Mass Spectral Characterization of Lipooligosaccharides from Haemophilus influenzae 2019
Lipooligosaccharide (LOS) glycoforms from Haemophilus influenzae 2019 were profiled using the high-resolution and accurate mass capabilities of Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry. Sequence and linkage for two previously unknown LOS glycoforms were subsequently obtai...
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| Veröffentlicht in: | Biochemistry (Easton) 2000-10, Vol.39 (40), p.12406-12414 |
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| creator | Gaucher, Sara P Cancilla, Mark T Phillips, Nancy J Gibson, Bradford W Leary, Julie A |
| description | Lipooligosaccharide (LOS) glycoforms from Haemophilus influenzae 2019 were profiled using the high-resolution and accurate mass capabilities of Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry. Sequence and linkage for two previously unknown LOS glycoforms were subsequently obtained through MS n analyses on FT-ICR and quadrupole ion trap (qIT) instruments. MS n analysis of negative ion precursors confirmed structural details within the lipid moiety, while CID spectra of sodiated precursor ions provided monosaccharide sequence and linkage for the oligosaccharide portion of the molecule. Results obtained in this study indicate that extensive heterogeneity exists within the oligosaccharide moieties in LOS from H. influenzae 2019. More importantly, the data suggest that additional hexose moieties, which are added onto the LOS, are not simple extensions of one particular core structure but rather that structural isomers with different connectivities are present within the heterogeneous mixture. |
| doi_str_mv | 10.1021/bi001181k |
| format | Article |
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Sequence and linkage for two previously unknown LOS glycoforms were subsequently obtained through MS n analyses on FT-ICR and quadrupole ion trap (qIT) instruments. MS n analysis of negative ion precursors confirmed structural details within the lipid moiety, while CID spectra of sodiated precursor ions provided monosaccharide sequence and linkage for the oligosaccharide portion of the molecule. Results obtained in this study indicate that extensive heterogeneity exists within the oligosaccharide moieties in LOS from H. influenzae 2019. More importantly, the data suggest that additional hexose moieties, which are added onto the LOS, are not simple extensions of one particular core structure but rather that structural isomers with different connectivities are present within the heterogeneous mixture.</description><identifier>ISSN: 0006-2960</identifier><identifier>EISSN: 1520-4995</identifier><identifier>DOI: 10.1021/bi001181k</identifier><identifier>PMID: 11015221</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Carbohydrate Conformation ; Carbohydrate Sequence ; Cyclotrons ; Disaccharides - chemistry ; Fourier Analysis ; Haemophilus influenzae ; Haemophilus influenzae - chemistry ; Haemophilus influenzae - metabolism ; Heptoses - chemistry ; Hexoses - chemistry ; Lipid A - chemistry ; Lipid A - metabolism ; lipooligosaccharides ; Lipopolysaccharides - chemistry ; Lipopolysaccharides - metabolism ; Mass Spectrometry - methods ; Molecular Sequence Data ; Phosphorylation ; Polysaccharides - biosynthesis ; Polysaccharides - chemistry ; Polysaccharides - metabolism ; Sugar Acids - chemistry ; Trisaccharides - chemistry</subject><ispartof>Biochemistry (Easton), 2000-10, Vol.39 (40), p.12406-12414</ispartof><rights>Copyright © 2000 American Chemical Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a380t-a505b3ca63e780a71cc662c57c6ca24ec156ed8800411bdc420b6dcc6aa4a343</citedby><cites>FETCH-LOGICAL-a380t-a505b3ca63e780a71cc662c57c6ca24ec156ed8800411bdc420b6dcc6aa4a343</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/bi001181k$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/bi001181k$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2765,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11015221$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gaucher, Sara P</creatorcontrib><creatorcontrib>Cancilla, Mark T</creatorcontrib><creatorcontrib>Phillips, Nancy J</creatorcontrib><creatorcontrib>Gibson, Bradford W</creatorcontrib><creatorcontrib>Leary, Julie A</creatorcontrib><title>Mass Spectral Characterization of Lipooligosaccharides from Haemophilus influenzae 2019</title><title>Biochemistry (Easton)</title><addtitle>Biochemistry</addtitle><description>Lipooligosaccharide (LOS) glycoforms from Haemophilus influenzae 2019 were profiled using the high-resolution and accurate mass capabilities of Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry. Sequence and linkage for two previously unknown LOS glycoforms were subsequently obtained through MS n analyses on FT-ICR and quadrupole ion trap (qIT) instruments. MS n analysis of negative ion precursors confirmed structural details within the lipid moiety, while CID spectra of sodiated precursor ions provided monosaccharide sequence and linkage for the oligosaccharide portion of the molecule. Results obtained in this study indicate that extensive heterogeneity exists within the oligosaccharide moieties in LOS from H. influenzae 2019. More importantly, the data suggest that additional hexose moieties, which are added onto the LOS, are not simple extensions of one particular core structure but rather that structural isomers with different connectivities are present within the heterogeneous mixture.</description><subject>Carbohydrate Conformation</subject><subject>Carbohydrate Sequence</subject><subject>Cyclotrons</subject><subject>Disaccharides - chemistry</subject><subject>Fourier Analysis</subject><subject>Haemophilus influenzae</subject><subject>Haemophilus influenzae - chemistry</subject><subject>Haemophilus influenzae - metabolism</subject><subject>Heptoses - chemistry</subject><subject>Hexoses - chemistry</subject><subject>Lipid A - chemistry</subject><subject>Lipid A - metabolism</subject><subject>lipooligosaccharides</subject><subject>Lipopolysaccharides - chemistry</subject><subject>Lipopolysaccharides - metabolism</subject><subject>Mass Spectrometry - methods</subject><subject>Molecular Sequence Data</subject><subject>Phosphorylation</subject><subject>Polysaccharides - biosynthesis</subject><subject>Polysaccharides - chemistry</subject><subject>Polysaccharides - metabolism</subject><subject>Sugar Acids - chemistry</subject><subject>Trisaccharides - chemistry</subject><issn>0006-2960</issn><issn>1520-4995</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0E1r3DAQBmBRGppt2kP_QPElgR6czsi2ZB_LkmxKth-Qhe1NjGW5UWJbjmRDk18fhV2SS6CnYZiHmeFl7BPCKQLHr7UFQCzx9g1bYMEhzauqeMsWACBSXgk4ZO9DuIltDjJ_xw4RITqOC7b9QSEkV6PRk6cuWV6TJz0Zbx9osm5IXJus7ehcZ_-6QFrHuW1MSFrv-uSCTO_Ga9vNIbFD281meCCTcMDqAztoqQvm474esc352WZ5ka5_rb4vv61TykqYUiqgqDNNIjOyBJKotRBcF1ILTTw3GgthmrKMnyPWjc451KKJiCinLM-O2Mlu7ejd3WzCpHobtOk6Goybg5I8gyqX8r8QpcRoIcIvO6i9C8GbVo3e9uTvFYJ6Sls9px3t5_3Sue5N8yL38UaQ7oANk_n3PCd_q4TMZKE2v69U-WcFPy9XW7WN_njnSQd142Y_xOxeOfwI_86VQQ</recordid><startdate>20001010</startdate><enddate>20001010</enddate><creator>Gaucher, Sara P</creator><creator>Cancilla, Mark T</creator><creator>Phillips, Nancy J</creator><creator>Gibson, Bradford W</creator><creator>Leary, Julie A</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>20001010</creationdate><title>Mass Spectral Characterization of Lipooligosaccharides from Haemophilus influenzae 2019</title><author>Gaucher, Sara P ; Cancilla, Mark T ; Phillips, Nancy J ; Gibson, Bradford W ; Leary, Julie A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a380t-a505b3ca63e780a71cc662c57c6ca24ec156ed8800411bdc420b6dcc6aa4a343</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Carbohydrate Conformation</topic><topic>Carbohydrate Sequence</topic><topic>Cyclotrons</topic><topic>Disaccharides - chemistry</topic><topic>Fourier Analysis</topic><topic>Haemophilus influenzae</topic><topic>Haemophilus influenzae - chemistry</topic><topic>Haemophilus influenzae - metabolism</topic><topic>Heptoses - chemistry</topic><topic>Hexoses - chemistry</topic><topic>Lipid A - chemistry</topic><topic>Lipid A - metabolism</topic><topic>lipooligosaccharides</topic><topic>Lipopolysaccharides - chemistry</topic><topic>Lipopolysaccharides - metabolism</topic><topic>Mass Spectrometry - methods</topic><topic>Molecular Sequence Data</topic><topic>Phosphorylation</topic><topic>Polysaccharides - biosynthesis</topic><topic>Polysaccharides - chemistry</topic><topic>Polysaccharides - metabolism</topic><topic>Sugar Acids - chemistry</topic><topic>Trisaccharides - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gaucher, Sara P</creatorcontrib><creatorcontrib>Cancilla, Mark T</creatorcontrib><creatorcontrib>Phillips, Nancy J</creatorcontrib><creatorcontrib>Gibson, Bradford W</creatorcontrib><creatorcontrib>Leary, Julie A</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemistry (Easton)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gaucher, Sara P</au><au>Cancilla, Mark T</au><au>Phillips, Nancy J</au><au>Gibson, Bradford W</au><au>Leary, Julie A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mass Spectral Characterization of Lipooligosaccharides from Haemophilus influenzae 2019</atitle><jtitle>Biochemistry (Easton)</jtitle><addtitle>Biochemistry</addtitle><date>2000-10-10</date><risdate>2000</risdate><volume>39</volume><issue>40</issue><spage>12406</spage><epage>12414</epage><pages>12406-12414</pages><issn>0006-2960</issn><eissn>1520-4995</eissn><abstract>Lipooligosaccharide (LOS) glycoforms from Haemophilus influenzae 2019 were profiled using the high-resolution and accurate mass capabilities of Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry. Sequence and linkage for two previously unknown LOS glycoforms were subsequently obtained through MS n analyses on FT-ICR and quadrupole ion trap (qIT) instruments. MS n analysis of negative ion precursors confirmed structural details within the lipid moiety, while CID spectra of sodiated precursor ions provided monosaccharide sequence and linkage for the oligosaccharide portion of the molecule. Results obtained in this study indicate that extensive heterogeneity exists within the oligosaccharide moieties in LOS from H. influenzae 2019. More importantly, the data suggest that additional hexose moieties, which are added onto the LOS, are not simple extensions of one particular core structure but rather that structural isomers with different connectivities are present within the heterogeneous mixture.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>11015221</pmid><doi>10.1021/bi001181k</doi><tpages>9</tpages></addata></record> |
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| subjects | Carbohydrate Conformation Carbohydrate Sequence Cyclotrons Disaccharides - chemistry Fourier Analysis Haemophilus influenzae Haemophilus influenzae - chemistry Haemophilus influenzae - metabolism Heptoses - chemistry Hexoses - chemistry Lipid A - chemistry Lipid A - metabolism lipooligosaccharides Lipopolysaccharides - chemistry Lipopolysaccharides - metabolism Mass Spectrometry - methods Molecular Sequence Data Phosphorylation Polysaccharides - biosynthesis Polysaccharides - chemistry Polysaccharides - metabolism Sugar Acids - chemistry Trisaccharides - chemistry |
| title | Mass Spectral Characterization of Lipooligosaccharides from Haemophilus influenzae 2019 |
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