Multiple Folding Pathways for the P4−P6 RNA Domain

We recently described site-specific pyrene labeling of RNA to monitor Mg2+-dependent equilibrium formation of tertiary structure. Here we extend these studies to follow the folding kinetics of the 160-nucleotide P4−P6 domain of the Tetrahymena group I intron RNA, using stopped-flow fluorescence with...

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Veröffentlicht in:	Biochemistry 2000-10, Vol.39 (40), p.12465-12475
Hauptverfasser:	Silverman, Scott K, Deras, Michael L, Woodson, Sarah A, Scaringe, Stephen A, Cech, Thomas R
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals BASIC BIOLOGICAL SCIENCES BIOCHEMISTRY Cacodylic Acid - chemistry Entropy Enzyme Activation Flow Injection Analysis Hydroxyl Radical Introns Kinetics NATIONAL SYNCHROTRON LIGHT SOURCE NSLS Nucleic Acid Conformation Pyrenes - chemistry RNA RNA, Catalytic - chemistry RNA, Protozoan - chemistry Salts Spectrometry, Fluorescence Synchrotrons Temperature Tetrahymena - enzymology Thionucleotides - chemistry X-Rays
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