Quantitation of Na+-K+-2Cl− Cotransport Splice Variants in Human Tissues Using Kinetic Polymerase Chain Reaction

A kinetic reverse transcription–polymerase chain reaction (RT-PCR)-based assay is described that can discriminate and quantitate differentially spliced mRNAs. This assay should be generally applicable for high-throughput quantitation of differentially spliced transcripts. The utility of this method...

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Veröffentlicht in:	Analytical biochemistry 2001-11, Vol.298 (2), p.218-230
Hauptverfasser:	Vibat, Cecile Rose T., Holland, Michael J., Kang, John J., Putney, Luanna K., O'Donnell, Martha E.
Format:	Artikel
Sprache:	eng
Schlagworte:	alternative splicing Alternative Splicing - genetics Amino Acid Sequence Base Sequence brain Cells, Cultured Chlorides - metabolism cotransport DNA Primers - chemistry Glaucoma - metabolism Humans kinetic RT-PCR Kinetics Molecular Sequence Data Potassium - metabolism Reverse Transcriptase Polymerase Chain Reaction RNA RNA, Messenger - metabolism Sensitivity and Specificity Sequence Homology, Nucleic Acid Sodium - metabolism Sodium-Potassium-Chloride Symporters - genetics Tissue Distribution trabecular meshwork Trabecular Meshwork - metabolism
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