Improved RNA extraction and one-tube RT-PCR assay for simultaneous detection of control plant RNA plus several viruses in plant extracts
A procedure was developed for simultaneous detection of plant RNA viruses and of plant RNA, as a control. RT-PCR amplification with primers designed for the detection of the plant mRNAs encoding malate dehydrogenase (MDH) and the large subunit of ribulose bisphosphate carboxylase oxygenase (RubiscoL...
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| Veröffentlicht in: | Journal of virological methods 2000-10, Vol.90 (1), p.37-49 |
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| creator | Nassuth, Annette Pollari, Elisa Helmeczy, Katherine Stewart, Sandra Kofalvi, Sandra A |
| description | A procedure was developed for simultaneous detection of plant RNA viruses and of plant RNA, as a control. RT-PCR amplification with primers designed for the detection of the plant mRNAs encoding malate dehydrogenase (MDH) and the large subunit of ribulose bisphosphate carboxylase oxygenase (RubiscoL) was used for the development of a plant extraction procedure that consistently yields extracts that can be amplified. The control amplification was used successfully on extracts from cane, leaf and/or bud tissues from grapevine, apple, raspberry, strawberry, peach, apricot, plum and wheat. Multiplex RT-PCR conditions were established for the simultaneous detection in grapevine extracts of either arabis mosaic virus, rupestris stem pitting associated virus and malate dehydrogenase mRNA, or grapevine virus A, grapevine virus B, grapevine leafroll associated virus-3, and RubiscoL mRNA. |
| doi_str_mv | 10.1016/S0166-0934(00)00211-1 |
| format | Article |
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RT-PCR amplification with primers designed for the detection of the plant mRNAs encoding malate dehydrogenase (MDH) and the large subunit of ribulose bisphosphate carboxylase oxygenase (RubiscoL) was used for the development of a plant extraction procedure that consistently yields extracts that can be amplified. The control amplification was used successfully on extracts from cane, leaf and/or bud tissues from grapevine, apple, raspberry, strawberry, peach, apricot, plum and wheat. Multiplex RT-PCR conditions were established for the simultaneous detection in grapevine extracts of either arabis mosaic virus, rupestris stem pitting associated virus and malate dehydrogenase mRNA, or grapevine virus A, grapevine virus B, grapevine leafroll associated virus-3, and RubiscoL mRNA.</description><identifier>ISSN: 0166-0934</identifier><identifier>EISSN: 1879-0984</identifier><identifier>DOI: 10.1016/S0166-0934(00)00211-1</identifier><identifier>PMID: 11011079</identifier><identifier>CODEN: JVMEDH</identifier><language>eng</language><publisher>London: Elsevier B.V</publisher><subject>Arabis mosaic virus ; Biological and medical sciences ; Detection ; Fundamental and applied biological sciences. Psychology ; Generalities. Techniques. Transmission, epidemiology, ecology. Antiviral substances, control ; Grapevine leafroll associated virus 3 ; Grapevine virus A ; Grapevine virus B ; Grapevine viruses ; Malate Dehydrogenase - genetics ; Malate Dehydrogenase - metabolism ; Microbiology ; Multiplex RT-PCR ; Phytopathology. Animal pests. Plant and forest protection ; Plant Diseases - virology ; Plant Extracts - analysis ; Plant Viruses - genetics ; Plant Viruses - isolation & purification ; Plant viruses and viroids ; Reverse Transcriptase Polymerase Chain Reaction ; Ribulose-Bisphosphate Carboxylase - genetics ; Ribulose-Bisphosphate Carboxylase - metabolism ; RNA, Plant - analysis ; RNA, Viral - analysis ; RNA, Viral - isolation & purification ; Rosales - virology ; RT-PCR control ; Techniques used in virology ; Virology ; Woody plant extraction</subject><ispartof>Journal of virological methods, 2000-10, Vol.90 (1), p.37-49</ispartof><rights>2000 Elsevier Science B.V.</rights><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c420t-efe32c75b7266b59530205cebe103ddc517d242eb0cb43ecfbfddf2ea71d61a33</citedby><cites>FETCH-LOGICAL-c420t-efe32c75b7266b59530205cebe103ddc517d242eb0cb43ecfbfddf2ea71d61a33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0166-0934(00)00211-1$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=925325$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11011079$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nassuth, Annette</creatorcontrib><creatorcontrib>Pollari, Elisa</creatorcontrib><creatorcontrib>Helmeczy, Katherine</creatorcontrib><creatorcontrib>Stewart, Sandra</creatorcontrib><creatorcontrib>Kofalvi, Sandra A</creatorcontrib><title>Improved RNA extraction and one-tube RT-PCR assay for simultaneous detection of control plant RNA plus several viruses in plant extracts</title><title>Journal of virological methods</title><addtitle>J Virol Methods</addtitle><description>A procedure was developed for simultaneous detection of plant RNA viruses and of plant RNA, as a control. RT-PCR amplification with primers designed for the detection of the plant mRNAs encoding malate dehydrogenase (MDH) and the large subunit of ribulose bisphosphate carboxylase oxygenase (RubiscoL) was used for the development of a plant extraction procedure that consistently yields extracts that can be amplified. The control amplification was used successfully on extracts from cane, leaf and/or bud tissues from grapevine, apple, raspberry, strawberry, peach, apricot, plum and wheat. Multiplex RT-PCR conditions were established for the simultaneous detection in grapevine extracts of either arabis mosaic virus, rupestris stem pitting associated virus and malate dehydrogenase mRNA, or grapevine virus A, grapevine virus B, grapevine leafroll associated virus-3, and RubiscoL mRNA.</description><subject>Arabis mosaic virus</subject><subject>Biological and medical sciences</subject><subject>Detection</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Generalities. Techniques. Transmission, epidemiology, ecology. Antiviral substances, control</subject><subject>Grapevine leafroll associated virus 3</subject><subject>Grapevine virus A</subject><subject>Grapevine virus B</subject><subject>Grapevine viruses</subject><subject>Malate Dehydrogenase - genetics</subject><subject>Malate Dehydrogenase - metabolism</subject><subject>Microbiology</subject><subject>Multiplex RT-PCR</subject><subject>Phytopathology. Animal pests. Plant and forest protection</subject><subject>Plant Diseases - virology</subject><subject>Plant Extracts - analysis</subject><subject>Plant Viruses - genetics</subject><subject>Plant Viruses - isolation & purification</subject><subject>Plant viruses and viroids</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Ribulose-Bisphosphate Carboxylase - genetics</subject><subject>Ribulose-Bisphosphate Carboxylase - metabolism</subject><subject>RNA, Plant - analysis</subject><subject>RNA, Viral - analysis</subject><subject>RNA, Viral - isolation & purification</subject><subject>Rosales - virology</subject><subject>RT-PCR control</subject><subject>Techniques used in virology</subject><subject>Virology</subject><subject>Woody plant extraction</subject><issn>0166-0934</issn><issn>1879-0984</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkdFqFDEUhoModrv6CEpAEL2YepLMTHauSlmsLRQra70OmeQEIjOTNZlZ7Bv42KY7Q3tZCAmE7z8nJx8h7xicMWD1l595qwtoRPkJ4DMAZ6xgL8iKbWSTrzflS7J6RE7IaUq_AaCSQrwmJyyXYCCbFfl33e9jOKClu-8XFP-OUZvRh4HqwdIwYDFOLdLdXfFju6M6JX1PXYg0-X7qRj1gmBK1OOIcCo6aMIwxdHTf6WE8Ft13mUl4wKg7evBxSpioHxZiaZnekFdOdwnfLuea_Lr8ere9Km5uv11vL24KU3IYC3QouJFVK3ldt1VTCeBQGWyRgbDWVExaXnJswbSlQONaZ63jqCWzNdNCrMnHuW4e-8-EaVS9Twa7bh5GSc6baiPksyCTNS8bKDNYzaCJIaWITu2j73W8VwzUgyt1dKUeRCgAdXSlWM69XxpMbY_2KbXIycCHBdDJ6M5FPRifHrmGVyKvNTmfKcy_dvAYVTIeB4PWx6xF2eCfech_vyWyDA</recordid><startdate>20001001</startdate><enddate>20001001</enddate><creator>Nassuth, Annette</creator><creator>Pollari, Elisa</creator><creator>Helmeczy, Katherine</creator><creator>Stewart, Sandra</creator><creator>Kofalvi, Sandra A</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20001001</creationdate><title>Improved RNA extraction and one-tube RT-PCR assay for simultaneous detection of control plant RNA plus several viruses in plant extracts</title><author>Nassuth, Annette ; Pollari, Elisa ; Helmeczy, Katherine ; Stewart, Sandra ; Kofalvi, Sandra A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c420t-efe32c75b7266b59530205cebe103ddc517d242eb0cb43ecfbfddf2ea71d61a33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Arabis mosaic virus</topic><topic>Biological and medical sciences</topic><topic>Detection</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Generalities. Techniques. Transmission, epidemiology, ecology. Antiviral substances, control</topic><topic>Grapevine leafroll associated virus 3</topic><topic>Grapevine virus A</topic><topic>Grapevine virus B</topic><topic>Grapevine viruses</topic><topic>Malate Dehydrogenase - genetics</topic><topic>Malate Dehydrogenase - metabolism</topic><topic>Microbiology</topic><topic>Multiplex RT-PCR</topic><topic>Phytopathology. Animal pests. Plant and forest protection</topic><topic>Plant Diseases - virology</topic><topic>Plant Extracts - analysis</topic><topic>Plant Viruses - genetics</topic><topic>Plant Viruses - isolation & purification</topic><topic>Plant viruses and viroids</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Ribulose-Bisphosphate Carboxylase - genetics</topic><topic>Ribulose-Bisphosphate Carboxylase - metabolism</topic><topic>RNA, Plant - analysis</topic><topic>RNA, Viral - analysis</topic><topic>RNA, Viral - isolation & purification</topic><topic>Rosales - virology</topic><topic>RT-PCR control</topic><topic>Techniques used in virology</topic><topic>Virology</topic><topic>Woody plant extraction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nassuth, Annette</creatorcontrib><creatorcontrib>Pollari, Elisa</creatorcontrib><creatorcontrib>Helmeczy, Katherine</creatorcontrib><creatorcontrib>Stewart, Sandra</creatorcontrib><creatorcontrib>Kofalvi, Sandra A</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of virological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nassuth, Annette</au><au>Pollari, Elisa</au><au>Helmeczy, Katherine</au><au>Stewart, Sandra</au><au>Kofalvi, Sandra A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Improved RNA extraction and one-tube RT-PCR assay for simultaneous detection of control plant RNA plus several viruses in plant extracts</atitle><jtitle>Journal of virological methods</jtitle><addtitle>J Virol Methods</addtitle><date>2000-10-01</date><risdate>2000</risdate><volume>90</volume><issue>1</issue><spage>37</spage><epage>49</epage><pages>37-49</pages><issn>0166-0934</issn><eissn>1879-0984</eissn><coden>JVMEDH</coden><abstract>A procedure was developed for simultaneous detection of plant RNA viruses and of plant RNA, as a control. RT-PCR amplification with primers designed for the detection of the plant mRNAs encoding malate dehydrogenase (MDH) and the large subunit of ribulose bisphosphate carboxylase oxygenase (RubiscoL) was used for the development of a plant extraction procedure that consistently yields extracts that can be amplified. The control amplification was used successfully on extracts from cane, leaf and/or bud tissues from grapevine, apple, raspberry, strawberry, peach, apricot, plum and wheat. Multiplex RT-PCR conditions were established for the simultaneous detection in grapevine extracts of either arabis mosaic virus, rupestris stem pitting associated virus and malate dehydrogenase mRNA, or grapevine virus A, grapevine virus B, grapevine leafroll associated virus-3, and RubiscoL mRNA.</abstract><cop>London</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>11011079</pmid><doi>10.1016/S0166-0934(00)00211-1</doi><tpages>13</tpages></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Arabis mosaic virus Biological and medical sciences Detection Fundamental and applied biological sciences. Psychology Generalities. Techniques. Transmission, epidemiology, ecology. Antiviral substances, control Grapevine leafroll associated virus 3 Grapevine virus A Grapevine virus B Grapevine viruses Malate Dehydrogenase - genetics Malate Dehydrogenase - metabolism Microbiology Multiplex RT-PCR Phytopathology. Animal pests. Plant and forest protection Plant Diseases - virology Plant Extracts - analysis Plant Viruses - genetics Plant Viruses - isolation & purification Plant viruses and viroids Reverse Transcriptase Polymerase Chain Reaction Ribulose-Bisphosphate Carboxylase - genetics Ribulose-Bisphosphate Carboxylase - metabolism RNA, Plant - analysis RNA, Viral - analysis RNA, Viral - isolation & purification Rosales - virology RT-PCR control Techniques used in virology Virology Woody plant extraction |
| title | Improved RNA extraction and one-tube RT-PCR assay for simultaneous detection of control plant RNA plus several viruses in plant extracts |
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