Potential use of Amplicor PCR kit in diagnosing pulmonary tuberculosis from gastric aspirate
Polymerase chain reaction (PCR) detection of mycobacteria from gastric aspirate for the diagnosis of tuberculosis is not fully evaluated up to now. A total of 116 gastric aspirate specimens were collected from patients with suspected pulmonary tuberculosis. The breakdown of diagnosis was 67 pulmonar...
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| Veröffentlicht in: | Journal of microbiological methods 2001-12, Vol.47 (3), p.339-344 |
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| container_title | Journal of microbiological methods |
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| creator | Mitarai, S Tanoue, S Sugita, C Sugihara, E Tamura, A Nagono, Y Tsuboi, M Nagayama, N Kurashima, A Nagai, H Shishido, H |
| description | Polymerase chain reaction (PCR) detection of mycobacteria from gastric aspirate for the diagnosis of tuberculosis is not fully evaluated up to now. A total of 116 gastric aspirate specimens were collected from patients with suspected pulmonary tuberculosis. The breakdown of diagnosis was 67 pulmonary tuberculosis, 16 nontuberculous mycobacterial infection, 5 extra pulmonary tuberculosis, and 28 other lung diseases. The conventional methods were shown to have a sensitivity of 47.8% and a specificity of 79.6%; on the other hand, Amplicor had 34.9% and 97.0%, respectively. The Amplicor provided a more rapid and specific method for diagnosing tuberculosis and was more useful than the conventional. |
| doi_str_mv | 10.1016/S0167-7012(01)00341-4 |
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A total of 116 gastric aspirate specimens were collected from patients with suspected pulmonary tuberculosis. The breakdown of diagnosis was 67 pulmonary tuberculosis, 16 nontuberculous mycobacterial infection, 5 extra pulmonary tuberculosis, and 28 other lung diseases. The conventional methods were shown to have a sensitivity of 47.8% and a specificity of 79.6%; on the other hand, Amplicor had 34.9% and 97.0%, respectively. 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Psychology ; Gastric aspirate ; Gastric Juice - microbiology ; Gene Amplification ; Human bacterial diseases ; Humans ; Infectious diseases ; Male ; Medical sciences ; Microbiology ; Middle Aged ; Mycobacterium tuberculosis ; Mycobacterium tuberculosis - genetics ; Mycobacterium tuberculosis - isolation & purification ; Polymerase Chain Reaction - methods ; Reagent Kits, Diagnostic ; Sensitivity and Specificity ; Tuberculosis ; Tuberculosis and atypical mycobacterial infections ; Tuberculosis, Pulmonary - diagnosis ; Tuberculosis, Pulmonary - microbiology</subject><ispartof>Journal of microbiological methods, 2001-12, Vol.47 (3), p.339-344</ispartof><rights>2001 Elsevier Science B.V.</rights><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c422t-2b9906715b78cae2d07b279ae3737af9e81b3e79b4759fdc6411e66eef21da123</citedby><cites>FETCH-LOGICAL-c422t-2b9906715b78cae2d07b279ae3737af9e81b3e79b4759fdc6411e66eef21da123</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0167701201003414$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14163452$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11714524$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mitarai, S</creatorcontrib><creatorcontrib>Tanoue, S</creatorcontrib><creatorcontrib>Sugita, C</creatorcontrib><creatorcontrib>Sugihara, E</creatorcontrib><creatorcontrib>Tamura, A</creatorcontrib><creatorcontrib>Nagono, Y</creatorcontrib><creatorcontrib>Tsuboi, M</creatorcontrib><creatorcontrib>Nagayama, N</creatorcontrib><creatorcontrib>Kurashima, A</creatorcontrib><creatorcontrib>Nagai, H</creatorcontrib><creatorcontrib>Shishido, H</creatorcontrib><title>Potential use of Amplicor PCR kit in diagnosing pulmonary tuberculosis from gastric aspirate</title><title>Journal of microbiological methods</title><addtitle>J Microbiol Methods</addtitle><description>Polymerase chain reaction (PCR) detection of mycobacteria from gastric aspirate for the diagnosis of tuberculosis is not fully evaluated up to now. A total of 116 gastric aspirate specimens were collected from patients with suspected pulmonary tuberculosis. The breakdown of diagnosis was 67 pulmonary tuberculosis, 16 nontuberculous mycobacterial infection, 5 extra pulmonary tuberculosis, and 28 other lung diseases. The conventional methods were shown to have a sensitivity of 47.8% and a specificity of 79.6%; on the other hand, Amplicor had 34.9% and 97.0%, respectively. The Amplicor provided a more rapid and specific method for diagnosing tuberculosis and was more useful than the conventional.</description><subject>Adult</subject><subject>Aged</subject><subject>Amplicor</subject><subject>Bacterial diseases</subject><subject>Bacteriological methods and techniques used in bacteriology</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Diagnosis</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gastric aspirate</subject><subject>Gastric Juice - microbiology</subject><subject>Gene Amplification</subject><subject>Human bacterial diseases</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Microbiology</subject><subject>Middle Aged</subject><subject>Mycobacterium tuberculosis</subject><subject>Mycobacterium tuberculosis - genetics</subject><subject>Mycobacterium tuberculosis - isolation & purification</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Reagent Kits, Diagnostic</subject><subject>Sensitivity and Specificity</subject><subject>Tuberculosis</subject><subject>Tuberculosis and atypical mycobacterial infections</subject><subject>Tuberculosis, Pulmonary - diagnosis</subject><subject>Tuberculosis, Pulmonary - microbiology</subject><issn>0167-7012</issn><issn>1872-8359</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0F1L3TAYwPEgDj1z-wgbuVG2i2qeJG3aq3E4bHMgKG67G4Q0fXrI1jZdkgp-e6PnoJfeJBB-efsT8gHYOTCoLn7mQRWKAf_E4DNjQkIhD8gKasWLWpTNIVk9k2PyNsa_jEEpZH1EjgEUyJLLFflz4xNOyZmBLhGp7-l6nAdnfaA3m1v6zyXqJto5s518dNOWzssw-smEe5qWFoNdhrweaR_8SLcmpuAsNXF2wSR8R970Zoj4fj-fkN_fvv7aXBZX199_bNZXhZWcp4K3TcMqBWWramuQd0y1XDUGhRLK9A3W0ApUTStV2fSdrSQAVhViz6EzwMUJOdudOwf_f8GY9OiixWEwE_olasV5I2olXoVQg8gpWYblDtrgYwzY6zm4Mf9aA9OP_fVTf_0YVzPQT_21zPs-7i9Y2hG7l1374Bmc7oGJ1gx9MJN18cVJqESG2X3ZOczd7hwGHa3DyWLnAtqkO-9eecoDIh2h4A</recordid><startdate>20011201</startdate><enddate>20011201</enddate><creator>Mitarai, S</creator><creator>Tanoue, S</creator><creator>Sugita, C</creator><creator>Sugihara, E</creator><creator>Tamura, A</creator><creator>Nagono, Y</creator><creator>Tsuboi, M</creator><creator>Nagayama, N</creator><creator>Kurashima, A</creator><creator>Nagai, H</creator><creator>Shishido, H</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20011201</creationdate><title>Potential use of Amplicor PCR kit in diagnosing pulmonary tuberculosis from gastric aspirate</title><author>Mitarai, S ; Tanoue, S ; Sugita, C ; Sugihara, E ; Tamura, A ; Nagono, Y ; Tsuboi, M ; Nagayama, N ; Kurashima, A ; Nagai, H ; Shishido, H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-2b9906715b78cae2d07b279ae3737af9e81b3e79b4759fdc6411e66eef21da123</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Amplicor</topic><topic>Bacterial diseases</topic><topic>Bacteriological methods and techniques used in bacteriology</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Diagnosis</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gastric aspirate</topic><topic>Gastric Juice - microbiology</topic><topic>Gene Amplification</topic><topic>Human bacterial diseases</topic><topic>Humans</topic><topic>Infectious diseases</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Microbiology</topic><topic>Middle Aged</topic><topic>Mycobacterium tuberculosis</topic><topic>Mycobacterium tuberculosis - genetics</topic><topic>Mycobacterium tuberculosis - isolation & purification</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Reagent Kits, Diagnostic</topic><topic>Sensitivity and Specificity</topic><topic>Tuberculosis</topic><topic>Tuberculosis and atypical mycobacterial infections</topic><topic>Tuberculosis, Pulmonary - diagnosis</topic><topic>Tuberculosis, Pulmonary - microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mitarai, S</creatorcontrib><creatorcontrib>Tanoue, S</creatorcontrib><creatorcontrib>Sugita, C</creatorcontrib><creatorcontrib>Sugihara, E</creatorcontrib><creatorcontrib>Tamura, A</creatorcontrib><creatorcontrib>Nagono, Y</creatorcontrib><creatorcontrib>Tsuboi, M</creatorcontrib><creatorcontrib>Nagayama, N</creatorcontrib><creatorcontrib>Kurashima, A</creatorcontrib><creatorcontrib>Nagai, H</creatorcontrib><creatorcontrib>Shishido, H</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of microbiological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mitarai, S</au><au>Tanoue, S</au><au>Sugita, C</au><au>Sugihara, E</au><au>Tamura, A</au><au>Nagono, Y</au><au>Tsuboi, M</au><au>Nagayama, N</au><au>Kurashima, A</au><au>Nagai, H</au><au>Shishido, H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Potential use of Amplicor PCR kit in diagnosing pulmonary tuberculosis from gastric aspirate</atitle><jtitle>Journal of microbiological methods</jtitle><addtitle>J Microbiol Methods</addtitle><date>2001-12-01</date><risdate>2001</risdate><volume>47</volume><issue>3</issue><spage>339</spage><epage>344</epage><pages>339-344</pages><issn>0167-7012</issn><eissn>1872-8359</eissn><coden>JMIMDQ</coden><abstract>Polymerase chain reaction (PCR) detection of mycobacteria from gastric aspirate for the diagnosis of tuberculosis is not fully evaluated up to now. A total of 116 gastric aspirate specimens were collected from patients with suspected pulmonary tuberculosis. The breakdown of diagnosis was 67 pulmonary tuberculosis, 16 nontuberculous mycobacterial infection, 5 extra pulmonary tuberculosis, and 28 other lung diseases. The conventional methods were shown to have a sensitivity of 47.8% and a specificity of 79.6%; on the other hand, Amplicor had 34.9% and 97.0%, respectively. The Amplicor provided a more rapid and specific method for diagnosing tuberculosis and was more useful than the conventional.</abstract><cop>Shannon</cop><pub>Elsevier B.V</pub><pmid>11714524</pmid><doi>10.1016/S0167-7012(01)00341-4</doi><tpages>6</tpages></addata></record> |
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| subjects | Adult Aged Amplicor Bacterial diseases Bacteriological methods and techniques used in bacteriology Bacteriology Biological and medical sciences Diagnosis Female Fundamental and applied biological sciences. Psychology Gastric aspirate Gastric Juice - microbiology Gene Amplification Human bacterial diseases Humans Infectious diseases Male Medical sciences Microbiology Middle Aged Mycobacterium tuberculosis Mycobacterium tuberculosis - genetics Mycobacterium tuberculosis - isolation & purification Polymerase Chain Reaction - methods Reagent Kits, Diagnostic Sensitivity and Specificity Tuberculosis Tuberculosis and atypical mycobacterial infections Tuberculosis, Pulmonary - diagnosis Tuberculosis, Pulmonary - microbiology |
| title | Potential use of Amplicor PCR kit in diagnosing pulmonary tuberculosis from gastric aspirate |
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