Uptake of 99mTc-MIBI and 99mTc-Tetrofosmin into Malignant Versus Nonmalignant Breast Cell Lines
The kinetics and cellular uptake of 99mTc-2-hexakis 2-methoxyiso-butyl-isonitrile (MIBI) and 99mTc-1 ,2-bis[bis(2-ethoxyethyl)phosphino]ethane (tetrofosmin) into malignant versus nonmalignant human breast cell lines were investigated and compared. At specific intervals after incubation at 37 degrees...
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| Veröffentlicht in: | The Journal of nuclear medicine (1978) 2000-09, Vol.41 (9), p.1495-1499 |
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| container_title | The Journal of nuclear medicine (1978) |
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| creator | Rodrigues, Margarida Chehne, Fadi Kalinowska, Waclawa Berghammer, Peter Zielinski, Christoph Sinzinger, Helmut |
| description | The kinetics and cellular uptake of 99mTc-2-hexakis 2-methoxyiso-butyl-isonitrile (MIBI) and 99mTc-1 ,2-bis[bis(2-ethoxyethyl)phosphino]ethane (tetrofosmin) into malignant versus nonmalignant human breast cell lines were investigated and compared.
At specific intervals after incubation at 37 degrees C and 22 degrees C with 99mTc-MIBI or 99mTc-tetrofosmin, the uptake characteristics of radiotracers into human adenocarcinoma breast cell lines MCF-7 and SK-BR-3 and human breast, nontumor cell line HBL-100 were assessed.
The uptake of 99mTc-MIBI and 99mTc-tetrofosmin was lower at an incubation temperature of 22 degrees C than that at 37 degrees C in the 3 cell lines. In MCF-7 and in SK-BR-3 cells the uptake of 99mTc-MIBI was significantly higher than the uptake of 99mTc-tetrofosmin. The uptake of 99mTc-MIBI was significantly higher into MCF-7 and SK-BR-3 cells than that into HBL-100 cells. In comparison with HBL-100 cells, uptake of 99mTc-tetrofosmin into SK-BR-3 cells was significantly higher, whereas uptake into MCF-7 cells was similar.
In vitro data suggest that 99mTc-MIBI may be a better tracer than 99mTc-tetrofosmin for discrimination between malignant and nonmalignant breast disease. |
| format | Article |
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At specific intervals after incubation at 37 degrees C and 22 degrees C with 99mTc-MIBI or 99mTc-tetrofosmin, the uptake characteristics of radiotracers into human adenocarcinoma breast cell lines MCF-7 and SK-BR-3 and human breast, nontumor cell line HBL-100 were assessed.
The uptake of 99mTc-MIBI and 99mTc-tetrofosmin was lower at an incubation temperature of 22 degrees C than that at 37 degrees C in the 3 cell lines. In MCF-7 and in SK-BR-3 cells the uptake of 99mTc-MIBI was significantly higher than the uptake of 99mTc-tetrofosmin. The uptake of 99mTc-MIBI was significantly higher into MCF-7 and SK-BR-3 cells than that into HBL-100 cells. In comparison with HBL-100 cells, uptake of 99mTc-tetrofosmin into SK-BR-3 cells was significantly higher, whereas uptake into MCF-7 cells was similar.
In vitro data suggest that 99mTc-MIBI may be a better tracer than 99mTc-tetrofosmin for discrimination between malignant and nonmalignant breast disease.</description><identifier>ISSN: 0161-5505</identifier><identifier>EISSN: 1535-5667</identifier><identifier>PMID: 10994728</identifier><language>eng</language><publisher>Reston, VA: Soc Nuclear Med</publisher><subject>Adenocarcinoma ; Biological and medical sciences ; Biological Transport ; Breast ; Breast Neoplasms ; Cell Line ; Endocrine glands. Genital system. Mammary gland ; Female ; Gynecology. Andrology. Obstetrics ; Humans ; Investigative techniques, diagnostic techniques (general aspects) ; Kinetics ; Mammary gland diseases ; Medical sciences ; Organophosphorus Compounds - pharmacokinetics ; Organotechnetium Compounds - pharmacokinetics ; Radionuclide investigations ; Radiopharmaceuticals - pharmacokinetics ; Technetium Tc 99m Sestamibi - pharmacokinetics ; Tumor Cells, Cultured ; Tumors</subject><ispartof>The Journal of nuclear medicine (1978), 2000-09, Vol.41 (9), p.1495-1499</ispartof><rights>2000 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1502446$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10994728$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rodrigues, Margarida</creatorcontrib><creatorcontrib>Chehne, Fadi</creatorcontrib><creatorcontrib>Kalinowska, Waclawa</creatorcontrib><creatorcontrib>Berghammer, Peter</creatorcontrib><creatorcontrib>Zielinski, Christoph</creatorcontrib><creatorcontrib>Sinzinger, Helmut</creatorcontrib><title>Uptake of 99mTc-MIBI and 99mTc-Tetrofosmin into Malignant Versus Nonmalignant Breast Cell Lines</title><title>The Journal of nuclear medicine (1978)</title><addtitle>J Nucl Med</addtitle><description>The kinetics and cellular uptake of 99mTc-2-hexakis 2-methoxyiso-butyl-isonitrile (MIBI) and 99mTc-1 ,2-bis[bis(2-ethoxyethyl)phosphino]ethane (tetrofosmin) into malignant versus nonmalignant human breast cell lines were investigated and compared.
At specific intervals after incubation at 37 degrees C and 22 degrees C with 99mTc-MIBI or 99mTc-tetrofosmin, the uptake characteristics of radiotracers into human adenocarcinoma breast cell lines MCF-7 and SK-BR-3 and human breast, nontumor cell line HBL-100 were assessed.
The uptake of 99mTc-MIBI and 99mTc-tetrofosmin was lower at an incubation temperature of 22 degrees C than that at 37 degrees C in the 3 cell lines. In MCF-7 and in SK-BR-3 cells the uptake of 99mTc-MIBI was significantly higher than the uptake of 99mTc-tetrofosmin. The uptake of 99mTc-MIBI was significantly higher into MCF-7 and SK-BR-3 cells than that into HBL-100 cells. In comparison with HBL-100 cells, uptake of 99mTc-tetrofosmin into SK-BR-3 cells was significantly higher, whereas uptake into MCF-7 cells was similar.
In vitro data suggest that 99mTc-MIBI may be a better tracer than 99mTc-tetrofosmin for discrimination between malignant and nonmalignant breast disease.</description><subject>Adenocarcinoma</subject><subject>Biological and medical sciences</subject><subject>Biological Transport</subject><subject>Breast</subject><subject>Breast Neoplasms</subject><subject>Cell Line</subject><subject>Endocrine glands. Genital system. Mammary gland</subject><subject>Female</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Humans</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Kinetics</subject><subject>Mammary gland diseases</subject><subject>Medical sciences</subject><subject>Organophosphorus Compounds - pharmacokinetics</subject><subject>Organotechnetium Compounds - pharmacokinetics</subject><subject>Radionuclide investigations</subject><subject>Radiopharmaceuticals - pharmacokinetics</subject><subject>Technetium Tc 99m Sestamibi - pharmacokinetics</subject><subject>Tumor Cells, Cultured</subject><subject>Tumors</subject><issn>0161-5505</issn><issn>1535-5667</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkEtLw0AUhYMotlb_gsxCdBWYybwySy0-Cq1uWrfhJrlppyaTOpMg_ntTWnV1OYePj8s5icZMchlLpfRpNKZMsVhKKkfRRQhbSqlK0_Q8GjFqjNBJOo6y1a6DDyRtRYxplkW8mD3MCLjyGJfY-bZqQ2Mdsa5ryQJqu3bgOvKOPvSBvLau-esePELoyBTrmsytw3AZnVVQB7w63km0enpcTl_i-dvzbHo_jzeMyTTmWAqFSuYlN5UxNK-gZBxyhghaa-C0VJUEHDrBkeZagUhRmyIvK50Umk-i24N359vPHkOXNTYUwxvgsO1DppNEs2GPAbw-gn3eYJntvG3Af2e_kwzAzRGAUEBdeXCFDf-cpIkQe8_dAdvY9ebLesxcX9QIfi_dukawzGRMGMl_AN-beCw</recordid><startdate>200009</startdate><enddate>200009</enddate><creator>Rodrigues, Margarida</creator><creator>Chehne, Fadi</creator><creator>Kalinowska, Waclawa</creator><creator>Berghammer, Peter</creator><creator>Zielinski, Christoph</creator><creator>Sinzinger, Helmut</creator><general>Soc Nuclear Med</general><general>Society of Nuclear Medicine</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>200009</creationdate><title>Uptake of 99mTc-MIBI and 99mTc-Tetrofosmin into Malignant Versus Nonmalignant Breast Cell Lines</title><author>Rodrigues, Margarida ; Chehne, Fadi ; Kalinowska, Waclawa ; Berghammer, Peter ; Zielinski, Christoph ; Sinzinger, Helmut</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h1158-3ed46e65bd39f990bfad13ab1eea777a30d6f5ae13a43e0b76a48e79cbdf72c73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Adenocarcinoma</topic><topic>Biological and medical sciences</topic><topic>Biological Transport</topic><topic>Breast</topic><topic>Breast Neoplasms</topic><topic>Cell Line</topic><topic>Endocrine glands. Genital system. Mammary gland</topic><topic>Female</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Humans</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Kinetics</topic><topic>Mammary gland diseases</topic><topic>Medical sciences</topic><topic>Organophosphorus Compounds - pharmacokinetics</topic><topic>Organotechnetium Compounds - pharmacokinetics</topic><topic>Radionuclide investigations</topic><topic>Radiopharmaceuticals - pharmacokinetics</topic><topic>Technetium Tc 99m Sestamibi - pharmacokinetics</topic><topic>Tumor Cells, Cultured</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rodrigues, Margarida</creatorcontrib><creatorcontrib>Chehne, Fadi</creatorcontrib><creatorcontrib>Kalinowska, Waclawa</creatorcontrib><creatorcontrib>Berghammer, Peter</creatorcontrib><creatorcontrib>Zielinski, Christoph</creatorcontrib><creatorcontrib>Sinzinger, Helmut</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of nuclear medicine (1978)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rodrigues, Margarida</au><au>Chehne, Fadi</au><au>Kalinowska, Waclawa</au><au>Berghammer, Peter</au><au>Zielinski, Christoph</au><au>Sinzinger, Helmut</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Uptake of 99mTc-MIBI and 99mTc-Tetrofosmin into Malignant Versus Nonmalignant Breast Cell Lines</atitle><jtitle>The Journal of nuclear medicine (1978)</jtitle><addtitle>J Nucl Med</addtitle><date>2000-09</date><risdate>2000</risdate><volume>41</volume><issue>9</issue><spage>1495</spage><epage>1499</epage><pages>1495-1499</pages><issn>0161-5505</issn><eissn>1535-5667</eissn><abstract>The kinetics and cellular uptake of 99mTc-2-hexakis 2-methoxyiso-butyl-isonitrile (MIBI) and 99mTc-1 ,2-bis[bis(2-ethoxyethyl)phosphino]ethane (tetrofosmin) into malignant versus nonmalignant human breast cell lines were investigated and compared.
At specific intervals after incubation at 37 degrees C and 22 degrees C with 99mTc-MIBI or 99mTc-tetrofosmin, the uptake characteristics of radiotracers into human adenocarcinoma breast cell lines MCF-7 and SK-BR-3 and human breast, nontumor cell line HBL-100 were assessed.
The uptake of 99mTc-MIBI and 99mTc-tetrofosmin was lower at an incubation temperature of 22 degrees C than that at 37 degrees C in the 3 cell lines. In MCF-7 and in SK-BR-3 cells the uptake of 99mTc-MIBI was significantly higher than the uptake of 99mTc-tetrofosmin. The uptake of 99mTc-MIBI was significantly higher into MCF-7 and SK-BR-3 cells than that into HBL-100 cells. In comparison with HBL-100 cells, uptake of 99mTc-tetrofosmin into SK-BR-3 cells was significantly higher, whereas uptake into MCF-7 cells was similar.
In vitro data suggest that 99mTc-MIBI may be a better tracer than 99mTc-tetrofosmin for discrimination between malignant and nonmalignant breast disease.</abstract><cop>Reston, VA</cop><pub>Soc Nuclear Med</pub><pmid>10994728</pmid><tpages>5</tpages></addata></record> |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | Adenocarcinoma Biological and medical sciences Biological Transport Breast Breast Neoplasms Cell Line Endocrine glands. Genital system. Mammary gland Female Gynecology. Andrology. Obstetrics Humans Investigative techniques, diagnostic techniques (general aspects) Kinetics Mammary gland diseases Medical sciences Organophosphorus Compounds - pharmacokinetics Organotechnetium Compounds - pharmacokinetics Radionuclide investigations Radiopharmaceuticals - pharmacokinetics Technetium Tc 99m Sestamibi - pharmacokinetics Tumor Cells, Cultured Tumors |
| title | Uptake of 99mTc-MIBI and 99mTc-Tetrofosmin into Malignant Versus Nonmalignant Breast Cell Lines |
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