Differential changes of cAMP-dependent protein kinase activity and 3H-cAMP binding sites in rat hippocampus during maturation and aging
The cyclic AMP-dependent protein kinase (PKA) has been involved in the brain aging process and recent papers have reported age-associated changes in enzyme activity in rat brain. The present study was undertaken to assess simultaneously PKA activity and regulatory (R) subunit levels during maturatio...
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| Veröffentlicht in: | Neuroscience letters 2001-11, Vol.315 (1), p.89-92 |
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| creator | Karege, Félicien Lambercy, Carmen Schwald, Michèle Steimer, Thierry Cissé, Mbaye |
| description | The cyclic AMP-dependent protein kinase (PKA) has been involved in the brain aging process and recent papers have reported age-associated changes in enzyme activity in rat brain. The present study was undertaken to assess simultaneously PKA activity and regulatory (R) subunit levels during maturation and aging. Five cohorts of rats of different ages were used, namely pups of 1 week and 3 weeks old, mature rats (2 months), postmature rats (1 year) and old rats (2 years or more). PKA activity and
3H-cAMP binding sites were determined in cytosolic fractions of hippocampus. Results showed a low PKA activity in newborn rats which increased in mature and postmature rats and finally declined in old rats (ANOVA,
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| doi_str_mv | 10.1016/S0304-3940(01)02358-8 |
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3H-cAMP binding sites were determined in cytosolic fractions of hippocampus. Results showed a low PKA activity in newborn rats which increased in mature and postmature rats and finally declined in old rats (ANOVA,
P<0.001). The maximum binding sites (Bmax) of
3H-cAMP which measure the PKA R subunit levels were elevated in newborn rats and declined in mature and old rats (ANOVA;
P<0.001). It is suggested the changes in PKA R subunit levels reflect an adaptative role in maturing process, a role which is lost in aging phase.</description><identifier>ISSN: 0304-3940</identifier><identifier>EISSN: 1872-7972</identifier><identifier>DOI: 10.1016/S0304-3940(01)02358-8</identifier><identifier>PMID: 11711222</identifier><identifier>CODEN: NELED5</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>Aging ; Aging - metabolism ; Animals ; Animals, Newborn ; Binding Sites ; Biological and medical sciences ; Cyclic AMP - metabolism ; Cyclic AMP-dependent protein kinase (PKA) activity ; Cyclic AMP-Dependent Protein Kinases - metabolism ; Cytosol - metabolism ; Development. Senescence. Regeneration. Transplantation ; Electrophoresis, Agar Gel ; Fluorometry ; Fundamental and applied biological sciences. Psychology ; Hippocampus - enzymology ; Hippocampus - metabolism ; In Vitro Techniques ; Male ; Maturation ; PKA regulator subunit ; Radioligand Assay ; Rat hippocampus ; Rats ; Rats, Wistar ; Vertebrates: nervous system and sense organs</subject><ispartof>Neuroscience letters, 2001-11, Vol.315 (1), p.89-92</ispartof><rights>2001 Elsevier Science Ireland Ltd</rights><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2382-41223b1450a8671bd2587e7d088fbf93b75433a7d612fc01b37bab1ee0f15baf3</citedby><cites>FETCH-LOGICAL-c2382-41223b1450a8671bd2587e7d088fbf93b75433a7d612fc01b37bab1ee0f15baf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0304-3940(01)02358-8$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14130764$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11711222$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Karege, Félicien</creatorcontrib><creatorcontrib>Lambercy, Carmen</creatorcontrib><creatorcontrib>Schwald, Michèle</creatorcontrib><creatorcontrib>Steimer, Thierry</creatorcontrib><creatorcontrib>Cissé, Mbaye</creatorcontrib><title>Differential changes of cAMP-dependent protein kinase activity and 3H-cAMP binding sites in rat hippocampus during maturation and aging</title><title>Neuroscience letters</title><addtitle>Neurosci Lett</addtitle><description>The cyclic AMP-dependent protein kinase (PKA) has been involved in the brain aging process and recent papers have reported age-associated changes in enzyme activity in rat brain. The present study was undertaken to assess simultaneously PKA activity and regulatory (R) subunit levels during maturation and aging. Five cohorts of rats of different ages were used, namely pups of 1 week and 3 weeks old, mature rats (2 months), postmature rats (1 year) and old rats (2 years or more). PKA activity and
3H-cAMP binding sites were determined in cytosolic fractions of hippocampus. Results showed a low PKA activity in newborn rats which increased in mature and postmature rats and finally declined in old rats (ANOVA,
P<0.001). The maximum binding sites (Bmax) of
3H-cAMP which measure the PKA R subunit levels were elevated in newborn rats and declined in mature and old rats (ANOVA;
P<0.001). It is suggested the changes in PKA R subunit levels reflect an adaptative role in maturing process, a role which is lost in aging phase.</description><subject>Aging</subject><subject>Aging - metabolism</subject><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Binding Sites</subject><subject>Biological and medical sciences</subject><subject>Cyclic AMP - metabolism</subject><subject>Cyclic AMP-dependent protein kinase (PKA) activity</subject><subject>Cyclic AMP-Dependent Protein Kinases - metabolism</subject><subject>Cytosol - metabolism</subject><subject>Development. Senescence. Regeneration. Transplantation</subject><subject>Electrophoresis, Agar Gel</subject><subject>Fluorometry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hippocampus - enzymology</subject><subject>Hippocampus - metabolism</subject><subject>In Vitro Techniques</subject><subject>Male</subject><subject>Maturation</subject><subject>PKA regulator subunit</subject><subject>Radioligand Assay</subject><subject>Rat hippocampus</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0304-3940</issn><issn>1872-7972</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u1TAQhS0EopfCI4C8AcEi4LGTa98VqkpLkYpAAtaWf8a3hsQJdlKpT8Br4_sjumRhWfL5zsz4DCHPgb0FBut335hgbSM2LXvN4A3jolONekBWoCRv5Ebyh2T1DzkhT0r5yRjroGsfkxMACcA5X5E_H2IImDHN0fTU3Zi0xULHQN3Z56-NxwmTryKd8jhjTPRXTKYgNW6Ot3G-oyZ5Kq6aHU1tTD6mLS1xrjUqnM1Mb-I0jc4M01KoX_JOH8y8VCmOaW832_r4lDwKpi_47Hifkh-XF9_Pr5rrLx8_nZ9dN44LxZu2Ti0stB0zai3Bet4pidIzpYING2Fl1wphpF8DD46BFdIaC4gsQGdNEKfk1aFu_dDvBcush1gc9r1JOC5FS86lqqeC3QF0eSwlY9BTjoPJdxqY3m1A7zegd_FqBnq_Aa2q78WxwWIH9PeuY-QVeHkETHGmD9kkF8s914Jgct1W7v2BwxrHbcSsi4uYHPqY0c3aj_E_o_wFXlejUQ</recordid><startdate>20011123</startdate><enddate>20011123</enddate><creator>Karege, Félicien</creator><creator>Lambercy, Carmen</creator><creator>Schwald, Michèle</creator><creator>Steimer, Thierry</creator><creator>Cissé, Mbaye</creator><general>Elsevier Ireland Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20011123</creationdate><title>Differential changes of cAMP-dependent protein kinase activity and 3H-cAMP binding sites in rat hippocampus during maturation and aging</title><author>Karege, Félicien ; Lambercy, Carmen ; Schwald, Michèle ; Steimer, Thierry ; Cissé, Mbaye</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2382-41223b1450a8671bd2587e7d088fbf93b75433a7d612fc01b37bab1ee0f15baf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Aging</topic><topic>Aging - metabolism</topic><topic>Animals</topic><topic>Animals, Newborn</topic><topic>Binding Sites</topic><topic>Biological and medical sciences</topic><topic>Cyclic AMP - metabolism</topic><topic>Cyclic AMP-dependent protein kinase (PKA) activity</topic><topic>Cyclic AMP-Dependent Protein Kinases - metabolism</topic><topic>Cytosol - metabolism</topic><topic>Development. Senescence. Regeneration. Transplantation</topic><topic>Electrophoresis, Agar Gel</topic><topic>Fluorometry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hippocampus - enzymology</topic><topic>Hippocampus - metabolism</topic><topic>In Vitro Techniques</topic><topic>Male</topic><topic>Maturation</topic><topic>PKA regulator subunit</topic><topic>Radioligand Assay</topic><topic>Rat hippocampus</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Karege, Félicien</creatorcontrib><creatorcontrib>Lambercy, Carmen</creatorcontrib><creatorcontrib>Schwald, Michèle</creatorcontrib><creatorcontrib>Steimer, Thierry</creatorcontrib><creatorcontrib>Cissé, Mbaye</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Neuroscience letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Karege, Félicien</au><au>Lambercy, Carmen</au><au>Schwald, Michèle</au><au>Steimer, Thierry</au><au>Cissé, Mbaye</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differential changes of cAMP-dependent protein kinase activity and 3H-cAMP binding sites in rat hippocampus during maturation and aging</atitle><jtitle>Neuroscience letters</jtitle><addtitle>Neurosci Lett</addtitle><date>2001-11-23</date><risdate>2001</risdate><volume>315</volume><issue>1</issue><spage>89</spage><epage>92</epage><pages>89-92</pages><issn>0304-3940</issn><eissn>1872-7972</eissn><coden>NELED5</coden><abstract>The cyclic AMP-dependent protein kinase (PKA) has been involved in the brain aging process and recent papers have reported age-associated changes in enzyme activity in rat brain. The present study was undertaken to assess simultaneously PKA activity and regulatory (R) subunit levels during maturation and aging. Five cohorts of rats of different ages were used, namely pups of 1 week and 3 weeks old, mature rats (2 months), postmature rats (1 year) and old rats (2 years or more). PKA activity and
3H-cAMP binding sites were determined in cytosolic fractions of hippocampus. Results showed a low PKA activity in newborn rats which increased in mature and postmature rats and finally declined in old rats (ANOVA,
P<0.001). The maximum binding sites (Bmax) of
3H-cAMP which measure the PKA R subunit levels were elevated in newborn rats and declined in mature and old rats (ANOVA;
P<0.001). It is suggested the changes in PKA R subunit levels reflect an adaptative role in maturing process, a role which is lost in aging phase.</abstract><cop>Shannon</cop><pub>Elsevier Ireland Ltd</pub><pmid>11711222</pmid><doi>10.1016/S0304-3940(01)02358-8</doi><tpages>4</tpages></addata></record> |
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| subjects | Aging Aging - metabolism Animals Animals, Newborn Binding Sites Biological and medical sciences Cyclic AMP - metabolism Cyclic AMP-dependent protein kinase (PKA) activity Cyclic AMP-Dependent Protein Kinases - metabolism Cytosol - metabolism Development. Senescence. Regeneration. Transplantation Electrophoresis, Agar Gel Fluorometry Fundamental and applied biological sciences. Psychology Hippocampus - enzymology Hippocampus - metabolism In Vitro Techniques Male Maturation PKA regulator subunit Radioligand Assay Rat hippocampus Rats Rats, Wistar Vertebrates: nervous system and sense organs |
| title | Differential changes of cAMP-dependent protein kinase activity and 3H-cAMP binding sites in rat hippocampus during maturation and aging |
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