Multivesicular Release at Climbing Fiber-Purkinje Cell Synapses
Synapses driven by action potentials are thought to release transmitter in an all-or-none fashion; either one synaptic vesicle undergoes exocytosis, or there is no release. We have estimated the glutamate concentration transient at climbing fiber synapses on Purkinje cells by measuring the inhibitio...
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| Veröffentlicht in: | Neuron (Cambridge, Mass.) Mass.), 2001-10, Vol.32 (2), p.301-313 |
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| creator | Wadiche, Jacques I. Jahr, Craig E. |
| description | Synapses driven by action potentials are thought to release transmitter in an all-or-none fashion; either one synaptic vesicle undergoes exocytosis, or there is no release. We have estimated the glutamate concentration transient at climbing fiber synapses on Purkinje cells by measuring the inhibition of excitatory postsynaptic currents (EPSCs) produced by a low-affinity competitive antagonist of AMPA receptors, γ-DGG. The results, together with simulations using a kinetic model of the AMPA receptor, suggest that the peak glutamate concentration at this synapse is dependent on release probability but is not affected by pooling of transmitter released from neighboring synapses. We propose that the mechanism responsible for the elevated glutamate concentration at this synapse is the simultaneous release of multiple vesicles per site. |
| doi_str_mv | 10.1016/S0896-6273(01)00488-3 |
| format | Article |
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We propose that the mechanism responsible for the elevated glutamate concentration at this synapse is the simultaneous release of multiple vesicles per site.</description><subject>Animals</subject><subject>Biological Transport - drug effects</subject><subject>Calcium - metabolism</subject><subject>climbing fibers</subject><subject>Dipeptides - pharmacology</subject><subject>Excitatory Postsynaptic Potentials - physiology</subject><subject>Glutamic Acid - analysis</subject><subject>Glutamic Acid - metabolism</subject><subject>Kinetics</subject><subject>Kynurenic Acid - pharmacology</subject><subject>Neurotransmitter Agents - metabolism</subject><subject>Purkinje Cells - physiology</subject><subject>Quinoxalines - pharmacology</subject><subject>Rats</subject><subject>Receptors, AMPA - antagonists & inhibitors</subject><subject>Receptors, AMPA - physiology</subject><subject>Synapses - chemistry</subject><subject>Synapses - physiology</subject><subject>Synaptic Transmission</subject><issn>0896-6273</issn><issn>1097-4199</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtOwzAQRS0EoqXwCaCsECwCnsSx4xVCEQWkIhCFtWW7E-SSpsVOKvXvSR-CZVezOffe0SHkHOgNUOC3Y5pLHvNEpFcUrilleR6nB6QPVIqYgZSHpP-H9MhJCFNKgWUSjkkPgOeplLJP7l7aqnFLDM62lfbRO1aoA0a6iYrKzYyrv6KhM-jjt9Z_u3qKUYFVFY1XtV4EDKfkqNRVwLPdHZDP4cNH8RSPXh-fi_tRbJmEJmYlSASWgrFGMJNRSHJgmOci4VxAmSVCZqANlkYkGstJxqwWkCJHKQ3l6YBcbnsXfv7TYmjUzAXbfaJrnLdBiSRhNGOwF4S8c0N51oHZFrR-HoLHUi28m2m_UkDVWrHaKFZrf4qC2ihWaZe72A20ZoaT_9TOaQfcbQHsfCwdehWsw9rixHm0jZrM3Z6JX9F9ihI</recordid><startdate>20011025</startdate><enddate>20011025</enddate><creator>Wadiche, Jacques I.</creator><creator>Jahr, Craig E.</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>20011025</creationdate><title>Multivesicular Release at Climbing Fiber-Purkinje Cell Synapses</title><author>Wadiche, Jacques I. ; Jahr, Craig E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c491t-4f19e1431bcb74b5012814e88726671f527951abefb72aefd54ca713e6e99b063</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Animals</topic><topic>Biological Transport - drug effects</topic><topic>Calcium - metabolism</topic><topic>climbing fibers</topic><topic>Dipeptides - pharmacology</topic><topic>Excitatory Postsynaptic Potentials - physiology</topic><topic>Glutamic Acid - analysis</topic><topic>Glutamic Acid - metabolism</topic><topic>Kinetics</topic><topic>Kynurenic Acid - pharmacology</topic><topic>Neurotransmitter Agents - metabolism</topic><topic>Purkinje Cells - physiology</topic><topic>Quinoxalines - pharmacology</topic><topic>Rats</topic><topic>Receptors, AMPA - antagonists & inhibitors</topic><topic>Receptors, AMPA - physiology</topic><topic>Synapses - chemistry</topic><topic>Synapses - physiology</topic><topic>Synaptic Transmission</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wadiche, Jacques I.</creatorcontrib><creatorcontrib>Jahr, Craig E.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Neuron (Cambridge, Mass.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wadiche, Jacques I.</au><au>Jahr, Craig E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Multivesicular Release at Climbing Fiber-Purkinje Cell Synapses</atitle><jtitle>Neuron (Cambridge, Mass.)</jtitle><addtitle>Neuron</addtitle><date>2001-10-25</date><risdate>2001</risdate><volume>32</volume><issue>2</issue><spage>301</spage><epage>313</epage><pages>301-313</pages><issn>0896-6273</issn><eissn>1097-4199</eissn><abstract>Synapses driven by action potentials are thought to release transmitter in an all-or-none fashion; either one synaptic vesicle undergoes exocytosis, or there is no release. 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| source | MEDLINE; Elsevier ScienceDirect Journals Complete; Cell Press Free Archives; EZB Electronic Journals Library |
| subjects | Animals Biological Transport - drug effects Calcium - metabolism climbing fibers Dipeptides - pharmacology Excitatory Postsynaptic Potentials - physiology Glutamic Acid - analysis Glutamic Acid - metabolism Kinetics Kynurenic Acid - pharmacology Neurotransmitter Agents - metabolism Purkinje Cells - physiology Quinoxalines - pharmacology Rats Receptors, AMPA - antagonists & inhibitors Receptors, AMPA - physiology Synapses - chemistry Synapses - physiology Synaptic Transmission |
| title | Multivesicular Release at Climbing Fiber-Purkinje Cell Synapses |
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