Polyomavirus Enhancer-binding Protein 2/Core Binding Factor/Acute Myeloid Leukemia Factors Contribute to the Cell Type-specific Activity of the CD11a Integrin Gene Promoter
The CD11a/CD18 leukocyte integrin (LFA-1; also known as αL/β2) mediates leukocyte transendothelial migration during immune and inflammatory responses and participates in lymphoma metastasis. CD11a/CD18 leukocyte-restricted expression is controlled by the CD11a gene promoter, which confers tissue-s...
Gespeichert in:
| Veröffentlicht in: | The Journal of biological chemistry 2000-09, Vol.275 (37), p.28507-28512 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 28512 |
|---|---|
| container_issue | 37 |
| container_start_page | 28507 |
| container_title | The Journal of biological chemistry |
| container_volume | 275 |
| creator | Puig-Kroeger, A Lopez-Rodriguez, C Relloso, M Sanchez-Elsner, T Nueda, A Munoz, E Bernabeu, C Corbi, AL |
| description | The CD11a/CD18 leukocyte integrin (LFA-1; also known as αL/β2) mediates leukocyte transendothelial migration during immune
and inflammatory responses and participates in lymphoma metastasis. CD11a/CD18 leukocyte-restricted expression is controlled
by the CD11a gene promoter, which confers tissue-specific expression to reporter genes in vitro and in vivo . DNase I protection analysis of the CD11a proximal gene promoter revealed DNA-protein interactions centered at position â110 (CD11a-110). Disruption of CD11a-110 reduced CD11a promoter activity in a cell type-specific manner, as it reduced its activity by 70% in Jurkat lymphoid cells, whereas the
effect was considerably lower in K562 and HepG2 cells. Electrophoretic mobility shift assays showed evidence of cell type-specific
differences in CD11a-110 binding and indicated its specific recognition by members of the polyomavirus enhancer-binding protein
2/core binding factor (CBF)/acute myeloid leukemia (AML) family of transcription factors. AML1B/CBFβ transactivated the CD11a promoter, with AML1B/CBFβ-mediated transactivation being completely dependent on the integrity of the CD11a-110 element. Therefore,
CBF/AML factors play a role in the cell type-restricted transcription of the CD11a integrin gene through recognition of CD11a-110. The involvement of CBF/AML factors in CD11a expression raises the possibility
that CD11a/CD18 expression might be deregulated in acute myeloid and B-lineage acute lymphoblastic leukemias, thus contributing
to their altered adhesion and metastatic potential. |
| doi_str_mv | 10.1074/jbc.M004323200 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_72244215</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>72244215</sourcerecordid><originalsourceid>FETCH-LOGICAL-c457t-c4fded65bc0374884c9be589b5de9d36ca088b3fa8d425662385ce68f918efdc3</originalsourceid><addsrcrecordid>eNqFkU2P0zAQhiMEYsvClSPyAXFL68_YOZbsByt1xR4WiZuVOJPGSxMX21mU_8SPxFUqwQ0fxtLMMzPv6M2y9wSvCZZ889SY9T3GnFFGMX6RrQhWLGeCfH-ZrTCmJC-pUBfZmxCecHq8JK-ziwQpKhlbZb8f3GF2Q_1s_RTQ9djXowGfN3Zs7bhHD95FsCOim8p5QJ_P6ZvaROc3WzNFQPczHJxt0Q6mHzDY-lwNqHJj9LY5MdGh2AOq4HBAj_MR8nAEYztr0NZE-2zjjFy3IFeE1OhujLD3afEtjHBSMSQd_m32qqsPAd6d_8vs2831Y_Ul3329vau2u9xwIWOKXQttIRqDmeRKcVM2IFTZiBbKlhWmTuc3rKtVy6koCsqUMFCoriQKutawy-zTMvfo3c8JQtSDDSZpr0dwU9CSUs4pEf8FiSy4xIQkcL2AxrsQPHT66O1Q-1kTrE9G6mSk_mtkavhwnjw1A7T_4ItzCfi4AL3d97-sB91YZ3oYNJVCM6mpEliyP-pRp2k</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17647011</pqid></control><display><type>article</type><title>Polyomavirus Enhancer-binding Protein 2/Core Binding Factor/Acute Myeloid Leukemia Factors Contribute to the Cell Type-specific Activity of the CD11a Integrin Gene Promoter</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Alma/SFX Local Collection</source><creator>Puig-Kroeger, A ; Lopez-Rodriguez, C ; Relloso, M ; Sanchez-Elsner, T ; Nueda, A ; Munoz, E ; Bernabeu, C ; Corbi, AL</creator><creatorcontrib>Puig-Kroeger, A ; Lopez-Rodriguez, C ; Relloso, M ; Sanchez-Elsner, T ; Nueda, A ; Munoz, E ; Bernabeu, C ; Corbi, AL</creatorcontrib><description>The CD11a/CD18 leukocyte integrin (LFA-1; also known as αL/β2) mediates leukocyte transendothelial migration during immune
and inflammatory responses and participates in lymphoma metastasis. CD11a/CD18 leukocyte-restricted expression is controlled
by the CD11a gene promoter, which confers tissue-specific expression to reporter genes in vitro and in vivo . DNase I protection analysis of the CD11a proximal gene promoter revealed DNA-protein interactions centered at position â110 (CD11a-110). Disruption of CD11a-110 reduced CD11a promoter activity in a cell type-specific manner, as it reduced its activity by 70% in Jurkat lymphoid cells, whereas the
effect was considerably lower in K562 and HepG2 cells. Electrophoretic mobility shift assays showed evidence of cell type-specific
differences in CD11a-110 binding and indicated its specific recognition by members of the polyomavirus enhancer-binding protein
2/core binding factor (CBF)/acute myeloid leukemia (AML) family of transcription factors. AML1B/CBFβ transactivated the CD11a promoter, with AML1B/CBFβ-mediated transactivation being completely dependent on the integrity of the CD11a-110 element. Therefore,
CBF/AML factors play a role in the cell type-restricted transcription of the CD11a integrin gene through recognition of CD11a-110. The involvement of CBF/AML factors in CD11a expression raises the possibility
that CD11a/CD18 expression might be deregulated in acute myeloid and B-lineage acute lymphoblastic leukemias, thus contributing
to their altered adhesion and metastatic potential.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1074/jbc.M004323200</identifier><identifier>PMID: 10882733</identifier><language>eng</language><publisher>United States: American Society for Biochemistry and Molecular Biology</publisher><subject>AML1B protein ; CCAAT-Enhancer-Binding Proteins ; CD11a antigen ; Cell Line ; Core Binding Factor Alpha 2 Subunit ; core-binding factor ; DNA-Binding Proteins - physiology ; Humans ; Lymphocyte Function-Associated Antigen-1 - genetics ; Polyomavirus ; polyomavirus enhancer-binding protein 2 ; Promoter Regions, Genetic ; Proto-Oncogene Proteins ; Transcription Factor AP-2 ; Transcription Factors - physiology ; Transcriptional Activation</subject><ispartof>The Journal of biological chemistry, 2000-09, Vol.275 (37), p.28507-28512</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c457t-c4fded65bc0374884c9be589b5de9d36ca088b3fa8d425662385ce68f918efdc3</citedby><cites>FETCH-LOGICAL-c457t-c4fded65bc0374884c9be589b5de9d36ca088b3fa8d425662385ce68f918efdc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27913,27914</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10882733$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Puig-Kroeger, A</creatorcontrib><creatorcontrib>Lopez-Rodriguez, C</creatorcontrib><creatorcontrib>Relloso, M</creatorcontrib><creatorcontrib>Sanchez-Elsner, T</creatorcontrib><creatorcontrib>Nueda, A</creatorcontrib><creatorcontrib>Munoz, E</creatorcontrib><creatorcontrib>Bernabeu, C</creatorcontrib><creatorcontrib>Corbi, AL</creatorcontrib><title>Polyomavirus Enhancer-binding Protein 2/Core Binding Factor/Acute Myeloid Leukemia Factors Contribute to the Cell Type-specific Activity of the CD11a Integrin Gene Promoter</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>The CD11a/CD18 leukocyte integrin (LFA-1; also known as αL/β2) mediates leukocyte transendothelial migration during immune
and inflammatory responses and participates in lymphoma metastasis. CD11a/CD18 leukocyte-restricted expression is controlled
by the CD11a gene promoter, which confers tissue-specific expression to reporter genes in vitro and in vivo . DNase I protection analysis of the CD11a proximal gene promoter revealed DNA-protein interactions centered at position â110 (CD11a-110). Disruption of CD11a-110 reduced CD11a promoter activity in a cell type-specific manner, as it reduced its activity by 70% in Jurkat lymphoid cells, whereas the
effect was considerably lower in K562 and HepG2 cells. Electrophoretic mobility shift assays showed evidence of cell type-specific
differences in CD11a-110 binding and indicated its specific recognition by members of the polyomavirus enhancer-binding protein
2/core binding factor (CBF)/acute myeloid leukemia (AML) family of transcription factors. AML1B/CBFβ transactivated the CD11a promoter, with AML1B/CBFβ-mediated transactivation being completely dependent on the integrity of the CD11a-110 element. Therefore,
CBF/AML factors play a role in the cell type-restricted transcription of the CD11a integrin gene through recognition of CD11a-110. The involvement of CBF/AML factors in CD11a expression raises the possibility
that CD11a/CD18 expression might be deregulated in acute myeloid and B-lineage acute lymphoblastic leukemias, thus contributing
to their altered adhesion and metastatic potential.</description><subject>AML1B protein</subject><subject>CCAAT-Enhancer-Binding Proteins</subject><subject>CD11a antigen</subject><subject>Cell Line</subject><subject>Core Binding Factor Alpha 2 Subunit</subject><subject>core-binding factor</subject><subject>DNA-Binding Proteins - physiology</subject><subject>Humans</subject><subject>Lymphocyte Function-Associated Antigen-1 - genetics</subject><subject>Polyomavirus</subject><subject>polyomavirus enhancer-binding protein 2</subject><subject>Promoter Regions, Genetic</subject><subject>Proto-Oncogene Proteins</subject><subject>Transcription Factor AP-2</subject><subject>Transcription Factors - physiology</subject><subject>Transcriptional Activation</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU2P0zAQhiMEYsvClSPyAXFL68_YOZbsByt1xR4WiZuVOJPGSxMX21mU_8SPxFUqwQ0fxtLMMzPv6M2y9wSvCZZ889SY9T3GnFFGMX6RrQhWLGeCfH-ZrTCmJC-pUBfZmxCecHq8JK-ziwQpKhlbZb8f3GF2Q_1s_RTQ9djXowGfN3Zs7bhHD95FsCOim8p5QJ_P6ZvaROc3WzNFQPczHJxt0Q6mHzDY-lwNqHJj9LY5MdGh2AOq4HBAj_MR8nAEYztr0NZE-2zjjFy3IFeE1OhujLD3afEtjHBSMSQd_m32qqsPAd6d_8vs2831Y_Ul3329vau2u9xwIWOKXQttIRqDmeRKcVM2IFTZiBbKlhWmTuc3rKtVy6koCsqUMFCoriQKutawy-zTMvfo3c8JQtSDDSZpr0dwU9CSUs4pEf8FiSy4xIQkcL2AxrsQPHT66O1Q-1kTrE9G6mSk_mtkavhwnjw1A7T_4ItzCfi4AL3d97-sB91YZ3oYNJVCM6mpEliyP-pRp2k</recordid><startdate>20000915</startdate><enddate>20000915</enddate><creator>Puig-Kroeger, A</creator><creator>Lopez-Rodriguez, C</creator><creator>Relloso, M</creator><creator>Sanchez-Elsner, T</creator><creator>Nueda, A</creator><creator>Munoz, E</creator><creator>Bernabeu, C</creator><creator>Corbi, AL</creator><general>American Society for Biochemistry and Molecular Biology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20000915</creationdate><title>Polyomavirus Enhancer-binding Protein 2/Core Binding Factor/Acute Myeloid Leukemia Factors Contribute to the Cell Type-specific Activity of the CD11a Integrin Gene Promoter</title><author>Puig-Kroeger, A ; Lopez-Rodriguez, C ; Relloso, M ; Sanchez-Elsner, T ; Nueda, A ; Munoz, E ; Bernabeu, C ; Corbi, AL</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c457t-c4fded65bc0374884c9be589b5de9d36ca088b3fa8d425662385ce68f918efdc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>AML1B protein</topic><topic>CCAAT-Enhancer-Binding Proteins</topic><topic>CD11a antigen</topic><topic>Cell Line</topic><topic>Core Binding Factor Alpha 2 Subunit</topic><topic>core-binding factor</topic><topic>DNA-Binding Proteins - physiology</topic><topic>Humans</topic><topic>Lymphocyte Function-Associated Antigen-1 - genetics</topic><topic>Polyomavirus</topic><topic>polyomavirus enhancer-binding protein 2</topic><topic>Promoter Regions, Genetic</topic><topic>Proto-Oncogene Proteins</topic><topic>Transcription Factor AP-2</topic><topic>Transcription Factors - physiology</topic><topic>Transcriptional Activation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Puig-Kroeger, A</creatorcontrib><creatorcontrib>Lopez-Rodriguez, C</creatorcontrib><creatorcontrib>Relloso, M</creatorcontrib><creatorcontrib>Sanchez-Elsner, T</creatorcontrib><creatorcontrib>Nueda, A</creatorcontrib><creatorcontrib>Munoz, E</creatorcontrib><creatorcontrib>Bernabeu, C</creatorcontrib><creatorcontrib>Corbi, AL</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Puig-Kroeger, A</au><au>Lopez-Rodriguez, C</au><au>Relloso, M</au><au>Sanchez-Elsner, T</au><au>Nueda, A</au><au>Munoz, E</au><au>Bernabeu, C</au><au>Corbi, AL</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Polyomavirus Enhancer-binding Protein 2/Core Binding Factor/Acute Myeloid Leukemia Factors Contribute to the Cell Type-specific Activity of the CD11a Integrin Gene Promoter</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>2000-09-15</date><risdate>2000</risdate><volume>275</volume><issue>37</issue><spage>28507</spage><epage>28512</epage><pages>28507-28512</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><abstract>The CD11a/CD18 leukocyte integrin (LFA-1; also known as αL/β2) mediates leukocyte transendothelial migration during immune
and inflammatory responses and participates in lymphoma metastasis. CD11a/CD18 leukocyte-restricted expression is controlled
by the CD11a gene promoter, which confers tissue-specific expression to reporter genes in vitro and in vivo . DNase I protection analysis of the CD11a proximal gene promoter revealed DNA-protein interactions centered at position â110 (CD11a-110). Disruption of CD11a-110 reduced CD11a promoter activity in a cell type-specific manner, as it reduced its activity by 70% in Jurkat lymphoid cells, whereas the
effect was considerably lower in K562 and HepG2 cells. Electrophoretic mobility shift assays showed evidence of cell type-specific
differences in CD11a-110 binding and indicated its specific recognition by members of the polyomavirus enhancer-binding protein
2/core binding factor (CBF)/acute myeloid leukemia (AML) family of transcription factors. AML1B/CBFβ transactivated the CD11a promoter, with AML1B/CBFβ-mediated transactivation being completely dependent on the integrity of the CD11a-110 element. Therefore,
CBF/AML factors play a role in the cell type-restricted transcription of the CD11a integrin gene through recognition of CD11a-110. The involvement of CBF/AML factors in CD11a expression raises the possibility
that CD11a/CD18 expression might be deregulated in acute myeloid and B-lineage acute lymphoblastic leukemias, thus contributing
to their altered adhesion and metastatic potential.</abstract><cop>United States</cop><pub>American Society for Biochemistry and Molecular Biology</pub><pmid>10882733</pmid><doi>10.1074/jbc.M004323200</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-9258 |
| ispartof | The Journal of biological chemistry, 2000-09, Vol.275 (37), p.28507-28512 |
| issn | 0021-9258 1083-351X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_72244215 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | AML1B protein CCAAT-Enhancer-Binding Proteins CD11a antigen Cell Line Core Binding Factor Alpha 2 Subunit core-binding factor DNA-Binding Proteins - physiology Humans Lymphocyte Function-Associated Antigen-1 - genetics Polyomavirus polyomavirus enhancer-binding protein 2 Promoter Regions, Genetic Proto-Oncogene Proteins Transcription Factor AP-2 Transcription Factors - physiology Transcriptional Activation |
| title | Polyomavirus Enhancer-binding Protein 2/Core Binding Factor/Acute Myeloid Leukemia Factors Contribute to the Cell Type-specific Activity of the CD11a Integrin Gene Promoter |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-15T08%3A28%3A56IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Polyomavirus%20Enhancer-binding%20Protein%202/Core%20Binding%20Factor/Acute%20Myeloid%20Leukemia%20Factors%20Contribute%20to%20the%20Cell%20Type-specific%20Activity%20of%20the%20CD11a%20Integrin%20Gene%20Promoter&rft.jtitle=The%20Journal%20of%20biological%20chemistry&rft.au=Puig-Kroeger,%20A&rft.date=2000-09-15&rft.volume=275&rft.issue=37&rft.spage=28507&rft.epage=28512&rft.pages=28507-28512&rft.issn=0021-9258&rft.eissn=1083-351X&rft_id=info:doi/10.1074/jbc.M004323200&rft_dat=%3Cproquest_cross%3E72244215%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=17647011&rft_id=info:pmid/10882733&rfr_iscdi=true |