NADPH Supply and Mannitol Biosynthesis. Characterization, Cloning, and Regulation of the Non-Reversible Glyceraldehyde-3-Phosphate Dehydrogenase in Celery Leaves

Mannitol, a sugar alcohol, is a major primary photosynthetic product in celery (Apium graveolens L. cv Giant Pascal). We report here on purification, characterization, and cDNA cloning of cytosolic non-reversible glyceraldehyde-3-P dehydrogenase (nr-G3PDH, EC 1.2.1.9), the apparent key contributor o...

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Veröffentlicht in:	Plant physiology (Bethesda) 2000-09, Vol.124 (1), p.321-330
Hauptverfasser:	Gao, Z, Loescher, W H
Format:	Artikel
Sprache:	eng
Schlagworte:	Agronomy. Soil science and plant productions Amino Acid Sequence Amino acids Apiaceae - enzymology Apiaceae - genetics Apiaceae - metabolism Biological and medical sciences Biosynthesis Blotting, Northern Celery Chloroplasts Cloning Cloning, Molecular Complementary DNA Cytosol Dehydrogenase Dehydrogenases Economic plant physiology Electrophoresis, Polyacrylamide Gel Energy metabolism Environmental regulations Environmental Stress and Adaptation Enzymes Fractionation Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Plant Glyceraldehyde-3-Phosphate Dehydrogenases - genetics Glyceraldehyde-3-Phosphate Dehydrogenases - isolation & purification Glyceraldehyde-3-Phosphate Dehydrogenases - metabolism Leaves Mannitol - metabolism Metabolism Metabolites Molecular Sequence Data NADP - metabolism Nutrition. Photosynthesis. Respiration. Metabolism Plant Leaves - enzymology Plant Leaves - growth & development Plant Leaves - metabolism Plant physiology and development Plant Proteins - genetics Plant Proteins - isolation & purification Plant Proteins - metabolism Plants Polymerase Chain Reaction Sequence Alignment Sodium Chloride - metabolism Vegetables
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