Single molecule detection of double-stranded DNA in poly(methylmethacrylate) and polycarbonate microfluidic devices

Single photon burst techniques were used to detect double‐stranded DNA molecules in poly(methylmethacrylate) (PMMA) and polycarbonate (PC) microfluidic devices. A confocal epi‐illumination detection system was constructed to monitor the fluorescence signature from single DNA molecules that were mult...

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Veröffentlicht in:	Electrophoresis 2001-10, Vol.22 (18), p.3939-3948
Hauptverfasser:	Wabuyele, Musundi B., Ford, Sean M., Stryjewski, Wieslaw, Barrow, James, Soper, Steven A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteriophage lambda - chemistry DNA, Viral - analysis Double-stranded DNA Electrophoresis, Capillary - instrumentation Equipment Design Fluorescent Dyes - analysis Fluorescent Dyes - chemistry Fluorometry - instrumentation Intercalating Agents - analysis Intercalating Agents - chemistry Lasers Microchemistry - instrumentation Microfluidic devices Molecular Weight Poly(methylmethacrylate) Polycarboxylate Cement - chemistry Polymethyl Methacrylate - chemistry Quinolines - analysis Quinolines - chemistry Rheology Sensitivity and Specificity Thiazoles - analysis Thiazoles - chemistry Time Factors
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