Antioxidant Effect of Melatonin in Human Retinal Neuron Cultures
This study investigates whether the neurohormone melatonin can prevent the retinal neuronal injury caused by reactive oxygen species (ROS) in cultured human retinal neuronal cells. Cultures of human retinal neuronal cells established from a variety of donors were grown to 14 days and then subjected...
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| Veröffentlicht in: | Experimental neurology 2001-12, Vol.172 (2), p.407-415 |
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| creator | Lee, Min-Cheol Chung, Young-Taek Lee, Jae-Hyuk Jung, Jong-Jae Kim, Hyung-Seok Kim, Seung U. |
| description | This study investigates whether the neurohormone melatonin can prevent the retinal neuronal injury caused by reactive oxygen species (ROS) in cultured human retinal neuronal cells. Cultures of human retinal neuronal cells established from a variety of donors were grown to 14 days and then subjected to experimental hypoxanthine/xanthine oxidase (HX/XO)-induced injury. Intracellular production of ROS by administration of HX/XO was confirmed by flow cytometry; the ROS resulted in both apoptotic and necrotic pattern of cell death in the retinal neuron cultures. The efficacy of melatonin against ROS injury was quantitated by MTT assay, enzyme immunoassay, and immunocytochemistry for neurofilament protein. The antioxidative effect of melatonin was compared with that of α-tocopherol. Retinal neuronal injury significantly reduced in a dose–response manner by a treatment of 1.0–8.0 mM α-tocopherol. Melatonin, in concentrations of more than 2.0 mM, also significantly reduced the injury. About 70% of cells are rescued by pretreatment with 1.0 mM α-tocopherol and 8.0 mM melatonin in the MTT assay. Our observations suggest that melatonin can rescue retinal neurons from ROS injury in human retinal cell cultures. |
| doi_str_mv | 10.1006/exnr.2001.7793 |
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Cultures of human retinal neuronal cells established from a variety of donors were grown to 14 days and then subjected to experimental hypoxanthine/xanthine oxidase (HX/XO)-induced injury. Intracellular production of ROS by administration of HX/XO was confirmed by flow cytometry; the ROS resulted in both apoptotic and necrotic pattern of cell death in the retinal neuron cultures. The efficacy of melatonin against ROS injury was quantitated by MTT assay, enzyme immunoassay, and immunocytochemistry for neurofilament protein. The antioxidative effect of melatonin was compared with that of α-tocopherol. Retinal neuronal injury significantly reduced in a dose–response manner by a treatment of 1.0–8.0 mM α-tocopherol. Melatonin, in concentrations of more than 2.0 mM, also significantly reduced the injury. About 70% of cells are rescued by pretreatment with 1.0 mM α-tocopherol and 8.0 mM melatonin in the MTT assay. Our observations suggest that melatonin can rescue retinal neurons from ROS injury in human retinal cell cultures.</description><identifier>ISSN: 0014-4886</identifier><identifier>EISSN: 1090-2430</identifier><identifier>DOI: 10.1006/exnr.2001.7793</identifier><identifier>PMID: 11716564</identifier><identifier>CODEN: EXNEAC</identifier><language>eng</language><publisher>Amsterdam: Elsevier Inc</publisher><subject>Adult ; alpha-Tocopherol - pharmacology ; antioxidant ; Antioxidants - pharmacology ; Biological and medical sciences ; Cell Survival - drug effects ; Cells, Cultured ; DNA - metabolism ; Dose-Response Relationship, Drug ; Electrophoresis, Agar Gel ; Female ; Humans ; Hypoxanthine - pharmacology ; Male ; Medical sciences ; melatonin ; Melatonin - pharmacology ; MTT assay ; neurofilament protein ; Neurons - cytology ; Neurons - drug effects ; Neuropharmacology ; Neuroprotective agent ; Neuroprotective Agents - pharmacology ; Pharmacology. Drug treatments ; Reactive Oxygen Species - metabolism ; Reactive Oxygen Species - pharmacology ; Retina - cytology ; Retina - drug effects ; retinal neurons ; ROS (reactive oxygen species) ; Xanthine Oxidase - pharmacology</subject><ispartof>Experimental neurology, 2001-12, Vol.172 (2), p.407-415</ispartof><rights>2001 Elsevier Science (USA)</rights><rights>2002 INIST-CNRS</rights><rights>(c)2001 Elsevier Science.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c505t-36295f1ce5315ee6d78a9c6b8a21db66ca1717432324afc6fad81d0327cb3b793</citedby><cites>FETCH-LOGICAL-c505t-36295f1ce5315ee6d78a9c6b8a21db66ca1717432324afc6fad81d0327cb3b793</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0014488601977933$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13450085$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11716564$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lee, Min-Cheol</creatorcontrib><creatorcontrib>Chung, Young-Taek</creatorcontrib><creatorcontrib>Lee, Jae-Hyuk</creatorcontrib><creatorcontrib>Jung, Jong-Jae</creatorcontrib><creatorcontrib>Kim, Hyung-Seok</creatorcontrib><creatorcontrib>Kim, Seung U.</creatorcontrib><title>Antioxidant Effect of Melatonin in Human Retinal Neuron Cultures</title><title>Experimental neurology</title><addtitle>Exp Neurol</addtitle><description>This study investigates whether the neurohormone melatonin can prevent the retinal neuronal injury caused by reactive oxygen species (ROS) in cultured human retinal neuronal cells. Cultures of human retinal neuronal cells established from a variety of donors were grown to 14 days and then subjected to experimental hypoxanthine/xanthine oxidase (HX/XO)-induced injury. Intracellular production of ROS by administration of HX/XO was confirmed by flow cytometry; the ROS resulted in both apoptotic and necrotic pattern of cell death in the retinal neuron cultures. The efficacy of melatonin against ROS injury was quantitated by MTT assay, enzyme immunoassay, and immunocytochemistry for neurofilament protein. The antioxidative effect of melatonin was compared with that of α-tocopherol. Retinal neuronal injury significantly reduced in a dose–response manner by a treatment of 1.0–8.0 mM α-tocopherol. Melatonin, in concentrations of more than 2.0 mM, also significantly reduced the injury. About 70% of cells are rescued by pretreatment with 1.0 mM α-tocopherol and 8.0 mM melatonin in the MTT assay. Our observations suggest that melatonin can rescue retinal neurons from ROS injury in human retinal cell cultures.</description><subject>Adult</subject><subject>alpha-Tocopherol - pharmacology</subject><subject>antioxidant</subject><subject>Antioxidants - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Cell Survival - drug effects</subject><subject>Cells, Cultured</subject><subject>DNA - metabolism</subject><subject>Dose-Response Relationship, Drug</subject><subject>Electrophoresis, Agar Gel</subject><subject>Female</subject><subject>Humans</subject><subject>Hypoxanthine - pharmacology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>melatonin</subject><subject>Melatonin - pharmacology</subject><subject>MTT assay</subject><subject>neurofilament protein</subject><subject>Neurons - cytology</subject><subject>Neurons - drug effects</subject><subject>Neuropharmacology</subject><subject>Neuroprotective agent</subject><subject>Neuroprotective Agents - pharmacology</subject><subject>Pharmacology. Drug treatments</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Reactive Oxygen Species - pharmacology</subject><subject>Retina - cytology</subject><subject>Retina - drug effects</subject><subject>retinal neurons</subject><subject>ROS (reactive oxygen species)</subject><subject>Xanthine Oxidase - pharmacology</subject><issn>0014-4886</issn><issn>1090-2430</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kM1LwzAUwIMobk6vHqUXvXXmo0nbm2NMJ0wF0XNI0xeItOlMWpn_vRkr7CQ8ePD4va8fQtcEzwnG4h52zs8pxmSe5yU7QVOCS5zSjOFTNI3lLM2KQkzQRQhfGOMyo_k5mhCSE8FFNkUPC9fbbmdr5fpkZQzoPulM8gKN6jtnXRJjPbTKJe_QW6ea5BUG37lkOTT94CFcojOjmgBXY56hz8fVx3Kdbt6enpeLTao55n3KBC25IRo4IxxA1HmhSi2qQlFSV0JoFU_KM0YZzZTRwqi6IDVmNNcVq-JrM3R3mLv13fcAoZetDRqaRjnohiBzGhcwTiI4P4DadyF4MHLrbav8ryRY7p3JvTO5dyb3zmLDzTh5qFqoj_goKQK3I6CCVo3xymkbjhzLOMYFj1xx4CB6-LHgZdAWnIba-uhV1p3974Y_QeyHLA</recordid><startdate>20011201</startdate><enddate>20011201</enddate><creator>Lee, Min-Cheol</creator><creator>Chung, Young-Taek</creator><creator>Lee, Jae-Hyuk</creator><creator>Jung, Jong-Jae</creator><creator>Kim, Hyung-Seok</creator><creator>Kim, Seung U.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20011201</creationdate><title>Antioxidant Effect of Melatonin in Human Retinal Neuron Cultures</title><author>Lee, Min-Cheol ; Chung, Young-Taek ; Lee, Jae-Hyuk ; Jung, Jong-Jae ; Kim, Hyung-Seok ; Kim, Seung U.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c505t-36295f1ce5315ee6d78a9c6b8a21db66ca1717432324afc6fad81d0327cb3b793</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Adult</topic><topic>alpha-Tocopherol - pharmacology</topic><topic>antioxidant</topic><topic>Antioxidants - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Cell Survival - drug effects</topic><topic>Cells, Cultured</topic><topic>DNA - metabolism</topic><topic>Dose-Response Relationship, Drug</topic><topic>Electrophoresis, Agar Gel</topic><topic>Female</topic><topic>Humans</topic><topic>Hypoxanthine - pharmacology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>melatonin</topic><topic>Melatonin - pharmacology</topic><topic>MTT assay</topic><topic>neurofilament protein</topic><topic>Neurons - cytology</topic><topic>Neurons - drug effects</topic><topic>Neuropharmacology</topic><topic>Neuroprotective agent</topic><topic>Neuroprotective Agents - pharmacology</topic><topic>Pharmacology. Drug treatments</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>Reactive Oxygen Species - pharmacology</topic><topic>Retina - cytology</topic><topic>Retina - drug effects</topic><topic>retinal neurons</topic><topic>ROS (reactive oxygen species)</topic><topic>Xanthine Oxidase - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lee, Min-Cheol</creatorcontrib><creatorcontrib>Chung, Young-Taek</creatorcontrib><creatorcontrib>Lee, Jae-Hyuk</creatorcontrib><creatorcontrib>Jung, Jong-Jae</creatorcontrib><creatorcontrib>Kim, Hyung-Seok</creatorcontrib><creatorcontrib>Kim, Seung U.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental neurology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lee, Min-Cheol</au><au>Chung, Young-Taek</au><au>Lee, Jae-Hyuk</au><au>Jung, Jong-Jae</au><au>Kim, Hyung-Seok</au><au>Kim, Seung U.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antioxidant Effect of Melatonin in Human Retinal Neuron Cultures</atitle><jtitle>Experimental neurology</jtitle><addtitle>Exp Neurol</addtitle><date>2001-12-01</date><risdate>2001</risdate><volume>172</volume><issue>2</issue><spage>407</spage><epage>415</epage><pages>407-415</pages><issn>0014-4886</issn><eissn>1090-2430</eissn><coden>EXNEAC</coden><abstract>This study investigates whether the neurohormone melatonin can prevent the retinal neuronal injury caused by reactive oxygen species (ROS) in cultured human retinal neuronal cells. Cultures of human retinal neuronal cells established from a variety of donors were grown to 14 days and then subjected to experimental hypoxanthine/xanthine oxidase (HX/XO)-induced injury. Intracellular production of ROS by administration of HX/XO was confirmed by flow cytometry; the ROS resulted in both apoptotic and necrotic pattern of cell death in the retinal neuron cultures. The efficacy of melatonin against ROS injury was quantitated by MTT assay, enzyme immunoassay, and immunocytochemistry for neurofilament protein. The antioxidative effect of melatonin was compared with that of α-tocopherol. Retinal neuronal injury significantly reduced in a dose–response manner by a treatment of 1.0–8.0 mM α-tocopherol. Melatonin, in concentrations of more than 2.0 mM, also significantly reduced the injury. About 70% of cells are rescued by pretreatment with 1.0 mM α-tocopherol and 8.0 mM melatonin in the MTT assay. Our observations suggest that melatonin can rescue retinal neurons from ROS injury in human retinal cell cultures.</abstract><cop>Amsterdam</cop><pub>Elsevier Inc</pub><pmid>11716564</pmid><doi>10.1006/exnr.2001.7793</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adult alpha-Tocopherol - pharmacology antioxidant Antioxidants - pharmacology Biological and medical sciences Cell Survival - drug effects Cells, Cultured DNA - metabolism Dose-Response Relationship, Drug Electrophoresis, Agar Gel Female Humans Hypoxanthine - pharmacology Male Medical sciences melatonin Melatonin - pharmacology MTT assay neurofilament protein Neurons - cytology Neurons - drug effects Neuropharmacology Neuroprotective agent Neuroprotective Agents - pharmacology Pharmacology. Drug treatments Reactive Oxygen Species - metabolism Reactive Oxygen Species - pharmacology Retina - cytology Retina - drug effects retinal neurons ROS (reactive oxygen species) Xanthine Oxidase - pharmacology |
| title | Antioxidant Effect of Melatonin in Human Retinal Neuron Cultures |
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