Genomic organization, chromosomal localization and regulation of expression of the neuronal nuclear matrix protein NRP/B in human brain tumors
The nuclear matrix and its role in cell physiology are largely unknown, and the discovery of any matrix constituent whose expression is tissue- and/or cell-specific offers a new avenue of exploration. Studies of the novel neuronal nuclear matrix protein, NRP/B, reveal that it is an early and highly...
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| Veröffentlicht in: | Gene 2000-09, Vol.255 (1), p.105-116 |
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| creator | Kim, Tae-Aug Ota, Setsuo Jiang, Shuxian Pasztor, Linda M. White, Robert A. Avraham, Shalom |
| description | The nuclear matrix and its role in cell physiology are largely unknown, and the discovery of any matrix constituent whose expression is tissue- and/or cell-specific offers a new avenue of exploration. Studies of the novel neuronal nuclear matrix protein, NRP/B, reveal that it is an early and highly specific marker of neuronal induction and development in vertebrates, since its expression is restricted mainly to the developing and mature nervous system. These studies also show that NRP/B is involved in neuronal differentiation. To further examine the structure–function of NRP/B, we have cloned and characterized the murine
Nrp/b gene. The murine gene consists of four exons interrupted by three introns that span 7.6
kb of DNA. The complete open reading frame is localized in exon 3, suggesting that
NRP/B is highly conserved during evolution. Chromosomal analysis shows that
NRP/B is localized to chromosome 13 in mouse and chromosome 5q12–13 in human.
Since our previous studies demonstrated that NRP/B is expressed in primary hippocampal neurons but not in primary astrocytes, we have characterized
NRP/B mRNA and protein expression in various brain cell lines and in human brain tumors. Abundant expression of
NRP/B mRNA and protein was observed in human neuroblastoma cell lines (IMR32, SKN-MC, SKN-SH), in glioblastoma cell lines (A172, T98G, U87-MG, U118-MG, U138-MG, and U373-MG), in neuroglioma (H4) and astrocytoma cell lines (CCF-STTG1 and SW1088). Confocal analysis of
NRP/B in U87-MG glioblastoma cells indicated nuclear localization of
NRP/B.
NRP/B expression was also observed in human primary brain tumors including glioblastoma multiformae and astrocytomas (total of five cases). These results suggest that NRP/B expression is upregulated in human brain tumors including glioblastomas and astrocytomas, while under normal conditions NRP/B expression is restricted to neurons. This study implicates a role for NRP/B in brain tumor development. |
| doi_str_mv | 10.1016/S0378-1119(00)00297-3 |
| format | Article |
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Nrp/b gene. The murine gene consists of four exons interrupted by three introns that span 7.6
kb of DNA. The complete open reading frame is localized in exon 3, suggesting that
NRP/B is highly conserved during evolution. Chromosomal analysis shows that
NRP/B is localized to chromosome 13 in mouse and chromosome 5q12–13 in human.
Since our previous studies demonstrated that NRP/B is expressed in primary hippocampal neurons but not in primary astrocytes, we have characterized
NRP/B mRNA and protein expression in various brain cell lines and in human brain tumors. Abundant expression of
NRP/B mRNA and protein was observed in human neuroblastoma cell lines (IMR32, SKN-MC, SKN-SH), in glioblastoma cell lines (A172, T98G, U87-MG, U118-MG, U138-MG, and U373-MG), in neuroglioma (H4) and astrocytoma cell lines (CCF-STTG1 and SW1088). Confocal analysis of
NRP/B in U87-MG glioblastoma cells indicated nuclear localization of
NRP/B.
NRP/B expression was also observed in human primary brain tumors including glioblastoma multiformae and astrocytomas (total of five cases). These results suggest that NRP/B expression is upregulated in human brain tumors including glioblastomas and astrocytomas, while under normal conditions NRP/B expression is restricted to neurons. This study implicates a role for NRP/B in brain tumor development.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/S0378-1119(00)00297-3</identifier><identifier>PMID: 10974570</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; astrocytoma ; Brain Neoplasms - genetics ; Brain Neoplasms - pathology ; BTB/POZ domain ; Cell Line ; Cells, Cultured ; chromosome 13 ; chromosome 5 ; Chromosome 5q ; Chromosome Mapping ; Chromosomes, Human, Pair 5 - genetics ; Cloning, Molecular ; Cricetinae ; DNA - chemistry ; DNA - genetics ; Exons ; Female ; Gene Expression ; Gene Expression Regulation, Neoplastic ; Genes - genetics ; glioblastoma ; Human brain tumor ; Humans ; Hybrid Cells ; Introns ; Kelch motif ; Male ; Mice ; Mice, Inbred C57BL ; Microfilament Proteins - genetics ; Microscopy, Confocal ; Muridae ; Neurons - chemistry ; Neurons - cytology ; Neuropeptides - genetics ; Nrp/b gene ; NRP/B protein ; Nuclear matrix protein ; Nuclear Proteins - genetics ; Rats ; Rats, Sprague-Dawley ; RNA - genetics ; RNA - metabolism ; Sequence Analysis, DNA ; Transcription, Genetic ; Tumor Cells, Cultured</subject><ispartof>Gene, 2000-09, Vol.255 (1), p.105-116</ispartof><rights>2000 Elsevier Science B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c444t-7cd74e32cb466bcd15a0a61698357d1ca23c7ab62528e1bb545aea72bff72a4f3</citedby><cites>FETCH-LOGICAL-c444t-7cd74e32cb466bcd15a0a61698357d1ca23c7ab62528e1bb545aea72bff72a4f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0378111900002973$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10974570$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Tae-Aug</creatorcontrib><creatorcontrib>Ota, Setsuo</creatorcontrib><creatorcontrib>Jiang, Shuxian</creatorcontrib><creatorcontrib>Pasztor, Linda M.</creatorcontrib><creatorcontrib>White, Robert A.</creatorcontrib><creatorcontrib>Avraham, Shalom</creatorcontrib><title>Genomic organization, chromosomal localization and regulation of expression of the neuronal nuclear matrix protein NRP/B in human brain tumors</title><title>Gene</title><addtitle>Gene</addtitle><description>The nuclear matrix and its role in cell physiology are largely unknown, and the discovery of any matrix constituent whose expression is tissue- and/or cell-specific offers a new avenue of exploration. Studies of the novel neuronal nuclear matrix protein, NRP/B, reveal that it is an early and highly specific marker of neuronal induction and development in vertebrates, since its expression is restricted mainly to the developing and mature nervous system. These studies also show that NRP/B is involved in neuronal differentiation. To further examine the structure–function of NRP/B, we have cloned and characterized the murine
Nrp/b gene. The murine gene consists of four exons interrupted by three introns that span 7.6
kb of DNA. The complete open reading frame is localized in exon 3, suggesting that
NRP/B is highly conserved during evolution. Chromosomal analysis shows that
NRP/B is localized to chromosome 13 in mouse and chromosome 5q12–13 in human.
Since our previous studies demonstrated that NRP/B is expressed in primary hippocampal neurons but not in primary astrocytes, we have characterized
NRP/B mRNA and protein expression in various brain cell lines and in human brain tumors. Abundant expression of
NRP/B mRNA and protein was observed in human neuroblastoma cell lines (IMR32, SKN-MC, SKN-SH), in glioblastoma cell lines (A172, T98G, U87-MG, U118-MG, U138-MG, and U373-MG), in neuroglioma (H4) and astrocytoma cell lines (CCF-STTG1 and SW1088). Confocal analysis of
NRP/B in U87-MG glioblastoma cells indicated nuclear localization of
NRP/B.
NRP/B expression was also observed in human primary brain tumors including glioblastoma multiformae and astrocytomas (total of five cases). These results suggest that NRP/B expression is upregulated in human brain tumors including glioblastomas and astrocytomas, while under normal conditions NRP/B expression is restricted to neurons. This study implicates a role for NRP/B in brain tumor development.</description><subject>Animals</subject><subject>astrocytoma</subject><subject>Brain Neoplasms - genetics</subject><subject>Brain Neoplasms - pathology</subject><subject>BTB/POZ domain</subject><subject>Cell Line</subject><subject>Cells, Cultured</subject><subject>chromosome 13</subject><subject>chromosome 5</subject><subject>Chromosome 5q</subject><subject>Chromosome Mapping</subject><subject>Chromosomes, Human, Pair 5 - genetics</subject><subject>Cloning, Molecular</subject><subject>Cricetinae</subject><subject>DNA - chemistry</subject><subject>DNA - genetics</subject><subject>Exons</subject><subject>Female</subject><subject>Gene Expression</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Genes - genetics</subject><subject>glioblastoma</subject><subject>Human brain tumor</subject><subject>Humans</subject><subject>Hybrid Cells</subject><subject>Introns</subject><subject>Kelch motif</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Microfilament Proteins - genetics</subject><subject>Microscopy, Confocal</subject><subject>Muridae</subject><subject>Neurons - chemistry</subject><subject>Neurons - cytology</subject><subject>Neuropeptides - genetics</subject><subject>Nrp/b gene</subject><subject>NRP/B protein</subject><subject>Nuclear matrix protein</subject><subject>Nuclear Proteins - genetics</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>RNA - genetics</subject><subject>RNA - metabolism</subject><subject>Sequence Analysis, DNA</subject><subject>Transcription, Genetic</subject><subject>Tumor Cells, Cultured</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctu1TAQhi0EoofCI4C8QiAR6ruTFYIKSqUKEJe1NXEmPUaJfWonqO1D8MykzRHqrt54Rv5-j-WPkOecveWMm6MfTNq64pw3rxh7zZhobCUfkA2vbVMxJuuHZPMfOSBPSvnNlqW1eEwOOGus0pZtyN8TjGkMnqZ8DjFcwxRSfEP9NqcxlTTCQIfkYdifUIgdzXg-D2ubeoqXu4yl7LtpizTinFNcknH2A0KmI0w5XNJdThOGSL98_3b0gS7Fdh4h0jbDUk_zmHJ5Sh71MBR8tt8Pya9PH38ef67Ovp6cHr8_q7xSaqqs76xCKXyrjGl9xzUwMNw0tdS24x6E9BZaI7SokbetVhoQrGj73gpQvTwkL9d7lzddzFgmN4bicRggYpqLs0IoXmt7L8it4ZIbs4B6BX1OpWTs3S6HEfKV48zdGHO3xtyNDseYuzXm5JJ7sR8wtyN2d1KrogV4twK4_MefgNkVHzB67EJGP7kuhXtG_AN246ja</recordid><startdate>20000905</startdate><enddate>20000905</enddate><creator>Kim, Tae-Aug</creator><creator>Ota, Setsuo</creator><creator>Jiang, Shuxian</creator><creator>Pasztor, Linda M.</creator><creator>White, Robert A.</creator><creator>Avraham, Shalom</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20000905</creationdate><title>Genomic organization, chromosomal localization and regulation of expression of the neuronal nuclear matrix protein NRP/B in human brain tumors</title><author>Kim, Tae-Aug ; Ota, Setsuo ; Jiang, Shuxian ; Pasztor, Linda M. ; White, Robert A. ; Avraham, Shalom</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c444t-7cd74e32cb466bcd15a0a61698357d1ca23c7ab62528e1bb545aea72bff72a4f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>astrocytoma</topic><topic>Brain Neoplasms - genetics</topic><topic>Brain Neoplasms - pathology</topic><topic>BTB/POZ domain</topic><topic>Cell Line</topic><topic>Cells, Cultured</topic><topic>chromosome 13</topic><topic>chromosome 5</topic><topic>Chromosome 5q</topic><topic>Chromosome Mapping</topic><topic>Chromosomes, Human, Pair 5 - genetics</topic><topic>Cloning, Molecular</topic><topic>Cricetinae</topic><topic>DNA - chemistry</topic><topic>DNA - genetics</topic><topic>Exons</topic><topic>Female</topic><topic>Gene Expression</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Genes - genetics</topic><topic>glioblastoma</topic><topic>Human brain tumor</topic><topic>Humans</topic><topic>Hybrid Cells</topic><topic>Introns</topic><topic>Kelch motif</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Microfilament Proteins - genetics</topic><topic>Microscopy, Confocal</topic><topic>Muridae</topic><topic>Neurons - chemistry</topic><topic>Neurons - cytology</topic><topic>Neuropeptides - genetics</topic><topic>Nrp/b gene</topic><topic>NRP/B protein</topic><topic>Nuclear matrix protein</topic><topic>Nuclear Proteins - genetics</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>RNA - genetics</topic><topic>RNA - metabolism</topic><topic>Sequence Analysis, DNA</topic><topic>Transcription, Genetic</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Tae-Aug</creatorcontrib><creatorcontrib>Ota, Setsuo</creatorcontrib><creatorcontrib>Jiang, Shuxian</creatorcontrib><creatorcontrib>Pasztor, Linda M.</creatorcontrib><creatorcontrib>White, Robert A.</creatorcontrib><creatorcontrib>Avraham, Shalom</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Tae-Aug</au><au>Ota, Setsuo</au><au>Jiang, Shuxian</au><au>Pasztor, Linda M.</au><au>White, Robert A.</au><au>Avraham, Shalom</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genomic organization, chromosomal localization and regulation of expression of the neuronal nuclear matrix protein NRP/B in human brain tumors</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>2000-09-05</date><risdate>2000</risdate><volume>255</volume><issue>1</issue><spage>105</spage><epage>116</epage><pages>105-116</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><abstract>The nuclear matrix and its role in cell physiology are largely unknown, and the discovery of any matrix constituent whose expression is tissue- and/or cell-specific offers a new avenue of exploration. Studies of the novel neuronal nuclear matrix protein, NRP/B, reveal that it is an early and highly specific marker of neuronal induction and development in vertebrates, since its expression is restricted mainly to the developing and mature nervous system. These studies also show that NRP/B is involved in neuronal differentiation. To further examine the structure–function of NRP/B, we have cloned and characterized the murine
Nrp/b gene. The murine gene consists of four exons interrupted by three introns that span 7.6
kb of DNA. The complete open reading frame is localized in exon 3, suggesting that
NRP/B is highly conserved during evolution. Chromosomal analysis shows that
NRP/B is localized to chromosome 13 in mouse and chromosome 5q12–13 in human.
Since our previous studies demonstrated that NRP/B is expressed in primary hippocampal neurons but not in primary astrocytes, we have characterized
NRP/B mRNA and protein expression in various brain cell lines and in human brain tumors. Abundant expression of
NRP/B mRNA and protein was observed in human neuroblastoma cell lines (IMR32, SKN-MC, SKN-SH), in glioblastoma cell lines (A172, T98G, U87-MG, U118-MG, U138-MG, and U373-MG), in neuroglioma (H4) and astrocytoma cell lines (CCF-STTG1 and SW1088). Confocal analysis of
NRP/B in U87-MG glioblastoma cells indicated nuclear localization of
NRP/B.
NRP/B expression was also observed in human primary brain tumors including glioblastoma multiformae and astrocytomas (total of five cases). These results suggest that NRP/B expression is upregulated in human brain tumors including glioblastomas and astrocytomas, while under normal conditions NRP/B expression is restricted to neurons. This study implicates a role for NRP/B in brain tumor development.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>10974570</pmid><doi>10.1016/S0378-1119(00)00297-3</doi><tpages>12</tpages></addata></record> |
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| ispartof | Gene, 2000-09, Vol.255 (1), p.105-116 |
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| subjects | Animals astrocytoma Brain Neoplasms - genetics Brain Neoplasms - pathology BTB/POZ domain Cell Line Cells, Cultured chromosome 13 chromosome 5 Chromosome 5q Chromosome Mapping Chromosomes, Human, Pair 5 - genetics Cloning, Molecular Cricetinae DNA - chemistry DNA - genetics Exons Female Gene Expression Gene Expression Regulation, Neoplastic Genes - genetics glioblastoma Human brain tumor Humans Hybrid Cells Introns Kelch motif Male Mice Mice, Inbred C57BL Microfilament Proteins - genetics Microscopy, Confocal Muridae Neurons - chemistry Neurons - cytology Neuropeptides - genetics Nrp/b gene NRP/B protein Nuclear matrix protein Nuclear Proteins - genetics Rats Rats, Sprague-Dawley RNA - genetics RNA - metabolism Sequence Analysis, DNA Transcription, Genetic Tumor Cells, Cultured |
| title | Genomic organization, chromosomal localization and regulation of expression of the neuronal nuclear matrix protein NRP/B in human brain tumors |
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