Frequency of Apoptosis of Tumor-infiltrating Lymphocytes Induced by Fas Counterattack in Human Colorectal Carcinoma and Its Correlation with Prognosis
We investigated apoptosis in tumor-infiltrating lymphocytes (TILs) obtained from 41 colorectal carcinomas by in situ nick translation (ISNT). When the ISNT labeling index (LI) was determined as the number of positive nuclei per 1000 nuclei of TIL in tissue sections, the median LI was 12.0 (range, 2–...
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| Veröffentlicht in: | Clinical cancer research 2000-09, Vol.6 (9), p.3560-3564 |
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| creator | OKADA, Kazuya KOMUTA, Ko HASHIMOTO, Satoshi MATSUZAKI, Sumihiro KANEMATSU, Takashi KOJI, Takehiko |
| description | We investigated
apoptosis in tumor-infiltrating lymphocytes (TILs) obtained from 41
colorectal carcinomas by in situ nick translation
(ISNT). When the ISNT labeling index (LI) was determined as the number
of positive nuclei per 1000 nuclei of TIL in tissue sections, the
median LI was 12.0 (range, 2–30). The ISNT LI of colorectal carcinoma
with lymph node metastasis was higher than that of colorectal carcinoma
without metastasis. The cases with a high LI of ≥12.0 had a
significantly poorer prognosis than those with a low LI. We also
confirmed immunohistochemically that a part of the TILs
expressed Fas using the sections adjacent to what contained abundant
ISNT-positive TILs. Moreover, Fas ligand (FasL) expression was detected
on the cell surface as well as the cytoplasm of colorectal cancer cells
in 61% of cases. Apoptosis in TILs was consistently seen more
frequently in FasL-positive cases than in FasL-negative ones. These
findings indicate that the FasL expressed in colorectal carcinoma cells
may kill the Fas-positive immune effective TILs by means of a Fas-FasL
system termed Fas counterattack. This tumor immune evasion induced by
FasL may therefore affect the malignant potential of human colorectal
carcinoma. |
| format | Article |
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apoptosis in tumor-infiltrating lymphocytes (TILs) obtained from 41
colorectal carcinomas by in situ nick translation
(ISNT). When the ISNT labeling index (LI) was determined as the number
of positive nuclei per 1000 nuclei of TIL in tissue sections, the
median LI was 12.0 (range, 2–30). The ISNT LI of colorectal carcinoma
with lymph node metastasis was higher than that of colorectal carcinoma
without metastasis. The cases with a high LI of ≥12.0 had a
significantly poorer prognosis than those with a low LI. We also
confirmed immunohistochemically that a part of the TILs
expressed Fas using the sections adjacent to what contained abundant
ISNT-positive TILs. Moreover, Fas ligand (FasL) expression was detected
on the cell surface as well as the cytoplasm of colorectal cancer cells
in 61% of cases. Apoptosis in TILs was consistently seen more
frequently in FasL-positive cases than in FasL-negative ones. These
findings indicate that the FasL expressed in colorectal carcinoma cells
may kill the Fas-positive immune effective TILs by means of a Fas-FasL
system termed Fas counterattack. This tumor immune evasion induced by
FasL may therefore affect the malignant potential of human colorectal
carcinoma.</description><identifier>ISSN: 1078-0432</identifier><identifier>EISSN: 1557-3265</identifier><identifier>PMID: 10999744</identifier><language>eng</language><publisher>Philadelphia, PA: American Association for Cancer Research</publisher><subject>Adult ; Aged ; Aged, 80 and over ; Apoptosis - physiology ; Biological and medical sciences ; Colorectal Neoplasms - immunology ; Colorectal Neoplasms - pathology ; DNA Fragmentation ; Fas Ligand Protein ; fas Receptor - biosynthesis ; fas Receptor - immunology ; Female ; Gastroenterology. Liver. Pancreas. Abdomen ; Humans ; Immunohistochemistry ; Lymphocytes, Tumor-Infiltrating - cytology ; Lymphocytes, Tumor-Infiltrating - immunology ; Male ; Medical sciences ; Membrane Glycoproteins - biosynthesis ; Membrane Glycoproteins - immunology ; Middle Aged ; Prognosis ; Staining and Labeling - methods ; Stomach. Duodenum. Small intestine. Colon. Rectum. Anus ; Tumors</subject><ispartof>Clinical cancer research, 2000-09, Vol.6 (9), p.3560-3564</ispartof><rights>2000 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,782,786</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1503795$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10999744$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>OKADA, Kazuya</creatorcontrib><creatorcontrib>KOMUTA, Ko</creatorcontrib><creatorcontrib>HASHIMOTO, Satoshi</creatorcontrib><creatorcontrib>MATSUZAKI, Sumihiro</creatorcontrib><creatorcontrib>KANEMATSU, Takashi</creatorcontrib><creatorcontrib>KOJI, Takehiko</creatorcontrib><title>Frequency of Apoptosis of Tumor-infiltrating Lymphocytes Induced by Fas Counterattack in Human Colorectal Carcinoma and Its Correlation with Prognosis</title><title>Clinical cancer research</title><addtitle>Clin Cancer Res</addtitle><description>We investigated
apoptosis in tumor-infiltrating lymphocytes (TILs) obtained from 41
colorectal carcinomas by in situ nick translation
(ISNT). When the ISNT labeling index (LI) was determined as the number
of positive nuclei per 1000 nuclei of TIL in tissue sections, the
median LI was 12.0 (range, 2–30). The ISNT LI of colorectal carcinoma
with lymph node metastasis was higher than that of colorectal carcinoma
without metastasis. The cases with a high LI of ≥12.0 had a
significantly poorer prognosis than those with a low LI. We also
confirmed immunohistochemically that a part of the TILs
expressed Fas using the sections adjacent to what contained abundant
ISNT-positive TILs. Moreover, Fas ligand (FasL) expression was detected
on the cell surface as well as the cytoplasm of colorectal cancer cells
in 61% of cases. Apoptosis in TILs was consistently seen more
frequently in FasL-positive cases than in FasL-negative ones. These
findings indicate that the FasL expressed in colorectal carcinoma cells
may kill the Fas-positive immune effective TILs by means of a Fas-FasL
system termed Fas counterattack. This tumor immune evasion induced by
FasL may therefore affect the malignant potential of human colorectal
carcinoma.</description><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Apoptosis - physiology</subject><subject>Biological and medical sciences</subject><subject>Colorectal Neoplasms - immunology</subject><subject>Colorectal Neoplasms - pathology</subject><subject>DNA Fragmentation</subject><subject>Fas Ligand Protein</subject><subject>fas Receptor - biosynthesis</subject><subject>fas Receptor - immunology</subject><subject>Female</subject><subject>Gastroenterology. Liver. Pancreas. Abdomen</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Lymphocytes, Tumor-Infiltrating - cytology</subject><subject>Lymphocytes, Tumor-Infiltrating - immunology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Membrane Glycoproteins - biosynthesis</subject><subject>Membrane Glycoproteins - immunology</subject><subject>Middle Aged</subject><subject>Prognosis</subject><subject>Staining and Labeling - methods</subject><subject>Stomach. Duodenum. Small intestine. Colon. Rectum. Anus</subject><subject>Tumors</subject><issn>1078-0432</issn><issn>1557-3265</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpF0M1q3DAUBWBTGpo07SsULUqzMujXspZh6CQDA-0iXRtZvhqrtSVXkgl-kT5vNGRCV7q6fDqC8666IULImtFGvC8zlm2NOaPX1ceUfmNMOMH8Q3VNsFJKcn5T_dtH-LuCNxsKFt0vYckhuXS-PK1ziLXz1k056uz8CR23eRmD2TIkdPDDamBA_Yb2OqFdWH2G4rI2f5Dz6HGdtS_rKUQwWU9op6NxPswaaT-gQz6_iRGmEh08enZ5RD9jOPnz_5-qK6unBJ8v5231a__9afdYH388HHb3x3qkTZvrtm-4GGzLuSS9sor3VFHa2KaXjAoLllluyKAVcGs4Iy3vjaRAhsZIrDhht9W319wlhlJDyt3skoFp0h7CmjpJqRSMsgK_XODazzB0S3Szjlv31mQBXy9AJ6MnG7U3Lv13AjOpRGF3r2x0p_HZRehMgVB6SFD6GbumUx0TDWYva7eN9w</recordid><startdate>20000901</startdate><enddate>20000901</enddate><creator>OKADA, Kazuya</creator><creator>KOMUTA, Ko</creator><creator>HASHIMOTO, Satoshi</creator><creator>MATSUZAKI, Sumihiro</creator><creator>KANEMATSU, Takashi</creator><creator>KOJI, Takehiko</creator><general>American Association for Cancer Research</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20000901</creationdate><title>Frequency of Apoptosis of Tumor-infiltrating Lymphocytes Induced by Fas Counterattack in Human Colorectal Carcinoma and Its Correlation with Prognosis</title><author>OKADA, Kazuya ; KOMUTA, Ko ; HASHIMOTO, Satoshi ; MATSUZAKI, Sumihiro ; KANEMATSU, Takashi ; KOJI, Takehiko</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h268t-8b645df84471b9f94b29226f6b7325fef3f4c1da9e4fc43184bc72e1d6c709413</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Apoptosis - physiology</topic><topic>Biological and medical sciences</topic><topic>Colorectal Neoplasms - immunology</topic><topic>Colorectal Neoplasms - pathology</topic><topic>DNA Fragmentation</topic><topic>Fas Ligand Protein</topic><topic>fas Receptor - biosynthesis</topic><topic>fas Receptor - immunology</topic><topic>Female</topic><topic>Gastroenterology. Liver. Pancreas. Abdomen</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Lymphocytes, Tumor-Infiltrating - cytology</topic><topic>Lymphocytes, Tumor-Infiltrating - immunology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Membrane Glycoproteins - biosynthesis</topic><topic>Membrane Glycoproteins - immunology</topic><topic>Middle Aged</topic><topic>Prognosis</topic><topic>Staining and Labeling - methods</topic><topic>Stomach. Duodenum. Small intestine. Colon. Rectum. Anus</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>OKADA, Kazuya</creatorcontrib><creatorcontrib>KOMUTA, Ko</creatorcontrib><creatorcontrib>HASHIMOTO, Satoshi</creatorcontrib><creatorcontrib>MATSUZAKI, Sumihiro</creatorcontrib><creatorcontrib>KANEMATSU, Takashi</creatorcontrib><creatorcontrib>KOJI, Takehiko</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical cancer research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>OKADA, Kazuya</au><au>KOMUTA, Ko</au><au>HASHIMOTO, Satoshi</au><au>MATSUZAKI, Sumihiro</au><au>KANEMATSU, Takashi</au><au>KOJI, Takehiko</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Frequency of Apoptosis of Tumor-infiltrating Lymphocytes Induced by Fas Counterattack in Human Colorectal Carcinoma and Its Correlation with Prognosis</atitle><jtitle>Clinical cancer research</jtitle><addtitle>Clin Cancer Res</addtitle><date>2000-09-01</date><risdate>2000</risdate><volume>6</volume><issue>9</issue><spage>3560</spage><epage>3564</epage><pages>3560-3564</pages><issn>1078-0432</issn><eissn>1557-3265</eissn><abstract>We investigated
apoptosis in tumor-infiltrating lymphocytes (TILs) obtained from 41
colorectal carcinomas by in situ nick translation
(ISNT). When the ISNT labeling index (LI) was determined as the number
of positive nuclei per 1000 nuclei of TIL in tissue sections, the
median LI was 12.0 (range, 2–30). The ISNT LI of colorectal carcinoma
with lymph node metastasis was higher than that of colorectal carcinoma
without metastasis. The cases with a high LI of ≥12.0 had a
significantly poorer prognosis than those with a low LI. We also
confirmed immunohistochemically that a part of the TILs
expressed Fas using the sections adjacent to what contained abundant
ISNT-positive TILs. Moreover, Fas ligand (FasL) expression was detected
on the cell surface as well as the cytoplasm of colorectal cancer cells
in 61% of cases. Apoptosis in TILs was consistently seen more
frequently in FasL-positive cases than in FasL-negative ones. These
findings indicate that the FasL expressed in colorectal carcinoma cells
may kill the Fas-positive immune effective TILs by means of a Fas-FasL
system termed Fas counterattack. This tumor immune evasion induced by
FasL may therefore affect the malignant potential of human colorectal
carcinoma.</abstract><cop>Philadelphia, PA</cop><pub>American Association for Cancer Research</pub><pmid>10999744</pmid><tpages>5</tpages></addata></record> |
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| ispartof | Clinical cancer research, 2000-09, Vol.6 (9), p.3560-3564 |
| issn | 1078-0432 1557-3265 |
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| source | MEDLINE; American Association for Cancer Research; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Adult Aged Aged, 80 and over Apoptosis - physiology Biological and medical sciences Colorectal Neoplasms - immunology Colorectal Neoplasms - pathology DNA Fragmentation Fas Ligand Protein fas Receptor - biosynthesis fas Receptor - immunology Female Gastroenterology. Liver. Pancreas. Abdomen Humans Immunohistochemistry Lymphocytes, Tumor-Infiltrating - cytology Lymphocytes, Tumor-Infiltrating - immunology Male Medical sciences Membrane Glycoproteins - biosynthesis Membrane Glycoproteins - immunology Middle Aged Prognosis Staining and Labeling - methods Stomach. Duodenum. Small intestine. Colon. Rectum. Anus Tumors |
| title | Frequency of Apoptosis of Tumor-infiltrating Lymphocytes Induced by Fas Counterattack in Human Colorectal Carcinoma and Its Correlation with Prognosis |
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