β-Glucosidase and peroxidase stability in crude enzyme extracts from green beans of Vanilla planifolia Andrews
The extraction method for β‐glucosidase from green vanilla beans has been studied. The effect of storage of green beans and protein extracts on β‐glucosidase and peroxidase activity was investigated: the best method, resulting in the highest enzyme activities, particularly for glucosidase, was throu...
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| Veröffentlicht in: | Phytochemical analysis 2001-05, Vol.12 (3), p.174-179 |
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| container_title | Phytochemical analysis |
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| creator | Dignum, Mark J. W. Kerler, Josef Verpoorte, Rob |
| description | The extraction method for β‐glucosidase from green vanilla beans has been studied. The effect of storage of green beans and protein extracts on β‐glucosidase and peroxidase activity was investigated: the best method, resulting in the highest enzyme activities, particularly for glucosidase, was through extraction of very fresh green beans in the presence of BisTris propane buffer at pH 8. The best method for storage of the extracts was at −80°C after addition of 15% glycerol, when over 90% of initial activity was still present. Peroxidase activity did not change in frozen beans or in frozen extracts. Copyright © 2001 John Wiley & Sons, Ltd. |
| doi_str_mv | 10.1002/pca.578 |
| format | Article |
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Copyright © 2001 John Wiley & Sons, Ltd.</description><identifier>ISSN: 0958-0344</identifier><identifier>EISSN: 1099-1565</identifier><identifier>DOI: 10.1002/pca.578</identifier><identifier>PMID: 11705022</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>beta-Glucosidase - metabolism ; Enzyme extraction ; enzyme stability ; Orchidaceae - enzymology ; peroxidase ; Peroxidases - metabolism ; phenylalanine ammonia lyase ; Plant Extracts - metabolism ; Vanilla planifolia ; β-glucosidase</subject><ispartof>Phytochemical analysis, 2001-05, Vol.12 (3), p.174-179</ispartof><rights>Copyright © 2001 John Wiley & Sons, Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3518-c930487bca2b56ef5f811bba0b183a3b92765abd8240bc277ce2aec7544b5a2c3</citedby><cites>FETCH-LOGICAL-c3518-c930487bca2b56ef5f811bba0b183a3b92765abd8240bc277ce2aec7544b5a2c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fpca.578$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fpca.578$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11705022$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dignum, Mark J. 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Copyright © 2001 John Wiley & Sons, Ltd.</description><subject>beta-Glucosidase - metabolism</subject><subject>Enzyme extraction</subject><subject>enzyme stability</subject><subject>Orchidaceae - enzymology</subject><subject>peroxidase</subject><subject>Peroxidases - metabolism</subject><subject>phenylalanine ammonia lyase</subject><subject>Plant Extracts - metabolism</subject><subject>Vanilla planifolia</subject><subject>β-glucosidase</subject><issn>0958-0344</issn><issn>1099-1565</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kMtu1DAUhi1ERacF8QbIK1igFF_iOFmORmWKNG1nAWVpHTsnyJAbdqLO8Fg8CM-EUUawYvWfo_Pp19FHyEvOrjhj4t3o4Erp8glZcVZVGVeFekpWrFJlxmSen5OLGL8ylm5V8Yycc66ZYkKsyPDrZ7ZtZzdEX0NECn1NRwzDYVnjBNa3fjpS31MX5hop9j-OXYrDFMBNkTZh6OiXgNhTi9BHOjT0AXrftkDHNg3N0Hqg674O-Bifk7MG2ogvTnlJPr2__ri5yXb32w-b9S5zUvEyc5VkeamtA2FVgY1qSs6tBWZ5KUHaSuhCga1LkTPrhNYOBaDTKs-tAuHkJXm99I5h-D5jnEzno8P0VI_DHI0WQquqkAl8s4AuDDEGbMwYfAfhaDgzf9ya5NYkt4l8daqcbYf1P-4kMwFvF-DRt3j8X4_Zb9ZLXbbQPk54-EtD-GYKLbUyn--2hpe3D1Lt9mYvfwMBaZPE</recordid><startdate>200105</startdate><enddate>200105</enddate><creator>Dignum, Mark J. 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W.</creatorcontrib><creatorcontrib>Kerler, Josef</creatorcontrib><creatorcontrib>Verpoorte, Rob</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Phytochemical analysis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dignum, Mark J. W.</au><au>Kerler, Josef</au><au>Verpoorte, Rob</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>β-Glucosidase and peroxidase stability in crude enzyme extracts from green beans of Vanilla planifolia Andrews</atitle><jtitle>Phytochemical analysis</jtitle><addtitle>Phytochem. Anal</addtitle><date>2001-05</date><risdate>2001</risdate><volume>12</volume><issue>3</issue><spage>174</spage><epage>179</epage><pages>174-179</pages><issn>0958-0344</issn><eissn>1099-1565</eissn><abstract>The extraction method for β‐glucosidase from green vanilla beans has been studied. The effect of storage of green beans and protein extracts on β‐glucosidase and peroxidase activity was investigated: the best method, resulting in the highest enzyme activities, particularly for glucosidase, was through extraction of very fresh green beans in the presence of BisTris propane buffer at pH 8. The best method for storage of the extracts was at −80°C after addition of 15% glycerol, when over 90% of initial activity was still present. Peroxidase activity did not change in frozen beans or in frozen extracts. Copyright © 2001 John Wiley & Sons, Ltd.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>11705022</pmid><doi>10.1002/pca.578</doi><tpages>6</tpages></addata></record> |
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| identifier | ISSN: 0958-0344 |
| ispartof | Phytochemical analysis, 2001-05, Vol.12 (3), p.174-179 |
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| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | beta-Glucosidase - metabolism Enzyme extraction enzyme stability Orchidaceae - enzymology peroxidase Peroxidases - metabolism phenylalanine ammonia lyase Plant Extracts - metabolism Vanilla planifolia β-glucosidase |
| title | β-Glucosidase and peroxidase stability in crude enzyme extracts from green beans of Vanilla planifolia Andrews |
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