Sensitive and specific enzyme-linked immunosorbent assay for the diagnosis of Wuchereria bancrofti infection in urine samples
We developed an enzyme-linked immunosorbent assay (ELISA) that detects filaria-specific immunoglobulin G4 antibodies in unconcentrated urine. The ELISA was positive in 87 of 91 (95.6%) urine samples collected from people with Wuchereria bancrofti microfilariae, antigen, or both. Of 298 urine samples...
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| Veröffentlicht in: | The American journal of tropical medicine and hygiene 2001-10, Vol.65 (4), p.362-365 |
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| container_title | The American journal of tropical medicine and hygiene |
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| creator | Itoh, M Weerasooriya, MV Qiu, G Gunawardena, NK Anantaphruti, MT Tesana, S Rattanaxay, P Fujimaki, Y Kimura, E |
| description | We developed an enzyme-linked immunosorbent assay (ELISA) that detects filaria-specific immunoglobulin G4 antibodies in unconcentrated urine. The ELISA was positive in 87 of 91 (95.6%) urine samples collected from people with Wuchereria bancrofti microfilariae, antigen, or both. Of 298 urine samples collected in Thailand, Lao People's Democratic Republic, and Japan, where no human filariasis is known, 295 (99.0%) were negative by ELISA. Various intestinal nematode and fluke infections did not interfere with the ELISA. Urine samples with sodium azide could be kept at 37 degrees C for 4 weeks, and the time of urine collection did not influence ELISA results. This ELISA can be used to identify endemic foci of filariasis. |
| doi_str_mv | 10.4269/ajtmh.2001.65.362 |
| format | Article |
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The ELISA was positive in 87 of 91 (95.6%) urine samples collected from people with Wuchereria bancrofti microfilariae, antigen, or both. Of 298 urine samples collected in Thailand, Lao People's Democratic Republic, and Japan, where no human filariasis is known, 295 (99.0%) were negative by ELISA. Various intestinal nematode and fluke infections did not interfere with the ELISA. Urine samples with sodium azide could be kept at 37 degrees C for 4 weeks, and the time of urine collection did not influence ELISA results. 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The ELISA was positive in 87 of 91 (95.6%) urine samples collected from people with Wuchereria bancrofti microfilariae, antigen, or both. Of 298 urine samples collected in Thailand, Lao People's Democratic Republic, and Japan, where no human filariasis is known, 295 (99.0%) were negative by ELISA. Various intestinal nematode and fluke infections did not interfere with the ELISA. Urine samples with sodium azide could be kept at 37 degrees C for 4 weeks, and the time of urine collection did not influence ELISA results. This ELISA can be used to identify endemic foci of filariasis.</description><subject>Animals</subject><subject>Antibodies, Helminth - urine</subject><subject>Antigens, Helminth - analysis</subject><subject>Biological and medical sciences</subject><subject>Diseases caused by nematodes</subject><subject>Elephantiasis, Filarial - diagnosis</subject><subject>Elephantiasis, Filarial - urine</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Filariases</subject><subject>Helminthic diseases</subject><subject>Humans</subject><subject>Immunoglobulin G - urine</subject><subject>Infectious diseases</subject><subject>Lymphatic filariases</subject><subject>Medical sciences</subject><subject>Parasitic diseases</subject><subject>Sensitivity and Specificity</subject><subject>Time Factors</subject><subject>Tropical medicine</subject><subject>Wuchereria bancrofti - immunology</subject><issn>0002-9637</issn><issn>1476-1645</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkMFu1DAQhi0EokvhAbggX-CWxXZsJzmiqlCkShwAcbQce9y4JM7iSbpaJN4db7vSnmak-eYf-yPkLWdbKXT30d4v07AVjPGtVttai2dkw2WjK66lek42jDFRdbpuLsgrxPvCtYLzl-SCc93Vbas25N93SBiX-ADUJk9xBy6G6Cikv4cJqjGm3-BpnKY1zTjnHtJCLaI90DBnugxAfbR3ZRaRzoH-Wt0AGXK0tLfJ5TkskcYUwC1xTqWja44JKNppNwK-Ji-CHRHenOol-fn5-sfVTXX77cvXq0-3lZNaL5X0jRSOKa15L1wnePDQWcWUb5u6Bl8-JXorvdLedaxv4TiywvIamtBZX1-SD0-5uzz_WQEXM0V0MI42wbyiaYSQtRKigPwJLE9HzBDMLsfJ5oPhzBydm0fn5ujcaGWK87Lz7hS-9hP488ZJcgHenwCLzo4hFzMRz5wsZK31mRvi3bCPGQxOdhxLLDf7_b6ck48H_wPmZZsZ</recordid><startdate>20011001</startdate><enddate>20011001</enddate><creator>Itoh, M</creator><creator>Weerasooriya, MV</creator><creator>Qiu, G</creator><creator>Gunawardena, NK</creator><creator>Anantaphruti, MT</creator><creator>Tesana, S</creator><creator>Rattanaxay, P</creator><creator>Fujimaki, Y</creator><creator>Kimura, E</creator><general>ASTMH</general><general>Allen Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20011001</creationdate><title>Sensitive and specific enzyme-linked immunosorbent assay for the diagnosis of Wuchereria bancrofti infection in urine samples</title><author>Itoh, M ; Weerasooriya, MV ; Qiu, G ; Gunawardena, NK ; Anantaphruti, MT ; Tesana, S ; Rattanaxay, P ; Fujimaki, Y ; Kimura, E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c466t-4d742c05661b2c921fde9a505d8733ed2112ba4d56dc90b8e505da2a13e7f9ad3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Animals</topic><topic>Antibodies, Helminth - urine</topic><topic>Antigens, Helminth - analysis</topic><topic>Biological and medical sciences</topic><topic>Diseases caused by nematodes</topic><topic>Elephantiasis, Filarial - diagnosis</topic><topic>Elephantiasis, Filarial - urine</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Filariases</topic><topic>Helminthic diseases</topic><topic>Humans</topic><topic>Immunoglobulin G - urine</topic><topic>Infectious diseases</topic><topic>Lymphatic filariases</topic><topic>Medical sciences</topic><topic>Parasitic diseases</topic><topic>Sensitivity and Specificity</topic><topic>Time Factors</topic><topic>Tropical medicine</topic><topic>Wuchereria bancrofti - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Itoh, M</creatorcontrib><creatorcontrib>Weerasooriya, MV</creatorcontrib><creatorcontrib>Qiu, G</creatorcontrib><creatorcontrib>Gunawardena, NK</creatorcontrib><creatorcontrib>Anantaphruti, MT</creatorcontrib><creatorcontrib>Tesana, S</creatorcontrib><creatorcontrib>Rattanaxay, P</creatorcontrib><creatorcontrib>Fujimaki, Y</creatorcontrib><creatorcontrib>Kimura, E</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The American journal of tropical medicine and hygiene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Itoh, M</au><au>Weerasooriya, MV</au><au>Qiu, G</au><au>Gunawardena, NK</au><au>Anantaphruti, MT</au><au>Tesana, S</au><au>Rattanaxay, P</au><au>Fujimaki, Y</au><au>Kimura, E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sensitive and specific enzyme-linked immunosorbent assay for the diagnosis of Wuchereria bancrofti infection in urine samples</atitle><jtitle>The American journal of tropical medicine and hygiene</jtitle><addtitle>Am J Trop Med Hyg</addtitle><date>2001-10-01</date><risdate>2001</risdate><volume>65</volume><issue>4</issue><spage>362</spage><epage>365</epage><pages>362-365</pages><issn>0002-9637</issn><eissn>1476-1645</eissn><coden>AJTHAB</coden><abstract>We developed an enzyme-linked immunosorbent assay (ELISA) that detects filaria-specific immunoglobulin G4 antibodies in unconcentrated urine. 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| subjects | Animals Antibodies, Helminth - urine Antigens, Helminth - analysis Biological and medical sciences Diseases caused by nematodes Elephantiasis, Filarial - diagnosis Elephantiasis, Filarial - urine Enzyme-Linked Immunosorbent Assay - methods Filariases Helminthic diseases Humans Immunoglobulin G - urine Infectious diseases Lymphatic filariases Medical sciences Parasitic diseases Sensitivity and Specificity Time Factors Tropical medicine Wuchereria bancrofti - immunology |
| title | Sensitive and specific enzyme-linked immunosorbent assay for the diagnosis of Wuchereria bancrofti infection in urine samples |
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