A dual electrospray ionization source combined with hexapole accumulation to achieve high mass accuracy of biopolymers in fourier transform ion cyclotron resonance mass spectrometry
A dual electrospray ionization (ESI) source employed with hexapole accumulation and gated trapping provides a novel method of using an internal standard to achieve high mass accuracies in Fourier transform ion cyclotron resonance mass spectrometry. Two ESI emitters are sequentially positioned in fro...
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| Veröffentlicht in: | Journal of the American Society for Mass Spectrometry 2000-10, Vol.11 (10), p.876-883 |
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| creator | Hannis, James C Muddiman, David C |
| description | A dual electrospray ionization (ESI) source employed with hexapole accumulation and gated trapping provides a novel method of using an internal standard to achieve high mass accuracies in Fourier transform ion cyclotron resonance mass spectrometry. Two ESI emitters are sequentially positioned in front of the heated metal capillary inlet by a solenoid fitted to an XYZ micromanipulator; one emitter contains the analyte(s) of interest and the other an internal standard. A 5 V transistor–transistor logic pulse from the data station controls the solenoid by means of a solid-state relay so that matching of spectral peak intensities (i.e., analyte and internal standard intensities) can be accomplished by adjusting the hexapole accumulation time for each species. Polythymidine, d(pT)
18, was used as the internal standard for all studies reported here. The absolute average error for an internally calibrated 15-mer oligonucleotide (theoretical monoisotopic mass = 4548.769 Da) was −1.1 ppm (external calibration: 41 ppm) with a standard deviation of ±3.0 ppm (external calibration: ±24 ppm) for a total of 25 spectra obtained at various hexapole accumulation time ratios. Linear least squares regression analysis was carried out and revealed a linear dependence of the magnitudes of the peak height ratios (analyte/internal standard) vs. hexapole accumulation time ratios (analyte/internal standard) which is described by the following equation:
y = 0.45
x −0.02. The fitted line had a %RSD of the slope of 28% with an
R
2 of 0.93. The applicability of this methodology was extended to a polymerase chain reaction product with a theoretical average molecular mass of 50,849.20 Da. With the internal standard, d(pT)
18, an absolute average error of −8.9 ppm (external calibration: 44 ppm) based on five measurements was achieved with a standard deviation of 11 ppm (external calibration: ±36 ppm), thus illustrating this method’s use for characterizing large biomolecules such as those encountered in genomics and proteomics related research. |
| doi_str_mv | 10.1016/S1044-0305(00)00160-4 |
| format | Article |
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18, was used as the internal standard for all studies reported here. The absolute average error for an internally calibrated 15-mer oligonucleotide (theoretical monoisotopic mass = 4548.769 Da) was −1.1 ppm (external calibration: 41 ppm) with a standard deviation of ±3.0 ppm (external calibration: ±24 ppm) for a total of 25 spectra obtained at various hexapole accumulation time ratios. Linear least squares regression analysis was carried out and revealed a linear dependence of the magnitudes of the peak height ratios (analyte/internal standard) vs. hexapole accumulation time ratios (analyte/internal standard) which is described by the following equation:
y = 0.45
x −0.02. The fitted line had a %RSD of the slope of 28% with an
R
2 of 0.93. The applicability of this methodology was extended to a polymerase chain reaction product with a theoretical average molecular mass of 50,849.20 Da. With the internal standard, d(pT)
18, an absolute average error of −8.9 ppm (external calibration: 44 ppm) based on five measurements was achieved with a standard deviation of 11 ppm (external calibration: ±36 ppm), thus illustrating this method’s use for characterizing large biomolecules such as those encountered in genomics and proteomics related research.</description><identifier>ISSN: 1044-0305</identifier><identifier>EISSN: 1879-1123</identifier><identifier>DOI: 10.1016/S1044-0305(00)00160-4</identifier><identifier>PMID: 11014449</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Accumulation ; Biological and medical sciences ; Biomolecules ; Biopolymers ; Biopolymers - chemistry ; Calibration ; Cyclotron resonance ; Cyclotrons ; Diverse techniques ; Electrospraying ; Emitters ; Fourier Analysis ; Fourier transforms ; Fundamental and applied biological sciences. Psychology ; Ionization ; Ions ; Mass Spectrometry ; Molecular and cellular biology ; Polymerase chain reaction ; Proteomics ; Regression analysis ; Reverse Transcriptase Polymerase Chain Reaction ; Scientific imaging ; Spectrophotometry, Ultraviolet ; Spectroscopy ; Standard deviation ; Transistor logic ; Transistors</subject><ispartof>Journal of the American Society for Mass Spectrometry, 2000-10, Vol.11 (10), p.876-883</ispartof><rights>2000 American Society for Mass Spectrometry</rights><rights>2000 INIST-CNRS</rights><rights>American Society for Mass Spectrometry 2000</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c418t-e178c276e3aabdf7862807f41a838c422ede82d02351033593eff0c422d462f93</citedby><cites>FETCH-LOGICAL-c418t-e178c276e3aabdf7862807f41a838c422ede82d02351033593eff0c422d462f93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1508725$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11014449$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hannis, James C</creatorcontrib><creatorcontrib>Muddiman, David C</creatorcontrib><title>A dual electrospray ionization source combined with hexapole accumulation to achieve high mass accuracy of biopolymers in fourier transform ion cyclotron resonance mass spectrometry</title><title>Journal of the American Society for Mass Spectrometry</title><addtitle>J Am Soc Mass Spectrom</addtitle><description>A dual electrospray ionization (ESI) source employed with hexapole accumulation and gated trapping provides a novel method of using an internal standard to achieve high mass accuracies in Fourier transform ion cyclotron resonance mass spectrometry. Two ESI emitters are sequentially positioned in front of the heated metal capillary inlet by a solenoid fitted to an XYZ micromanipulator; one emitter contains the analyte(s) of interest and the other an internal standard. A 5 V transistor–transistor logic pulse from the data station controls the solenoid by means of a solid-state relay so that matching of spectral peak intensities (i.e., analyte and internal standard intensities) can be accomplished by adjusting the hexapole accumulation time for each species. Polythymidine, d(pT)
18, was used as the internal standard for all studies reported here. The absolute average error for an internally calibrated 15-mer oligonucleotide (theoretical monoisotopic mass = 4548.769 Da) was −1.1 ppm (external calibration: 41 ppm) with a standard deviation of ±3.0 ppm (external calibration: ±24 ppm) for a total of 25 spectra obtained at various hexapole accumulation time ratios. Linear least squares regression analysis was carried out and revealed a linear dependence of the magnitudes of the peak height ratios (analyte/internal standard) vs. hexapole accumulation time ratios (analyte/internal standard) which is described by the following equation:
y = 0.45
x −0.02. The fitted line had a %RSD of the slope of 28% with an
R
2 of 0.93. The applicability of this methodology was extended to a polymerase chain reaction product with a theoretical average molecular mass of 50,849.20 Da. With the internal standard, d(pT)
18, an absolute average error of −8.9 ppm (external calibration: 44 ppm) based on five measurements was achieved with a standard deviation of 11 ppm (external calibration: ±36 ppm), thus illustrating this method’s use for characterizing large biomolecules such as those encountered in genomics and proteomics related research.</description><subject>Accumulation</subject><subject>Biological and medical sciences</subject><subject>Biomolecules</subject><subject>Biopolymers</subject><subject>Biopolymers - chemistry</subject><subject>Calibration</subject><subject>Cyclotron resonance</subject><subject>Cyclotrons</subject><subject>Diverse techniques</subject><subject>Electrospraying</subject><subject>Emitters</subject><subject>Fourier Analysis</subject><subject>Fourier transforms</subject><subject>Fundamental and applied biological sciences. 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Psychology</topic><topic>Ionization</topic><topic>Ions</topic><topic>Mass Spectrometry</topic><topic>Molecular and cellular biology</topic><topic>Polymerase chain reaction</topic><topic>Proteomics</topic><topic>Regression analysis</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Scientific imaging</topic><topic>Spectrophotometry, Ultraviolet</topic><topic>Spectroscopy</topic><topic>Standard deviation</topic><topic>Transistor logic</topic><topic>Transistors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hannis, James C</creatorcontrib><creatorcontrib>Muddiman, David C</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of the American Society for Mass Spectrometry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hannis, James C</au><au>Muddiman, David C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A dual electrospray ionization source combined with hexapole accumulation to achieve high mass accuracy of biopolymers in fourier transform ion cyclotron resonance mass spectrometry</atitle><jtitle>Journal of the American Society for Mass Spectrometry</jtitle><addtitle>J Am Soc Mass Spectrom</addtitle><date>2000-10-01</date><risdate>2000</risdate><volume>11</volume><issue>10</issue><spage>876</spage><epage>883</epage><pages>876-883</pages><issn>1044-0305</issn><eissn>1879-1123</eissn><abstract>A dual electrospray ionization (ESI) source employed with hexapole accumulation and gated trapping provides a novel method of using an internal standard to achieve high mass accuracies in Fourier transform ion cyclotron resonance mass spectrometry. Two ESI emitters are sequentially positioned in front of the heated metal capillary inlet by a solenoid fitted to an XYZ micromanipulator; one emitter contains the analyte(s) of interest and the other an internal standard. A 5 V transistor–transistor logic pulse from the data station controls the solenoid by means of a solid-state relay so that matching of spectral peak intensities (i.e., analyte and internal standard intensities) can be accomplished by adjusting the hexapole accumulation time for each species. Polythymidine, d(pT)
18, was used as the internal standard for all studies reported here. The absolute average error for an internally calibrated 15-mer oligonucleotide (theoretical monoisotopic mass = 4548.769 Da) was −1.1 ppm (external calibration: 41 ppm) with a standard deviation of ±3.0 ppm (external calibration: ±24 ppm) for a total of 25 spectra obtained at various hexapole accumulation time ratios. Linear least squares regression analysis was carried out and revealed a linear dependence of the magnitudes of the peak height ratios (analyte/internal standard) vs. hexapole accumulation time ratios (analyte/internal standard) which is described by the following equation:
y = 0.45
x −0.02. The fitted line had a %RSD of the slope of 28% with an
R
2 of 0.93. The applicability of this methodology was extended to a polymerase chain reaction product with a theoretical average molecular mass of 50,849.20 Da. With the internal standard, d(pT)
18, an absolute average error of −8.9 ppm (external calibration: 44 ppm) based on five measurements was achieved with a standard deviation of 11 ppm (external calibration: ±36 ppm), thus illustrating this method’s use for characterizing large biomolecules such as those encountered in genomics and proteomics related research.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>11014449</pmid><doi>10.1016/S1044-0305(00)00160-4</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Accumulation Biological and medical sciences Biomolecules Biopolymers Biopolymers - chemistry Calibration Cyclotron resonance Cyclotrons Diverse techniques Electrospraying Emitters Fourier Analysis Fourier transforms Fundamental and applied biological sciences. Psychology Ionization Ions Mass Spectrometry Molecular and cellular biology Polymerase chain reaction Proteomics Regression analysis Reverse Transcriptase Polymerase Chain Reaction Scientific imaging Spectrophotometry, Ultraviolet Spectroscopy Standard deviation Transistor logic Transistors |
| title | A dual electrospray ionization source combined with hexapole accumulation to achieve high mass accuracy of biopolymers in fourier transform ion cyclotron resonance mass spectrometry |
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