Regulation of insulin-like growth factor-I and of insulin-like growth factor binding protein-1, -3 and -4 in cocultures of rat hepatocytes and Kupffer cells by interleukin-6
Background/Aims : Catabolism is associated with decreased serum concentrations of insulin-like growth factor (IGF)-I and insulin-like growth factor binding protein (IGFBP)-3 associated with elevated IGFBP-3 protease activity and increased concentrations of IGFBP-1 and -4. The effects of the acute ph...
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| Veröffentlicht in: | Journal of hepatology 2001-11, Vol.35 (5), p.558-567 |
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| container_title | Journal of hepatology |
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| creator | Lelbach, Adam Scharf, Jens-Gerd Ramadori, Giuliano |
| description | Background/Aims
: Catabolism is associated with decreased serum concentrations of insulin-like growth factor (IGF)-I and insulin-like growth factor binding protein (IGFBP)-3 associated with elevated IGFBP-3 protease activity and increased concentrations of IGFBP-1 and -4. The effects of the acute phase mediators interleukin (IL)-6, IL-1
β and tumor necrosis factor
α (TNF
α) on the biosynthesis of IGF-I and IGFBPs were studied in primary rat liver cells.
Methods
: mRNA levels of IGF-I and of IGFBPs were analyzed by Northern blotting, secretion of IGFBPs by [
125
I]IGF-I ligand blotting. Proteolytic activity was measured using iodinated recombinant IGFBP-3 as the substrate.
Results
: In hepatocytes, Kupffer cells (KC) and cocultures of hepatocytes with KC, IL-6 reduced IGF-I biosynthesis dose-dependently. IL-6 stimulated mRNA expression and protein secretion of IGFBP-1 and -4 in hepatocytes and that of IGFBP-3 in KC, respectively. In cocultures, biosynthesis of IGFBP-1, -3 and -4 was increased dose-dependently by IL-6, while the effects of IL-1
β or TNF
α were less prominent. At neutral pH, proteolytic activity against IGFBP-3 was not detected in media of cocultures treated with IL-6.
Conclusions
: The alterations of IGF-I, IGFBP-1 and -4 observed in catabolism correlate with the effects of IL-6 on the biosynthesis of these components in primary rat liver cells, while a neutral IGFBP-3 protease was not detectable. |
| doi_str_mv | 10.1016/S0168-8278(01)00170-2 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_72254510</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0168827801001702</els_id><sourcerecordid>72254510</sourcerecordid><originalsourceid>FETCH-LOGICAL-c457t-a4c369099f605317a765e89d0e5630e4646c40c4cec11290ec1a9e8a0cbc08453</originalsourceid><addsrcrecordid>eNqFkd1q3DAQhUVpaDZpH6FFNy0pRO3IlmX7qoTQn9BAoT_XQjseb9R4rY0kN-xD9R0r7y7NXYtAA-I7Z2Z0GHsu4Y0Eqd9-y1cjmqJuzkC-BpA1iOIRW0gNIEAr-Zgt_iLH7CTGnwBQQquesGMpdQs1wIL9_kqrabDJ-ZH7nrsxToMbxeBuia-Cv083vLeYfBBX3I7dvxm-dGPnxhXfBJ8oI_Kci3KnEyrrOHqchjQFirNRsInf0MYmj9uUn2bu87TpewocaRgiX26zKlEYaLrNdvopO-rtEOnZoZ6yHx_ef7_8JK6_fLy6vLgWqKo6CauwzAu2ba-hKmVta11R03ZAlS6BlFYaFaBCQimLFnKxLTUWcInQqKo8Za_2vnmRu4liMmsX55HsSH6Kpi6KSlUSMljtQQw-xkC92QS3tmFrJJg5J7PLycwhGJBml5Mpsu7FocG0XFP3oDoEk4GXB8BGtEMf7IguPnAqH6hV5t7tOcrf8ctRMBEdjUidC4TJdN79Z5Q_LiKwLw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>72254510</pqid></control><display><type>article</type><title>Regulation of insulin-like growth factor-I and of insulin-like growth factor binding protein-1, -3 and -4 in cocultures of rat hepatocytes and Kupffer cells by interleukin-6</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><creator>Lelbach, Adam ; Scharf, Jens-Gerd ; Ramadori, Giuliano</creator><creatorcontrib>Lelbach, Adam ; Scharf, Jens-Gerd ; Ramadori, Giuliano</creatorcontrib><description>Background/Aims
: Catabolism is associated with decreased serum concentrations of insulin-like growth factor (IGF)-I and insulin-like growth factor binding protein (IGFBP)-3 associated with elevated IGFBP-3 protease activity and increased concentrations of IGFBP-1 and -4. The effects of the acute phase mediators interleukin (IL)-6, IL-1
β and tumor necrosis factor
α (TNF
α) on the biosynthesis of IGF-I and IGFBPs were studied in primary rat liver cells.
Methods
: mRNA levels of IGF-I and of IGFBPs were analyzed by Northern blotting, secretion of IGFBPs by [
125
I]IGF-I ligand blotting. Proteolytic activity was measured using iodinated recombinant IGFBP-3 as the substrate.
Results
: In hepatocytes, Kupffer cells (KC) and cocultures of hepatocytes with KC, IL-6 reduced IGF-I biosynthesis dose-dependently. IL-6 stimulated mRNA expression and protein secretion of IGFBP-1 and -4 in hepatocytes and that of IGFBP-3 in KC, respectively. In cocultures, biosynthesis of IGFBP-1, -3 and -4 was increased dose-dependently by IL-6, while the effects of IL-1
β or TNF
α were less prominent. At neutral pH, proteolytic activity against IGFBP-3 was not detected in media of cocultures treated with IL-6.
Conclusions
: The alterations of IGF-I, IGFBP-1 and -4 observed in catabolism correlate with the effects of IL-6 on the biosynthesis of these components in primary rat liver cells, while a neutral IGFBP-3 protease was not detectable.</description><identifier>ISSN: 0168-8278</identifier><identifier>EISSN: 1600-0641</identifier><identifier>DOI: 10.1016/S0168-8278(01)00170-2</identifier><identifier>PMID: 11690700</identifier><identifier>CODEN: JOHEEC</identifier><language>eng</language><publisher>Oxford: Elsevier B.V</publisher><subject>Animals ; Biological and medical sciences ; Coculture ; Coculture Techniques ; DNA Probes ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation - drug effects ; Gene Expression Regulation - physiology ; Hepatocytes ; Hepatocytes - cytology ; Hepatocytes - drug effects ; Hepatocytes - metabolism ; Insulin-Like Growth Factor Binding Protein 1 - genetics ; Insulin-Like Growth Factor Binding Protein 3 - genetics ; Insulin-Like Growth Factor Binding Protein 4 - genetics ; Insulin-like growth factor binding protein-3 protease ; Insulin-like growth factor binding proteins ; Insulin-Like Growth Factor I - genetics ; Insulin-like growth factor-I ; Interleukin-6 ; Interleukin-6 - pharmacology ; Kinetics ; Kupffer cells ; Kupffer Cells - cytology ; Kupffer Cells - drug effects ; Kupffer Cells - metabolism ; Liver. Bile. Biliary tracts ; Male ; Rats ; Rats, Wistar ; RNA, Messenger - genetics ; Transcription, Genetic - drug effects ; Vertebrates: digestive system</subject><ispartof>Journal of hepatology, 2001-11, Vol.35 (5), p.558-567</ispartof><rights>2001 European Association for the Study of the Liver</rights><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c457t-a4c369099f605317a765e89d0e5630e4646c40c4cec11290ec1a9e8a0cbc08453</citedby><cites>FETCH-LOGICAL-c457t-a4c369099f605317a765e89d0e5630e4646c40c4cec11290ec1a9e8a0cbc08453</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0168827801001702$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14141074$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11690700$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lelbach, Adam</creatorcontrib><creatorcontrib>Scharf, Jens-Gerd</creatorcontrib><creatorcontrib>Ramadori, Giuliano</creatorcontrib><title>Regulation of insulin-like growth factor-I and of insulin-like growth factor binding protein-1, -3 and -4 in cocultures of rat hepatocytes and Kupffer cells by interleukin-6</title><title>Journal of hepatology</title><addtitle>J Hepatol</addtitle><description>Background/Aims
: Catabolism is associated with decreased serum concentrations of insulin-like growth factor (IGF)-I and insulin-like growth factor binding protein (IGFBP)-3 associated with elevated IGFBP-3 protease activity and increased concentrations of IGFBP-1 and -4. The effects of the acute phase mediators interleukin (IL)-6, IL-1
β and tumor necrosis factor
α (TNF
α) on the biosynthesis of IGF-I and IGFBPs were studied in primary rat liver cells.
Methods
: mRNA levels of IGF-I and of IGFBPs were analyzed by Northern blotting, secretion of IGFBPs by [
125
I]IGF-I ligand blotting. Proteolytic activity was measured using iodinated recombinant IGFBP-3 as the substrate.
Results
: In hepatocytes, Kupffer cells (KC) and cocultures of hepatocytes with KC, IL-6 reduced IGF-I biosynthesis dose-dependently. IL-6 stimulated mRNA expression and protein secretion of IGFBP-1 and -4 in hepatocytes and that of IGFBP-3 in KC, respectively. In cocultures, biosynthesis of IGFBP-1, -3 and -4 was increased dose-dependently by IL-6, while the effects of IL-1
β or TNF
α were less prominent. At neutral pH, proteolytic activity against IGFBP-3 was not detected in media of cocultures treated with IL-6.
Conclusions
: The alterations of IGF-I, IGFBP-1 and -4 observed in catabolism correlate with the effects of IL-6 on the biosynthesis of these components in primary rat liver cells, while a neutral IGFBP-3 protease was not detectable.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Coculture</subject><subject>Coculture Techniques</subject><subject>DNA Probes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Gene Expression Regulation - physiology</subject><subject>Hepatocytes</subject><subject>Hepatocytes - cytology</subject><subject>Hepatocytes - drug effects</subject><subject>Hepatocytes - metabolism</subject><subject>Insulin-Like Growth Factor Binding Protein 1 - genetics</subject><subject>Insulin-Like Growth Factor Binding Protein 3 - genetics</subject><subject>Insulin-Like Growth Factor Binding Protein 4 - genetics</subject><subject>Insulin-like growth factor binding protein-3 protease</subject><subject>Insulin-like growth factor binding proteins</subject><subject>Insulin-Like Growth Factor I - genetics</subject><subject>Insulin-like growth factor-I</subject><subject>Interleukin-6</subject><subject>Interleukin-6 - pharmacology</subject><subject>Kinetics</subject><subject>Kupffer cells</subject><subject>Kupffer Cells - cytology</subject><subject>Kupffer Cells - drug effects</subject><subject>Kupffer Cells - metabolism</subject><subject>Liver. Bile. Biliary tracts</subject><subject>Male</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>RNA, Messenger - genetics</subject><subject>Transcription, Genetic - drug effects</subject><subject>Vertebrates: digestive system</subject><issn>0168-8278</issn><issn>1600-0641</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkd1q3DAQhUVpaDZpH6FFNy0pRO3IlmX7qoTQn9BAoT_XQjseb9R4rY0kN-xD9R0r7y7NXYtAA-I7Z2Z0GHsu4Y0Eqd9-y1cjmqJuzkC-BpA1iOIRW0gNIEAr-Zgt_iLH7CTGnwBQQquesGMpdQs1wIL9_kqrabDJ-ZH7nrsxToMbxeBuia-Cv083vLeYfBBX3I7dvxm-dGPnxhXfBJ8oI_Kci3KnEyrrOHqchjQFirNRsInf0MYmj9uUn2bu87TpewocaRgiX26zKlEYaLrNdvopO-rtEOnZoZ6yHx_ef7_8JK6_fLy6vLgWqKo6CauwzAu2ba-hKmVta11R03ZAlS6BlFYaFaBCQimLFnKxLTUWcInQqKo8Za_2vnmRu4liMmsX55HsSH6Kpi6KSlUSMljtQQw-xkC92QS3tmFrJJg5J7PLycwhGJBml5Mpsu7FocG0XFP3oDoEk4GXB8BGtEMf7IguPnAqH6hV5t7tOcrf8ctRMBEdjUidC4TJdN79Z5Q_LiKwLw</recordid><startdate>20011101</startdate><enddate>20011101</enddate><creator>Lelbach, Adam</creator><creator>Scharf, Jens-Gerd</creator><creator>Ramadori, Giuliano</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20011101</creationdate><title>Regulation of insulin-like growth factor-I and of insulin-like growth factor binding protein-1, -3 and -4 in cocultures of rat hepatocytes and Kupffer cells by interleukin-6</title><author>Lelbach, Adam ; Scharf, Jens-Gerd ; Ramadori, Giuliano</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c457t-a4c369099f605317a765e89d0e5630e4646c40c4cec11290ec1a9e8a0cbc08453</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Coculture</topic><topic>Coculture Techniques</topic><topic>DNA Probes</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Gene Expression Regulation - physiology</topic><topic>Hepatocytes</topic><topic>Hepatocytes - cytology</topic><topic>Hepatocytes - drug effects</topic><topic>Hepatocytes - metabolism</topic><topic>Insulin-Like Growth Factor Binding Protein 1 - genetics</topic><topic>Insulin-Like Growth Factor Binding Protein 3 - genetics</topic><topic>Insulin-Like Growth Factor Binding Protein 4 - genetics</topic><topic>Insulin-like growth factor binding protein-3 protease</topic><topic>Insulin-like growth factor binding proteins</topic><topic>Insulin-Like Growth Factor I - genetics</topic><topic>Insulin-like growth factor-I</topic><topic>Interleukin-6</topic><topic>Interleukin-6 - pharmacology</topic><topic>Kinetics</topic><topic>Kupffer cells</topic><topic>Kupffer Cells - cytology</topic><topic>Kupffer Cells - drug effects</topic><topic>Kupffer Cells - metabolism</topic><topic>Liver. Bile. Biliary tracts</topic><topic>Male</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>RNA, Messenger - genetics</topic><topic>Transcription, Genetic - drug effects</topic><topic>Vertebrates: digestive system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lelbach, Adam</creatorcontrib><creatorcontrib>Scharf, Jens-Gerd</creatorcontrib><creatorcontrib>Ramadori, Giuliano</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of hepatology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lelbach, Adam</au><au>Scharf, Jens-Gerd</au><au>Ramadori, Giuliano</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Regulation of insulin-like growth factor-I and of insulin-like growth factor binding protein-1, -3 and -4 in cocultures of rat hepatocytes and Kupffer cells by interleukin-6</atitle><jtitle>Journal of hepatology</jtitle><addtitle>J Hepatol</addtitle><date>2001-11-01</date><risdate>2001</risdate><volume>35</volume><issue>5</issue><spage>558</spage><epage>567</epage><pages>558-567</pages><issn>0168-8278</issn><eissn>1600-0641</eissn><coden>JOHEEC</coden><abstract>Background/Aims
: Catabolism is associated with decreased serum concentrations of insulin-like growth factor (IGF)-I and insulin-like growth factor binding protein (IGFBP)-3 associated with elevated IGFBP-3 protease activity and increased concentrations of IGFBP-1 and -4. The effects of the acute phase mediators interleukin (IL)-6, IL-1
β and tumor necrosis factor
α (TNF
α) on the biosynthesis of IGF-I and IGFBPs were studied in primary rat liver cells.
Methods
: mRNA levels of IGF-I and of IGFBPs were analyzed by Northern blotting, secretion of IGFBPs by [
125
I]IGF-I ligand blotting. Proteolytic activity was measured using iodinated recombinant IGFBP-3 as the substrate.
Results
: In hepatocytes, Kupffer cells (KC) and cocultures of hepatocytes with KC, IL-6 reduced IGF-I biosynthesis dose-dependently. IL-6 stimulated mRNA expression and protein secretion of IGFBP-1 and -4 in hepatocytes and that of IGFBP-3 in KC, respectively. In cocultures, biosynthesis of IGFBP-1, -3 and -4 was increased dose-dependently by IL-6, while the effects of IL-1
β or TNF
α were less prominent. At neutral pH, proteolytic activity against IGFBP-3 was not detected in media of cocultures treated with IL-6.
Conclusions
: The alterations of IGF-I, IGFBP-1 and -4 observed in catabolism correlate with the effects of IL-6 on the biosynthesis of these components in primary rat liver cells, while a neutral IGFBP-3 protease was not detectable.</abstract><cop>Oxford</cop><pub>Elsevier B.V</pub><pmid>11690700</pmid><doi>10.1016/S0168-8278(01)00170-2</doi><tpages>10</tpages></addata></record> |
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| identifier | ISSN: 0168-8278 |
| ispartof | Journal of hepatology, 2001-11, Vol.35 (5), p.558-567 |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Biological and medical sciences Coculture Coculture Techniques DNA Probes Fundamental and applied biological sciences. Psychology Gene Expression Regulation - drug effects Gene Expression Regulation - physiology Hepatocytes Hepatocytes - cytology Hepatocytes - drug effects Hepatocytes - metabolism Insulin-Like Growth Factor Binding Protein 1 - genetics Insulin-Like Growth Factor Binding Protein 3 - genetics Insulin-Like Growth Factor Binding Protein 4 - genetics Insulin-like growth factor binding protein-3 protease Insulin-like growth factor binding proteins Insulin-Like Growth Factor I - genetics Insulin-like growth factor-I Interleukin-6 Interleukin-6 - pharmacology Kinetics Kupffer cells Kupffer Cells - cytology Kupffer Cells - drug effects Kupffer Cells - metabolism Liver. Bile. Biliary tracts Male Rats Rats, Wistar RNA, Messenger - genetics Transcription, Genetic - drug effects Vertebrates: digestive system |
| title | Regulation of insulin-like growth factor-I and of insulin-like growth factor binding protein-1, -3 and -4 in cocultures of rat hepatocytes and Kupffer cells by interleukin-6 |
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