Protein delivery by Pseudomonas type III secretion system: Ex vivo complementation of p67(phox)-deficient chronic granulomatous disease
Bacterial type III secretion system drives the translocation of virulence factors into the cystosol of host target cells. In phagocytes and in Epstein-Barr virus immortalized B lymphocytes, NADPH oxidase generates O(-2) through an electron transfer chain the activity of which depends on the assembly...
Gespeichert in:
| Veröffentlicht in: | Biochemical and biophysical research communications 2000-09, Vol.275 (3), p.854-858 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 858 |
|---|---|
| container_issue | 3 |
| container_start_page | 854 |
| container_title | Biochemical and biophysical research communications |
| container_volume | 275 |
| creator | Polack, B Vergnaud, S Paclet, M H Lamotte, D Toussaint, B Morel, F |
| description | Bacterial type III secretion system drives the translocation of virulence factors into the cystosol of host target cells. In phagocytes and in Epstein-Barr virus immortalized B lymphocytes, NADPH oxidase generates O(-2) through an electron transfer chain the activity of which depends on the assembly of three, p67(phox), p47(phox) and p40(phox) cytosolic activating factors with Rac 1/2 and a membrane redox component, cytochrome b(558). In p67(phox) deficient chronic granulomatous disease (CGD) patients, p67-phox is missing and NADPH oxidase activity is abolished. ExoS is a virulence factor of Pseudomonas aeruginosa which is secreted via the type III secretion system: it was fused with p67(phox). Pseudomonas aeruginosa synthesized and translocated the hybrid ExoS-p67(phox) fusion protein into the cytosol of B lymphocytes via the type III secretion system. Purified ExoS-p67(phox) hybrid protein was as efficient as normal recombinant p67(phox) in cell-free reconstitution of NADPH oxidase activity. Therefore, ExoS-p67(phox) was transferred via the type III secretion system of Pseudomonas aeruginosa into the cytosol of B lymphocytes from a p67(phox)-deficient CGD patient and functionally reconstituted NADPH oxidase activity. In the complementation process, ExoS acted as a molecular courier for protein delivery: the reconstitution of an active NADPH oxidase complex suggests type III secretion system to be a new approach for cellular therapy. |
| doi_str_mv | 10.1006/bbrc.2000.3399 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_72239657</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>17621050</sourcerecordid><originalsourceid>FETCH-LOGICAL-p238t-ec2be9292e2d6d56626dfb82d4f3d52ca1374c9c2a37b0be6a0046db86c57ac73</originalsourceid><addsrcrecordid>eNqFkD1PwzAYhD2AaCmsjMgTgiHFH4lTs6GqQKRKdACJrfLHG2qUxMFOquYX8LepoMxMd9I9Op0OoQtKppQQcat1MFNGCJlyLuURGu-tSJikbyN0GuMHIZSmQp6gESUy5zNKx-hrFXwHrsEWKreFMGA94FWE3vraNyribmgBF0WBI5gAnfMNjkPsoL7Dix3euq3HxtdtBTU0nfrJfYlbkV-3G7-7SSyUzrh9hs0m-MYZ_B5U01e-Vp3vI7Yugopwho5LVUU4P-gEvT4sXuZPyfL5sZjfL5OW8VmXgGEaJJMMmBU2E4IJW-oZs2nJbcaMojxPjTRM8VwTDUIRkgqrZ8JkuTI5n6Cr3942-M8eYreuXTRQVaqB_Zx1zhiXIvsfpLlglGRkD14ewF7XYNdtcLUKw_rvY_4NwHF_bg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17621050</pqid></control><display><type>article</type><title>Protein delivery by Pseudomonas type III secretion system: Ex vivo complementation of p67(phox)-deficient chronic granulomatous disease</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><creator>Polack, B ; Vergnaud, S ; Paclet, M H ; Lamotte, D ; Toussaint, B ; Morel, F</creator><creatorcontrib>Polack, B ; Vergnaud, S ; Paclet, M H ; Lamotte, D ; Toussaint, B ; Morel, F</creatorcontrib><description>Bacterial type III secretion system drives the translocation of virulence factors into the cystosol of host target cells. In phagocytes and in Epstein-Barr virus immortalized B lymphocytes, NADPH oxidase generates O(-2) through an electron transfer chain the activity of which depends on the assembly of three, p67(phox), p47(phox) and p40(phox) cytosolic activating factors with Rac 1/2 and a membrane redox component, cytochrome b(558). In p67(phox) deficient chronic granulomatous disease (CGD) patients, p67-phox is missing and NADPH oxidase activity is abolished. ExoS is a virulence factor of Pseudomonas aeruginosa which is secreted via the type III secretion system: it was fused with p67(phox). Pseudomonas aeruginosa synthesized and translocated the hybrid ExoS-p67(phox) fusion protein into the cytosol of B lymphocytes via the type III secretion system. Purified ExoS-p67(phox) hybrid protein was as efficient as normal recombinant p67(phox) in cell-free reconstitution of NADPH oxidase activity. Therefore, ExoS-p67(phox) was transferred via the type III secretion system of Pseudomonas aeruginosa into the cytosol of B lymphocytes from a p67(phox)-deficient CGD patient and functionally reconstituted NADPH oxidase activity. In the complementation process, ExoS acted as a molecular courier for protein delivery: the reconstitution of an active NADPH oxidase complex suggests type III secretion system to be a new approach for cellular therapy.</description><identifier>ISSN: 0006-291X</identifier><identifier>DOI: 10.1006/bbrc.2000.3399</identifier><identifier>PMID: 10973811</identifier><language>eng</language><publisher>United States</publisher><subject>B-Lymphocytes - enzymology ; B-Lymphocytes - metabolism ; B-Lymphocytes - microbiology ; B-Lymphocytes - pathology ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Biological Transport ; Cell Line, Transformed ; chronic granulomatous disease ; Cytosol - enzymology ; Cytosol - metabolism ; ExoS protein ; Genetic Complementation Test ; Granulomatous Disease, Chronic - enzymology ; Granulomatous Disease, Chronic - genetics ; Granulomatous Disease, Chronic - metabolism ; Herpesvirus 4, Human - genetics ; Herpesvirus 4, Human - physiology ; Histidine Kinase ; Humans ; NADPH oxidase ; NADPH Oxidases - metabolism ; Phosphoproteins - deficiency ; Phosphoproteins - genetics ; Phosphoproteins - metabolism ; protein delivery ; Protein Kinases - genetics ; Protein Kinases - metabolism ; Pseudomonas aeruginosa ; Pseudomonas aeruginosa - enzymology ; Pseudomonas aeruginosa - genetics ; Pseudomonas aeruginosa - metabolism ; Recombinant Fusion Proteins - metabolism ; Virulence - genetics</subject><ispartof>Biochemical and biophysical research communications, 2000-09, Vol.275 (3), p.854-858</ispartof><rights>Copyright 2000 Academic Press.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10973811$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Polack, B</creatorcontrib><creatorcontrib>Vergnaud, S</creatorcontrib><creatorcontrib>Paclet, M H</creatorcontrib><creatorcontrib>Lamotte, D</creatorcontrib><creatorcontrib>Toussaint, B</creatorcontrib><creatorcontrib>Morel, F</creatorcontrib><title>Protein delivery by Pseudomonas type III secretion system: Ex vivo complementation of p67(phox)-deficient chronic granulomatous disease</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Bacterial type III secretion system drives the translocation of virulence factors into the cystosol of host target cells. In phagocytes and in Epstein-Barr virus immortalized B lymphocytes, NADPH oxidase generates O(-2) through an electron transfer chain the activity of which depends on the assembly of three, p67(phox), p47(phox) and p40(phox) cytosolic activating factors with Rac 1/2 and a membrane redox component, cytochrome b(558). In p67(phox) deficient chronic granulomatous disease (CGD) patients, p67-phox is missing and NADPH oxidase activity is abolished. ExoS is a virulence factor of Pseudomonas aeruginosa which is secreted via the type III secretion system: it was fused with p67(phox). Pseudomonas aeruginosa synthesized and translocated the hybrid ExoS-p67(phox) fusion protein into the cytosol of B lymphocytes via the type III secretion system. Purified ExoS-p67(phox) hybrid protein was as efficient as normal recombinant p67(phox) in cell-free reconstitution of NADPH oxidase activity. Therefore, ExoS-p67(phox) was transferred via the type III secretion system of Pseudomonas aeruginosa into the cytosol of B lymphocytes from a p67(phox)-deficient CGD patient and functionally reconstituted NADPH oxidase activity. In the complementation process, ExoS acted as a molecular courier for protein delivery: the reconstitution of an active NADPH oxidase complex suggests type III secretion system to be a new approach for cellular therapy.</description><subject>B-Lymphocytes - enzymology</subject><subject>B-Lymphocytes - metabolism</subject><subject>B-Lymphocytes - microbiology</subject><subject>B-Lymphocytes - pathology</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Biological Transport</subject><subject>Cell Line, Transformed</subject><subject>chronic granulomatous disease</subject><subject>Cytosol - enzymology</subject><subject>Cytosol - metabolism</subject><subject>ExoS protein</subject><subject>Genetic Complementation Test</subject><subject>Granulomatous Disease, Chronic - enzymology</subject><subject>Granulomatous Disease, Chronic - genetics</subject><subject>Granulomatous Disease, Chronic - metabolism</subject><subject>Herpesvirus 4, Human - genetics</subject><subject>Herpesvirus 4, Human - physiology</subject><subject>Histidine Kinase</subject><subject>Humans</subject><subject>NADPH oxidase</subject><subject>NADPH Oxidases - metabolism</subject><subject>Phosphoproteins - deficiency</subject><subject>Phosphoproteins - genetics</subject><subject>Phosphoproteins - metabolism</subject><subject>protein delivery</subject><subject>Protein Kinases - genetics</subject><subject>Protein Kinases - metabolism</subject><subject>Pseudomonas aeruginosa</subject><subject>Pseudomonas aeruginosa - enzymology</subject><subject>Pseudomonas aeruginosa - genetics</subject><subject>Pseudomonas aeruginosa - metabolism</subject><subject>Recombinant Fusion Proteins - metabolism</subject><subject>Virulence - genetics</subject><issn>0006-291X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkD1PwzAYhD2AaCmsjMgTgiHFH4lTs6GqQKRKdACJrfLHG2qUxMFOquYX8LepoMxMd9I9Op0OoQtKppQQcat1MFNGCJlyLuURGu-tSJikbyN0GuMHIZSmQp6gESUy5zNKx-hrFXwHrsEWKreFMGA94FWE3vraNyribmgBF0WBI5gAnfMNjkPsoL7Dix3euq3HxtdtBTU0nfrJfYlbkV-3G7-7SSyUzrh9hs0m-MYZ_B5U01e-Vp3vI7Yugopwho5LVUU4P-gEvT4sXuZPyfL5sZjfL5OW8VmXgGEaJJMMmBU2E4IJW-oZs2nJbcaMojxPjTRM8VwTDUIRkgqrZ8JkuTI5n6Cr3942-M8eYreuXTRQVaqB_Zx1zhiXIvsfpLlglGRkD14ewF7XYNdtcLUKw_rvY_4NwHF_bg</recordid><startdate>20000907</startdate><enddate>20000907</enddate><creator>Polack, B</creator><creator>Vergnaud, S</creator><creator>Paclet, M H</creator><creator>Lamotte, D</creator><creator>Toussaint, B</creator><creator>Morel, F</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>20000907</creationdate><title>Protein delivery by Pseudomonas type III secretion system: Ex vivo complementation of p67(phox)-deficient chronic granulomatous disease</title><author>Polack, B ; Vergnaud, S ; Paclet, M H ; Lamotte, D ; Toussaint, B ; Morel, F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p238t-ec2be9292e2d6d56626dfb82d4f3d52ca1374c9c2a37b0be6a0046db86c57ac73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>B-Lymphocytes - enzymology</topic><topic>B-Lymphocytes - metabolism</topic><topic>B-Lymphocytes - microbiology</topic><topic>B-Lymphocytes - pathology</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Biological Transport</topic><topic>Cell Line, Transformed</topic><topic>chronic granulomatous disease</topic><topic>Cytosol - enzymology</topic><topic>Cytosol - metabolism</topic><topic>ExoS protein</topic><topic>Genetic Complementation Test</topic><topic>Granulomatous Disease, Chronic - enzymology</topic><topic>Granulomatous Disease, Chronic - genetics</topic><topic>Granulomatous Disease, Chronic - metabolism</topic><topic>Herpesvirus 4, Human - genetics</topic><topic>Herpesvirus 4, Human - physiology</topic><topic>Histidine Kinase</topic><topic>Humans</topic><topic>NADPH oxidase</topic><topic>NADPH Oxidases - metabolism</topic><topic>Phosphoproteins - deficiency</topic><topic>Phosphoproteins - genetics</topic><topic>Phosphoproteins - metabolism</topic><topic>protein delivery</topic><topic>Protein Kinases - genetics</topic><topic>Protein Kinases - metabolism</topic><topic>Pseudomonas aeruginosa</topic><topic>Pseudomonas aeruginosa - enzymology</topic><topic>Pseudomonas aeruginosa - genetics</topic><topic>Pseudomonas aeruginosa - metabolism</topic><topic>Recombinant Fusion Proteins - metabolism</topic><topic>Virulence - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Polack, B</creatorcontrib><creatorcontrib>Vergnaud, S</creatorcontrib><creatorcontrib>Paclet, M H</creatorcontrib><creatorcontrib>Lamotte, D</creatorcontrib><creatorcontrib>Toussaint, B</creatorcontrib><creatorcontrib>Morel, F</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Polack, B</au><au>Vergnaud, S</au><au>Paclet, M H</au><au>Lamotte, D</au><au>Toussaint, B</au><au>Morel, F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Protein delivery by Pseudomonas type III secretion system: Ex vivo complementation of p67(phox)-deficient chronic granulomatous disease</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2000-09-07</date><risdate>2000</risdate><volume>275</volume><issue>3</issue><spage>854</spage><epage>858</epage><pages>854-858</pages><issn>0006-291X</issn><abstract>Bacterial type III secretion system drives the translocation of virulence factors into the cystosol of host target cells. In phagocytes and in Epstein-Barr virus immortalized B lymphocytes, NADPH oxidase generates O(-2) through an electron transfer chain the activity of which depends on the assembly of three, p67(phox), p47(phox) and p40(phox) cytosolic activating factors with Rac 1/2 and a membrane redox component, cytochrome b(558). In p67(phox) deficient chronic granulomatous disease (CGD) patients, p67-phox is missing and NADPH oxidase activity is abolished. ExoS is a virulence factor of Pseudomonas aeruginosa which is secreted via the type III secretion system: it was fused with p67(phox). Pseudomonas aeruginosa synthesized and translocated the hybrid ExoS-p67(phox) fusion protein into the cytosol of B lymphocytes via the type III secretion system. Purified ExoS-p67(phox) hybrid protein was as efficient as normal recombinant p67(phox) in cell-free reconstitution of NADPH oxidase activity. Therefore, ExoS-p67(phox) was transferred via the type III secretion system of Pseudomonas aeruginosa into the cytosol of B lymphocytes from a p67(phox)-deficient CGD patient and functionally reconstituted NADPH oxidase activity. In the complementation process, ExoS acted as a molecular courier for protein delivery: the reconstitution of an active NADPH oxidase complex suggests type III secretion system to be a new approach for cellular therapy.</abstract><cop>United States</cop><pmid>10973811</pmid><doi>10.1006/bbrc.2000.3399</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-291X |
| ispartof | Biochemical and biophysical research communications, 2000-09, Vol.275 (3), p.854-858 |
| issn | 0006-291X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_72239657 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | B-Lymphocytes - enzymology B-Lymphocytes - metabolism B-Lymphocytes - microbiology B-Lymphocytes - pathology Bacterial Proteins - genetics Bacterial Proteins - metabolism Biological Transport Cell Line, Transformed chronic granulomatous disease Cytosol - enzymology Cytosol - metabolism ExoS protein Genetic Complementation Test Granulomatous Disease, Chronic - enzymology Granulomatous Disease, Chronic - genetics Granulomatous Disease, Chronic - metabolism Herpesvirus 4, Human - genetics Herpesvirus 4, Human - physiology Histidine Kinase Humans NADPH oxidase NADPH Oxidases - metabolism Phosphoproteins - deficiency Phosphoproteins - genetics Phosphoproteins - metabolism protein delivery Protein Kinases - genetics Protein Kinases - metabolism Pseudomonas aeruginosa Pseudomonas aeruginosa - enzymology Pseudomonas aeruginosa - genetics Pseudomonas aeruginosa - metabolism Recombinant Fusion Proteins - metabolism Virulence - genetics |
| title | Protein delivery by Pseudomonas type III secretion system: Ex vivo complementation of p67(phox)-deficient chronic granulomatous disease |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-13T03%3A27%3A35IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Protein%20delivery%20by%20Pseudomonas%20type%20III%20secretion%20system:%20Ex%20vivo%20complementation%20of%20p67(phox)-deficient%20chronic%20granulomatous%20disease&rft.jtitle=Biochemical%20and%20biophysical%20research%20communications&rft.au=Polack,%20B&rft.date=2000-09-07&rft.volume=275&rft.issue=3&rft.spage=854&rft.epage=858&rft.pages=854-858&rft.issn=0006-291X&rft_id=info:doi/10.1006/bbrc.2000.3399&rft_dat=%3Cproquest_pubme%3E17621050%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=17621050&rft_id=info:pmid/10973811&rfr_iscdi=true |