Mitochondrial DNA heteroplasmy after human ooplasmic transplantation
Objective: To determine the patterns of mitochondrial inheritance in embryos, fetuses, and infants after ooplasmic transplantation using the technique of mitochondrial DNA (mtDNA) fingerprinting. Design: Prospective clinical study. Setting: The IVF program at Saint Barnabas Medical Center, a nonprof...
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| Veröffentlicht in: | Fertility and sterility 2000-09, Vol.74 (3), p.573-578 |
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| creator | Brenner, Carol A Barritt, Jason A Willadsen, Steen Cohen, Jacques |
| description | Objective: To determine the patterns of mitochondrial inheritance in embryos, fetuses, and infants after ooplasmic transplantation using the technique of mitochondrial DNA (mtDNA) fingerprinting.
Design: Prospective clinical study.
Setting: The IVF program at Saint Barnabas Medical Center, a nonprofit community hospital.
Patient(s): In a total of 23 cases with recurrent implantation failure after IVF ooplasmic transplantation was performed. Thirteen embryos from two patients and amniotic cells from four patients were investigated for heteroplasmy. Placenta and fetal cord blood cells from four newborn babies/infants were also investigated.
Intervention(s): None.
Main Outcome Measure(s): mtDNA fingerprinting, polymerase chain reaction, and DNA sequencing analysis.
Result(s): In addition to the recipient maternal mitochondrial DNA, a small proportion of donor mitochondrial DNA was detected in samples with the following frequencies: embryos (n = 6/13), amniocytes (n = 1/4), placenta (n = 2/4), and fetal cord blood (n = 2/4). Fingerprinting showed that nuclear DNA was not inherited from the donor in placenta or fetal cord blood of the babies.
Conclusion(s): Ooplasmic transfer can result in sustained mtDNA heteroplasmy representing both donor and recipient. This was shown by mtDNA fingerprinting of embryos, amniocytes, fetal placenta, and cord blood. These results show that the donor-derived mitochondrial population persists after ooplasmic transfer and may be replicated during fetal development. |
| doi_str_mv | 10.1016/S0015-0282(00)00681-6 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_72236215</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0015028200006816</els_id><sourcerecordid>72236215</sourcerecordid><originalsourceid>FETCH-LOGICAL-c503t-a2cf4f27b3d492c7b6dc2ac4557a7b146a3a3197d6808634245a5c7fc2ee83843</originalsourceid><addsrcrecordid>eNqFkE1PwzAMhiMEgjH4CaAeEIJDwUmapDuhaeNLGnAAzlGWplpQ24ykRdq_J1sn4MbJsvXYfvUgdILhCgPm168AmKVAcnIBcAnAc5zyHTTAjPGUcUZ30eAHOUCHIXxApLAg--gAw0hQzsQATZ9s6_TCNYW3qkqmz-NkYVrj3bJSoV4lqoxNsuhq1SSuH1qdtF41ITZNq1rrmiO0V6oqmONtHaL3u9u3yUM6e7l_nIxnqWZA21QRXWYlEXNaZCOixZwXmiidMSaUmOOMK6ooHomC55BzmpGMKaZFqYkxOc0zOkTn_d2ld5-dCa2sbdCmikGM64IUhFBOMIsg60HtXQjelHLpba38SmKQa31yo0-u3UgAudEnedw73T7o5rUp_mz1viJwtgVU0Koqowdtwy_HcDy0xm56zEQbX9Z4GbQ1jTaF9Ua3snD2nyTfdSKLuA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>72236215</pqid></control><display><type>article</type><title>Mitochondrial DNA heteroplasmy after human ooplasmic transplantation</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Alma/SFX Local Collection</source><creator>Brenner, Carol A ; Barritt, Jason A ; Willadsen, Steen ; Cohen, Jacques</creator><creatorcontrib>Brenner, Carol A ; Barritt, Jason A ; Willadsen, Steen ; Cohen, Jacques</creatorcontrib><description>Objective: To determine the patterns of mitochondrial inheritance in embryos, fetuses, and infants after ooplasmic transplantation using the technique of mitochondrial DNA (mtDNA) fingerprinting.
Design: Prospective clinical study.
Setting: The IVF program at Saint Barnabas Medical Center, a nonprofit community hospital.
Patient(s): In a total of 23 cases with recurrent implantation failure after IVF ooplasmic transplantation was performed. Thirteen embryos from two patients and amniotic cells from four patients were investigated for heteroplasmy. Placenta and fetal cord blood cells from four newborn babies/infants were also investigated.
Intervention(s): None.
Main Outcome Measure(s): mtDNA fingerprinting, polymerase chain reaction, and DNA sequencing analysis.
Result(s): In addition to the recipient maternal mitochondrial DNA, a small proportion of donor mitochondrial DNA was detected in samples with the following frequencies: embryos (n = 6/13), amniocytes (n = 1/4), placenta (n = 2/4), and fetal cord blood (n = 2/4). Fingerprinting showed that nuclear DNA was not inherited from the donor in placenta or fetal cord blood of the babies.
Conclusion(s): Ooplasmic transfer can result in sustained mtDNA heteroplasmy representing both donor and recipient. This was shown by mtDNA fingerprinting of embryos, amniocytes, fetal placenta, and cord blood. These results show that the donor-derived mitochondrial population persists after ooplasmic transfer and may be replicated during fetal development.</description><identifier>ISSN: 0015-0282</identifier><identifier>EISSN: 1556-5653</identifier><identifier>DOI: 10.1016/S0015-0282(00)00681-6</identifier><identifier>PMID: 10973657</identifier><identifier>CODEN: FESTAS</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Biological and medical sciences ; Birth control ; Cytoplasm - transplantation ; Cytoplasmic transplantation ; DNA Fingerprinting ; DNA, Mitochondrial - chemistry ; Female ; Fertilization in Vitro ; Fetal Blood - chemistry ; Gynecology. Andrology. Obstetrics ; human embryo ; human oocyte ; Humans ; Infertility, Female - therapy ; Medical sciences ; mitochondrial DNA ; mitochondrial DNA heteroplasmy ; Oocytes ; Polymerase Chain Reaction ; Pregnancy ; Prospective Studies ; Sequence Analysis, DNA ; Sterility. Assisted procreation</subject><ispartof>Fertility and sterility, 2000-09, Vol.74 (3), p.573-578</ispartof><rights>2000 American Society for Reproductive Medicine</rights><rights>2000 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c503t-a2cf4f27b3d492c7b6dc2ac4557a7b146a3a3197d6808634245a5c7fc2ee83843</citedby><cites>FETCH-LOGICAL-c503t-a2cf4f27b3d492c7b6dc2ac4557a7b146a3a3197d6808634245a5c7fc2ee83843</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0015028200006816$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1516817$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10973657$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Brenner, Carol A</creatorcontrib><creatorcontrib>Barritt, Jason A</creatorcontrib><creatorcontrib>Willadsen, Steen</creatorcontrib><creatorcontrib>Cohen, Jacques</creatorcontrib><title>Mitochondrial DNA heteroplasmy after human ooplasmic transplantation</title><title>Fertility and sterility</title><addtitle>Fertil Steril</addtitle><description>Objective: To determine the patterns of mitochondrial inheritance in embryos, fetuses, and infants after ooplasmic transplantation using the technique of mitochondrial DNA (mtDNA) fingerprinting.
Design: Prospective clinical study.
Setting: The IVF program at Saint Barnabas Medical Center, a nonprofit community hospital.
Patient(s): In a total of 23 cases with recurrent implantation failure after IVF ooplasmic transplantation was performed. Thirteen embryos from two patients and amniotic cells from four patients were investigated for heteroplasmy. Placenta and fetal cord blood cells from four newborn babies/infants were also investigated.
Intervention(s): None.
Main Outcome Measure(s): mtDNA fingerprinting, polymerase chain reaction, and DNA sequencing analysis.
Result(s): In addition to the recipient maternal mitochondrial DNA, a small proportion of donor mitochondrial DNA was detected in samples with the following frequencies: embryos (n = 6/13), amniocytes (n = 1/4), placenta (n = 2/4), and fetal cord blood (n = 2/4). Fingerprinting showed that nuclear DNA was not inherited from the donor in placenta or fetal cord blood of the babies.
Conclusion(s): Ooplasmic transfer can result in sustained mtDNA heteroplasmy representing both donor and recipient. This was shown by mtDNA fingerprinting of embryos, amniocytes, fetal placenta, and cord blood. These results show that the donor-derived mitochondrial population persists after ooplasmic transfer and may be replicated during fetal development.</description><subject>Biological and medical sciences</subject><subject>Birth control</subject><subject>Cytoplasm - transplantation</subject><subject>Cytoplasmic transplantation</subject><subject>DNA Fingerprinting</subject><subject>DNA, Mitochondrial - chemistry</subject><subject>Female</subject><subject>Fertilization in Vitro</subject><subject>Fetal Blood - chemistry</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>human embryo</subject><subject>human oocyte</subject><subject>Humans</subject><subject>Infertility, Female - therapy</subject><subject>Medical sciences</subject><subject>mitochondrial DNA</subject><subject>mitochondrial DNA heteroplasmy</subject><subject>Oocytes</subject><subject>Polymerase Chain Reaction</subject><subject>Pregnancy</subject><subject>Prospective Studies</subject><subject>Sequence Analysis, DNA</subject><subject>Sterility. Assisted procreation</subject><issn>0015-0282</issn><issn>1556-5653</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1PwzAMhiMEgjH4CaAeEIJDwUmapDuhaeNLGnAAzlGWplpQ24ykRdq_J1sn4MbJsvXYfvUgdILhCgPm168AmKVAcnIBcAnAc5zyHTTAjPGUcUZ30eAHOUCHIXxApLAg--gAw0hQzsQATZ9s6_TCNYW3qkqmz-NkYVrj3bJSoV4lqoxNsuhq1SSuH1qdtF41ITZNq1rrmiO0V6oqmONtHaL3u9u3yUM6e7l_nIxnqWZA21QRXWYlEXNaZCOixZwXmiidMSaUmOOMK6ooHomC55BzmpGMKaZFqYkxOc0zOkTn_d2ld5-dCa2sbdCmikGM64IUhFBOMIsg60HtXQjelHLpba38SmKQa31yo0-u3UgAudEnedw73T7o5rUp_mz1viJwtgVU0Koqowdtwy_HcDy0xm56zEQbX9Z4GbQ1jTaF9Ua3snD2nyTfdSKLuA</recordid><startdate>20000901</startdate><enddate>20000901</enddate><creator>Brenner, Carol A</creator><creator>Barritt, Jason A</creator><creator>Willadsen, Steen</creator><creator>Cohen, Jacques</creator><general>Elsevier Inc</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20000901</creationdate><title>Mitochondrial DNA heteroplasmy after human ooplasmic transplantation</title><author>Brenner, Carol A ; Barritt, Jason A ; Willadsen, Steen ; Cohen, Jacques</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c503t-a2cf4f27b3d492c7b6dc2ac4557a7b146a3a3197d6808634245a5c7fc2ee83843</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Biological and medical sciences</topic><topic>Birth control</topic><topic>Cytoplasm - transplantation</topic><topic>Cytoplasmic transplantation</topic><topic>DNA Fingerprinting</topic><topic>DNA, Mitochondrial - chemistry</topic><topic>Female</topic><topic>Fertilization in Vitro</topic><topic>Fetal Blood - chemistry</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>human embryo</topic><topic>human oocyte</topic><topic>Humans</topic><topic>Infertility, Female - therapy</topic><topic>Medical sciences</topic><topic>mitochondrial DNA</topic><topic>mitochondrial DNA heteroplasmy</topic><topic>Oocytes</topic><topic>Polymerase Chain Reaction</topic><topic>Pregnancy</topic><topic>Prospective Studies</topic><topic>Sequence Analysis, DNA</topic><topic>Sterility. Assisted procreation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Brenner, Carol A</creatorcontrib><creatorcontrib>Barritt, Jason A</creatorcontrib><creatorcontrib>Willadsen, Steen</creatorcontrib><creatorcontrib>Cohen, Jacques</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Fertility and sterility</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Brenner, Carol A</au><au>Barritt, Jason A</au><au>Willadsen, Steen</au><au>Cohen, Jacques</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mitochondrial DNA heteroplasmy after human ooplasmic transplantation</atitle><jtitle>Fertility and sterility</jtitle><addtitle>Fertil Steril</addtitle><date>2000-09-01</date><risdate>2000</risdate><volume>74</volume><issue>3</issue><spage>573</spage><epage>578</epage><pages>573-578</pages><issn>0015-0282</issn><eissn>1556-5653</eissn><coden>FESTAS</coden><abstract>Objective: To determine the patterns of mitochondrial inheritance in embryos, fetuses, and infants after ooplasmic transplantation using the technique of mitochondrial DNA (mtDNA) fingerprinting.
Design: Prospective clinical study.
Setting: The IVF program at Saint Barnabas Medical Center, a nonprofit community hospital.
Patient(s): In a total of 23 cases with recurrent implantation failure after IVF ooplasmic transplantation was performed. Thirteen embryos from two patients and amniotic cells from four patients were investigated for heteroplasmy. Placenta and fetal cord blood cells from four newborn babies/infants were also investigated.
Intervention(s): None.
Main Outcome Measure(s): mtDNA fingerprinting, polymerase chain reaction, and DNA sequencing analysis.
Result(s): In addition to the recipient maternal mitochondrial DNA, a small proportion of donor mitochondrial DNA was detected in samples with the following frequencies: embryos (n = 6/13), amniocytes (n = 1/4), placenta (n = 2/4), and fetal cord blood (n = 2/4). Fingerprinting showed that nuclear DNA was not inherited from the donor in placenta or fetal cord blood of the babies.
Conclusion(s): Ooplasmic transfer can result in sustained mtDNA heteroplasmy representing both donor and recipient. This was shown by mtDNA fingerprinting of embryos, amniocytes, fetal placenta, and cord blood. These results show that the donor-derived mitochondrial population persists after ooplasmic transfer and may be replicated during fetal development.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>10973657</pmid><doi>10.1016/S0015-0282(00)00681-6</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Biological and medical sciences Birth control Cytoplasm - transplantation Cytoplasmic transplantation DNA Fingerprinting DNA, Mitochondrial - chemistry Female Fertilization in Vitro Fetal Blood - chemistry Gynecology. Andrology. Obstetrics human embryo human oocyte Humans Infertility, Female - therapy Medical sciences mitochondrial DNA mitochondrial DNA heteroplasmy Oocytes Polymerase Chain Reaction Pregnancy Prospective Studies Sequence Analysis, DNA Sterility. Assisted procreation |
| title | Mitochondrial DNA heteroplasmy after human ooplasmic transplantation |
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