Gene expression profiling: monitoring transcription and translation products using DNA microarrays and proteomics
Novel and powerful technologies such as DNA microarrays and proteomics have made possible the analysis of the expression levels of multiple genes simultaneously both in health and disease. In combination, these technologies promise to revolutionize biology, in particular in the area of molecular med...
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| Veröffentlicht in: | FEBS Letters 2000-08, Vol.480 (1), p.2-16 |
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| creator | Celis, Julio E Kruhøffer, Mogens Gromova, Irina Frederiksen, Casper Østergaard, Morten Thykjaer, Thomas Gromov, Pavel Yu, Jinsheng Pálsdóttir, Hildur Magnusson, Nils Ørntoft, Torben F |
| description | Novel and powerful technologies such as DNA microarrays and proteomics have made possible the analysis of the expression levels of multiple genes simultaneously both in health and disease. In combination, these technologies promise to revolutionize biology, in particular in the area of molecular medicine as they are expected to reveal gene regulation events involved in disease progression as well as to pinpoint potential targets for drug discovery and diagnostics. Here, we review the current status of these technologies and highlight some studies in which they have been applied in concert to the analysis of biopsy specimens. |
| doi_str_mv | 10.1016/S0014-5793(00)01771-3 |
| format | Article |
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In combination, these technologies promise to revolutionize biology, in particular in the area of molecular medicine as they are expected to reveal gene regulation events involved in disease progression as well as to pinpoint potential targets for drug discovery and diagnostics. 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Here, we review the current status of these technologies and highlight some studies in which they have been applied in concert to the analysis of biopsy specimens.</description><subject>2D PAGE, two-dimensional polyacrylamide gel electrophoresis</subject><subject>Animals</subject><subject>Biopsy</subject><subject>Bladder cancer</subject><subject>Computational Biology - methods</subject><subject>Databases as Topic</subject><subject>Disease Models, Animal</subject><subject>Electrophoresis, Gel, Two-Dimensional - methods</subject><subject>Gene expression profile</subject><subject>Gene Expression Profiling - methods</subject><subject>Humans</subject><subject>IEF, isoelectric focusing</subject><subject>Immunohistochemistry</subject><subject>IPG, immobilized pH gradient</subject><subject>Microarray</subject><subject>Neoplasms - genetics</subject><subject>Neoplasms - metabolism</subject><subject>Neoplasms - pathology</subject><subject>NEPHGE, non-equilibrium pH gradient electrophoresis</subject><subject>Oligonucleotide Array Sequence Analysis - methods</subject><subject>Proteins - analysis</subject><subject>Proteins - genetics</subject><subject>Proteome</subject><subject>Transcription, Genetic - genetics</subject><subject>Two-dimensional gel electrophoresis</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkctOwzAQRS0EglL4BFBWCBYBP-LYZYPKqyAhWMDecp0JMsoLOwH69zhJhdjByvbMuXdG1wgdEHxKMEnPnjEmSczFjB1jfIKJECRmG2hCpGAxS1K5iSY_yA7a9f4Nh7cks220Q_AsFYzSCXpfQAURfDUOvLd1FTWuzm1hq9fzqKwr29Yu3KPW6cobZ5u2Z3SVjZVCt2tN1pnWR53v4evHeVRa42rtnF75AQ9IC3Wo-j20levCw_76nKKX25uXq7v44WlxfzV_iA1nMxZTTgFSkwidkyWlOc04NbDkYXGhqZFCyAQzmgPPRcYlzrDUQHgOLIFAsik6Gm3D5PcOfKtK6w0Uha6g7rwSlCZSMhpAPoJhYe8d5KpxttRupQhWfdRqiFr1OSqM1RC1YkF3uB7QLUvIfqnGbANwNwKftoDV_1zV7c0lHTp9o_-wUO5nXYxWEAL7sOCUNxYqA5l1YFqV1faPbb8BoJikaw</recordid><startdate>20000825</startdate><enddate>20000825</enddate><creator>Celis, Julio E</creator><creator>Kruhøffer, Mogens</creator><creator>Gromova, Irina</creator><creator>Frederiksen, Casper</creator><creator>Østergaard, Morten</creator><creator>Thykjaer, Thomas</creator><creator>Gromov, Pavel</creator><creator>Yu, Jinsheng</creator><creator>Pálsdóttir, Hildur</creator><creator>Magnusson, Nils</creator><creator>Ørntoft, Torben F</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20000825</creationdate><title>Gene expression profiling: monitoring transcription and translation products using DNA microarrays and proteomics</title><author>Celis, Julio E ; 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| subjects | 2D PAGE, two-dimensional polyacrylamide gel electrophoresis Animals Biopsy Bladder cancer Computational Biology - methods Databases as Topic Disease Models, Animal Electrophoresis, Gel, Two-Dimensional - methods Gene expression profile Gene Expression Profiling - methods Humans IEF, isoelectric focusing Immunohistochemistry IPG, immobilized pH gradient Microarray Neoplasms - genetics Neoplasms - metabolism Neoplasms - pathology NEPHGE, non-equilibrium pH gradient electrophoresis Oligonucleotide Array Sequence Analysis - methods Proteins - analysis Proteins - genetics Proteome Transcription, Genetic - genetics Two-dimensional gel electrophoresis |
| title | Gene expression profiling: monitoring transcription and translation products using DNA microarrays and proteomics |
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