Sphingosylphosphorylcholine induces Ca(2+)-sensitization of vascular smooth muscle contraction: possible involvement of rho-kinase
Sphingosylphosphorylcholine (SPC), a sphingolipid, concentration-dependently (1-50 microM) induced contraction and slight elevation of the cytosolic Ca(2+) concentration ([Ca(2+)](i)) in smooth muscle of the pig coronary artery, the result being a marked increase in the force/[Ca(2+)](i) ratio. In a...
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| Veröffentlicht in: | FEBS letters 2000-09, Vol.482 (1-2), p.85-90 |
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| creator | Todoroki-Ikeda, N Mizukami, Y Mogami, K Kusuda, T Yamamoto, K Miyake, T Sato, M Suzuki, S Yamagata, H Hokazono, Y Kobayashi, S |
| description | Sphingosylphosphorylcholine (SPC), a sphingolipid, concentration-dependently (1-50 microM) induced contraction and slight elevation of the cytosolic Ca(2+) concentration ([Ca(2+)](i)) in smooth muscle of the pig coronary artery, the result being a marked increase in the force/[Ca(2+)](i) ratio. In alpha-toxin- or beta-escin-permeabilized, but not Triton X-100-permeabilized, vascular strips, SPC induced contraction at constant [Ca(2+)](i) (pCa 6.3) in the absence of GTP, whereas a G-protein-coupled receptor agonist, histamine, required the presence of GTP to induce the contraction. The Rho-kinase blocker, Y-27632 (10 microM) abolished the SPC-induced Ca(2+)-sensitization, without affecting the Ca(2+)-induced contraction. These results suggest that SPC induces Ca(2+)-sensitization of force in vascular smooth muscle, presumably through the activation of Rho-kinase (or a related kinase). |
| doi_str_mv | 10.1016/S0014-5793(00)02046-9 |
| format | Article |
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In alpha-toxin- or beta-escin-permeabilized, but not Triton X-100-permeabilized, vascular strips, SPC induced contraction at constant [Ca(2+)](i) (pCa 6.3) in the absence of GTP, whereas a G-protein-coupled receptor agonist, histamine, required the presence of GTP to induce the contraction. The Rho-kinase blocker, Y-27632 (10 microM) abolished the SPC-induced Ca(2+)-sensitization, without affecting the Ca(2+)-induced contraction. 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In alpha-toxin- or beta-escin-permeabilized, but not Triton X-100-permeabilized, vascular strips, SPC induced contraction at constant [Ca(2+)](i) (pCa 6.3) in the absence of GTP, whereas a G-protein-coupled receptor agonist, histamine, required the presence of GTP to induce the contraction. The Rho-kinase blocker, Y-27632 (10 microM) abolished the SPC-induced Ca(2+)-sensitization, without affecting the Ca(2+)-induced contraction. These results suggest that SPC induces Ca(2+)-sensitization of force in vascular smooth muscle, presumably through the activation of Rho-kinase (or a related kinase).</description><subject>Amides - pharmacology</subject><subject>Animals</subject><subject>Calcium - metabolism</subject><subject>Coronary Vessels - drug effects</subject><subject>Coronary Vessels - physiology</subject><subject>Cytosol - metabolism</subject><subject>Enzyme Inhibitors - pharmacology</subject><subject>Guanosine Triphosphate - metabolism</subject><subject>Histamine - pharmacology</subject><subject>In Vitro Techniques</subject><subject>Intracellular Signaling Peptides and Proteins</subject><subject>Kinetics</subject><subject>Muscle Contraction - drug effects</subject><subject>Muscle Contraction - physiology</subject><subject>Muscle, Smooth, Vascular - drug effects</subject><subject>Muscle, Smooth, Vascular - physiology</subject><subject>Phosphorylcholine - analogs & derivatives</subject><subject>Phosphorylcholine - pharmacology</subject><subject>Potassium - pharmacology</subject><subject>Protein-Serine-Threonine Kinases - metabolism</subject><subject>Pyridines - pharmacology</subject><subject>rho-Associated Kinases</subject><subject>Sphingosine - analogs & derivatives</subject><subject>Sphingosine - pharmacology</subject><subject>Swine</subject><issn>0014-5793</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kE1Lw0AQhveg2Fr9CUpO0iLR_Wo38SbFLyh4qJ7DZjMxq5vdmEkK9egvN8XqYZh5h-d9YYaQM0avGGWL6zWlTMZzlYoppTPKqVzE6QEZ_69H5BjxnQ46YekRGbHBlsx5Mibf66ay_i3g1jVVwKHarTNVcNZDZH3RG8Boqaf8chYjeLSd_dKdDT4KZbTRaHqn2wjrELoqqns0DiITfNdqs6NuoiYg2tztwjbBbaAG3-28bRXiD-s1wgk5LLVDON33CXm9v3tZPsar54en5e0qNkzJLgaRJCbJmVwUaZrLMkmHYS4BpNRzBaXSBc9VXhSlHs7jLJdCldwUoBUVWigxIRe_uU0bPnvALqstGnBOewg9ZooLxgXnA3i-B_u8hiJrWlvrdpv9fU38AL2Mcd4</recordid><startdate>20000929</startdate><enddate>20000929</enddate><creator>Todoroki-Ikeda, N</creator><creator>Mizukami, Y</creator><creator>Mogami, K</creator><creator>Kusuda, T</creator><creator>Yamamoto, K</creator><creator>Miyake, T</creator><creator>Sato, M</creator><creator>Suzuki, S</creator><creator>Yamagata, H</creator><creator>Hokazono, Y</creator><creator>Kobayashi, S</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20000929</creationdate><title>Sphingosylphosphorylcholine induces Ca(2+)-sensitization of vascular smooth muscle contraction: possible involvement of rho-kinase</title><author>Todoroki-Ikeda, N ; 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In alpha-toxin- or beta-escin-permeabilized, but not Triton X-100-permeabilized, vascular strips, SPC induced contraction at constant [Ca(2+)](i) (pCa 6.3) in the absence of GTP, whereas a G-protein-coupled receptor agonist, histamine, required the presence of GTP to induce the contraction. The Rho-kinase blocker, Y-27632 (10 microM) abolished the SPC-induced Ca(2+)-sensitization, without affecting the Ca(2+)-induced contraction. These results suggest that SPC induces Ca(2+)-sensitization of force in vascular smooth muscle, presumably through the activation of Rho-kinase (or a related kinase).</abstract><cop>England</cop><pmid>11018528</pmid><doi>10.1016/S0014-5793(00)02046-9</doi><tpages>6</tpages></addata></record> |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Wiley Free Content; ScienceDirect Journals (5 years ago - present); Alma/SFX Local Collection |
| subjects | Amides - pharmacology Animals Calcium - metabolism Coronary Vessels - drug effects Coronary Vessels - physiology Cytosol - metabolism Enzyme Inhibitors - pharmacology Guanosine Triphosphate - metabolism Histamine - pharmacology In Vitro Techniques Intracellular Signaling Peptides and Proteins Kinetics Muscle Contraction - drug effects Muscle Contraction - physiology Muscle, Smooth, Vascular - drug effects Muscle, Smooth, Vascular - physiology Phosphorylcholine - analogs & derivatives Phosphorylcholine - pharmacology Potassium - pharmacology Protein-Serine-Threonine Kinases - metabolism Pyridines - pharmacology rho-Associated Kinases Sphingosine - analogs & derivatives Sphingosine - pharmacology Swine |
| title | Sphingosylphosphorylcholine induces Ca(2+)-sensitization of vascular smooth muscle contraction: possible involvement of rho-kinase |
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