A transcript encoding a proteasome beta-subunit and a zinc finger protein in Trypanosoma brucei brucei
During the screening of a Trypanosoma brucei brucei ( T. b. brucei) cDNA library constructed from bloodstream form mRNA, we identified a 2.3 kb cDNA encoding a proteasome beta subunit (ORF1) and a putative zinc finger protein (ORF2). Northern blot analysis indicated the presence of a digenic transcr...
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| Veröffentlicht in: | Gene 2000-09, Vol.255 (1), p.43-50 |
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| creator | Radwanska, Magdalena Couvreur, Bernard Dumont, Nathalie Pays, Annette Vanhamme, Luc Pays, Etienne |
| description | During the screening of a
Trypanosoma brucei brucei (
T. b. brucei) cDNA library constructed from bloodstream form mRNA, we identified a 2.3
kb cDNA encoding a proteasome beta subunit (ORF1) and a putative zinc finger protein (ORF2). Northern blot analysis indicated the presence of a digenic transcript as well as the two individual messengers in both procyclic and bloodstream forms of the parasite. Southern blot analysis showed the relevant locus to be unique. ORF1 encoded a 22.7
kDa protein sharing over 50% identity with the eukaryotic PRCE (aka beta5) proteasome beta subunit. This protein contained a beta amino acid signature and residues involved in the catalytic activity. Further phylogenetic analysis indicated that this subunit as well as those from other kinetoplastids could be confidentially assigned to extant eukaryotic subfamilies such as beta1, beta2, and beta5. ORF2 encoded a 14.6
kDa putative zinc finger protein containing five repeats of a CCHC motif commonly present in retroviral nucleocapsid proteins as well as proteins involved in vertebrate embryogenesis. |
| doi_str_mv | 10.1016/S0378-1119(00)00306-1 |
| format | Article |
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Trypanosoma brucei brucei (
T. b. brucei) cDNA library constructed from bloodstream form mRNA, we identified a 2.3
kb cDNA encoding a proteasome beta subunit (ORF1) and a putative zinc finger protein (ORF2). Northern blot analysis indicated the presence of a digenic transcript as well as the two individual messengers in both procyclic and bloodstream forms of the parasite. Southern blot analysis showed the relevant locus to be unique. ORF1 encoded a 22.7
kDa protein sharing over 50% identity with the eukaryotic PRCE (aka beta5) proteasome beta subunit. This protein contained a beta amino acid signature and residues involved in the catalytic activity. Further phylogenetic analysis indicated that this subunit as well as those from other kinetoplastids could be confidentially assigned to extant eukaryotic subfamilies such as beta1, beta2, and beta5. ORF2 encoded a 14.6
kDa putative zinc finger protein containing five repeats of a CCHC motif commonly present in retroviral nucleocapsid proteins as well as proteins involved in vertebrate embryogenesis.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/S0378-1119(00)00306-1</identifier><identifier>PMID: 10974563</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Amino Acid Sequence ; Animals ; Base Sequence ; Cysteine Endopeptidases - genetics ; DNA, Complementary - chemistry ; DNA, Complementary - genetics ; Molecular Sequence Data ; Multienzyme Complexes - genetics ; Open Reading Frames ; Orf1 gene ; Orf2 gene ; Phylogenetic analysis ; Phylogeny ; Polycistronic transcription ; Proteasome Endopeptidase Complex ; proteasomes ; Protein Subunits ; RNA, Messenger - genetics ; Sequence Alignment ; Sequence Analysis, DNA ; Sequence Homology, Amino Acid ; Transcription, Genetic ; Trypanosoma brucei ; Trypanosoma brucei brucei - genetics ; Zinc Fingers - genetics</subject><ispartof>Gene, 2000-09, Vol.255 (1), p.43-50</ispartof><rights>2000 Elsevier Science B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c392t-4e9ec55dcf9af9bf7a91bb40fe210a7c40321d6137972042fca903b438f85a4b3</citedby><cites>FETCH-LOGICAL-c392t-4e9ec55dcf9af9bf7a91bb40fe210a7c40321d6137972042fca903b438f85a4b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0378-1119(00)00306-1$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10974563$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Radwanska, Magdalena</creatorcontrib><creatorcontrib>Couvreur, Bernard</creatorcontrib><creatorcontrib>Dumont, Nathalie</creatorcontrib><creatorcontrib>Pays, Annette</creatorcontrib><creatorcontrib>Vanhamme, Luc</creatorcontrib><creatorcontrib>Pays, Etienne</creatorcontrib><title>A transcript encoding a proteasome beta-subunit and a zinc finger protein in Trypanosoma brucei brucei</title><title>Gene</title><addtitle>Gene</addtitle><description>During the screening of a
Trypanosoma brucei brucei (
T. b. brucei) cDNA library constructed from bloodstream form mRNA, we identified a 2.3
kb cDNA encoding a proteasome beta subunit (ORF1) and a putative zinc finger protein (ORF2). Northern blot analysis indicated the presence of a digenic transcript as well as the two individual messengers in both procyclic and bloodstream forms of the parasite. Southern blot analysis showed the relevant locus to be unique. ORF1 encoded a 22.7
kDa protein sharing over 50% identity with the eukaryotic PRCE (aka beta5) proteasome beta subunit. This protein contained a beta amino acid signature and residues involved in the catalytic activity. Further phylogenetic analysis indicated that this subunit as well as those from other kinetoplastids could be confidentially assigned to extant eukaryotic subfamilies such as beta1, beta2, and beta5. ORF2 encoded a 14.6
kDa putative zinc finger protein containing five repeats of a CCHC motif commonly present in retroviral nucleocapsid proteins as well as proteins involved in vertebrate embryogenesis.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Cysteine Endopeptidases - genetics</subject><subject>DNA, Complementary - chemistry</subject><subject>DNA, Complementary - genetics</subject><subject>Molecular Sequence Data</subject><subject>Multienzyme Complexes - genetics</subject><subject>Open Reading Frames</subject><subject>Orf1 gene</subject><subject>Orf2 gene</subject><subject>Phylogenetic analysis</subject><subject>Phylogeny</subject><subject>Polycistronic transcription</subject><subject>Proteasome Endopeptidase Complex</subject><subject>proteasomes</subject><subject>Protein Subunits</subject><subject>RNA, Messenger - genetics</subject><subject>Sequence Alignment</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Homology, Amino Acid</subject><subject>Transcription, Genetic</subject><subject>Trypanosoma brucei</subject><subject>Trypanosoma brucei brucei - genetics</subject><subject>Zinc Fingers - genetics</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9LJDEQxcOyi46zfoSVnBY9tFaSTqdzEpH9B8IedM8hSVeWLDPpMekW9NMb7UH2ZggUoX4vVbxHyBcG5wxYd3ELQvUNY0yfApwBCOga9oGsWK90U5_9R7J6Qw7JUSn_oB4p-QE5ZKBVKzuxIuGKTtmm4nPcTRSTH4eY_lJLd3mc0JZxi9ThZJsyuznFido01O5TTJ6GSmJeyJhovXf5cWfTWFWWujx7jPvymXwKdlPweF_X5M_3b3fXP5ub3z9-XV_dNF5oPjUtavRSDj5oG7QLymrmXAsBOQOrfAuCs6FjQmnFoeXBWw3CtaIPvbStE2vydfm3LnU_Y5nMNhaPm41NOM7FKM6F5Lp9F2RKdtBXek3kAvo8lpIxmF2OW5sfDQPzkoR5TcK82GwAzGsShlXdyX7A7LY4_KdarK_A5QJg9eMhYjbFxxoADjGjn8wwxndGPAO0D5ju</recordid><startdate>20000905</startdate><enddate>20000905</enddate><creator>Radwanska, Magdalena</creator><creator>Couvreur, Bernard</creator><creator>Dumont, Nathalie</creator><creator>Pays, Annette</creator><creator>Vanhamme, Luc</creator><creator>Pays, Etienne</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>20000905</creationdate><title>A transcript encoding a proteasome beta-subunit and a zinc finger protein in Trypanosoma brucei brucei</title><author>Radwanska, Magdalena ; Couvreur, Bernard ; Dumont, Nathalie ; Pays, Annette ; Vanhamme, Luc ; Pays, Etienne</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c392t-4e9ec55dcf9af9bf7a91bb40fe210a7c40321d6137972042fca903b438f85a4b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Cysteine Endopeptidases - genetics</topic><topic>DNA, Complementary - chemistry</topic><topic>DNA, Complementary - genetics</topic><topic>Molecular Sequence Data</topic><topic>Multienzyme Complexes - genetics</topic><topic>Open Reading Frames</topic><topic>Orf1 gene</topic><topic>Orf2 gene</topic><topic>Phylogenetic analysis</topic><topic>Phylogeny</topic><topic>Polycistronic transcription</topic><topic>Proteasome Endopeptidase Complex</topic><topic>proteasomes</topic><topic>Protein Subunits</topic><topic>RNA, Messenger - genetics</topic><topic>Sequence Alignment</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Homology, Amino Acid</topic><topic>Transcription, Genetic</topic><topic>Trypanosoma brucei</topic><topic>Trypanosoma brucei brucei - genetics</topic><topic>Zinc Fingers - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Radwanska, Magdalena</creatorcontrib><creatorcontrib>Couvreur, Bernard</creatorcontrib><creatorcontrib>Dumont, Nathalie</creatorcontrib><creatorcontrib>Pays, Annette</creatorcontrib><creatorcontrib>Vanhamme, Luc</creatorcontrib><creatorcontrib>Pays, Etienne</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Radwanska, Magdalena</au><au>Couvreur, Bernard</au><au>Dumont, Nathalie</au><au>Pays, Annette</au><au>Vanhamme, Luc</au><au>Pays, Etienne</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A transcript encoding a proteasome beta-subunit and a zinc finger protein in Trypanosoma brucei brucei</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>2000-09-05</date><risdate>2000</risdate><volume>255</volume><issue>1</issue><spage>43</spage><epage>50</epage><pages>43-50</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><abstract>During the screening of a
Trypanosoma brucei brucei (
T. b. brucei) cDNA library constructed from bloodstream form mRNA, we identified a 2.3
kb cDNA encoding a proteasome beta subunit (ORF1) and a putative zinc finger protein (ORF2). Northern blot analysis indicated the presence of a digenic transcript as well as the two individual messengers in both procyclic and bloodstream forms of the parasite. Southern blot analysis showed the relevant locus to be unique. ORF1 encoded a 22.7
kDa protein sharing over 50% identity with the eukaryotic PRCE (aka beta5) proteasome beta subunit. This protein contained a beta amino acid signature and residues involved in the catalytic activity. Further phylogenetic analysis indicated that this subunit as well as those from other kinetoplastids could be confidentially assigned to extant eukaryotic subfamilies such as beta1, beta2, and beta5. ORF2 encoded a 14.6
kDa putative zinc finger protein containing five repeats of a CCHC motif commonly present in retroviral nucleocapsid proteins as well as proteins involved in vertebrate embryogenesis.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>10974563</pmid><doi>10.1016/S0378-1119(00)00306-1</doi><tpages>8</tpages></addata></record> |
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| ispartof | Gene, 2000-09, Vol.255 (1), p.43-50 |
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| subjects | Amino Acid Sequence Animals Base Sequence Cysteine Endopeptidases - genetics DNA, Complementary - chemistry DNA, Complementary - genetics Molecular Sequence Data Multienzyme Complexes - genetics Open Reading Frames Orf1 gene Orf2 gene Phylogenetic analysis Phylogeny Polycistronic transcription Proteasome Endopeptidase Complex proteasomes Protein Subunits RNA, Messenger - genetics Sequence Alignment Sequence Analysis, DNA Sequence Homology, Amino Acid Transcription, Genetic Trypanosoma brucei Trypanosoma brucei brucei - genetics Zinc Fingers - genetics |
| title | A transcript encoding a proteasome beta-subunit and a zinc finger protein in Trypanosoma brucei brucei |
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