Ex vivo canine lens capsular sac explants
Lens capsular sac explants from human cadaver eyes were used to investigate posterior capsular opacification (PCO). The purpose of this study was to characterize a similar model using canine tissue and to determine whether transferrin (Tf), transforming growth factor beta-2 (TGF-beta2), and insulin-...
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| Veröffentlicht in: | Graefe's archive for clinical and experimental ophthalmology 2000-08, Vol.238 (8), p.708-714 |
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| creator | DAVIDSON, M. G WORMSTONE, M MORGAN, D MALAKOF, R ALLEN, J MCGAHAN, M. C |
| description | Lens capsular sac explants from human cadaver eyes were used to investigate posterior capsular opacification (PCO). The purpose of this study was to characterize a similar model using canine tissue and to determine whether transferrin (Tf), transforming growth factor beta-2 (TGF-beta2), and insulin-like growth factor-1 (IGF-1) are secreted by lens epithelial cells (LEC) of these ex vivo sacs.
The lens from canine eyes was removed by extracapsular cataract extraction, the lens sac dissected free, pinned to a petri dish, and cultured in either serum-supplemented or serum-free medium. Morphologic characteristics and growth rate to confluence on the posterior capsule were studied by phase-contrast microscopy. Vimentin, alpha smooth muscle actin, and panTGF-beta expression by LEC were determined by immunohistochemistry. Tf, TGF-beta2, and IGF-1 levels were measured by ELISA in the supernatant of sacs cultured in serum-free medium.
The mean time to confluence of LEC onto the posterior capsule was 5.4+/-1.1 days (n=22) and 14.7+/-3.7 days (n=14) for sacs in serum-supplemented and serum-free medium, respectively. Following development of confluence, explants displayed opacification and light scatter from cellular proliferation and capsular contraction. Confluent LEC expressed vimentin, alpha smooth muscle actin, and TGF-beta2, and both Tf and TGF-beta2 were secreted into the culture supernatant.
Canine lens sac explants have characteristics virtually identical to those of human origin, and appear to be a useful alternative tissue source for this model when human cadaver eyes are unavailable. Tf and TGFbeta-2, but not IGF-1, are secreted by LEC in explanted lens sacs and may influence the proliferation and metaplasia of LEC during the development of PCO. |
| doi_str_mv | 10.1007/s004170000158 |
| format | Article |
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The lens from canine eyes was removed by extracapsular cataract extraction, the lens sac dissected free, pinned to a petri dish, and cultured in either serum-supplemented or serum-free medium. Morphologic characteristics and growth rate to confluence on the posterior capsule were studied by phase-contrast microscopy. Vimentin, alpha smooth muscle actin, and panTGF-beta expression by LEC were determined by immunohistochemistry. Tf, TGF-beta2, and IGF-1 levels were measured by ELISA in the supernatant of sacs cultured in serum-free medium.
The mean time to confluence of LEC onto the posterior capsule was 5.4+/-1.1 days (n=22) and 14.7+/-3.7 days (n=14) for sacs in serum-supplemented and serum-free medium, respectively. Following development of confluence, explants displayed opacification and light scatter from cellular proliferation and capsular contraction. Confluent LEC expressed vimentin, alpha smooth muscle actin, and TGF-beta2, and both Tf and TGF-beta2 were secreted into the culture supernatant.
Canine lens sac explants have characteristics virtually identical to those of human origin, and appear to be a useful alternative tissue source for this model when human cadaver eyes are unavailable. Tf and TGFbeta-2, but not IGF-1, are secreted by LEC in explanted lens sacs and may influence the proliferation and metaplasia of LEC during the development of PCO.</description><identifier>ISSN: 0721-832X</identifier><identifier>EISSN: 1435-702X</identifier><identifier>DOI: 10.1007/s004170000158</identifier><identifier>PMID: 11011693</identifier><identifier>CODEN: GACODL</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Actins - metabolism ; Animals ; Biological and medical sciences ; Biomarkers ; Cataract - metabolism ; Cataract - pathology ; Cell Division ; Cells, Cultured ; Dogs ; Enzyme-Linked Immunosorbent Assay ; Insulin-Like Growth Factor I - metabolism ; Lens Capsule, Crystalline - cytology ; Lens Capsule, Crystalline - growth & development ; Lens Capsule, Crystalline - metabolism ; Medical sciences ; Ophthalmology ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Surgery of the eye and orbit ; Transferrin - metabolism ; Transforming Growth Factor beta - metabolism ; Transforming Growth Factor beta2 ; Vimentin - metabolism</subject><ispartof>Graefe's archive for clinical and experimental ophthalmology, 2000-08, Vol.238 (8), p.708-714</ispartof><rights>2000 INIST-CNRS</rights><rights>Springer-Verlag Berlin Heidelberg 2000</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c345t-48d6ff3502e3c43d3ceae8c53225a6d65a6b383f97c6a9d32d2e36bb706682673</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,27929,27930</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1469849$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11011693$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>DAVIDSON, M. G</creatorcontrib><creatorcontrib>WORMSTONE, M</creatorcontrib><creatorcontrib>MORGAN, D</creatorcontrib><creatorcontrib>MALAKOF, R</creatorcontrib><creatorcontrib>ALLEN, J</creatorcontrib><creatorcontrib>MCGAHAN, M. C</creatorcontrib><title>Ex vivo canine lens capsular sac explants</title><title>Graefe's archive for clinical and experimental ophthalmology</title><addtitle>Graefes Arch Clin Exp Ophthalmol</addtitle><description>Lens capsular sac explants from human cadaver eyes were used to investigate posterior capsular opacification (PCO). The purpose of this study was to characterize a similar model using canine tissue and to determine whether transferrin (Tf), transforming growth factor beta-2 (TGF-beta2), and insulin-like growth factor-1 (IGF-1) are secreted by lens epithelial cells (LEC) of these ex vivo sacs.
The lens from canine eyes was removed by extracapsular cataract extraction, the lens sac dissected free, pinned to a petri dish, and cultured in either serum-supplemented or serum-free medium. Morphologic characteristics and growth rate to confluence on the posterior capsule were studied by phase-contrast microscopy. Vimentin, alpha smooth muscle actin, and panTGF-beta expression by LEC were determined by immunohistochemistry. Tf, TGF-beta2, and IGF-1 levels were measured by ELISA in the supernatant of sacs cultured in serum-free medium.
The mean time to confluence of LEC onto the posterior capsule was 5.4+/-1.1 days (n=22) and 14.7+/-3.7 days (n=14) for sacs in serum-supplemented and serum-free medium, respectively. Following development of confluence, explants displayed opacification and light scatter from cellular proliferation and capsular contraction. Confluent LEC expressed vimentin, alpha smooth muscle actin, and TGF-beta2, and both Tf and TGF-beta2 were secreted into the culture supernatant.
Canine lens sac explants have characteristics virtually identical to those of human origin, and appear to be a useful alternative tissue source for this model when human cadaver eyes are unavailable. Tf and TGFbeta-2, but not IGF-1, are secreted by LEC in explanted lens sacs and may influence the proliferation and metaplasia of LEC during the development of PCO.</description><subject>Actins - metabolism</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biomarkers</subject><subject>Cataract - metabolism</subject><subject>Cataract - pathology</subject><subject>Cell Division</subject><subject>Cells, Cultured</subject><subject>Dogs</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Insulin-Like Growth Factor I - metabolism</subject><subject>Lens Capsule, Crystalline - cytology</subject><subject>Lens Capsule, Crystalline - growth & development</subject><subject>Lens Capsule, Crystalline - metabolism</subject><subject>Medical sciences</subject><subject>Ophthalmology</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>Surgery of the eye and orbit</subject><subject>Transferrin - metabolism</subject><subject>Transforming Growth Factor beta - metabolism</subject><subject>Transforming Growth Factor beta2</subject><subject>Vimentin - metabolism</subject><issn>0721-832X</issn><issn>1435-702X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNpd0M1LwzAYBvAgipvTo1cpIoKHapI3TdKjjPkBAy8Ku5U0TaGjX-Zdx_zvzVhhaA7JC_nx8PIQcs3oI6NUPSGlgikaDkv0CZkyAUmsKF-dkilVnMUa-GpCLhDXwYRPdk4mjFHGZApT8rDYRdtq20XWtFXrotq1GOYeh9r4CI2N3K6vTbvBS3JWmhrd1fjOyNfL4nP-Fi8_Xt_nz8vYgkg2sdCFLEtIKHdgBRRgnXHaJsB5YmQhw5WDhjJVVpq0AF4EKPNcUSk1lwpm5P6Q2_vue3C4yZoKravDEq4bMFMcKFNKBnj7D667wbdhtywQpbWAfVp8QNZ3iN6VWe-rxvifjNFsX2D2p8Dgb8bQIW9ccdRjYwHcjcCgNXXpTWsrPDohUy1S-AVx5HTh</recordid><startdate>20000801</startdate><enddate>20000801</enddate><creator>DAVIDSON, M. 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G ; WORMSTONE, M ; MORGAN, D ; MALAKOF, R ; ALLEN, J ; MCGAHAN, M. C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c345t-48d6ff3502e3c43d3ceae8c53225a6d65a6b383f97c6a9d32d2e36bb706682673</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Actins - metabolism</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biomarkers</topic><topic>Cataract - metabolism</topic><topic>Cataract - pathology</topic><topic>Cell Division</topic><topic>Cells, Cultured</topic><topic>Dogs</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Insulin-Like Growth Factor I - metabolism</topic><topic>Lens Capsule, Crystalline - cytology</topic><topic>Lens Capsule, Crystalline - growth & development</topic><topic>Lens Capsule, Crystalline - metabolism</topic><topic>Medical sciences</topic><topic>Ophthalmology</topic><topic>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</topic><topic>Surgery of the eye and orbit</topic><topic>Transferrin - metabolism</topic><topic>Transforming Growth Factor beta - metabolism</topic><topic>Transforming Growth Factor beta2</topic><topic>Vimentin - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>DAVIDSON, M. G</creatorcontrib><creatorcontrib>WORMSTONE, M</creatorcontrib><creatorcontrib>MORGAN, D</creatorcontrib><creatorcontrib>MALAKOF, R</creatorcontrib><creatorcontrib>ALLEN, J</creatorcontrib><creatorcontrib>MCGAHAN, M. 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G</au><au>WORMSTONE, M</au><au>MORGAN, D</au><au>MALAKOF, R</au><au>ALLEN, J</au><au>MCGAHAN, M. C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ex vivo canine lens capsular sac explants</atitle><jtitle>Graefe's archive for clinical and experimental ophthalmology</jtitle><addtitle>Graefes Arch Clin Exp Ophthalmol</addtitle><date>2000-08-01</date><risdate>2000</risdate><volume>238</volume><issue>8</issue><spage>708</spage><epage>714</epage><pages>708-714</pages><issn>0721-832X</issn><eissn>1435-702X</eissn><coden>GACODL</coden><abstract>Lens capsular sac explants from human cadaver eyes were used to investigate posterior capsular opacification (PCO). The purpose of this study was to characterize a similar model using canine tissue and to determine whether transferrin (Tf), transforming growth factor beta-2 (TGF-beta2), and insulin-like growth factor-1 (IGF-1) are secreted by lens epithelial cells (LEC) of these ex vivo sacs.
The lens from canine eyes was removed by extracapsular cataract extraction, the lens sac dissected free, pinned to a petri dish, and cultured in either serum-supplemented or serum-free medium. Morphologic characteristics and growth rate to confluence on the posterior capsule were studied by phase-contrast microscopy. Vimentin, alpha smooth muscle actin, and panTGF-beta expression by LEC were determined by immunohistochemistry. Tf, TGF-beta2, and IGF-1 levels were measured by ELISA in the supernatant of sacs cultured in serum-free medium.
The mean time to confluence of LEC onto the posterior capsule was 5.4+/-1.1 days (n=22) and 14.7+/-3.7 days (n=14) for sacs in serum-supplemented and serum-free medium, respectively. Following development of confluence, explants displayed opacification and light scatter from cellular proliferation and capsular contraction. Confluent LEC expressed vimentin, alpha smooth muscle actin, and TGF-beta2, and both Tf and TGF-beta2 were secreted into the culture supernatant.
Canine lens sac explants have characteristics virtually identical to those of human origin, and appear to be a useful alternative tissue source for this model when human cadaver eyes are unavailable. Tf and TGFbeta-2, but not IGF-1, are secreted by LEC in explanted lens sacs and may influence the proliferation and metaplasia of LEC during the development of PCO.</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>11011693</pmid><doi>10.1007/s004170000158</doi><tpages>7</tpages></addata></record> |
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| language | eng |
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| source | MEDLINE; SpringerNature Journals |
| subjects | Actins - metabolism Animals Biological and medical sciences Biomarkers Cataract - metabolism Cataract - pathology Cell Division Cells, Cultured Dogs Enzyme-Linked Immunosorbent Assay Insulin-Like Growth Factor I - metabolism Lens Capsule, Crystalline - cytology Lens Capsule, Crystalline - growth & development Lens Capsule, Crystalline - metabolism Medical sciences Ophthalmology Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Surgery of the eye and orbit Transferrin - metabolism Transforming Growth Factor beta - metabolism Transforming Growth Factor beta2 Vimentin - metabolism |
| title | Ex vivo canine lens capsular sac explants |
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