Antioxidative Effect of Maillard Reaction Products Formed from Honey at Different Reaction Times

Maillard reaction products (MRP) were synthesized from honey-lysine by refluxing with water for 4, 8, 12, 16, and 20 h. The MRP from each reaction time were added to and reacted with a linoleic acid buffered emulsion at 37 °C. The MRP from each of the five reaction times were added to the linoleic a...

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Veröffentlicht in:Journal of agricultural and food chemistry 2000-09, Vol.48 (9), p.3985-3989
Hauptverfasser: Antony, S. M, Han, I. Y, Rieck, J. R, Dawson, P. L
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container_end_page 3989
container_issue 9
container_start_page 3985
container_title Journal of agricultural and food chemistry
container_volume 48
creator Antony, S. M
Han, I. Y
Rieck, J. R
Dawson, P. L
description Maillard reaction products (MRP) were synthesized from honey-lysine by refluxing with water for 4, 8, 12, 16, and 20 h. The MRP from each reaction time were added to and reacted with a linoleic acid buffered emulsion at 37 °C. The MRP from each of the five reaction times were added to the linoleic acid emulsion (LAE) at 1%, 5%, 10%, 15%, and 20% (v/v). The antioxidative effect of MRP on the LAE was determined spectrophotometrically at 234 nm. The MRP pH and brown pigment formation (450 nm) was measured. Absorbance at 450 nm increased from 0.6 to 1.6 between the 4 h and 20 h MRP treatments, respectively. The pH of the MRP decreased from 4 h to 20 h reaction solutions, ranging from 4.2 to 3.65. The antioxidative effect increased at each reaction time increment. Within each reaction time, the antioxidative effect was maximized between 10% and 15% addition levels. Keywords: Maillard reaction products; antioxidants; linoleic acid; honey
doi_str_mv 10.1021/jf000305x
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M</creatorcontrib><creatorcontrib>Han, I. Y</creatorcontrib><creatorcontrib>Rieck, J. R</creatorcontrib><creatorcontrib>Dawson, P. L</creatorcontrib><title>Antioxidative Effect of Maillard Reaction Products Formed from Honey at Different Reaction Times</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>Maillard reaction products (MRP) were synthesized from honey-lysine by refluxing with water for 4, 8, 12, 16, and 20 h. The MRP from each reaction time were added to and reacted with a linoleic acid buffered emulsion at 37 °C. The MRP from each of the five reaction times were added to the linoleic acid emulsion (LAE) at 1%, 5%, 10%, 15%, and 20% (v/v). The antioxidative effect of MRP on the LAE was determined spectrophotometrically at 234 nm. The MRP pH and brown pigment formation (450 nm) was measured. Absorbance at 450 nm increased from 0.6 to 1.6 between the 4 h and 20 h MRP treatments, respectively. 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Psychology</subject><subject>Gas Chromatography-Mass Spectrometry</subject><subject>General aspects</subject><subject>Honey - analysis</subject><subject>Linoleic Acid - chemistry</subject><subject>Lysine - chemistry</subject><subject>Maillard Reaction</subject><subject>Miscellaneous</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpt0FFrFDEQB_AgFntWH_oFJCAWfNiabDab7GNpe7Zw0mLP53SSTSDn7aYm2XL99kb2OH3wKTDzmyHzR-iUknNKavpl4wghjPDdK7SgvCYVp1S-RgtSmpXkLT1Gb1PaFCS5IG_QMSVdxxmhC_R4MWYfdr6H7J8tvnbOmoyDw9_Ab7cQe_zdgilkxPcx9JPJCS9DHGyPXQwDvgmjfcGQ8ZUvo9GO-e_A2g82vUNHDrbJvt-_J-jH8np9eVOt7r7eXl6sKmBC5Mo4cJ3rWUOYkIbWnGvSaFcqToNlrbauB0lo34E2bae11U2ne4ByXiedZCfobN77FMOvyaasBp-MLTeMNkxJiboWlIq2wM8zNDGkFK1TT9EPEF8UJepPnOoQZ7Ef9ksnXU7-R875FfBxDyAZ2LoIo_Hp4CRtGymKqmblU7a7QxfiT9UKJrha3z-oq2b1ULMlV6viP80eTFKbMMWxJPef7_0G2oCZCg</recordid><startdate>20000901</startdate><enddate>20000901</enddate><creator>Antony, S. 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Food Chem</addtitle><date>2000-09-01</date><risdate>2000</risdate><volume>48</volume><issue>9</issue><spage>3985</spage><epage>3989</epage><pages>3985-3989</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><coden>JAFCAU</coden><abstract>Maillard reaction products (MRP) were synthesized from honey-lysine by refluxing with water for 4, 8, 12, 16, and 20 h. The MRP from each reaction time were added to and reacted with a linoleic acid buffered emulsion at 37 °C. The MRP from each of the five reaction times were added to the linoleic acid emulsion (LAE) at 1%, 5%, 10%, 15%, and 20% (v/v). The antioxidative effect of MRP on the LAE was determined spectrophotometrically at 234 nm. The MRP pH and brown pigment formation (450 nm) was measured. Absorbance at 450 nm increased from 0.6 to 1.6 between the 4 h and 20 h MRP treatments, respectively. The pH of the MRP decreased from 4 h to 20 h reaction solutions, ranging from 4.2 to 3.65. The antioxidative effect increased at each reaction time increment. Within each reaction time, the antioxidative effect was maximized between 10% and 15% addition levels. Keywords: Maillard reaction products; antioxidants; linoleic acid; honey</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>10995301</pmid><doi>10.1021/jf000305x</doi><tpages>5</tpages></addata></record>
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source MEDLINE; American Chemical Society Journals
subjects Antioxidants - chemistry
Biological and medical sciences
Food industries
Fundamental and applied biological sciences. Psychology
Gas Chromatography-Mass Spectrometry
General aspects
Honey - analysis
Linoleic Acid - chemistry
Lysine - chemistry
Maillard Reaction
Miscellaneous
title Antioxidative Effect of Maillard Reaction Products Formed from Honey at Different Reaction Times
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