The replicative capacity of rhesus macaque peripheral blood mononuclear cells for simian immunodeficiency virus in vitro is predictive of the rate of progression to AIDS in vivo

Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Room E1240 Biomedical Science Tower, Pittsburgh, PA 15261, USA 1 Tulane Regional Primate Research Center, 18703 Three Rivers Road, Covington, LA 70433, USA 2 Author for correspondence: Michael Murphey-Cor...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Journal of general virology 2000-10, Vol.81 (10), p.2441-2449
Hauptverfasser: Seman, Amy L, Pewen, William F, Fresh, Lynn F, Martin, Louis N, Murphey-Corb, Michael
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 2449
container_issue 10
container_start_page 2441
container_title Journal of general virology
container_volume 81
creator Seman, Amy L
Pewen, William F
Fresh, Lynn F
Martin, Louis N
Murphey-Corb, Michael
description Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Room E1240 Biomedical Science Tower, Pittsburgh, PA 15261, USA 1 Tulane Regional Primate Research Center, 18703 Three Rivers Road, Covington, LA 70433, USA 2 Author for correspondence: Michael Murphey-Corb. Fax +1 412 648 1448. e-mail mcorb{at}pitt.edu Survival of rhesus macaques ( Macaca mulatta ) experimentally infected with simian immunodeficiency virus (SIV) varies significantly from animal to animal. Some animals die within 2 months while others survive for more than 5 years, even when identical inocula are used. This diversity in survival creates a significant problem in the design of therapeutic and vaccine trials using the SIV–macaque model because the use of small numbers of animals may provide results that are misleading. Identifying an in vitro assay that could determine the survival of monkeys prior to infection would prove extremely useful for stratifying experimental groups. Analysis of the survival of a cohort of 59 control animals obtained from over a decade of vaccine and therapeutic trials has demonstrated that the ability of peripheral blood mononuclear cells (PBMC) from a naïve animal to produce virus in vitro was highly predictive of disease progression in vivo following experimental inoculation. Animals classified in vitro as high producers of virus progressed to disease significantly more rapidly than animals classified as either low ( P =0·002) or intermediate ( P =0·013) producers of virus. The hierarchy of high and low virus production was maintained in purified CD4 + T cell cultures, indicating that this phenotype is an intrinsic property of the CD4 + T cell itself. These findings should significantly aid in the design of vaccine and therapeutic trials using the SIV–macaque model. Furthermore, since these studies suggest that the rate of virus replication is controlled by innate characteristics of the individual, they provide new insight into the pathogenesis of AIDS.
doi_str_mv 10.1099/0022-1317-81-10-2441
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_72267696</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>17572028</sourcerecordid><originalsourceid>FETCH-LOGICAL-c480t-4188e51375128b05df49f9f5685d8258accd71dedefd05fec1f018dc75c53ac03</originalsourceid><addsrcrecordid>eNqFkc1u1TAQhS1ERS-FN0DIKyQWobYTJ86yKn-VKrFou7Z87fHNoCQOdnLRfSzeEKepUHesPLK-OXNmDiHvOPvEWdteMiZEwUveFIoXnBWiqvgLsuNVLQuRgZdk9w85J69T-skYryrZvCLnq0DZlmJH_tx3QCNMPVoz4xGoNZOxOJ9o8DR2kJZEB2PNrwXoBBGnDqLp6b4PwdEhjGFcbA8mUgt9n6gPkSYc0IwUh2EZgwOPFmG0J3rEmMVwzMUcA8VEpwgO7ePYPG1enZj5sZ5iOERICcNI50Cvbj7fbZ3H8IacedMnePv0XpCHr1_ur78Xtz--3Vxf3Ra2UmwuKq4USF42kgu1Z9L5qvWtl7WSTgmpjLWu4Q6yQcekB8s948rZRlpZGsvKC_Jh081e8vZp1gOmdUszQliSboSom7qt_wvyRjaCCZXBagNtDClF8HqKOJh40pzpNRK9BqbXwLTi6-eaaW57_6S_7Adwz5q2EDPwcQM6PHS_MYI-wDhgnrLHoPPZn4n9Bd9rr0I</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17572028</pqid></control><display><type>article</type><title>The replicative capacity of rhesus macaque peripheral blood mononuclear cells for simian immunodeficiency virus in vitro is predictive of the rate of progression to AIDS in vivo</title><source>MEDLINE</source><source>Microbiology Society</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>Seman, Amy L ; Pewen, William F ; Fresh, Lynn F ; Martin, Louis N ; Murphey-Corb, Michael</creator><creatorcontrib>Seman, Amy L ; Pewen, William F ; Fresh, Lynn F ; Martin, Louis N ; Murphey-Corb, Michael</creatorcontrib><description>Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Room E1240 Biomedical Science Tower, Pittsburgh, PA 15261, USA 1 Tulane Regional Primate Research Center, 18703 Three Rivers Road, Covington, LA 70433, USA 2 Author for correspondence: Michael Murphey-Corb. Fax +1 412 648 1448. e-mail mcorb{at}pitt.edu Survival of rhesus macaques ( Macaca mulatta ) experimentally infected with simian immunodeficiency virus (SIV) varies significantly from animal to animal. Some animals die within 2 months while others survive for more than 5 years, even when identical inocula are used. This diversity in survival creates a significant problem in the design of therapeutic and vaccine trials using the SIV–macaque model because the use of small numbers of animals may provide results that are misleading. Identifying an in vitro assay that could determine the survival of monkeys prior to infection would prove extremely useful for stratifying experimental groups. Analysis of the survival of a cohort of 59 control animals obtained from over a decade of vaccine and therapeutic trials has demonstrated that the ability of peripheral blood mononuclear cells (PBMC) from a naïve animal to produce virus in vitro was highly predictive of disease progression in vivo following experimental inoculation. Animals classified in vitro as high producers of virus progressed to disease significantly more rapidly than animals classified as either low ( P =0·002) or intermediate ( P =0·013) producers of virus. The hierarchy of high and low virus production was maintained in purified CD4 + T cell cultures, indicating that this phenotype is an intrinsic property of the CD4 + T cell itself. These findings should significantly aid in the design of vaccine and therapeutic trials using the SIV–macaque model. Furthermore, since these studies suggest that the rate of virus replication is controlled by innate characteristics of the individual, they provide new insight into the pathogenesis of AIDS.</description><identifier>ISSN: 0022-1317</identifier><identifier>EISSN: 1465-2099</identifier><identifier>DOI: 10.1099/0022-1317-81-10-2441</identifier><identifier>PMID: 10993932</identifier><language>eng</language><publisher>England: Soc General Microbiol</publisher><subject>Acquired Immunodeficiency Syndrome - physiopathology ; Acquired Immunodeficiency Syndrome - virology ; Animals ; CD4 antigen ; CD4-Positive T-Lymphocytes - virology ; Cohort Studies ; Disease Progression ; Female ; In Vitro Techniques ; Leukocytes, Mononuclear - virology ; Macaca mulatta ; Male ; Phenotype ; Simian immunodeficiency virus ; Simian Immunodeficiency Virus - physiology ; Survival Rate ; Virus Replication</subject><ispartof>Journal of general virology, 2000-10, Vol.81 (10), p.2441-2449</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c480t-4188e51375128b05df49f9f5685d8258accd71dedefd05fec1f018dc75c53ac03</citedby><cites>FETCH-LOGICAL-c480t-4188e51375128b05df49f9f5685d8258accd71dedefd05fec1f018dc75c53ac03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3746,3747,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10993932$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Seman, Amy L</creatorcontrib><creatorcontrib>Pewen, William F</creatorcontrib><creatorcontrib>Fresh, Lynn F</creatorcontrib><creatorcontrib>Martin, Louis N</creatorcontrib><creatorcontrib>Murphey-Corb, Michael</creatorcontrib><title>The replicative capacity of rhesus macaque peripheral blood mononuclear cells for simian immunodeficiency virus in vitro is predictive of the rate of progression to AIDS in vivo</title><title>Journal of general virology</title><addtitle>J Gen Virol</addtitle><description>Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Room E1240 Biomedical Science Tower, Pittsburgh, PA 15261, USA 1 Tulane Regional Primate Research Center, 18703 Three Rivers Road, Covington, LA 70433, USA 2 Author for correspondence: Michael Murphey-Corb. Fax +1 412 648 1448. e-mail mcorb{at}pitt.edu Survival of rhesus macaques ( Macaca mulatta ) experimentally infected with simian immunodeficiency virus (SIV) varies significantly from animal to animal. Some animals die within 2 months while others survive for more than 5 years, even when identical inocula are used. This diversity in survival creates a significant problem in the design of therapeutic and vaccine trials using the SIV–macaque model because the use of small numbers of animals may provide results that are misleading. Identifying an in vitro assay that could determine the survival of monkeys prior to infection would prove extremely useful for stratifying experimental groups. Analysis of the survival of a cohort of 59 control animals obtained from over a decade of vaccine and therapeutic trials has demonstrated that the ability of peripheral blood mononuclear cells (PBMC) from a naïve animal to produce virus in vitro was highly predictive of disease progression in vivo following experimental inoculation. Animals classified in vitro as high producers of virus progressed to disease significantly more rapidly than animals classified as either low ( P =0·002) or intermediate ( P =0·013) producers of virus. The hierarchy of high and low virus production was maintained in purified CD4 + T cell cultures, indicating that this phenotype is an intrinsic property of the CD4 + T cell itself. These findings should significantly aid in the design of vaccine and therapeutic trials using the SIV–macaque model. Furthermore, since these studies suggest that the rate of virus replication is controlled by innate characteristics of the individual, they provide new insight into the pathogenesis of AIDS.</description><subject>Acquired Immunodeficiency Syndrome - physiopathology</subject><subject>Acquired Immunodeficiency Syndrome - virology</subject><subject>Animals</subject><subject>CD4 antigen</subject><subject>CD4-Positive T-Lymphocytes - virology</subject><subject>Cohort Studies</subject><subject>Disease Progression</subject><subject>Female</subject><subject>In Vitro Techniques</subject><subject>Leukocytes, Mononuclear - virology</subject><subject>Macaca mulatta</subject><subject>Male</subject><subject>Phenotype</subject><subject>Simian immunodeficiency virus</subject><subject>Simian Immunodeficiency Virus - physiology</subject><subject>Survival Rate</subject><subject>Virus Replication</subject><issn>0022-1317</issn><issn>1465-2099</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u1TAQhS1ERS-FN0DIKyQWobYTJ86yKn-VKrFou7Z87fHNoCQOdnLRfSzeEKepUHesPLK-OXNmDiHvOPvEWdteMiZEwUveFIoXnBWiqvgLsuNVLQuRgZdk9w85J69T-skYryrZvCLnq0DZlmJH_tx3QCNMPVoz4xGoNZOxOJ9o8DR2kJZEB2PNrwXoBBGnDqLp6b4PwdEhjGFcbA8mUgt9n6gPkSYc0IwUh2EZgwOPFmG0J3rEmMVwzMUcA8VEpwgO7ePYPG1enZj5sZ5iOERICcNI50Cvbj7fbZ3H8IacedMnePv0XpCHr1_ur78Xtz--3Vxf3Ra2UmwuKq4USF42kgu1Z9L5qvWtl7WSTgmpjLWu4Q6yQcekB8s948rZRlpZGsvKC_Jh081e8vZp1gOmdUszQliSboSom7qt_wvyRjaCCZXBagNtDClF8HqKOJh40pzpNRK9BqbXwLTi6-eaaW57_6S_7Adwz5q2EDPwcQM6PHS_MYI-wDhgnrLHoPPZn4n9Bd9rr0I</recordid><startdate>20001001</startdate><enddate>20001001</enddate><creator>Seman, Amy L</creator><creator>Pewen, William F</creator><creator>Fresh, Lynn F</creator><creator>Martin, Louis N</creator><creator>Murphey-Corb, Michael</creator><general>Soc General Microbiol</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20001001</creationdate><title>The replicative capacity of rhesus macaque peripheral blood mononuclear cells for simian immunodeficiency virus in vitro is predictive of the rate of progression to AIDS in vivo</title><author>Seman, Amy L ; Pewen, William F ; Fresh, Lynn F ; Martin, Louis N ; Murphey-Corb, Michael</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c480t-4188e51375128b05df49f9f5685d8258accd71dedefd05fec1f018dc75c53ac03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Acquired Immunodeficiency Syndrome - physiopathology</topic><topic>Acquired Immunodeficiency Syndrome - virology</topic><topic>Animals</topic><topic>CD4 antigen</topic><topic>CD4-Positive T-Lymphocytes - virology</topic><topic>Cohort Studies</topic><topic>Disease Progression</topic><topic>Female</topic><topic>In Vitro Techniques</topic><topic>Leukocytes, Mononuclear - virology</topic><topic>Macaca mulatta</topic><topic>Male</topic><topic>Phenotype</topic><topic>Simian immunodeficiency virus</topic><topic>Simian Immunodeficiency Virus - physiology</topic><topic>Survival Rate</topic><topic>Virus Replication</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Seman, Amy L</creatorcontrib><creatorcontrib>Pewen, William F</creatorcontrib><creatorcontrib>Fresh, Lynn F</creatorcontrib><creatorcontrib>Martin, Louis N</creatorcontrib><creatorcontrib>Murphey-Corb, Michael</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of general virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Seman, Amy L</au><au>Pewen, William F</au><au>Fresh, Lynn F</au><au>Martin, Louis N</au><au>Murphey-Corb, Michael</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The replicative capacity of rhesus macaque peripheral blood mononuclear cells for simian immunodeficiency virus in vitro is predictive of the rate of progression to AIDS in vivo</atitle><jtitle>Journal of general virology</jtitle><addtitle>J Gen Virol</addtitle><date>2000-10-01</date><risdate>2000</risdate><volume>81</volume><issue>10</issue><spage>2441</spage><epage>2449</epage><pages>2441-2449</pages><issn>0022-1317</issn><eissn>1465-2099</eissn><abstract>Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Room E1240 Biomedical Science Tower, Pittsburgh, PA 15261, USA 1 Tulane Regional Primate Research Center, 18703 Three Rivers Road, Covington, LA 70433, USA 2 Author for correspondence: Michael Murphey-Corb. Fax +1 412 648 1448. e-mail mcorb{at}pitt.edu Survival of rhesus macaques ( Macaca mulatta ) experimentally infected with simian immunodeficiency virus (SIV) varies significantly from animal to animal. Some animals die within 2 months while others survive for more than 5 years, even when identical inocula are used. This diversity in survival creates a significant problem in the design of therapeutic and vaccine trials using the SIV–macaque model because the use of small numbers of animals may provide results that are misleading. Identifying an in vitro assay that could determine the survival of monkeys prior to infection would prove extremely useful for stratifying experimental groups. Analysis of the survival of a cohort of 59 control animals obtained from over a decade of vaccine and therapeutic trials has demonstrated that the ability of peripheral blood mononuclear cells (PBMC) from a naïve animal to produce virus in vitro was highly predictive of disease progression in vivo following experimental inoculation. Animals classified in vitro as high producers of virus progressed to disease significantly more rapidly than animals classified as either low ( P =0·002) or intermediate ( P =0·013) producers of virus. The hierarchy of high and low virus production was maintained in purified CD4 + T cell cultures, indicating that this phenotype is an intrinsic property of the CD4 + T cell itself. These findings should significantly aid in the design of vaccine and therapeutic trials using the SIV–macaque model. Furthermore, since these studies suggest that the rate of virus replication is controlled by innate characteristics of the individual, they provide new insight into the pathogenesis of AIDS.</abstract><cop>England</cop><pub>Soc General Microbiol</pub><pmid>10993932</pmid><doi>10.1099/0022-1317-81-10-2441</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0022-1317
ispartof Journal of general virology, 2000-10, Vol.81 (10), p.2441-2449
issn 0022-1317
1465-2099
language eng
recordid cdi_proquest_miscellaneous_72267696
source MEDLINE; Microbiology Society; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection
subjects Acquired Immunodeficiency Syndrome - physiopathology
Acquired Immunodeficiency Syndrome - virology
Animals
CD4 antigen
CD4-Positive T-Lymphocytes - virology
Cohort Studies
Disease Progression
Female
In Vitro Techniques
Leukocytes, Mononuclear - virology
Macaca mulatta
Male
Phenotype
Simian immunodeficiency virus
Simian Immunodeficiency Virus - physiology
Survival Rate
Virus Replication
title The replicative capacity of rhesus macaque peripheral blood mononuclear cells for simian immunodeficiency virus in vitro is predictive of the rate of progression to AIDS in vivo
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T02%3A39%3A03IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20replicative%20capacity%20of%20rhesus%20macaque%20peripheral%20blood%20mononuclear%20cells%20for%20simian%20immunodeficiency%20virus%20in%20vitro%20is%20predictive%20of%20the%20rate%20of%20progression%20to%20AIDS%20in%20vivo&rft.jtitle=Journal%20of%20general%20virology&rft.au=Seman,%20Amy%20L&rft.date=2000-10-01&rft.volume=81&rft.issue=10&rft.spage=2441&rft.epage=2449&rft.pages=2441-2449&rft.issn=0022-1317&rft.eissn=1465-2099&rft_id=info:doi/10.1099/0022-1317-81-10-2441&rft_dat=%3Cproquest_cross%3E17572028%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=17572028&rft_id=info:pmid/10993932&rfr_iscdi=true