The bacteriophage lambda DNA packaging enzyme: identification of four structural domains of the gpNu1 subunit using limited proteolysis

Lambda DNA terminase, the enzyme that cleaves virion-length chromosomes from multigenomic concatemers and packages them into the bacteriophage head, is composed of two subunits, gpNu1 and gpA. Direct determination of the structure of gpNu1, the smaller subunit, has not been possible because of its i...

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Veröffentlicht in:	Biological research 2001, Vol.34 (3-4), p.207-216
Hauptverfasser:	Araya, P, Rosemblatt, M, Valenzuela, P, Murialdo, H
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Bacteriophage lambda - chemistry Bacteriophage lambda - enzymology Bacteriophage lambda - genetics Chymotrypsin - chemistry DNA, Viral - metabolism Electrophoresis, Gel, Two-Dimensional Electrophoresis, Polyacrylamide Gel Guanidine - metabolism Molecular Weight Peptide Hydrolases - metabolism Protein Folding Protein Structure, Tertiary Viral Proteins - chemistry Viral Proteins - metabolism
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