Performance of WSSV-infected and WSSV-negative Penaeus monodon postlarvae in culture ponds

In a survey of 27 Penaeus monodon culture ponds stocked with postlarvae (approximately PL10) at medium density (approximately 40 shrimp m(-2)), single-step nested white spot syndrome virus (WSSV) PCR was used to measure the WSSV infection rates in the shrimp populations within 1 mo after stocking. S...

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Veröffentlicht in:Diseases of aquatic organisms 2001-10, Vol.46 (3), p.165-172
Hauptverfasser: PENG, Shao-En, LO, Chu-Fang, LIN, San-Ching, CHEN, Li-Li, CHANG, Yun-Shiang, LIU, Kuan-Fu, SU, Mao-Sen, KOU, Guang-Hsiung
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container_end_page 172
container_issue 3
container_start_page 165
container_title Diseases of aquatic organisms
container_volume 46
creator PENG, Shao-En
LO, Chu-Fang
LIN, San-Ching
CHEN, Li-Li
CHANG, Yun-Shiang
LIU, Kuan-Fu
SU, Mao-Sen
KOU, Guang-Hsiung
description In a survey of 27 Penaeus monodon culture ponds stocked with postlarvae (approximately PL10) at medium density (approximately 40 shrimp m(-2)), single-step nested white spot syndrome virus (WSSV) PCR was used to measure the WSSV infection rates in the shrimp populations within 1 mo after stocking. Seven ponds were initially WSSV-free, and the shrimp in 5 of these were harvested successfully. In the ponds (n = 6) where detection rates were higher than 50%, mass mortality occurred during the growth period, and none of these ponds was harvested successfully. In a subsequent study, P. monodon brooders were classified into 3 groups according to their WSSV infection status before and after spawning: brooders that were WSSV-positive before spawning were assigned to group A; spawners that became WSSV-positive only after spawning were assigned to group B; and group C consisted of brooders that were still WSSV-negative after spawning. WSSV screening showed that 75, 44 and 14%, respectively, of group A, B and C brooders produced nauplii that were WSSV-positive. Most (57%; 16/28) of the brooders in group A produced nauplii in which the WSSV prevalence was high (>50%). When a pond was stocked with high-prevalence nauplii from 1 of these group A brooders, an outbreak of white spot syndrome occurred within 3 wk and only approximately 20% of the initial population survived through to harvest (after 174 d). By contrast, 2 other ponds stocked with low-prevalence and WSSV-negative nauplii (derived respectively from 2 brooders in group B), both had much higher survival rates (70 to 80%) and yielded much larger (approximately 3x by weight) total harvests. We conclude that testing the nauplii is an effective and practical screening strategy for commercially cultured P. monodon.
doi_str_mv 10.3354/dao046165
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Seven ponds were initially WSSV-free, and the shrimp in 5 of these were harvested successfully. In the ponds (n = 6) where detection rates were higher than 50%, mass mortality occurred during the growth period, and none of these ponds was harvested successfully. In a subsequent study, P. monodon brooders were classified into 3 groups according to their WSSV infection status before and after spawning: brooders that were WSSV-positive before spawning were assigned to group A; spawners that became WSSV-positive only after spawning were assigned to group B; and group C consisted of brooders that were still WSSV-negative after spawning. WSSV screening showed that 75, 44 and 14%, respectively, of group A, B and C brooders produced nauplii that were WSSV-positive. Most (57%; 16/28) of the brooders in group A produced nauplii in which the WSSV prevalence was high (&gt;50%). When a pond was stocked with high-prevalence nauplii from 1 of these group A brooders, an outbreak of white spot syndrome occurred within 3 wk and only approximately 20% of the initial population survived through to harvest (after 174 d). By contrast, 2 other ponds stocked with low-prevalence and WSSV-negative nauplii (derived respectively from 2 brooders in group B), both had much higher survival rates (70 to 80%) and yielded much larger (approximately 3x by weight) total harvests. 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When a pond was stocked with high-prevalence nauplii from 1 of these group A brooders, an outbreak of white spot syndrome occurred within 3 wk and only approximately 20% of the initial population survived through to harvest (after 174 d). By contrast, 2 other ponds stocked with low-prevalence and WSSV-negative nauplii (derived respectively from 2 brooders in group B), both had much higher survival rates (70 to 80%) and yielded much larger (approximately 3x by weight) total harvests. 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When a pond was stocked with high-prevalence nauplii from 1 of these group A brooders, an outbreak of white spot syndrome occurred within 3 wk and only approximately 20% of the initial population survived through to harvest (after 174 d). By contrast, 2 other ponds stocked with low-prevalence and WSSV-negative nauplii (derived respectively from 2 brooders in group B), both had much higher survival rates (70 to 80%) and yielded much larger (approximately 3x by weight) total harvests. We conclude that testing the nauplii is an effective and practical screening strategy for commercially cultured P. monodon.</abstract><cop>Oldendorf</cop><pub>Inter-Research</pub><pmid>11710550</pmid><doi>10.3354/dao046165</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record>
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source MEDLINE; Inter-Research; Free E-Journal (出版社公開部分のみ); Alma/SFX Local Collection
subjects Animal aquaculture
Animal productions
Animals
Aquaculture
Biological and medical sciences
Crustacea
Disease Outbreaks - veterinary
DNA Viruses - genetics
DNA Viruses - isolation & purification
DNA, Viral - analysis
Fundamental and applied biological sciences. Psychology
Invertebrate aquaculture
Larva
Oocytes - virology
Penaeidae - growth & development
Penaeidae - virology
Penaeus monodon
Polymerase Chain Reaction - veterinary
Prevalence
Reproducibility of Results
Reproduction
Sensitivity and Specificity
Survival Analysis
White spot syndrome virus
title Performance of WSSV-infected and WSSV-negative Penaeus monodon postlarvae in culture ponds
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