Packaging of Tobacco Mosaic Virus Subgenomic RNAs by Brome Mosaic Virus Coat Protein Exhibits RNA Controlled Polymorphism
The coat protein (CP) of icosahedral Brome mosaic virus (BMV) was expressed from a genetically engineered rod-shape Tobacco mosaic virus. Molecular characterization of the progeny recovered from symptomatic plants revealed that BMV CP selectively packaged the three subgenomic RNAs of the hybrid viru...
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| Veröffentlicht in: | Virology (New York, N.Y.) N.Y.), 2000-09, Vol.275 (2), p.249-257 |
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| container_title | Virology (New York, N.Y.) |
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| creator | Choi, Yoon Gi Rao, A.L.N. |
| description | The coat protein (CP) of icosahedral Brome mosaic virus (BMV) was expressed from a genetically engineered rod-shape Tobacco mosaic virus. Molecular characterization of the progeny recovered from symptomatic plants revealed that BMV CP selectively packaged the three subgenomic RNAs of the hybrid virus into two differently sized icosahedral virus-like particles (VLPs). The smaller VLPs packaged only the two smaller subgenomic RNAs. Additional in vitro reassembly assays with BMV CP subunits and transcripts of hybrid subgenomic RNAs further demonstrated that the ability of BMV capsids to display polymorphism is not dependent on the RNA size alone and appears to be controlled by some other feature(s) of the genetically engineered RNA. |
| doi_str_mv | 10.1006/viro.2000.0532 |
| format | Article |
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Molecular characterization of the progeny recovered from symptomatic plants revealed that BMV CP selectively packaged the three subgenomic RNAs of the hybrid virus into two differently sized icosahedral virus-like particles (VLPs). The smaller VLPs packaged only the two smaller subgenomic RNAs. Additional in vitro reassembly assays with BMV CP subunits and transcripts of hybrid subgenomic RNAs further demonstrated that the ability of BMV capsids to display polymorphism is not dependent on the RNA size alone and appears to be controlled by some other feature(s) of the genetically engineered RNA.</description><identifier>ISSN: 0042-6822</identifier><identifier>EISSN: 1096-0341</identifier><identifier>DOI: 10.1006/viro.2000.0532</identifier><identifier>PMID: 10998324</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Blotting, Northern ; Brome mosaic virus ; Bromovirus - metabolism ; Capsid - metabolism ; Chenopodiaceae - virology ; coat protein ; Polymorphism, Genetic ; Protein Binding ; RNA, Viral - metabolism ; Tobacco mosaic virus ; Tobacco Mosaic Virus - genetics ; Virion - metabolism ; Virus Assembly ; Xenopus</subject><ispartof>Virology (New York, N.Y.), 2000-09, Vol.275 (2), p.249-257</ispartof><rights>2000 Academic Press</rights><rights>Copyright 2000 Academic Press.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c411t-919065906d5d987246177cf225082b16f71a90f7f5c90a8cc6ba8b448a1c66e83</citedby><cites>FETCH-LOGICAL-c411t-919065906d5d987246177cf225082b16f71a90f7f5c90a8cc6ba8b448a1c66e83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/viro.2000.0532$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10998324$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Choi, Yoon Gi</creatorcontrib><creatorcontrib>Rao, A.L.N.</creatorcontrib><title>Packaging of Tobacco Mosaic Virus Subgenomic RNAs by Brome Mosaic Virus Coat Protein Exhibits RNA Controlled Polymorphism</title><title>Virology (New York, N.Y.)</title><addtitle>Virology</addtitle><description>The coat protein (CP) of icosahedral Brome mosaic virus (BMV) was expressed from a genetically engineered rod-shape Tobacco mosaic virus. Molecular characterization of the progeny recovered from symptomatic plants revealed that BMV CP selectively packaged the three subgenomic RNAs of the hybrid virus into two differently sized icosahedral virus-like particles (VLPs). The smaller VLPs packaged only the two smaller subgenomic RNAs. Additional in vitro reassembly assays with BMV CP subunits and transcripts of hybrid subgenomic RNAs further demonstrated that the ability of BMV capsids to display polymorphism is not dependent on the RNA size alone and appears to be controlled by some other feature(s) of the genetically engineered RNA.</description><subject>Animals</subject><subject>Blotting, Northern</subject><subject>Brome mosaic virus</subject><subject>Bromovirus - metabolism</subject><subject>Capsid - metabolism</subject><subject>Chenopodiaceae - virology</subject><subject>coat protein</subject><subject>Polymorphism, Genetic</subject><subject>Protein Binding</subject><subject>RNA, Viral - metabolism</subject><subject>Tobacco mosaic virus</subject><subject>Tobacco Mosaic Virus - genetics</subject><subject>Virion - metabolism</subject><subject>Virus Assembly</subject><subject>Xenopus</subject><issn>0042-6822</issn><issn>1096-0341</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkT1v2zAQhomgQex8rBkLTt3kHimKpMbESJMCbmLkayUoirLZSKJLSkb87yPBGdIhyHA44O65d7gHoXMCMwLAf25d8DMKADPIUnqApgRynkDKyDc0BWA04ZLSCTqO8e9AMSHgCE0GKJcpZVO0W2rzoleuXWFf4UdfaGM8_uOjdgY_u9BH_NAXK9v6Zhjc315EXOzwZfCN_Z-ae93hZfCddS2-el27wnVxPBg2bRd8XdsSL329a3zYrF1sTtFhpetoz977CXr6dfU4v0kWd9e_5xeLxDBCuiQnOfBsqDIrcyko40QIU1GagaQF4ZUgOodKVJnJQUtjeKFlwZjUxHBuZXqCfuxzN8H_623sVOOisXWtW-v7qASlkuci_RIkgnNgbARne9AEH2OwldoE1-iwUwTUaEWNVtRoRY1WhoPv78l90djyA77XMAByD9jhEVtng4rG2dbY0gVrOlV691n2G6E6mzo</recordid><startdate>20000930</startdate><enddate>20000930</enddate><creator>Choi, Yoon Gi</creator><creator>Rao, A.L.N.</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20000930</creationdate><title>Packaging of Tobacco Mosaic Virus Subgenomic RNAs by Brome Mosaic Virus Coat Protein Exhibits RNA Controlled Polymorphism</title><author>Choi, Yoon Gi ; Rao, A.L.N.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c411t-919065906d5d987246177cf225082b16f71a90f7f5c90a8cc6ba8b448a1c66e83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>Blotting, Northern</topic><topic>Brome mosaic virus</topic><topic>Bromovirus - metabolism</topic><topic>Capsid - metabolism</topic><topic>Chenopodiaceae - virology</topic><topic>coat protein</topic><topic>Polymorphism, Genetic</topic><topic>Protein Binding</topic><topic>RNA, Viral - metabolism</topic><topic>Tobacco mosaic virus</topic><topic>Tobacco Mosaic Virus - genetics</topic><topic>Virion - metabolism</topic><topic>Virus Assembly</topic><topic>Xenopus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Choi, Yoon Gi</creatorcontrib><creatorcontrib>Rao, A.L.N.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Virology (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Choi, Yoon Gi</au><au>Rao, A.L.N.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Packaging of Tobacco Mosaic Virus Subgenomic RNAs by Brome Mosaic Virus Coat Protein Exhibits RNA Controlled Polymorphism</atitle><jtitle>Virology (New York, N.Y.)</jtitle><addtitle>Virology</addtitle><date>2000-09-30</date><risdate>2000</risdate><volume>275</volume><issue>2</issue><spage>249</spage><epage>257</epage><pages>249-257</pages><issn>0042-6822</issn><eissn>1096-0341</eissn><abstract>The coat protein (CP) of icosahedral Brome mosaic virus (BMV) was expressed from a genetically engineered rod-shape Tobacco mosaic virus. 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| identifier | ISSN: 0042-6822 |
| ispartof | Virology (New York, N.Y.), 2000-09, Vol.275 (2), p.249-257 |
| issn | 0042-6822 1096-0341 |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Blotting, Northern Brome mosaic virus Bromovirus - metabolism Capsid - metabolism Chenopodiaceae - virology coat protein Polymorphism, Genetic Protein Binding RNA, Viral - metabolism Tobacco mosaic virus Tobacco Mosaic Virus - genetics Virion - metabolism Virus Assembly Xenopus |
| title | Packaging of Tobacco Mosaic Virus Subgenomic RNAs by Brome Mosaic Virus Coat Protein Exhibits RNA Controlled Polymorphism |
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