topic><topic>Cryptosporidiosis - veterinary</topic><topic>Cryptosporidium parvum - classification</topic><topic>Cryptosporidium parvum - genetics</topic><topic>Cryptosporidium parvum - isolation & purification</topic><topic>DNA Primers - chemistry</topic><topic>DNA, Protozoan - analysis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetics of eukaryotes. Biological and molecular evolution</topic><topic>Genotype</topic><topic>Humans</topic><topic>Immunocompetence</topic><topic>Immunocompromised Host</topic><topic>Molecular Sequence Data</topic><topic>Polymerase Chain Reaction</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>Population genetics, reproduction patterns</topic><topic>Protozoa</topic><topic>RNA, Protozoan - analysis</topic><topic>RNA, Ribosomal - genetics</topic><topic>Sequence Homology, Nucleic Acid</topic><topic>Threonine - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>YAGITA, Kenji</creatorcontrib><creatorcontrib>IZUMIYAMA, Shinji</creatorcontrib><creatorcontrib>TACHIBANA, Hiroshi</creatorcontrib><creatorcontrib>MASUDA, Gouta</creatorcontrib><creatorcontrib>ISEKI, Motohiro</creatorcontrib><creatorcontrib>FURUYA, Koji</creatorcontrib><creatorcontrib>KAMEOKA, Yosuke</creatorcontrib><creatorcontrib>KUROKI, Toshiro</creatorcontrib><creatorcontrib>ITAGAKI, Tadashi</creatorcontrib><creatorcontrib>ENDO, Takuro</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Parasitology research (1987)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>YAGITA, Kenji</au><au>IZUMIYAMA, Shinji</au><au>TACHIBANA, Hiroshi</au><au>MASUDA, Gouta</au><au>ISEKI, Motohiro</au><au>FURUYA, Koji</au><au>KAMEOKA, Yosuke</au><au>KUROKI, Toshiro</au><au>ITAGAKI, Tadashi</au><au>ENDO, Takuro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular characterization of Cryptosporidium isolates obtained from human and bovine infections in Japan</atitle><jtitle>Parasitology research (1987)</jtitle><addtitle>Parasitol Res</addtitle><date>2001-11-01</date><risdate>2001</risdate><volume>87</volume><issue>11</issue><spage>950</spage><epage>955</epage><pages>950-955</pages><issn>0932-0113</issn><eissn>1432-1955</eissn><coden>PARREZ</coden><abstract>Cryptosporidium oocysts, morphologically identified as Cryptosporidium parvum, were isolated from 22 human and 14 bovine cases in Japan, and were genotyped by means of a PCR/RFLP analysis of the polythreonine gene. DNA profiles of human isolates gave three distinct genotypes, namely an anthroponotic genotype 1, zoonotic genotype 2 and a new genotype. Isolates from bovine samples gave zoonotic genotype 2. The unusual genotype of Cryptosporidium was isolated from the feces of three immunologically healthy adults, and was further characterized by the sequence analysis of the 18S rRNA gene. The third genotype was identified as Crypto sporidium meleagridis, demonstrating that C. meleagridis, which occurs worldwide, has the potential to infect humans regardless of their immunological condition.</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>11728022</pmid><doi>10.1007/s004360100480</doi><tpages>6</tpages></addata></record> |
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| subjects | Animals Base Sequence Biological and medical sciences Cattle Cryptosporidiosis - parasitology Cryptosporidiosis - veterinary Cryptosporidium parvum - classification Cryptosporidium parvum - genetics Cryptosporidium parvum - isolation & purification DNA Primers - chemistry DNA, Protozoan - analysis Fundamental and applied biological sciences. Psychology Genetics of eukaryotes. Biological and molecular evolution Genotype Humans Immunocompetence Immunocompromised Host Molecular Sequence Data Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Population genetics, reproduction patterns Protozoa RNA, Protozoan - analysis RNA, Ribosomal - genetics Sequence Homology, Nucleic Acid Threonine - genetics |
| title | Molecular characterization of Cryptosporidium isolates obtained from human and bovine infections in Japan |
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