Development of oligonucleotide lateral-flow immunoassay for multi-parameter detection
We have developed a highly sensitive and rapid oligonucleotide lateral-flow immunoassay (OLFIA), using a colloidal gold as an indicator, for the simultaneous detection of antigens and/or antibodies in specimen. This system can detect more than two types of antigens and/or antibodies in a single assa...
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| Veröffentlicht in: | Journal of immunological methods 2001-12, Vol.258 (1), p.73-84 |
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| container_title | Journal of immunological methods |
| container_volume | 258 |
| creator | Oku, Yuichi Kamiya, Kumiko Kamiya, Hisanori Shibahara, Yusuke Ii, Toshihiro Uesaka, Yoshihiko |
| description | We have developed a highly sensitive and rapid oligonucleotide lateral-flow immunoassay (OLFIA), using a colloidal gold as an indicator, for the simultaneous detection of antigens and/or antibodies in specimen. This system can detect more than two types of antigens and/or antibodies in a single assay device at the same time. The device is basically composed of colloidal gold-labeled antibodies and oligonucleotide-labeled antibodies fixed in a conjugate pad, and the complementary oligonucleotide-labeled proteins are immobilized on a nitrocellulose membrane. If the target antigen is present in a specimen, the colloidal gold-labeled antibody and oligonucleotide-labeled antibody will make a complex with the antigen. Subsequently, the formed complex migrates to the place where complementary oligonucleotide is immobilized and is bound to the solid phase via the DNA–DNA interaction. As a result, more than two types of reactions can be detected on a single assay device by the combination of colloidal gold-labeled antibodies, different oligonucleotide-labeled antibodies and complementary oligonucleotide-labeled proteins immobilized at different places on a nitrocellulose membrane. |
| doi_str_mv | 10.1016/S0022-1759(01)00470-7 |
| format | Article |
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This system can detect more than two types of antigens and/or antibodies in a single assay device at the same time. The device is basically composed of colloidal gold-labeled antibodies and oligonucleotide-labeled antibodies fixed in a conjugate pad, and the complementary oligonucleotide-labeled proteins are immobilized on a nitrocellulose membrane. If the target antigen is present in a specimen, the colloidal gold-labeled antibody and oligonucleotide-labeled antibody will make a complex with the antigen. Subsequently, the formed complex migrates to the place where complementary oligonucleotide is immobilized and is bound to the solid phase via the DNA–DNA interaction. As a result, more than two types of reactions can be detected on a single assay device by the combination of colloidal gold-labeled antibodies, different oligonucleotide-labeled antibodies and complementary oligonucleotide-labeled proteins immobilized at different places on a nitrocellulose membrane.</description><identifier>ISSN: 0022-1759</identifier><identifier>EISSN: 1872-7905</identifier><identifier>DOI: 10.1016/S0022-1759(01)00470-7</identifier><identifier>PMID: 11684125</identifier><identifier>CODEN: JIMMBG</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Antibodies - analysis ; Antibodies, Monoclonal ; Antigen-Antibody Reactions ; Antigens - analysis ; Bacteriological methods and techniques used in bacteriology ; Bacteriology ; Base Sequence ; Biological and medical sciences ; Clinical Immunology ; Colloidal gold ; Diverse techniques ; Fundamental and applied biological sciences. Psychology ; gold ; Gold Colloid ; Humans ; Immunoassay ; Immunoassay - methods ; Immunoassay - statistics & numerical data ; Immunochromatography ; Journal category ; Mice ; Microbiology ; Molecular and cellular biology ; Nitrocellulose ; Oligodeoxyribonucleotides - genetics ; Oligonucleotide ; oligonucleotides ; Sensitivity and Specificity</subject><ispartof>Journal of immunological methods, 2001-12, Vol.258 (1), p.73-84</ispartof><rights>2001 Elsevier Science B.V.</rights><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c422t-bcccafcd9f6ea3b629669fa6d1945bf994442a1980c0f7633b791fbd3f2ab2843</citedby><cites>FETCH-LOGICAL-c422t-bcccafcd9f6ea3b629669fa6d1945bf994442a1980c0f7633b791fbd3f2ab2843</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0022175901004707$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14160828$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11684125$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Oku, Yuichi</creatorcontrib><creatorcontrib>Kamiya, Kumiko</creatorcontrib><creatorcontrib>Kamiya, Hisanori</creatorcontrib><creatorcontrib>Shibahara, Yusuke</creatorcontrib><creatorcontrib>Ii, Toshihiro</creatorcontrib><creatorcontrib>Uesaka, Yoshihiko</creatorcontrib><title>Development of oligonucleotide lateral-flow immunoassay for multi-parameter detection</title><title>Journal of immunological methods</title><addtitle>J Immunol Methods</addtitle><description>We have developed a highly sensitive and rapid oligonucleotide lateral-flow immunoassay (OLFIA), using a colloidal gold as an indicator, for the simultaneous detection of antigens and/or antibodies in specimen. This system can detect more than two types of antigens and/or antibodies in a single assay device at the same time. The device is basically composed of colloidal gold-labeled antibodies and oligonucleotide-labeled antibodies fixed in a conjugate pad, and the complementary oligonucleotide-labeled proteins are immobilized on a nitrocellulose membrane. If the target antigen is present in a specimen, the colloidal gold-labeled antibody and oligonucleotide-labeled antibody will make a complex with the antigen. Subsequently, the formed complex migrates to the place where complementary oligonucleotide is immobilized and is bound to the solid phase via the DNA–DNA interaction. As a result, more than two types of reactions can be detected on a single assay device by the combination of colloidal gold-labeled antibodies, different oligonucleotide-labeled antibodies and complementary oligonucleotide-labeled proteins immobilized at different places on a nitrocellulose membrane.</description><subject>Animals</subject><subject>Antibodies - analysis</subject><subject>Antibodies, Monoclonal</subject><subject>Antigen-Antibody Reactions</subject><subject>Antigens - analysis</subject><subject>Bacteriological methods and techniques used in bacteriology</subject><subject>Bacteriology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Clinical Immunology</subject><subject>Colloidal gold</subject><subject>Diverse techniques</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gold</subject><subject>Gold Colloid</subject><subject>Humans</subject><subject>Immunoassay</subject><subject>Immunoassay - methods</subject><subject>Immunoassay - statistics & numerical data</subject><subject>Immunochromatography</subject><subject>Journal category</subject><subject>Mice</subject><subject>Microbiology</subject><subject>Molecular and cellular biology</subject><subject>Nitrocellulose</subject><subject>Oligodeoxyribonucleotides - genetics</subject><subject>Oligonucleotide</subject><subject>oligonucleotides</subject><subject>Sensitivity and Specificity</subject><issn>0022-1759</issn><issn>1872-7905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0MFu1DAQgGELUdGl5RFAuVDRQ-iM49jxCaFCKVIlDtCz5ThjZOTEi50U9e2b7a7osRf78tkz-hl7i_ARAeXFTwDOa1St_gB4DiAU1OoF22CneK00tC_Z5j85Zq9L-QMACBJesWNE2Qnk7YbdfqE7imk70jRXyVcpht9pWlykNIeBqmhnyjbWPqZ_VRjHZUq2FHtf-ZSrcYlzqLc225FWVg3r6eaQplN25G0s9OZwn7Dbq6-_Lq_rmx_fvl9-vqmd4Hyue-ec9W7QXpJtesm1lNpbOaAWbe-1FkJwi7oDB17JpumVRt8Pjee2551oTtjZ_t9tTn8XKrMZQ3EUo50oLcUozhvFtXoWYoccdNutsN1Dl1MpmbzZ5jDafG8QzC68eQxvdlUNoHkMb3YD3h0GLP1Iw9OrQ-kVvD8AW5yNPtvJhfLkBEro-G6BT3tHa7e7QNkUF2hyNIS8xjVDCs-s8gCLw6Dy</recordid><startdate>20011201</startdate><enddate>20011201</enddate><creator>Oku, Yuichi</creator><creator>Kamiya, Kumiko</creator><creator>Kamiya, Hisanori</creator><creator>Shibahara, Yusuke</creator><creator>Ii, Toshihiro</creator><creator>Uesaka, Yoshihiko</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T5</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20011201</creationdate><title>Development of oligonucleotide lateral-flow immunoassay for multi-parameter detection</title><author>Oku, Yuichi ; Kamiya, Kumiko ; Kamiya, Hisanori ; Shibahara, Yusuke ; Ii, Toshihiro ; Uesaka, Yoshihiko</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-bcccafcd9f6ea3b629669fa6d1945bf994442a1980c0f7633b791fbd3f2ab2843</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Animals</topic><topic>Antibodies - analysis</topic><topic>Antibodies, Monoclonal</topic><topic>Antigen-Antibody Reactions</topic><topic>Antigens - analysis</topic><topic>Bacteriological methods and techniques used in bacteriology</topic><topic>Bacteriology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Clinical Immunology</topic><topic>Colloidal gold</topic><topic>Diverse techniques</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gold</topic><topic>Gold Colloid</topic><topic>Humans</topic><topic>Immunoassay</topic><topic>Immunoassay - methods</topic><topic>Immunoassay - statistics & numerical data</topic><topic>Immunochromatography</topic><topic>Journal category</topic><topic>Mice</topic><topic>Microbiology</topic><topic>Molecular and cellular biology</topic><topic>Nitrocellulose</topic><topic>Oligodeoxyribonucleotides - genetics</topic><topic>Oligonucleotide</topic><topic>oligonucleotides</topic><topic>Sensitivity and Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Oku, Yuichi</creatorcontrib><creatorcontrib>Kamiya, Kumiko</creatorcontrib><creatorcontrib>Kamiya, Hisanori</creatorcontrib><creatorcontrib>Shibahara, Yusuke</creatorcontrib><creatorcontrib>Ii, Toshihiro</creatorcontrib><creatorcontrib>Uesaka, Yoshihiko</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of immunological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Oku, Yuichi</au><au>Kamiya, Kumiko</au><au>Kamiya, Hisanori</au><au>Shibahara, Yusuke</au><au>Ii, Toshihiro</au><au>Uesaka, Yoshihiko</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of oligonucleotide lateral-flow immunoassay for multi-parameter detection</atitle><jtitle>Journal of immunological methods</jtitle><addtitle>J Immunol Methods</addtitle><date>2001-12-01</date><risdate>2001</risdate><volume>258</volume><issue>1</issue><spage>73</spage><epage>84</epage><pages>73-84</pages><issn>0022-1759</issn><eissn>1872-7905</eissn><coden>JIMMBG</coden><abstract>We have developed a highly sensitive and rapid oligonucleotide lateral-flow immunoassay (OLFIA), using a colloidal gold as an indicator, for the simultaneous detection of antigens and/or antibodies in specimen. This system can detect more than two types of antigens and/or antibodies in a single assay device at the same time. The device is basically composed of colloidal gold-labeled antibodies and oligonucleotide-labeled antibodies fixed in a conjugate pad, and the complementary oligonucleotide-labeled proteins are immobilized on a nitrocellulose membrane. If the target antigen is present in a specimen, the colloidal gold-labeled antibody and oligonucleotide-labeled antibody will make a complex with the antigen. Subsequently, the formed complex migrates to the place where complementary oligonucleotide is immobilized and is bound to the solid phase via the DNA–DNA interaction. 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| ispartof | Journal of immunological methods, 2001-12, Vol.258 (1), p.73-84 |
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| subjects | Animals Antibodies - analysis Antibodies, Monoclonal Antigen-Antibody Reactions Antigens - analysis Bacteriological methods and techniques used in bacteriology Bacteriology Base Sequence Biological and medical sciences Clinical Immunology Colloidal gold Diverse techniques Fundamental and applied biological sciences. Psychology gold Gold Colloid Humans Immunoassay Immunoassay - methods Immunoassay - statistics & numerical data Immunochromatography Journal category Mice Microbiology Molecular and cellular biology Nitrocellulose Oligodeoxyribonucleotides - genetics Oligonucleotide oligonucleotides Sensitivity and Specificity |
| title | Development of oligonucleotide lateral-flow immunoassay for multi-parameter detection |
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