Direct methods and protein crystallography at low resolution

Direct methods and protein crystallography at low resolution

The tools of modern direct methods are examined and their limitations for solving protein structures discussed. Direct methods need atomic resolution data (1.1–1.2 Å) for structures of around 1000 atoms if no heavy atom is present. For low‐resolution data, alternative approaches are necessary and th...

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Veröffentlicht in:Acta crystallographica. Section D, Biological crystallography. Biological crystallography., 2000-10, Vol.56 (10), p.1205-1214
1. Verfasser: Gilmore, Christopher J.
Format: Artikel
Sprache:eng
Schlagworte:
Bacterial Proteins - chemistry
Crystallography, X-Ray - methods
direct methods
maximum entropy
Models, Theoretical
Porins - chemistry
Protein Conformation
Proteins - chemistry
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Zusammenfassung:The tools of modern direct methods are examined and their limitations for solving protein structures discussed. Direct methods need atomic resolution data (1.1–1.2 Å) for structures of around 1000 atoms if no heavy atom is present. For low‐resolution data, alternative approaches are necessary and these include maximum entropy, symbolic addition, Sayre's equation, group scattering factors and electron microscopy.
ISSN:1399-0047
0907-4449
1399-0047
DOI:10.1107/S0907444900009355