Molecular Epidemiology of a Multiresistant Pseudomonas aeruginosa Outbreak in a Paediatric Intensive Care Unit
After isolation of multiresistant (MR) Pseudomonas aeruginosa from 3 hospitalized patients in a paediatric intensive care unit (PICU), a prospective surveillance programme was established to detect infected and/or colonized patients in the hospital. Isolates were examined by means of outer membrane...
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| Veröffentlicht in: | Scandinavian journal of infectious diseases 2001, Vol.33 (10), p.738-743 |
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| container_title | Scandinavian journal of infectious diseases |
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| creator | Miranda, Guadalupe Leanos, Blanca Valenzuela, Adriana Silva, Jesus Carrillo, Berta Munoz, Onofre Solorzano, Fortino Marquez, Luis |
| description | After isolation of multiresistant (MR) Pseudomonas aeruginosa from 3 hospitalized patients in a paediatric intensive care unit (PICU), a prospective surveillance programme was established to detect infected and/or colonized patients in the hospital. Isolates were examined by means of outer membrane protein (OMP) profiles, serotyping and DNA genomic analysis using pulsed-field gel electrophoresis (PFGE). Fifty-five P. aeruginosa strains were isolated from 23 hospitalized patients during September and October 1997. The median hospital stay before isolation of P. aeruginosa was 8 d. PFGE demonstrated that the same clone infected 14 patients, 4 of whom were not hospitalized in the PICU. Susceptibility patterns and OMP profiles correlated with PFGE results in 37.8% and 36.4% of cases, respectively. Serotype O11 correlated with pattern A in 77% of cases and serotype O4 correlated with unrelated strains in 75% of cases but did not discriminate between outbreak and unrelated isolates. Extensive investigation of cultures failed to identify a reservoir of P. aeruginosa. PFGE was superior to OMP analysis and serotyping for discriminating between strains. The possible mode of acquisition for most of the patients infected with the same clone was cross-contamination. |
| doi_str_mv | 10.1080/003655401317074527 |
| format | Article |
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Isolates were examined by means of outer membrane protein (OMP) profiles, serotyping and DNA genomic analysis using pulsed-field gel electrophoresis (PFGE). Fifty-five P. aeruginosa strains were isolated from 23 hospitalized patients during September and October 1997. The median hospital stay before isolation of P. aeruginosa was 8 d. PFGE demonstrated that the same clone infected 14 patients, 4 of whom were not hospitalized in the PICU. Susceptibility patterns and OMP profiles correlated with PFGE results in 37.8% and 36.4% of cases, respectively. Serotype O11 correlated with pattern A in 77% of cases and serotype O4 correlated with unrelated strains in 75% of cases but did not discriminate between outbreak and unrelated isolates. Extensive investigation of cultures failed to identify a reservoir of P. aeruginosa. PFGE was superior to OMP analysis and serotyping for discriminating between strains. 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Isolates were examined by means of outer membrane protein (OMP) profiles, serotyping and DNA genomic analysis using pulsed-field gel electrophoresis (PFGE). Fifty-five P. aeruginosa strains were isolated from 23 hospitalized patients during September and October 1997. The median hospital stay before isolation of P. aeruginosa was 8 d. PFGE demonstrated that the same clone infected 14 patients, 4 of whom were not hospitalized in the PICU. Susceptibility patterns and OMP profiles correlated with PFGE results in 37.8% and 36.4% of cases, respectively. Serotype O11 correlated with pattern A in 77% of cases and serotype O4 correlated with unrelated strains in 75% of cases but did not discriminate between outbreak and unrelated isolates. Extensive investigation of cultures failed to identify a reservoir of P. aeruginosa. PFGE was superior to OMP analysis and serotyping for discriminating between strains. The possible mode of acquisition for most of the patients infected with the same clone was cross-contamination.</description><subject>Adolescent</subject><subject>Bacterial diseases</subject><subject>Bacterial Proteins - analysis</subject><subject>Biological and medical sciences</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Cross Infection - diagnosis</subject><subject>Cross Infection - epidemiology</subject><subject>Cross Infection - microbiology</subject><subject>Disease Outbreaks</subject><subject>DNA, Bacterial - analysis</subject><subject>Drug Resistance, Multiple, Bacterial</subject><subject>Electrophoresis, Gel, Pulsed-Field - methods</subject><subject>Human bacterial diseases</subject><subject>Humans</subject><subject>Infant</subject><subject>Infant, Newborn</subject><subject>Infectious diseases</subject><subject>Intensive Care Units, Pediatric</subject><subject>Lipoproteins - analysis</subject><subject>Medical sciences</subject><subject>Miscellaneous</subject><subject>New Mexico - epidemiology</subject><subject>Pseudomonas aeruginosa</subject><subject>Pseudomonas aeruginosa - drug effects</subject><subject>Pseudomonas aeruginosa - genetics</subject><subject>Pseudomonas aeruginosa - isolation & purification</subject><subject>Pseudomonas Infections - diagnosis</subject><subject>Pseudomonas Infections - epidemiology</subject><subject>Serotyping - methods</subject><issn>0036-5548</issn><issn>1651-1980</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFu1DAQhi0EotvCC3BAvsAt4LHjxJHgUK1aqNSqPdBzNEkmrYtjL7YD2rcnq11UIaRyGV--7_fYP2NvQHwAYcRHIVSldSlAQS3qUsv6GVtBpaGAxojnbLUDioUwR-w4pQchRFkp8ZIdAdTSCGVWzF8FR_3sMPKzjR1ossGFuy0PI0d-NbtsIyWbMvrMbxLNQ5iCx8SR4nxnfUjIr-fcRcLv3PrFuUEaLOZoe37hM_lkfxJfYyR-621-xV6M6BK9Ppwn7Pb87Nv6a3F5_eVifXpZ9GVV56JDUqKTlaq6gXqSpdS9FoDQKGhE2Q0NCK1rqo1oDFV6GUaWpLpaNao0oE7Y-33uJoYfM6XcTjb15Bx6CnNqa6kAtG7-C4KRII3agXIP9jGkFGlsN9FOGLctiHZXR_tvHYv09pA-dxMNj8rh_xfg3QHA1KMbI_repkeuBKU07G7_vOesH0Oc8FeIbmgzbl2IfyT15CKf_vLvCV2-75da2ocwR7908dQ7fgPzPbdd</recordid><startdate>2001</startdate><enddate>2001</enddate><creator>Miranda, Guadalupe</creator><creator>Leanos, Blanca</creator><creator>Valenzuela, Adriana</creator><creator>Silva, Jesus</creator><creator>Carrillo, Berta</creator><creator>Munoz, Onofre</creator><creator>Solorzano, Fortino</creator><creator>Marquez, Luis</creator><general>Informa UK Ltd</general><general>Taylor & Francis</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>2001</creationdate><title>Molecular Epidemiology of a Multiresistant Pseudomonas aeruginosa Outbreak in a Paediatric Intensive Care Unit</title><author>Miranda, Guadalupe ; Leanos, Blanca ; Valenzuela, Adriana ; Silva, Jesus ; Carrillo, Berta ; Munoz, Onofre ; Solorzano, Fortino ; Marquez, Luis</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c467t-bae30b2636bdece2425c501a1931904bd910557e78098e6598e824e3b73934813</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Adolescent</topic><topic>Bacterial diseases</topic><topic>Bacterial Proteins - analysis</topic><topic>Biological and medical sciences</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Cross Infection - diagnosis</topic><topic>Cross Infection - epidemiology</topic><topic>Cross Infection - microbiology</topic><topic>Disease Outbreaks</topic><topic>DNA, Bacterial - analysis</topic><topic>Drug Resistance, Multiple, Bacterial</topic><topic>Electrophoresis, Gel, Pulsed-Field - methods</topic><topic>Human bacterial diseases</topic><topic>Humans</topic><topic>Infant</topic><topic>Infant, Newborn</topic><topic>Infectious diseases</topic><topic>Intensive Care Units, Pediatric</topic><topic>Lipoproteins - analysis</topic><topic>Medical sciences</topic><topic>Miscellaneous</topic><topic>New Mexico - epidemiology</topic><topic>Pseudomonas aeruginosa</topic><topic>Pseudomonas aeruginosa - drug effects</topic><topic>Pseudomonas aeruginosa - genetics</topic><topic>Pseudomonas aeruginosa - isolation & purification</topic><topic>Pseudomonas Infections - diagnosis</topic><topic>Pseudomonas Infections - epidemiology</topic><topic>Serotyping - methods</topic><toplevel>online_resources</toplevel><creatorcontrib>Miranda, Guadalupe</creatorcontrib><creatorcontrib>Leanos, Blanca</creatorcontrib><creatorcontrib>Valenzuela, Adriana</creatorcontrib><creatorcontrib>Silva, Jesus</creatorcontrib><creatorcontrib>Carrillo, Berta</creatorcontrib><creatorcontrib>Munoz, Onofre</creatorcontrib><creatorcontrib>Solorzano, Fortino</creatorcontrib><creatorcontrib>Marquez, Luis</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Scandinavian journal of infectious diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Miranda, Guadalupe</au><au>Leanos, Blanca</au><au>Valenzuela, Adriana</au><au>Silva, Jesus</au><au>Carrillo, Berta</au><au>Munoz, Onofre</au><au>Solorzano, Fortino</au><au>Marquez, Luis</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular Epidemiology of a Multiresistant Pseudomonas aeruginosa Outbreak in a Paediatric Intensive Care Unit</atitle><jtitle>Scandinavian journal of infectious diseases</jtitle><addtitle>Scand J Infect Dis</addtitle><date>2001</date><risdate>2001</risdate><volume>33</volume><issue>10</issue><spage>738</spage><epage>743</epage><pages>738-743</pages><issn>0036-5548</issn><eissn>1651-1980</eissn><coden>SJIDB7</coden><abstract>After isolation of multiresistant (MR) Pseudomonas aeruginosa from 3 hospitalized patients in a paediatric intensive care unit (PICU), a prospective surveillance programme was established to detect infected and/or colonized patients in the hospital. Isolates were examined by means of outer membrane protein (OMP) profiles, serotyping and DNA genomic analysis using pulsed-field gel electrophoresis (PFGE). Fifty-five P. aeruginosa strains were isolated from 23 hospitalized patients during September and October 1997. The median hospital stay before isolation of P. aeruginosa was 8 d. PFGE demonstrated that the same clone infected 14 patients, 4 of whom were not hospitalized in the PICU. Susceptibility patterns and OMP profiles correlated with PFGE results in 37.8% and 36.4% of cases, respectively. Serotype O11 correlated with pattern A in 77% of cases and serotype O4 correlated with unrelated strains in 75% of cases but did not discriminate between outbreak and unrelated isolates. Extensive investigation of cultures failed to identify a reservoir of P. aeruginosa. PFGE was superior to OMP analysis and serotyping for discriminating between strains. 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| ispartof | Scandinavian journal of infectious diseases, 2001, Vol.33 (10), p.738-743 |
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| subjects | Adolescent Bacterial diseases Bacterial Proteins - analysis Biological and medical sciences Child Child, Preschool Cross Infection - diagnosis Cross Infection - epidemiology Cross Infection - microbiology Disease Outbreaks DNA, Bacterial - analysis Drug Resistance, Multiple, Bacterial Electrophoresis, Gel, Pulsed-Field - methods Human bacterial diseases Humans Infant Infant, Newborn Infectious diseases Intensive Care Units, Pediatric Lipoproteins - analysis Medical sciences Miscellaneous New Mexico - epidemiology Pseudomonas aeruginosa Pseudomonas aeruginosa - drug effects Pseudomonas aeruginosa - genetics Pseudomonas aeruginosa - isolation & purification Pseudomonas Infections - diagnosis Pseudomonas Infections - epidemiology Serotyping - methods |
| title | Molecular Epidemiology of a Multiresistant Pseudomonas aeruginosa Outbreak in a Paediatric Intensive Care Unit |
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