Rapid detection of Campylobacter fetus by polymerase chain reaction combined with non-radioactive hybridization using an oligonucleotide covalently bound to microwells
Campylobacter fetus is recognized as a human and animal pathogen. The isolation and differentiation of C. fetus in diagnostic laboratories is hindered by its relatively slow growth and lack of distinguishing biochemical characteristics. We cloned and sequenced a 1581-bp DNA fragment, IG02, isolated...
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| Veröffentlicht in: | Molecular and cellular probes 2000-08, Vol.14 (4), p.233-240 |
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| creator | Casadémont, I Bizet, C Chevrier, D Guesdon, J.-L |
| description | Campylobacter fetus is recognized as a human and animal pathogen. The isolation and differentiation of C. fetus in diagnostic laboratories is hindered by its relatively slow growth and lack of distinguishing biochemical characteristics. We cloned and sequenced a 1581-bp DNA fragment, IG02, isolated from a C. fetus genomic library. This fragment was used as a probe on DNAs extracted from C. fetus strains and otherCampylobacter species: IG02 hybridized only with DNAs from C. fetus strains. A PCR-based test was developed for the detection of C. fetus. A pair of oligonucleotide primers was designed to amplify a 141-bp fragment of IG02. The amplified product was analysed by a non-radioactive sandwich hybridization in microtiter plate using a capture oligonucleotide and a biotin-labelled oligonucleotide for the detection. The combination of PCR and non-radioactive microplate hybridization is a convenient method for the rapid detection of C. fetus. |
| doi_str_mv | 10.1006/mcpr.2000.0312 |
| format | Article |
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The isolation and differentiation of C. fetus in diagnostic laboratories is hindered by its relatively slow growth and lack of distinguishing biochemical characteristics. We cloned and sequenced a 1581-bp DNA fragment, IG02, isolated from a C. fetus genomic library. This fragment was used as a probe on DNAs extracted from C. fetus strains and otherCampylobacter species: IG02 hybridized only with DNAs from C. fetus strains. A PCR-based test was developed for the detection of C. fetus. A pair of oligonucleotide primers was designed to amplify a 141-bp fragment of IG02. The amplified product was analysed by a non-radioactive sandwich hybridization in microtiter plate using a capture oligonucleotide and a biotin-labelled oligonucleotide for the detection. The combination of PCR and non-radioactive microplate hybridization is a convenient method for the rapid detection of C. fetus.</description><identifier>ISSN: 0890-8508</identifier><identifier>EISSN: 1096-1194</identifier><identifier>DOI: 10.1006/mcpr.2000.0312</identifier><identifier>PMID: 10970727</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animals ; Campylobacter fetus - genetics ; Campylobacter fetus - isolation & purification ; Campylobacter fetus, diagnosis, PCR, sandwich hybridization ; DNA Primers - genetics ; Humans ; In Situ Hybridization - instrumentation ; In Situ Hybridization - methods ; Molecular Sequence Data ; Oligonucleotides - chemistry ; Oligonucleotides - genetics ; Polymerase Chain Reaction - methods ; Sensitivity and Specificity ; Sequence Analysis, DNA ; Species Specificity</subject><ispartof>Molecular and cellular probes, 2000-08, Vol.14 (4), p.233-240</ispartof><rights>2000 Academic Press</rights><rights>Copyright 2000 Academic Press.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c340t-2e22fb3b1ff7b8f8a985a91057beb4b31ed1ba425cbc6b7f6e3ffbef282df30d3</citedby><cites>FETCH-LOGICAL-c340t-2e22fb3b1ff7b8f8a985a91057beb4b31ed1ba425cbc6b7f6e3ffbef282df30d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/mcpr.2000.0312$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10970727$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Casadémont, I</creatorcontrib><creatorcontrib>Bizet, C</creatorcontrib><creatorcontrib>Chevrier, D</creatorcontrib><creatorcontrib>Guesdon, J.-L</creatorcontrib><title>Rapid detection of Campylobacter fetus by polymerase chain reaction combined with non-radioactive hybridization using an oligonucleotide covalently bound to microwells</title><title>Molecular and cellular probes</title><addtitle>Mol Cell Probes</addtitle><description>Campylobacter fetus is recognized as a human and animal pathogen. The isolation and differentiation of C. fetus in diagnostic laboratories is hindered by its relatively slow growth and lack of distinguishing biochemical characteristics. We cloned and sequenced a 1581-bp DNA fragment, IG02, isolated from a C. fetus genomic library. This fragment was used as a probe on DNAs extracted from C. fetus strains and otherCampylobacter species: IG02 hybridized only with DNAs from C. fetus strains. A PCR-based test was developed for the detection of C. fetus. A pair of oligonucleotide primers was designed to amplify a 141-bp fragment of IG02. The amplified product was analysed by a non-radioactive sandwich hybridization in microtiter plate using a capture oligonucleotide and a biotin-labelled oligonucleotide for the detection. The combination of PCR and non-radioactive microplate hybridization is a convenient method for the rapid detection of C. fetus.</description><subject>Animals</subject><subject>Campylobacter fetus - genetics</subject><subject>Campylobacter fetus - isolation & purification</subject><subject>Campylobacter fetus, diagnosis, PCR, sandwich hybridization</subject><subject>DNA Primers - genetics</subject><subject>Humans</subject><subject>In Situ Hybridization - instrumentation</subject><subject>In Situ Hybridization - methods</subject><subject>Molecular Sequence Data</subject><subject>Oligonucleotides - chemistry</subject><subject>Oligonucleotides - genetics</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Sensitivity and Specificity</subject><subject>Sequence Analysis, DNA</subject><subject>Species Specificity</subject><issn>0890-8508</issn><issn>1096-1194</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kT2P1DAQhi0E4vYOWkrkii6L7WQ3TolWHJx0EhKC2vLH-NbIsYPt7Cn8If4mDrmChmqKeeadGT0IvaFkTwk5vh_1lPaMELInLWXP0I6S4dhQOnTP0Y7wgTT8QPgVus75R6WGjvCX6KpCPelZv0O_v8rJGWyggC4uBhwtPslxWnxUUhdI2EKZM1YLnqJfRkgyA9Zn6QJOILcZHUflAhj86MoZhxiaJI2La_cC-Lyo5Iz7Jf-yc3bhAcu6yLuHGGbtIRZnama8SA-h-AWrOAeDS8Sj0yk-gvf5FXphpc_w-qneoO-3H7-dPjf3Xz7dnT7cN7rtSGkYMGZVq6i1veKWy4Ef5EDJoVegOtVSMFTJjh200kfV2yO01iqwjDNjW2LaG_Ruy51S_DlDLmJ0WdcLZIA4Z9Ez1naMdRXcb2C9MOcEVkzJjTItghKxqhGrGrGqEauaOvD2KXlWI5h_8M1FBfgGQP3v4iCJrB0EDcalKkeY6P6X_Qc_DKRQ</recordid><startdate>20000801</startdate><enddate>20000801</enddate><creator>Casadémont, I</creator><creator>Bizet, C</creator><creator>Chevrier, D</creator><creator>Guesdon, J.-L</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20000801</creationdate><title>Rapid detection of Campylobacter fetus by polymerase chain reaction combined with non-radioactive hybridization using an oligonucleotide covalently bound to microwells</title><author>Casadémont, I ; Bizet, C ; Chevrier, D ; Guesdon, J.-L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c340t-2e22fb3b1ff7b8f8a985a91057beb4b31ed1ba425cbc6b7f6e3ffbef282df30d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>Campylobacter fetus - genetics</topic><topic>Campylobacter fetus - isolation & purification</topic><topic>Campylobacter fetus, diagnosis, PCR, sandwich hybridization</topic><topic>DNA Primers - genetics</topic><topic>Humans</topic><topic>In Situ Hybridization - instrumentation</topic><topic>In Situ Hybridization - methods</topic><topic>Molecular Sequence Data</topic><topic>Oligonucleotides - chemistry</topic><topic>Oligonucleotides - genetics</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Sensitivity and Specificity</topic><topic>Sequence Analysis, DNA</topic><topic>Species Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Casadémont, I</creatorcontrib><creatorcontrib>Bizet, C</creatorcontrib><creatorcontrib>Chevrier, D</creatorcontrib><creatorcontrib>Guesdon, J.-L</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular and cellular probes</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Casadémont, I</au><au>Bizet, C</au><au>Chevrier, D</au><au>Guesdon, J.-L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid detection of Campylobacter fetus by polymerase chain reaction combined with non-radioactive hybridization using an oligonucleotide covalently bound to microwells</atitle><jtitle>Molecular and cellular probes</jtitle><addtitle>Mol Cell Probes</addtitle><date>2000-08-01</date><risdate>2000</risdate><volume>14</volume><issue>4</issue><spage>233</spage><epage>240</epage><pages>233-240</pages><issn>0890-8508</issn><eissn>1096-1194</eissn><abstract>Campylobacter fetus is recognized as a human and animal pathogen. The isolation and differentiation of C. fetus in diagnostic laboratories is hindered by its relatively slow growth and lack of distinguishing biochemical characteristics. We cloned and sequenced a 1581-bp DNA fragment, IG02, isolated from a C. fetus genomic library. This fragment was used as a probe on DNAs extracted from C. fetus strains and otherCampylobacter species: IG02 hybridized only with DNAs from C. fetus strains. A PCR-based test was developed for the detection of C. fetus. A pair of oligonucleotide primers was designed to amplify a 141-bp fragment of IG02. The amplified product was analysed by a non-radioactive sandwich hybridization in microtiter plate using a capture oligonucleotide and a biotin-labelled oligonucleotide for the detection. The combination of PCR and non-radioactive microplate hybridization is a convenient method for the rapid detection of C. fetus.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>10970727</pmid><doi>10.1006/mcpr.2000.0312</doi><tpages>8</tpages></addata></record> |
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| ispartof | Molecular and cellular probes, 2000-08, Vol.14 (4), p.233-240 |
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| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Campylobacter fetus - genetics Campylobacter fetus - isolation & purification Campylobacter fetus, diagnosis, PCR, sandwich hybridization DNA Primers - genetics Humans In Situ Hybridization - instrumentation In Situ Hybridization - methods Molecular Sequence Data Oligonucleotides - chemistry Oligonucleotides - genetics Polymerase Chain Reaction - methods Sensitivity and Specificity Sequence Analysis, DNA Species Specificity |
| title | Rapid detection of Campylobacter fetus by polymerase chain reaction combined with non-radioactive hybridization using an oligonucleotide covalently bound to microwells |
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