Mapping peroxidase in plant tissues by scanning electrochemical microscopy
Scanning electrochemical microscopy has been firstly used to map the enzymatic activity in natural plant tissues. The peroxidase (POD) was maintained in its original state in the celery ( Apium graveolens L.) tissues and electrochemically visualized under its native environment. Ferrocenemethanol (F...
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| Veröffentlicht in: | Bioelectrochemistry (Amsterdam, Netherlands) Netherlands), 2001-11, Vol.54 (2), p.151-156 |
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| container_title | Bioelectrochemistry (Amsterdam, Netherlands) |
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| creator | Zhou, Huafang Shiku, Hitoshi Kasai, Shigenobu Noda, Hiroyuki Matsue, Tomokazu Ohya-Nishiguchi, Hiroaki Kamada, Hitoshi |
| description | Scanning electrochemical microscopy has been firstly used to map the enzymatic activity in natural plant tissues. The peroxidase (POD) was maintained in its original state in the celery (
Apium graveolens L.) tissues and electrochemically visualized under its native environment. Ferrocenemethanol (FMA) was selected as a mediator to probe the POD in celery tissues based on the fact that POD catalyzed the oxidation of FMA by H
2O
2 to increase FMA
+ concentration. Two-dimensional reduction current profiles for FMA
+ produced images indicating the distribution and activity of the POD at the surface of the celery tissues. These images showed that the POD was widely distributed in the celery tissues, and larger amounts were found in some special regions such as the center of celery stem and around some vascular bundles. |
| doi_str_mv | 10.1016/S1567-5394(01)00123-2 |
| format | Article |
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Apium graveolens L.) tissues and electrochemically visualized under its native environment. Ferrocenemethanol (FMA) was selected as a mediator to probe the POD in celery tissues based on the fact that POD catalyzed the oxidation of FMA by H
2O
2 to increase FMA
+ concentration. Two-dimensional reduction current profiles for FMA
+ produced images indicating the distribution and activity of the POD at the surface of the celery tissues. These images showed that the POD was widely distributed in the celery tissues, and larger amounts were found in some special regions such as the center of celery stem and around some vascular bundles.</description><identifier>ISSN: 1567-5394</identifier><identifier>EISSN: 1878-562X</identifier><identifier>DOI: 10.1016/S1567-5394(01)00123-2</identifier><identifier>PMID: 11694396</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Apium graveolens - cytology ; Apium graveolens - enzymology ; Apium graveolens - ultrastructure ; Celery tissues ; Electrochemistry ; Ferrous Compounds - metabolism ; Microelectrodes ; Microscopy, Electron, Scanning - instrumentation ; Microscopy, Electron, Scanning - methods ; Molecular Probes - metabolism ; Peroxidase ; Peroxidase - metabolism ; Plant Cells ; Plants - enzymology ; Plants - ultrastructure ; Scanning electrochemical microscopy ; Tissue Distribution</subject><ispartof>Bioelectrochemistry (Amsterdam, Netherlands), 2001-11, Vol.54 (2), p.151-156</ispartof><rights>2001 Elsevier Science B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c427t-ac4fca807f26549d8495faab8aa72e8a70674d31939c2a7ea427394bff8d6d233</citedby><cites>FETCH-LOGICAL-c427t-ac4fca807f26549d8495faab8aa72e8a70674d31939c2a7ea427394bff8d6d233</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1567539401001232$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11694396$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhou, Huafang</creatorcontrib><creatorcontrib>Shiku, Hitoshi</creatorcontrib><creatorcontrib>Kasai, Shigenobu</creatorcontrib><creatorcontrib>Noda, Hiroyuki</creatorcontrib><creatorcontrib>Matsue, Tomokazu</creatorcontrib><creatorcontrib>Ohya-Nishiguchi, Hiroaki</creatorcontrib><creatorcontrib>Kamada, Hitoshi</creatorcontrib><title>Mapping peroxidase in plant tissues by scanning electrochemical microscopy</title><title>Bioelectrochemistry (Amsterdam, Netherlands)</title><addtitle>Bioelectrochemistry</addtitle><description>Scanning electrochemical microscopy has been firstly used to map the enzymatic activity in natural plant tissues. The peroxidase (POD) was maintained in its original state in the celery (
Apium graveolens L.) tissues and electrochemically visualized under its native environment. Ferrocenemethanol (FMA) was selected as a mediator to probe the POD in celery tissues based on the fact that POD catalyzed the oxidation of FMA by H
2O
2 to increase FMA
+ concentration. Two-dimensional reduction current profiles for FMA
+ produced images indicating the distribution and activity of the POD at the surface of the celery tissues. These images showed that the POD was widely distributed in the celery tissues, and larger amounts were found in some special regions such as the center of celery stem and around some vascular bundles.</description><subject>Apium graveolens - cytology</subject><subject>Apium graveolens - enzymology</subject><subject>Apium graveolens - ultrastructure</subject><subject>Celery tissues</subject><subject>Electrochemistry</subject><subject>Ferrous Compounds - metabolism</subject><subject>Microelectrodes</subject><subject>Microscopy, Electron, Scanning - instrumentation</subject><subject>Microscopy, Electron, Scanning - methods</subject><subject>Molecular Probes - metabolism</subject><subject>Peroxidase</subject><subject>Peroxidase - metabolism</subject><subject>Plant Cells</subject><subject>Plants - enzymology</subject><subject>Plants - ultrastructure</subject><subject>Scanning electrochemical microscopy</subject><subject>Tissue Distribution</subject><issn>1567-5394</issn><issn>1878-562X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkD1PwzAURS0EoqXwE0CZEAwB23H8MSFU8akiBkBisxznBYzSJNgpov8epy1iZLE9nOt330HokOAzggk_fyI5F2meKXaCySnGhGYp3UJjIoVMc05ft-P7FxmhvRA-MMaSiHwXjQjhimWKj9H9g-k617wlHfj225UmQOKapKtN0ye9C2EBISmWSbCmaQYOarC9b-07zJ01dRJP3wbbdst9tFOZOsDB5p6gl-ur5-ltOnu8uZtezlLLqOhTY1lljcSiojxnqpRM5ZUxhTRGUJBGYC5YmRGVKUuNABNTcYWiqmTJS5plE3S8_rfz7Wes1-u5CxbqWBnaRdCC0pxxoSKYr8GhYfBQ6c67ufFLTbAeJOqVRD0Y0pjolURNY-5oM2BRzKH8S22sReBiDUBc88uB18E6aCyUzkc7umzdPyN-AJyRgnE</recordid><startdate>20011101</startdate><enddate>20011101</enddate><creator>Zhou, Huafang</creator><creator>Shiku, Hitoshi</creator><creator>Kasai, Shigenobu</creator><creator>Noda, Hiroyuki</creator><creator>Matsue, Tomokazu</creator><creator>Ohya-Nishiguchi, Hiroaki</creator><creator>Kamada, Hitoshi</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20011101</creationdate><title>Mapping peroxidase in plant tissues by scanning electrochemical microscopy</title><author>Zhou, Huafang ; Shiku, Hitoshi ; Kasai, Shigenobu ; Noda, Hiroyuki ; Matsue, Tomokazu ; Ohya-Nishiguchi, Hiroaki ; Kamada, Hitoshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c427t-ac4fca807f26549d8495faab8aa72e8a70674d31939c2a7ea427394bff8d6d233</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Apium graveolens - cytology</topic><topic>Apium graveolens - enzymology</topic><topic>Apium graveolens - ultrastructure</topic><topic>Celery tissues</topic><topic>Electrochemistry</topic><topic>Ferrous Compounds - metabolism</topic><topic>Microelectrodes</topic><topic>Microscopy, Electron, Scanning - instrumentation</topic><topic>Microscopy, Electron, Scanning - methods</topic><topic>Molecular Probes - metabolism</topic><topic>Peroxidase</topic><topic>Peroxidase - metabolism</topic><topic>Plant Cells</topic><topic>Plants - enzymology</topic><topic>Plants - ultrastructure</topic><topic>Scanning electrochemical microscopy</topic><topic>Tissue Distribution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhou, Huafang</creatorcontrib><creatorcontrib>Shiku, Hitoshi</creatorcontrib><creatorcontrib>Kasai, Shigenobu</creatorcontrib><creatorcontrib>Noda, Hiroyuki</creatorcontrib><creatorcontrib>Matsue, Tomokazu</creatorcontrib><creatorcontrib>Ohya-Nishiguchi, Hiroaki</creatorcontrib><creatorcontrib>Kamada, Hitoshi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Bioelectrochemistry (Amsterdam, Netherlands)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhou, Huafang</au><au>Shiku, Hitoshi</au><au>Kasai, Shigenobu</au><au>Noda, Hiroyuki</au><au>Matsue, Tomokazu</au><au>Ohya-Nishiguchi, Hiroaki</au><au>Kamada, Hitoshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mapping peroxidase in plant tissues by scanning electrochemical microscopy</atitle><jtitle>Bioelectrochemistry (Amsterdam, Netherlands)</jtitle><addtitle>Bioelectrochemistry</addtitle><date>2001-11-01</date><risdate>2001</risdate><volume>54</volume><issue>2</issue><spage>151</spage><epage>156</epage><pages>151-156</pages><issn>1567-5394</issn><eissn>1878-562X</eissn><abstract>Scanning electrochemical microscopy has been firstly used to map the enzymatic activity in natural plant tissues. The peroxidase (POD) was maintained in its original state in the celery (
Apium graveolens L.) tissues and electrochemically visualized under its native environment. Ferrocenemethanol (FMA) was selected as a mediator to probe the POD in celery tissues based on the fact that POD catalyzed the oxidation of FMA by H
2O
2 to increase FMA
+ concentration. Two-dimensional reduction current profiles for FMA
+ produced images indicating the distribution and activity of the POD at the surface of the celery tissues. These images showed that the POD was widely distributed in the celery tissues, and larger amounts were found in some special regions such as the center of celery stem and around some vascular bundles.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>11694396</pmid><doi>10.1016/S1567-5394(01)00123-2</doi><tpages>6</tpages></addata></record> |
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| subjects | Apium graveolens - cytology Apium graveolens - enzymology Apium graveolens - ultrastructure Celery tissues Electrochemistry Ferrous Compounds - metabolism Microelectrodes Microscopy, Electron, Scanning - instrumentation Microscopy, Electron, Scanning - methods Molecular Probes - metabolism Peroxidase Peroxidase - metabolism Plant Cells Plants - enzymology Plants - ultrastructure Scanning electrochemical microscopy Tissue Distribution |
| title | Mapping peroxidase in plant tissues by scanning electrochemical microscopy |
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