c-src Tyrosine Kinase Is Associated with the Asialoglycoprotein Receptor in Human Hepatoma Cells
The asialoglycoprotein (ASGP) receptor is expressed on hepatocytes and liver-derived cell lines and is responsible for the endocytosis of galactose-terminal glycoproteins via the coated pit pathway. Prior data showed that tyrosine kinase activity plays an important role in this endocytic process, th...
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| Veröffentlicht in: | Molecular cell biology research communications 2001-11, Vol.4 (6), p.331-336 |
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| container_title | Molecular cell biology research communications |
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| creator | Parker, Amy Fallon, Robert J. |
| description | The asialoglycoprotein (ASGP) receptor is expressed on hepatocytes and liver-derived cell lines and is responsible for the endocytosis of galactose-terminal glycoproteins via the coated pit pathway. Prior data showed that tyrosine kinase activity plays an important role in this endocytic process, though the critical kinase(s) responsible for this effect are unknown. We have detected a 60-kDa protein which coprecipitates with ASGP receptor in detergent-solubilized lysates of HepG2 cells. This protein autophosphorylates and binds radioactive ATP. It comigrates with authentic pp60 c-src and is recognized by a specific anti-src monoclonal antibody. The kinase associated with the ASGP receptor retains the ability to phosphorylate exogenous substrates on tyrosine. In conclusion, the tyrosine kinase c-src associates with the ASGP receptor, a protein of the coated pit pathway of endocytosis. |
| doi_str_mv | 10.1006/mcbr.2001.0299 |
| format | Article |
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Prior data showed that tyrosine kinase activity plays an important role in this endocytic process, though the critical kinase(s) responsible for this effect are unknown. We have detected a 60-kDa protein which coprecipitates with ASGP receptor in detergent-solubilized lysates of HepG2 cells. This protein autophosphorylates and binds radioactive ATP. It comigrates with authentic pp60 c-src and is recognized by a specific anti-src monoclonal antibody. The kinase associated with the ASGP receptor retains the ability to phosphorylate exogenous substrates on tyrosine. In conclusion, the tyrosine kinase c-src associates with the ASGP receptor, a protein of the coated pit pathway of endocytosis.</description><identifier>ISSN: 1522-4724</identifier><identifier>EISSN: 1522-4732</identifier><identifier>DOI: 10.1006/mcbr.2001.0299</identifier><identifier>PMID: 11703091</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adenosine Triphosphate - metabolism ; Asialoglycoprotein Receptor ; Blotting, Western ; Carcinoma, Hepatocellular - metabolism ; Cell Line ; Electrophoresis, Polyacrylamide Gel ; Endocytosis ; Humans ; Immunoblotting ; Liver - metabolism ; Phosphorylation ; Precipitin Tests ; Protein Binding ; Protein-Tyrosine Kinases - metabolism ; Proto-Oncogene Proteins pp60(c-src) - metabolism ; Receptors, Cell Surface - metabolism ; src-Family Kinases ; Tumor Cells, Cultured</subject><ispartof>Molecular cell biology research communications, 2001-11, Vol.4 (6), p.331-336</ispartof><rights>2001 Academic Press</rights><rights>Copyright 2001 Academic Press.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c255t-ba569a3128fe8fa86c43845e05b90ef779e8c9cad8936297918cb4296d210e633</citedby><cites>FETCH-LOGICAL-c255t-ba569a3128fe8fa86c43845e05b90ef779e8c9cad8936297918cb4296d210e633</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11703091$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Parker, Amy</creatorcontrib><creatorcontrib>Fallon, Robert J.</creatorcontrib><title>c-src Tyrosine Kinase Is Associated with the Asialoglycoprotein Receptor in Human Hepatoma Cells</title><title>Molecular cell biology research communications</title><addtitle>Mol Cell Biol Res Commun</addtitle><description>The asialoglycoprotein (ASGP) receptor is expressed on hepatocytes and liver-derived cell lines and is responsible for the endocytosis of galactose-terminal glycoproteins via the coated pit pathway. Prior data showed that tyrosine kinase activity plays an important role in this endocytic process, though the critical kinase(s) responsible for this effect are unknown. We have detected a 60-kDa protein which coprecipitates with ASGP receptor in detergent-solubilized lysates of HepG2 cells. This protein autophosphorylates and binds radioactive ATP. It comigrates with authentic pp60 c-src and is recognized by a specific anti-src monoclonal antibody. The kinase associated with the ASGP receptor retains the ability to phosphorylate exogenous substrates on tyrosine. In conclusion, the tyrosine kinase c-src associates with the ASGP receptor, a protein of the coated pit pathway of endocytosis.</description><subject>Adenosine Triphosphate - metabolism</subject><subject>Asialoglycoprotein Receptor</subject><subject>Blotting, Western</subject><subject>Carcinoma, Hepatocellular - metabolism</subject><subject>Cell Line</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Endocytosis</subject><subject>Humans</subject><subject>Immunoblotting</subject><subject>Liver - metabolism</subject><subject>Phosphorylation</subject><subject>Precipitin Tests</subject><subject>Protein Binding</subject><subject>Protein-Tyrosine Kinases - metabolism</subject><subject>Proto-Oncogene Proteins pp60(c-src) - metabolism</subject><subject>Receptors, Cell Surface - metabolism</subject><subject>src-Family Kinases</subject><subject>Tumor Cells, Cultured</subject><issn>1522-4724</issn><issn>1522-4732</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kE1LAzEQhoMotlavHiUnb1uT7FdyLEVtsSBIPcdsdtZGdjc1SZX-e7O06MnLZAjPvMw8CF1TMqWEFHedrtyUEUKnhAlxgsY0ZyzJypSd_vYsG6EL7z9IHKC0PEejWElKBB2jN514p_F676w3PeAn0ysPeOnxzHurjQpQ428TNjhsIP4Z1dr3dq_t1tkApscvoGEbrMOxX-w6FStsVbCdwnNoW3-JzhrVerg6vhP0-nC_ni-S1fPjcj5bJZrleUgqlRdCpZTxBnijeKGzlGc5kLwSBJqyFMC10KrmIi2YKAXlusqYKGpGCRRpOkG3h9y42OcOfJCd8TpuoHqwOy9LxgoheBnB6QHU8WTvoJFbZzrl9pISOTiVg1M5OJWD0zhwc0zeVR3Uf_hRYgT4AYB435cBJ7020GuojQMdZG3Nf9k_U9aFlw</recordid><startdate>200111</startdate><enddate>200111</enddate><creator>Parker, Amy</creator><creator>Fallon, Robert J.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200111</creationdate><title>c-src Tyrosine Kinase Is Associated with the Asialoglycoprotein Receptor in Human Hepatoma Cells</title><author>Parker, Amy ; Fallon, Robert J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c255t-ba569a3128fe8fa86c43845e05b90ef779e8c9cad8936297918cb4296d210e633</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Adenosine Triphosphate - metabolism</topic><topic>Asialoglycoprotein Receptor</topic><topic>Blotting, Western</topic><topic>Carcinoma, Hepatocellular - metabolism</topic><topic>Cell Line</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Endocytosis</topic><topic>Humans</topic><topic>Immunoblotting</topic><topic>Liver - metabolism</topic><topic>Phosphorylation</topic><topic>Precipitin Tests</topic><topic>Protein Binding</topic><topic>Protein-Tyrosine Kinases - metabolism</topic><topic>Proto-Oncogene Proteins pp60(c-src) - metabolism</topic><topic>Receptors, Cell Surface - metabolism</topic><topic>src-Family Kinases</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Parker, Amy</creatorcontrib><creatorcontrib>Fallon, Robert J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular cell biology research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Parker, Amy</au><au>Fallon, Robert J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>c-src Tyrosine Kinase Is Associated with the Asialoglycoprotein Receptor in Human Hepatoma Cells</atitle><jtitle>Molecular cell biology research communications</jtitle><addtitle>Mol Cell Biol Res Commun</addtitle><date>2001-11</date><risdate>2001</risdate><volume>4</volume><issue>6</issue><spage>331</spage><epage>336</epage><pages>331-336</pages><issn>1522-4724</issn><eissn>1522-4732</eissn><abstract>The asialoglycoprotein (ASGP) receptor is expressed on hepatocytes and liver-derived cell lines and is responsible for the endocytosis of galactose-terminal glycoproteins via the coated pit pathway. Prior data showed that tyrosine kinase activity plays an important role in this endocytic process, though the critical kinase(s) responsible for this effect are unknown. We have detected a 60-kDa protein which coprecipitates with ASGP receptor in detergent-solubilized lysates of HepG2 cells. This protein autophosphorylates and binds radioactive ATP. It comigrates with authentic pp60 c-src and is recognized by a specific anti-src monoclonal antibody. The kinase associated with the ASGP receptor retains the ability to phosphorylate exogenous substrates on tyrosine. In conclusion, the tyrosine kinase c-src associates with the ASGP receptor, a protein of the coated pit pathway of endocytosis.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>11703091</pmid><doi>10.1006/mcbr.2001.0299</doi><tpages>6</tpages></addata></record> |
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| subjects | Adenosine Triphosphate - metabolism Asialoglycoprotein Receptor Blotting, Western Carcinoma, Hepatocellular - metabolism Cell Line Electrophoresis, Polyacrylamide Gel Endocytosis Humans Immunoblotting Liver - metabolism Phosphorylation Precipitin Tests Protein Binding Protein-Tyrosine Kinases - metabolism Proto-Oncogene Proteins pp60(c-src) - metabolism Receptors, Cell Surface - metabolism src-Family Kinases Tumor Cells, Cultured |
| title | c-src Tyrosine Kinase Is Associated with the Asialoglycoprotein Receptor in Human Hepatoma Cells |
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