Subtractive Gene Cloning Using Dynabeads Oligo(dT)25 for Elucidation of Pseudohyphal Formation in Candida tropicalis

The dimorphic transition from yeast to pseudohyphae in Candida tropicalis occurs following the addition of ethanol to a synthetic medium containing glucose. We developed a method of subtractive gene cloning to isolate genes, of which the expression was apparently specific for pseudohyphal formation...

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Veröffentlicht in:	Nihon Ishinkin Gakkai zasshi 2001/10/30, Vol.42(4), pp.243-251
Hauptverfasser:	Imanishi, Yumi, Kawai, Toshiko, Iwaguchi, Shin-Ichi, Suzuki, Takahito, Kamihara, Teijiro, Yokoyama, Koji, Nishimura, Kazuko
Format:	Artikel
Sprache:	eng ; jpn
Schlagworte:	Candida - genetics Candida - growth & development Candida tropicalis Cloning, Molecular - methods ethanol Gene Library Genes, Fungal homology search hyphal formation Iron Oligodeoxyribonucleotides Polymerase Chain Reaction RNA, Messenger subtractive cloning
Online-Zugang:	Volltext
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