Directed evolution: the ‘rational’ basis for ‘irrational’ design
The development of powerful genetic manipulation formats has revolutionized the creation of functional biological molecules. Recent advances in directed evolution demonstrate that multiple properties of proteins can be optimized simultaneously and rapidly. Improved proteins often contain multiple an...
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| Veröffentlicht in: | Current Opinion in Structural Biology 2000-08, Vol.10 (4), p.421-427 |
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| container_end_page | 427 |
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| container_title | Current Opinion in Structural Biology |
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| creator | Tobin, Matthew B Gustafsson, Claes Huisman, Gjalt W |
| description | The development of powerful genetic manipulation formats has revolutionized the creation of functional biological molecules. Recent advances in directed evolution demonstrate that multiple properties of proteins can be optimized simultaneously and rapidly. Improved proteins often contain multiple and dispersed substitutions that act synergistically to improve enzyme properties and function. The benefits of such multiple changes are often not predictable from
a priori structural knowledge. Furthermore, alternative solutions to gaining functional change can be obtained. |
| doi_str_mv | 10.1016/S0959-440X(00)00109-3 |
| format | Article |
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| ispartof | Current Opinion in Structural Biology, 2000-08, Vol.10 (4), p.421-427 |
| issn | 0959-440X 1879-033X |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals directed evolution Directed molecular evolution Drug Design Gene shuffling Humans Mutagenesis Protein Engineering Proteins - chemistry Proteins - genetics Recombination Sequence space Structure-Activity Relationship |
| title | Directed evolution: the ‘rational’ basis for ‘irrational’ design |
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