PACAP has a neurotrophic effect on cultured basal forebrain cholinergic neurons from adult rats
Pituitary adenylate cyclase-activating polypeptide (PACAP) is a member of the vasoactive intestinal polypeptide gene family (VIP) that was originally isolated from rat hypothalamus. The high affinity PACAP receptor, PAC1, is expressed in the basal forebrain area of adult, as well as developing rat b...
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| Veröffentlicht in: | Brain research. Developmental brain research 2001-11, Vol.131 (1), p.41-45 |
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| container_title | Brain research. Developmental brain research |
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| creator | Yuhara, Aki Nishio, Chika Abiru, Yasuhiro Hatanaka, Hiroshi Takei, Nobuyuki |
| description | Pituitary adenylate cyclase-activating polypeptide (PACAP) is a member of the vasoactive intestinal polypeptide gene family (VIP) that was originally isolated from rat hypothalamus. The high affinity PACAP receptor, PAC1, is expressed in the basal forebrain area of adult, as well as developing rat brain. Hippocampus, a targeting area of basal forebrain cholinergic neurons, contains PACAP. Thus, hippocampal-derived PACAP may have an effect on basal forebrain cholinergic neurons. Indeed, we have reported that PACAP showed neurotrophic effects on these neurons in embryonic and early postnatal culture. Here we report that PACAP has a neurotrophic effect on adult cholinergic neurons in culture. PACAP increases the number of choline acetyltransferase immunoreactive neurons about 2-fold. A similar effect was observed on treatment with cAMP analogue but not nerve growth factor. PACAP also improved the survival and neurite outgrowth of total neurons. These results indicate that PACAP acts as a neurotophic factor even on adult neurons in vitro. |
| doi_str_mv | 10.1016/S0165-3806(01)00249-8 |
| format | Article |
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The high affinity PACAP receptor, PAC1, is expressed in the basal forebrain area of adult, as well as developing rat brain. Hippocampus, a targeting area of basal forebrain cholinergic neurons, contains PACAP. Thus, hippocampal-derived PACAP may have an effect on basal forebrain cholinergic neurons. Indeed, we have reported that PACAP showed neurotrophic effects on these neurons in embryonic and early postnatal culture. Here we report that PACAP has a neurotrophic effect on adult cholinergic neurons in culture. PACAP increases the number of choline acetyltransferase immunoreactive neurons about 2-fold. A similar effect was observed on treatment with cAMP analogue but not nerve growth factor. PACAP also improved the survival and neurite outgrowth of total neurons. These results indicate that PACAP acts as a neurotophic factor even on adult neurons in vitro.</description><identifier>ISSN: 0165-3806</identifier><identifier>DOI: 10.1016/S0165-3806(01)00249-8</identifier><identifier>PMID: 11718834</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Adult neuron in culture ; Age Factors ; Alzheimer’s disease ; Animals ; Basal forebrain cholinergic neuron ; Cell Death - drug effects ; Cell Survival - drug effects ; Cells, Cultured ; Choline O-Acetyltransferase - analysis ; Cholinergic Fibers - drug effects ; Cholinergic Fibers - physiology ; Cyclic AMP - analogs & derivatives ; Cyclic AMP - pharmacology ; Male ; Mice ; Nerve Growth Factor - pharmacology ; Neuronal cell death ; Neurons - drug effects ; Neurons - enzymology ; Neurons - ultrastructure ; Neuropeptides - pharmacology ; Neuroprotective Agents - pharmacology ; Neurotrophin ; NGF ; PACAP ; Pituitary Adenylate Cyclase-Activating Polypeptide ; Prosencephalon - cytology ; Thionucleotides - pharmacology</subject><ispartof>Brain research. Developmental brain research, 2001-11, Vol.131 (1), p.41-45</ispartof><rights>2001 Elsevier Science B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c444t-2c4f95d3c7106c26d14e8da4ed4a0f36197c179c320adbba65209eae40dc385e3</citedby><cites>FETCH-LOGICAL-c444t-2c4f95d3c7106c26d14e8da4ed4a0f36197c179c320adbba65209eae40dc385e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11718834$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yuhara, Aki</creatorcontrib><creatorcontrib>Nishio, Chika</creatorcontrib><creatorcontrib>Abiru, Yasuhiro</creatorcontrib><creatorcontrib>Hatanaka, Hiroshi</creatorcontrib><creatorcontrib>Takei, Nobuyuki</creatorcontrib><title>PACAP has a neurotrophic effect on cultured basal forebrain cholinergic neurons from adult rats</title><title>Brain research. Developmental brain research</title><addtitle>Brain Res Dev Brain Res</addtitle><description>Pituitary adenylate cyclase-activating polypeptide (PACAP) is a member of the vasoactive intestinal polypeptide gene family (VIP) that was originally isolated from rat hypothalamus. The high affinity PACAP receptor, PAC1, is expressed in the basal forebrain area of adult, as well as developing rat brain. Hippocampus, a targeting area of basal forebrain cholinergic neurons, contains PACAP. Thus, hippocampal-derived PACAP may have an effect on basal forebrain cholinergic neurons. Indeed, we have reported that PACAP showed neurotrophic effects on these neurons in embryonic and early postnatal culture. Here we report that PACAP has a neurotrophic effect on adult cholinergic neurons in culture. PACAP increases the number of choline acetyltransferase immunoreactive neurons about 2-fold. A similar effect was observed on treatment with cAMP analogue but not nerve growth factor. PACAP also improved the survival and neurite outgrowth of total neurons. These results indicate that PACAP acts as a neurotophic factor even on adult neurons in vitro.</description><subject>Adult neuron in culture</subject><subject>Age Factors</subject><subject>Alzheimer’s disease</subject><subject>Animals</subject><subject>Basal forebrain cholinergic neuron</subject><subject>Cell Death - drug effects</subject><subject>Cell Survival - drug effects</subject><subject>Cells, Cultured</subject><subject>Choline O-Acetyltransferase - analysis</subject><subject>Cholinergic Fibers - drug effects</subject><subject>Cholinergic Fibers - physiology</subject><subject>Cyclic AMP - analogs & derivatives</subject><subject>Cyclic AMP - pharmacology</subject><subject>Male</subject><subject>Mice</subject><subject>Nerve Growth Factor - pharmacology</subject><subject>Neuronal cell death</subject><subject>Neurons - drug effects</subject><subject>Neurons - enzymology</subject><subject>Neurons - ultrastructure</subject><subject>Neuropeptides - pharmacology</subject><subject>Neuroprotective Agents - pharmacology</subject><subject>Neurotrophin</subject><subject>NGF</subject><subject>PACAP</subject><subject>Pituitary Adenylate Cyclase-Activating Polypeptide</subject><subject>Prosencephalon - cytology</subject><subject>Thionucleotides - pharmacology</subject><issn>0165-3806</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1r3DAQhnVoyFfzE1J0CunB7Ywk2_IpLEuTFgINNDkLWRp3VbzWRrID-ff1fpAcc9GA5nnnhYexS4RvCFh9_zM_ZSE1VNeAXwGEagr9iZ2-fZ-ws5z_AQBKjcfsBLFGraU6ZeZhsVw88JXN3PKBphTHFDer4Dh1HbmRx4G7qR-nRJ63NtuedzFRm2yYF6vYh4HS3xnfZYfMuxTX3Po5wpMd82d21Nk-08VhnrOn2x-Py5_F_e-7X8vFfeGUUmMhnOqa0ktXI1ROVB4VaW8VeWWhkxU2tcO6cVKA9W1rq1JAQ5YUeCd1SfKcXe3vblJ8niiPZh2yo763A8Upm1qIRlagPwRRC0QpxAyWe9ClmHOizmxSWNv0ahDMVrvZaTdbvwbQ7LSbbcGXQ8HUrsm_pw7OZ-BmD9Ds4yVQMtkFGhz5kGbjxsfwQcV_jv6UZA</recordid><startdate>20011126</startdate><enddate>20011126</enddate><creator>Yuhara, Aki</creator><creator>Nishio, Chika</creator><creator>Abiru, Yasuhiro</creator><creator>Hatanaka, Hiroshi</creator><creator>Takei, Nobuyuki</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>20011126</creationdate><title>PACAP has a neurotrophic effect on cultured basal forebrain cholinergic neurons from adult rats</title><author>Yuhara, Aki ; Nishio, Chika ; Abiru, Yasuhiro ; Hatanaka, Hiroshi ; Takei, Nobuyuki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c444t-2c4f95d3c7106c26d14e8da4ed4a0f36197c179c320adbba65209eae40dc385e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Adult neuron in culture</topic><topic>Age Factors</topic><topic>Alzheimer’s disease</topic><topic>Animals</topic><topic>Basal forebrain cholinergic neuron</topic><topic>Cell Death - drug effects</topic><topic>Cell Survival - drug effects</topic><topic>Cells, Cultured</topic><topic>Choline O-Acetyltransferase - analysis</topic><topic>Cholinergic Fibers - drug effects</topic><topic>Cholinergic Fibers - physiology</topic><topic>Cyclic AMP - analogs & derivatives</topic><topic>Cyclic AMP - pharmacology</topic><topic>Male</topic><topic>Mice</topic><topic>Nerve Growth Factor - pharmacology</topic><topic>Neuronal cell death</topic><topic>Neurons - drug effects</topic><topic>Neurons - enzymology</topic><topic>Neurons - ultrastructure</topic><topic>Neuropeptides - pharmacology</topic><topic>Neuroprotective Agents - pharmacology</topic><topic>Neurotrophin</topic><topic>NGF</topic><topic>PACAP</topic><topic>Pituitary Adenylate Cyclase-Activating Polypeptide</topic><topic>Prosencephalon - cytology</topic><topic>Thionucleotides - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yuhara, Aki</creatorcontrib><creatorcontrib>Nishio, Chika</creatorcontrib><creatorcontrib>Abiru, Yasuhiro</creatorcontrib><creatorcontrib>Hatanaka, Hiroshi</creatorcontrib><creatorcontrib>Takei, Nobuyuki</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Brain research. Developmental brain research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yuhara, Aki</au><au>Nishio, Chika</au><au>Abiru, Yasuhiro</au><au>Hatanaka, Hiroshi</au><au>Takei, Nobuyuki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>PACAP has a neurotrophic effect on cultured basal forebrain cholinergic neurons from adult rats</atitle><jtitle>Brain research. Developmental brain research</jtitle><addtitle>Brain Res Dev Brain Res</addtitle><date>2001-11-26</date><risdate>2001</risdate><volume>131</volume><issue>1</issue><spage>41</spage><epage>45</epage><pages>41-45</pages><issn>0165-3806</issn><abstract>Pituitary adenylate cyclase-activating polypeptide (PACAP) is a member of the vasoactive intestinal polypeptide gene family (VIP) that was originally isolated from rat hypothalamus. The high affinity PACAP receptor, PAC1, is expressed in the basal forebrain area of adult, as well as developing rat brain. Hippocampus, a targeting area of basal forebrain cholinergic neurons, contains PACAP. Thus, hippocampal-derived PACAP may have an effect on basal forebrain cholinergic neurons. Indeed, we have reported that PACAP showed neurotrophic effects on these neurons in embryonic and early postnatal culture. Here we report that PACAP has a neurotrophic effect on adult cholinergic neurons in culture. PACAP increases the number of choline acetyltransferase immunoreactive neurons about 2-fold. A similar effect was observed on treatment with cAMP analogue but not nerve growth factor. PACAP also improved the survival and neurite outgrowth of total neurons. 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| subjects | Adult neuron in culture Age Factors Alzheimer’s disease Animals Basal forebrain cholinergic neuron Cell Death - drug effects Cell Survival - drug effects Cells, Cultured Choline O-Acetyltransferase - analysis Cholinergic Fibers - drug effects Cholinergic Fibers - physiology Cyclic AMP - analogs & derivatives Cyclic AMP - pharmacology Male Mice Nerve Growth Factor - pharmacology Neuronal cell death Neurons - drug effects Neurons - enzymology Neurons - ultrastructure Neuropeptides - pharmacology Neuroprotective Agents - pharmacology Neurotrophin NGF PACAP Pituitary Adenylate Cyclase-Activating Polypeptide Prosencephalon - cytology Thionucleotides - pharmacology |
| title | PACAP has a neurotrophic effect on cultured basal forebrain cholinergic neurons from adult rats |
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