Yeast recombinase FLP functions effectively in human cells for construction of adenovirus vectors

Yeast recombinase FLP functions effectively in human cells for construction of adenovirus vectors

We have recently developed a high-efficiency method of constructing adenovirus vectors based on Cre-mediated recombination between two plasmids co-transfected into 293 cells. The simplicity and efficiency of this method should greatly expedite the construction of most recombinant vectors. However, t...

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Veröffentlicht in:BioTechniques 2000, Vol.29 (3), p.524-528
Hauptverfasser: Ng, P, Cummings, D T, Evelegh, C M, Graham, F L
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container_title BioTechniques
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creator Ng, P
Cummings, D T
Evelegh, C M
Graham, F L
description We have recently developed a high-efficiency method of constructing adenovirus vectors based on Cre-mediated recombination between two plasmids co-transfected into 293 cells. The simplicity and efficiency of this method should greatly expedite the construction of most recombinant vectors. However, this system would not be suitable for constructing vectors bearing loxP sites elsewhere in the genome because of undesirable Cre-mediated vector rearrangements. To address this, we have developed a similar system using FLP-mediated site-specific recombination for the construction of adenovirus vectors.
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title Yeast recombinase FLP functions effectively in human cells for construction of adenovirus vectors
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