Flow Cytometry of Escherichia coli on Microfluidic Devices
Flow cytometry of the bacterium Escherichia coli was demonstrated on a microfabricated fluidic device (microchip). The channels were coated with poly(dimethylacrylamide) to prevent cell adhesion, and the cells were transported electrophoretically by applying potentials to the fluid reservoirs. The c...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2001-11, Vol.73 (21), p.5334-5338 |
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| container_title | Analytical chemistry (Washington) |
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| creator | McClain, Maxine A Culbertson, Christopher T Jacobson, Stephen C Ramsey, J. Michael |
| description | Flow cytometry of the bacterium Escherichia coli was demonstrated on a microfabricated fluidic device (microchip). The channels were coated with poly(dimethylacrylamide) to prevent cell adhesion, and the cells were transported electrophoretically by applying potentials to the fluid reservoirs. The cells were electrophoretically focused at the channel cross and detected by coincident light scattering and fluorescence. The E. coli were labeled with a membrane-permeable nucleic acid stain (Syto15), a membrane-impermeable nucleic acid stain (propidium iodide), or a fluorescein-labeled antibody and counted at rates from 30 to 85 Hz. The observed labeling efficiencies for the dyes and antibody were greater than 94%. |
| doi_str_mv | 10.1021/ac010504v |
| format | Article |
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Michael</creatorcontrib><title>Flow Cytometry of Escherichia coli on Microfluidic Devices</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. Chem</addtitle><description>Flow cytometry of the bacterium Escherichia coli was demonstrated on a microfabricated fluidic device (microchip). The channels were coated with poly(dimethylacrylamide) to prevent cell adhesion, and the cells were transported electrophoretically by applying potentials to the fluid reservoirs. The cells were electrophoretically focused at the channel cross and detected by coincident light scattering and fluorescence. The E. coli were labeled with a membrane-permeable nucleic acid stain (Syto15), a membrane-impermeable nucleic acid stain (propidium iodide), or a fluorescein-labeled antibody and counted at rates from 30 to 85 Hz. The observed labeling efficiencies for the dyes and antibody were greater than 94%.</description><subject>Analysis</subject><subject>Bacteria</subject><subject>Bacteriological methods and techniques used in bacteriology</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Cell Membrane Permeability</subject><subject>Cells</subject><subject>Electrophoresis - methods</subject><subject>Escherichia coli - cytology</subject><subject>Escherichia coli - genetics</subject><subject>Escherichia coli - metabolism</subject><subject>Flow Cytometry - instrumentation</subject><subject>Flow Cytometry - methods</subject><subject>Fluorescent Antibody Technique - methods</subject><subject>Fluorescent Dyes - metabolism</subject><subject>Fundamental and applied biological sciences. 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Michael</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a387t-dee002120531ef06f3436923313aa6f0cdd35975399d5d08fa06ac9a9a6aa8f33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Analysis</topic><topic>Bacteria</topic><topic>Bacteriological methods and techniques used in bacteriology</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Cell Membrane Permeability</topic><topic>Cells</topic><topic>Electrophoresis - methods</topic><topic>Escherichia coli - cytology</topic><topic>Escherichia coli - genetics</topic><topic>Escherichia coli - metabolism</topic><topic>Flow Cytometry - instrumentation</topic><topic>Flow Cytometry - methods</topic><topic>Fluorescent Antibody Technique - methods</topic><topic>Fluorescent Dyes - metabolism</topic><topic>Fundamental and applied biological sciences. 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Michael</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Flow Cytometry of Escherichia coli on Microfluidic Devices</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. Chem</addtitle><date>2001-11-01</date><risdate>2001</risdate><volume>73</volume><issue>21</issue><spage>5334</spage><epage>5338</epage><pages>5334-5338</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><coden>ANCHAM</coden><abstract>Flow cytometry of the bacterium Escherichia coli was demonstrated on a microfabricated fluidic device (microchip). The channels were coated with poly(dimethylacrylamide) to prevent cell adhesion, and the cells were transported electrophoretically by applying potentials to the fluid reservoirs. The cells were electrophoretically focused at the channel cross and detected by coincident light scattering and fluorescence. 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| subjects | Analysis Bacteria Bacteriological methods and techniques used in bacteriology Bacteriology Biological and medical sciences Cell Membrane Permeability Cells Electrophoresis - methods Escherichia coli - cytology Escherichia coli - genetics Escherichia coli - metabolism Flow Cytometry - instrumentation Flow Cytometry - methods Fluorescent Antibody Technique - methods Fluorescent Dyes - metabolism Fundamental and applied biological sciences. Psychology Membranes Microbiology Osmosis |
| title | Flow Cytometry of Escherichia coli on Microfluidic Devices |
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