Sperm decondensation during fertilisation in the mouse: presence of DNase I hypersensitive sites in situ and a putative role for topoisomerase II
In this study our aim was to characterise the presence and the role of DNA alterations during sperm decondensation in the mouse. To visualise the changes during decondensation we investigated for the presence of DNase I hypersensitive sites in situ and for a putative role for topoisomerase II by exa...
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| Veröffentlicht in: | Zygote (Cambridge) 2000-08, Vol.8 (3), p.197-202 |
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| creator | Bizzaro, Davide Manicardi, Giancarlo Bianchi, Patrizia Grace Sakkas, Denny |
| description | In this study our aim was to characterise the presence and the role of DNA alterations during sperm decondensation in the mouse. To visualise the changes during decondensation we investigated for the presence of DNase I hypersensitive sites in situ and for a putative role for topoisomerase II by examining the effect of teniposide, a topoisomerase II inhibitor, during fertilisation. In situ nick translation without the previous addition of DNase I failed to reveal the presence of endogenous nicks in decondensing sperm and pronuclei whereas preincubation of fixed oocytes with DNase I indicated that decondensing sperm were sensitive to this enzyme. Addition of 100 μM teniposide did not completely inhibit pronuclei formation but its addition to the fertilisation medium did lead to the presence of endogenous DNA nicks in decondensing sperm. These observations suggest that DNase I hypersensitivity during sperm decondensation is related to the dramatic conformational changes that the chromatin undergoes during the decondensation process, in which topoisomerase II may be implicated. |
| doi_str_mv | 10.1017/S0967199400000988 |
| format | Article |
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These observations suggest that DNase I hypersensitivity during sperm decondensation is related to the dramatic conformational changes that the chromatin undergoes during the decondensation process, in which topoisomerase II may be implicated.</description><identifier>ISSN: 0967-1994</identifier><identifier>EISSN: 1469-8730</identifier><identifier>DOI: 10.1017/S0967199400000988</identifier><identifier>PMID: 11014498</identifier><language>eng</language><publisher>England: Cambridge University Press</publisher><subject>Animals ; Chromatin - physiology ; Deoxyribonuclease I - metabolism ; DNA Topoisomerases, Type II - metabolism ; DNase I ; Female ; Fertilisation ; Fertilization - physiology ; In situ nick translation ; In Situ Nick-End Labeling ; Male ; Mice ; Sperm decondensation ; Spermatozoa - physiology ; Teniposide - pharmacology ; Topoisomerase II</subject><ispartof>Zygote (Cambridge), 2000-08, Vol.8 (3), p.197-202</ispartof><rights>2000 Cambridge University Press</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c379t-e88ffd42e3c0bbfc854b6d2a1d9273eac926d268d82bcb38e4d8af11b8a9f3403</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.cambridge.org/core/product/identifier/S0967199400000988/type/journal_article$$EHTML$$P50$$Gcambridge$$H</linktohtml><link.rule.ids>164,315,781,785,27925,27926,55629</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11014498$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bizzaro, Davide</creatorcontrib><creatorcontrib>Manicardi, Giancarlo</creatorcontrib><creatorcontrib>Bianchi, Patrizia Grace</creatorcontrib><creatorcontrib>Sakkas, Denny</creatorcontrib><title>Sperm decondensation during fertilisation in the mouse: presence of DNase I hypersensitive sites in situ and a putative role for topoisomerase II</title><title>Zygote (Cambridge)</title><addtitle>Zygote</addtitle><description>In this study our aim was to characterise the presence and the role of DNA alterations during sperm decondensation in the mouse. 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These observations suggest that DNase I hypersensitivity during sperm decondensation is related to the dramatic conformational changes that the chromatin undergoes during the decondensation process, in which topoisomerase II may be implicated.</description><subject>Animals</subject><subject>Chromatin - physiology</subject><subject>Deoxyribonuclease I - metabolism</subject><subject>DNA Topoisomerases, Type II - metabolism</subject><subject>DNase I</subject><subject>Female</subject><subject>Fertilisation</subject><subject>Fertilization - physiology</subject><subject>In situ nick translation</subject><subject>In Situ Nick-End Labeling</subject><subject>Male</subject><subject>Mice</subject><subject>Sperm decondensation</subject><subject>Spermatozoa - physiology</subject><subject>Teniposide - pharmacology</subject><subject>Topoisomerase II</subject><issn>0967-1994</issn><issn>1469-8730</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9Uc1u1DAQthCILoUH4IJ84pYSx9nY5oZaKFstINRythx73LokdrAd1D4Gb4zTjeCAxFxmPN-PxjMIvST1CakJe3NZi44RIdp6CcH5I7QhbScqzmj9GG0WuFrwI_QspdvCYUy0T9ERKfK2FXyDfl1OEEdsQAdvwCeVXfDYzNH5a2whZje4tek8zjeAxzAneIunCAm8BhwsPvusEuAdvrkvZqWbXHY_AZcEaZGVYsbKG6zwNGf1AMYwALYh4hym4FIYIT6Y7J6jJ1YNCV6s-Rh9-_D-6vRjtf9yvjt9t680ZSJXwLm1pm2A6rrvrebbtu9Mo4gRDaOgtGjKs-OGN73uKYfWcGUJ6bkSlrY1PUavD75TDD9mSFmOLmkYBuWhfFGyhtaUCFqI5EDUMaQUwcopulHFe0lqudxB_nOHonm1ms_9COavYl18IVQHgksZ7v7gKn6XHaNsK7vzr_KKbj9diP2ZvCh8ug6hxj46cw3yNszRlw39Z4zfcQKk2g</recordid><startdate>20000801</startdate><enddate>20000801</enddate><creator>Bizzaro, Davide</creator><creator>Manicardi, Giancarlo</creator><creator>Bianchi, Patrizia Grace</creator><creator>Sakkas, Denny</creator><general>Cambridge University Press</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20000801</creationdate><title>Sperm decondensation during fertilisation in the mouse: presence of DNase I hypersensitive sites in situ and a putative role for topoisomerase II</title><author>Bizzaro, Davide ; Manicardi, Giancarlo ; Bianchi, Patrizia Grace ; Sakkas, Denny</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c379t-e88ffd42e3c0bbfc854b6d2a1d9273eac926d268d82bcb38e4d8af11b8a9f3403</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>Chromatin - physiology</topic><topic>Deoxyribonuclease I - metabolism</topic><topic>DNA Topoisomerases, Type II - metabolism</topic><topic>DNase I</topic><topic>Female</topic><topic>Fertilisation</topic><topic>Fertilization - physiology</topic><topic>In situ nick translation</topic><topic>In Situ Nick-End Labeling</topic><topic>Male</topic><topic>Mice</topic><topic>Sperm decondensation</topic><topic>Spermatozoa - physiology</topic><topic>Teniposide - pharmacology</topic><topic>Topoisomerase II</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bizzaro, Davide</creatorcontrib><creatorcontrib>Manicardi, Giancarlo</creatorcontrib><creatorcontrib>Bianchi, Patrizia Grace</creatorcontrib><creatorcontrib>Sakkas, Denny</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Zygote (Cambridge)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bizzaro, Davide</au><au>Manicardi, Giancarlo</au><au>Bianchi, Patrizia Grace</au><au>Sakkas, Denny</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sperm decondensation during fertilisation in the mouse: presence of DNase I hypersensitive sites in situ and a putative role for topoisomerase II</atitle><jtitle>Zygote (Cambridge)</jtitle><addtitle>Zygote</addtitle><date>2000-08-01</date><risdate>2000</risdate><volume>8</volume><issue>3</issue><spage>197</spage><epage>202</epage><pages>197-202</pages><issn>0967-1994</issn><eissn>1469-8730</eissn><abstract>In this study our aim was to characterise the presence and the role of DNA alterations during sperm decondensation in the mouse. To visualise the changes during decondensation we investigated for the presence of DNase I hypersensitive sites in situ and for a putative role for topoisomerase II by examining the effect of teniposide, a topoisomerase II inhibitor, during fertilisation. In situ nick translation without the previous addition of DNase I failed to reveal the presence of endogenous nicks in decondensing sperm and pronuclei whereas preincubation of fixed oocytes with DNase I indicated that decondensing sperm were sensitive to this enzyme. Addition of 100 μM teniposide did not completely inhibit pronuclei formation but its addition to the fertilisation medium did lead to the presence of endogenous DNA nicks in decondensing sperm. These observations suggest that DNase I hypersensitivity during sperm decondensation is related to the dramatic conformational changes that the chromatin undergoes during the decondensation process, in which topoisomerase II may be implicated.</abstract><cop>England</cop><pub>Cambridge University Press</pub><pmid>11014498</pmid><doi>10.1017/S0967199400000988</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0967-1994 |
| ispartof | Zygote (Cambridge), 2000-08, Vol.8 (3), p.197-202 |
| issn | 0967-1994 1469-8730 |
| language | eng |
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| source | MEDLINE; Cambridge University Press Journals Complete |
| subjects | Animals Chromatin - physiology Deoxyribonuclease I - metabolism DNA Topoisomerases, Type II - metabolism DNase I Female Fertilisation Fertilization - physiology In situ nick translation In Situ Nick-End Labeling Male Mice Sperm decondensation Spermatozoa - physiology Teniposide - pharmacology Topoisomerase II |
| title | Sperm decondensation during fertilisation in the mouse: presence of DNase I hypersensitive sites in situ and a putative role for topoisomerase II |
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