The Effects of Melatonin on the Antioxidant Systems in Experimental Spinal Injury
Melatonin has been recently shown by various in-vivo and in-vitro studies to exert potent neutralising effects on hydroxyl radicals, stimulate glutathione peroxidase (GSH-Px) activity, and protect catalase (CAT) from the destructive activity of hydroxyl radicals in neural tissue. We aimed to investi...
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| creator | Taskiran, Dilek Tanyalcin, Then Sozmen, Eser Y. Peker, Gonul O. Gulmen, Vehbi Cagli, Sedat Kanit, Luttfiye Tekeli, Gurkan Barcin, Erol Zileli, Mehmet Kutay, Fatma Z. |
| description | Melatonin has been recently shown by various in-vivo and in-vitro studies to exert potent neutralising effects on hydroxyl radicals, stimulate glutathione peroxidase (GSH-Px) activity, and protect catalase (CAT) from the destructive activity of hydroxyl radicals in neural tissue. We aimed to investigate the possible effects of pharmacological dose of melatonin on some of the antioxidant defence systems in an in-vivo study of experimental spinal injury. Seven groups of adult male Sprague Dawley rats were used in the following scheme: Group I: Naïve (n = 6), Group II: Lesion (n = 8), Group III: Melatonin (n = 5), Group IV: Melatonin + Lesion (n = 8), Group V: Placebo + Lesion (n = 5), Group VI: Sham operation (n = 5), and Group VII: Placebo (n = 5). Experimental spinal injury was induced at level T7-T8 by 5 sec compression of the total cord with an aneurism clip on anaesthetised and laminectomized animals. The total 10mg/kg dose of melatonin (Sigma) dissolved in alcohol-water was administered i.p. four times in 2.5 mg/kg doses, at 20min pre-, at the time of and at 1h and 2h post-compression. At 24±2h post-injury, the rats were euthanized and the lesioned segments of cord were dissected and homogenised with special care taken to distribute equal amount of injured tissue in each sample for analysis of reduced glutathione (GSH), oxidised glutathione (GSSG), superoxide dismutase (SOD), and CAT activity. Compression injury decreased GSH/GSSG ratio significantly (p |
| doi_str_mv | 10.3109/00207450009035009 |
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We aimed to investigate the possible effects of pharmacological dose of melatonin on some of the antioxidant defence systems in an in-vivo study of experimental spinal injury. Seven groups of adult male Sprague Dawley rats were used in the following scheme: Group I: Naïve (n = 6), Group II: Lesion (n = 8), Group III: Melatonin (n = 5), Group IV: Melatonin + Lesion (n = 8), Group V: Placebo + Lesion (n = 5), Group VI: Sham operation (n = 5), and Group VII: Placebo (n = 5). Experimental spinal injury was induced at level T7-T8 by 5 sec compression of the total cord with an aneurism clip on anaesthetised and laminectomized animals. The total 10mg/kg dose of melatonin (Sigma) dissolved in alcohol-water was administered i.p. four times in 2.5 mg/kg doses, at 20min pre-, at the time of and at 1h and 2h post-compression. At 24±2h post-injury, the rats were euthanized and the lesioned segments of cord were dissected and homogenised with special care taken to distribute equal amount of injured tissue in each sample for analysis of reduced glutathione (GSH), oxidised glutathione (GSSG), superoxide dismutase (SOD), and CAT activity. Compression injury decreased GSH/GSSG ratio significantly (p <. 0001). Melatonin, by itself, significantly decreased GSSG content (p <. 05) and increased CAT activity (p <. 05) in the naive rats. Melatonin treatment decreased GSSG activity, thus elevating GSH/GSSG ratio, and also increased SOD and CAT activity without reaching statistical significance in the lesioned animals. In conclusion, pharmacological dose of systemically applied melatonin seemed to support some features of the antioxidant defence systems in our hands.</description><identifier>ISSN: 0020-7454</identifier><identifier>EISSN: 1563-5279</identifier><identifier>EISSN: 1543-5245</identifier><identifier>DOI: 10.3109/00207450009035009</identifier><identifier>PMID: 11011974</identifier><identifier>CODEN: IJNUB7</identifier><language>eng</language><publisher>London: Informa UK Ltd</publisher><subject>Animals ; Biological and medical sciences ; catalase (CAT) ; Catalase - metabolism ; Central nervous system ; Central neurotransmission. Neuromudulation. Pathways and receptors ; Clip compression ; Disease Models, Animal ; Fundamental and applied biological sciences. Psychology ; Glutathione - metabolism ; Humans ; Male ; melatonin ; Melatonin - administration & dosage ; Melatonin - pharmacology ; Melatonin - therapeutic use ; Neurons - drug effects ; oxidized glutathione (GSSG) ; Rats ; Rats, Sprague-Dawley ; reduced glutathione (GSH) ; Spinal Cord Injuries - drug therapy ; Spinal Cord Injuries - enzymology ; superoxide dismutase (SOD) ; Superoxide Dismutase - metabolism ; Vertebrates: nervous system and sense organs</subject><ispartof>International journal of neuroscience, 2000-01, Vol.104 (1), p.63-73</ispartof><rights>2000 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 2000</rights><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c462t-27a59ccdde9902e7a63fab3c9cdc440bb304f732ab413cdb5dde58458777bd63</citedby><cites>FETCH-LOGICAL-c462t-27a59ccdde9902e7a63fab3c9cdc440bb304f732ab413cdb5dde58458777bd63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.3109/00207450009035009$$EPDF$$P50$$Ginformaworld$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.3109/00207450009035009$$EHTML$$P50$$Ginformaworld$$H</linktohtml><link.rule.ids>315,781,785,27929,27930,59652,59758,60441,60547,61226,61261,61407,61442</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1021939$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11011974$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Taskiran, Dilek</creatorcontrib><creatorcontrib>Tanyalcin, Then</creatorcontrib><creatorcontrib>Sozmen, Eser Y.</creatorcontrib><creatorcontrib>Peker, Gonul O.</creatorcontrib><creatorcontrib>Gulmen, Vehbi</creatorcontrib><creatorcontrib>Cagli, Sedat</creatorcontrib><creatorcontrib>Kanit, Luttfiye</creatorcontrib><creatorcontrib>Tekeli, Gurkan</creatorcontrib><creatorcontrib>Barcin, Erol</creatorcontrib><creatorcontrib>Zileli, Mehmet</creatorcontrib><creatorcontrib>Kutay, Fatma Z.</creatorcontrib><title>The Effects of Melatonin on the Antioxidant Systems in Experimental Spinal Injury</title><title>International journal of neuroscience</title><addtitle>Int J Neurosci</addtitle><description>Melatonin has been recently shown by various in-vivo and in-vitro studies to exert potent neutralising effects on hydroxyl radicals, stimulate glutathione peroxidase (GSH-Px) activity, and protect catalase (CAT) from the destructive activity of hydroxyl radicals in neural tissue. We aimed to investigate the possible effects of pharmacological dose of melatonin on some of the antioxidant defence systems in an in-vivo study of experimental spinal injury. Seven groups of adult male Sprague Dawley rats were used in the following scheme: Group I: Naïve (n = 6), Group II: Lesion (n = 8), Group III: Melatonin (n = 5), Group IV: Melatonin + Lesion (n = 8), Group V: Placebo + Lesion (n = 5), Group VI: Sham operation (n = 5), and Group VII: Placebo (n = 5). Experimental spinal injury was induced at level T7-T8 by 5 sec compression of the total cord with an aneurism clip on anaesthetised and laminectomized animals. The total 10mg/kg dose of melatonin (Sigma) dissolved in alcohol-water was administered i.p. four times in 2.5 mg/kg doses, at 20min pre-, at the time of and at 1h and 2h post-compression. At 24±2h post-injury, the rats were euthanized and the lesioned segments of cord were dissected and homogenised with special care taken to distribute equal amount of injured tissue in each sample for analysis of reduced glutathione (GSH), oxidised glutathione (GSSG), superoxide dismutase (SOD), and CAT activity. Compression injury decreased GSH/GSSG ratio significantly (p <. 0001). Melatonin, by itself, significantly decreased GSSG content (p <. 05) and increased CAT activity (p <. 05) in the naive rats. Melatonin treatment decreased GSSG activity, thus elevating GSH/GSSG ratio, and also increased SOD and CAT activity without reaching statistical significance in the lesioned animals. In conclusion, pharmacological dose of systemically applied melatonin seemed to support some features of the antioxidant defence systems in our hands.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>catalase (CAT)</subject><subject>Catalase - metabolism</subject><subject>Central nervous system</subject><subject>Central neurotransmission. Neuromudulation. Pathways and receptors</subject><subject>Clip compression</subject><subject>Disease Models, Animal</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glutathione - metabolism</subject><subject>Humans</subject><subject>Male</subject><subject>melatonin</subject><subject>Melatonin - administration & dosage</subject><subject>Melatonin - pharmacology</subject><subject>Melatonin - therapeutic use</subject><subject>Neurons - drug effects</subject><subject>oxidized glutathione (GSSG)</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>reduced glutathione (GSH)</subject><subject>Spinal Cord Injuries - drug therapy</subject><subject>Spinal Cord Injuries - enzymology</subject><subject>superoxide dismutase (SOD)</subject><subject>Superoxide Dismutase - metabolism</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0020-7454</issn><issn>1563-5279</issn><issn>1543-5245</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkV9LHDEUxUNpqYv6AfpS5kH6NjX_ZjJBX0RWKyiluO_DnUzCRjLJmmSo--2bZbe0UrBPl3B-55B7LkKfCP7KCJbnGFMseIMxlpiVId-hBWlaVjdUyPdosdPrAvAjdJqSHcqbSUm77iM6IgQTIgVfoB-rta6WxmiVUxVM9aAd5OCtr4KvctGufLbhxY7gc_W4TVlPqSrq8mWjo520z-Cqx431Zdz5pzluT9AHAy7p08M8Rqub5er6W33__fbu-uq-VryluaYCGqnUOGopMdUCWmZgYEqqUXGOh4FhbgSjMHDC1Dg0hWw63nRCiGFs2TH6so_dxPA865T7ySalnQOvw5x6QalshKD_BUlHWonlDiR7UMWQUtSm35QNIW57gvtd5f0_lRfP50P4PEx6_OM4FFyAswMASYEzEbyy6a9kSiTb5VzuMetNiBP8DNGNfYatC_G3h731jYtX9rUGl9cKou6fwhzLcdIbS_wCXE-tWQ</recordid><startdate>20000101</startdate><enddate>20000101</enddate><creator>Taskiran, Dilek</creator><creator>Tanyalcin, Then</creator><creator>Sozmen, Eser Y.</creator><creator>Peker, Gonul O.</creator><creator>Gulmen, Vehbi</creator><creator>Cagli, Sedat</creator><creator>Kanit, Luttfiye</creator><creator>Tekeli, Gurkan</creator><creator>Barcin, Erol</creator><creator>Zileli, Mehmet</creator><creator>Kutay, Fatma Z.</creator><general>Informa UK Ltd</general><general>Taylor & Francis</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>20000101</creationdate><title>The Effects of Melatonin on the Antioxidant Systems in Experimental Spinal Injury</title><author>Taskiran, Dilek ; Tanyalcin, Then ; Sozmen, Eser Y. ; Peker, Gonul O. ; Gulmen, Vehbi ; Cagli, Sedat ; Kanit, Luttfiye ; Tekeli, Gurkan ; Barcin, Erol ; Zileli, Mehmet ; Kutay, Fatma Z.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c462t-27a59ccdde9902e7a63fab3c9cdc440bb304f732ab413cdb5dde58458777bd63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>catalase (CAT)</topic><topic>Catalase - metabolism</topic><topic>Central nervous system</topic><topic>Central neurotransmission. Neuromudulation. Pathways and receptors</topic><topic>Clip compression</topic><topic>Disease Models, Animal</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glutathione - metabolism</topic><topic>Humans</topic><topic>Male</topic><topic>melatonin</topic><topic>Melatonin - administration & dosage</topic><topic>Melatonin - pharmacology</topic><topic>Melatonin - therapeutic use</topic><topic>Neurons - drug effects</topic><topic>oxidized glutathione (GSSG)</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>reduced glutathione (GSH)</topic><topic>Spinal Cord Injuries - drug therapy</topic><topic>Spinal Cord Injuries - enzymology</topic><topic>superoxide dismutase (SOD)</topic><topic>Superoxide Dismutase - metabolism</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Taskiran, Dilek</creatorcontrib><creatorcontrib>Tanyalcin, Then</creatorcontrib><creatorcontrib>Sozmen, Eser Y.</creatorcontrib><creatorcontrib>Peker, Gonul O.</creatorcontrib><creatorcontrib>Gulmen, Vehbi</creatorcontrib><creatorcontrib>Cagli, Sedat</creatorcontrib><creatorcontrib>Kanit, Luttfiye</creatorcontrib><creatorcontrib>Tekeli, Gurkan</creatorcontrib><creatorcontrib>Barcin, Erol</creatorcontrib><creatorcontrib>Zileli, Mehmet</creatorcontrib><creatorcontrib>Kutay, Fatma Z.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of neuroscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Taskiran, Dilek</au><au>Tanyalcin, Then</au><au>Sozmen, Eser Y.</au><au>Peker, Gonul O.</au><au>Gulmen, Vehbi</au><au>Cagli, Sedat</au><au>Kanit, Luttfiye</au><au>Tekeli, Gurkan</au><au>Barcin, Erol</au><au>Zileli, Mehmet</au><au>Kutay, Fatma Z.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Effects of Melatonin on the Antioxidant Systems in Experimental Spinal Injury</atitle><jtitle>International journal of neuroscience</jtitle><addtitle>Int J Neurosci</addtitle><date>2000-01-01</date><risdate>2000</risdate><volume>104</volume><issue>1</issue><spage>63</spage><epage>73</epage><pages>63-73</pages><issn>0020-7454</issn><eissn>1563-5279</eissn><eissn>1543-5245</eissn><coden>IJNUB7</coden><abstract>Melatonin has been recently shown by various in-vivo and in-vitro studies to exert potent neutralising effects on hydroxyl radicals, stimulate glutathione peroxidase (GSH-Px) activity, and protect catalase (CAT) from the destructive activity of hydroxyl radicals in neural tissue. We aimed to investigate the possible effects of pharmacological dose of melatonin on some of the antioxidant defence systems in an in-vivo study of experimental spinal injury. Seven groups of adult male Sprague Dawley rats were used in the following scheme: Group I: Naïve (n = 6), Group II: Lesion (n = 8), Group III: Melatonin (n = 5), Group IV: Melatonin + Lesion (n = 8), Group V: Placebo + Lesion (n = 5), Group VI: Sham operation (n = 5), and Group VII: Placebo (n = 5). Experimental spinal injury was induced at level T7-T8 by 5 sec compression of the total cord with an aneurism clip on anaesthetised and laminectomized animals. The total 10mg/kg dose of melatonin (Sigma) dissolved in alcohol-water was administered i.p. four times in 2.5 mg/kg doses, at 20min pre-, at the time of and at 1h and 2h post-compression. At 24±2h post-injury, the rats were euthanized and the lesioned segments of cord were dissected and homogenised with special care taken to distribute equal amount of injured tissue in each sample for analysis of reduced glutathione (GSH), oxidised glutathione (GSSG), superoxide dismutase (SOD), and CAT activity. Compression injury decreased GSH/GSSG ratio significantly (p <. 0001). Melatonin, by itself, significantly decreased GSSG content (p <. 05) and increased CAT activity (p <. 05) in the naive rats. Melatonin treatment decreased GSSG activity, thus elevating GSH/GSSG ratio, and also increased SOD and CAT activity without reaching statistical significance in the lesioned animals. In conclusion, pharmacological dose of systemically applied melatonin seemed to support some features of the antioxidant defence systems in our hands.</abstract><cop>London</cop><pub>Informa UK Ltd</pub><pmid>11011974</pmid><doi>10.3109/00207450009035009</doi><tpages>11</tpages></addata></record> |
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| subjects | Animals Biological and medical sciences catalase (CAT) Catalase - metabolism Central nervous system Central neurotransmission. Neuromudulation. Pathways and receptors Clip compression Disease Models, Animal Fundamental and applied biological sciences. Psychology Glutathione - metabolism Humans Male melatonin Melatonin - administration & dosage Melatonin - pharmacology Melatonin - therapeutic use Neurons - drug effects oxidized glutathione (GSSG) Rats Rats, Sprague-Dawley reduced glutathione (GSH) Spinal Cord Injuries - drug therapy Spinal Cord Injuries - enzymology superoxide dismutase (SOD) Superoxide Dismutase - metabolism Vertebrates: nervous system and sense organs |
| title | The Effects of Melatonin on the Antioxidant Systems in Experimental Spinal Injury |
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