Protein losses in ion-exchange and hydrophobic interaction high-performance liquid chromatography

Protein losses in ion-exchange and hydrophobic interaction HPLC were examined. The supports were all non-porous, packed in columns of identical dimensions. Two ion-exchange chromatography (IEC), anion and cation, as well as a hydrophobic interaction chromatography (HIC) columns were tested. Proteins...

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Veröffentlicht in:	Journal of Chromatography A 2000-08, Vol.890 (1), p.73-80
Hauptverfasser:	Goheen, Steven C, Gibbins, B.M
Format:	Artikel
Sprache:	eng
Schlagworte:	ALBUMINS Analytical, structural and metabolic biochemistry ANIONS BASIC BIOLOGICAL SCIENCES Biological and medical sciences CATTLE CHROMATOGRAPHY Chromatography, High Pressure Liquid - methods Cytochrome c Group - analysis CYTOCHROMES DIMENSIONS FIBRINOGEN Fundamental and applied biological sciences. Psychology General aspects, investigation methods HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY Hydrophobic interaction chromatography Immunoglobulin G - analysis IMMUNOGLOBULINS ION EXCHANGE Ion Exchange Resins - chemistry PROTEINS RETENTION Serum Albumin, Bovine - analysis Temperature TEMPERATURE DEPENDENCE Time Factors
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