Ion intensity analysis of post-source decay fragmentation in curved-field reflectron matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of carbohydrates: For structural characterization of glycosylation in proteome analysis
The various kinds of oligosaccharides were analyzed by using post‐source decay (PSD) fragmentation method of matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOF MS). Since the curved field reflectron MALDI‐MS can record all fragment ions in a single measurement, w...
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| Veröffentlicht in: | Proteomics (Weinheim) 2001-02, Vol.1 (2), p.329-339 |
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| creator | Yamagaki, Tohru Nakanishi, Hiroshi |
| description | The various kinds of oligosaccharides were analyzed by using post‐source decay (PSD) fragmentation method of matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOF MS). Since the curved field reflectron MALDI‐MS can record all fragment ions in a single measurement, we can discuss the fragment ion intensity in PSD mass spectrum more accurately. The intensities of the PSD fragment ions indicate the fine structure of the saccharide chains. The type of glycosyl linkages could be determined by the ion intensity analysis, and the stereo isomers of monosaccharides were distinguished by the MALDI‐PSD fragment ion analysis. The linkage isomers and structural isomers were also distinguished by this method. The ion intensity analysis of curved‐field reflectron MALDI‐MS could be a powerful tool for glycosylation analysis. |
| doi_str_mv | 10.1002/1615-9861(200102)1:2<329::AID-PROT329>3.0.CO;2-K |
| format | Article |
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The ion intensity analysis of curved‐field reflectron MALDI‐MS could be a powerful tool for glycosylation analysis.</description><subject>Carbohydrate Conformation</subject><subject>Carbohydrate Sequence</subject><subject>Carbohydrates - analysis</subject><subject>Fragment</subject><subject>Glycoproteins - chemistry</subject><subject>Glycosylation</subject><subject>Ionization mass spectrometry</subject><subject>Lewis Blood-Group System - chemistry</subject><subject>Matrix-assisted laser desorption</subject><subject>Molecular Sequence Data</subject><subject>Oligosaccharide</subject><subject>Oligosaccharides - chemistry</subject><subject>Post-source decay</subject><subject>Protein Processing, Post-Translational</subject><subject>Proteome - chemistry</subject><subject>proteomics</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods</subject><issn>1615-9853</issn><issn>1615-9861</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkl9v0zAUxSMEYmPwFZCfEDyks502cQpCmgorVQutoGiPlmPfdIYkLrYDC9-cN5ylKy9I8BD53zm_e-2cKGIEjwjG9JykZBLnLCXPKcYE0xdkSl8lNJ9OLxZv4s3H9TYsXicjPJqtX9J4eS86PVruH-eT5CR65NyXgMhYnj2MTghJGWZZfhr9WpgG6cZD47TvkGhE1TntkCnR3jgfO9NaCUiBFB0qrdjV0Hjh9a0LydZ-BxWXGiqFLJQVSG_DUS281TexcAHlQaFKOLAB4ozd997z8OmfA8brGmJTxmWld9c-WJ1Dbn8LqsHbrm9FCluY605Z4cFN0aWxyHnbSt9aUSF5LayQHuwdMjh2VSeN66pjq3trPATi8YqPowelqBw8OYxn0efLt9vZu3i1ni9mF6tYjnOax1QylsmCpgxwOVbjXEiqxoLRgqlUJYmapLnIMiHyIkhFBiUtJFBIJ6oAEt7-LHo2cEMH31pwntfaSagq0YBpHc8oTRKW4H8KCcMZIYQG4WYQSmucC8_O91bXwnacYN7nhvc_nvch4ENueJjwEBXOQ274ITc84ZjP1uFkGZBPD7Xbogb1B3gIShBsB8EPXUH3_wX_Xu9uK2DjAdvn5OaIFfYrT7Mkm_CrD3O-ypfvN_mnKz5PfgPkl_Y3</recordid><startdate>200102</startdate><enddate>200102</enddate><creator>Yamagaki, Tohru</creator><creator>Nakanishi, Hiroshi</creator><general>WILEY-VCH Verlag GmbH</general><general>WILEY‐VCH Verlag GmbH</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>200102</creationdate><title>Ion intensity analysis of post-source decay fragmentation in curved-field reflectron matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of carbohydrates: For structural characterization of glycosylation in proteome analysis</title><author>Yamagaki, Tohru ; 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Since the curved field reflectron MALDI‐MS can record all fragment ions in a single measurement, we can discuss the fragment ion intensity in PSD mass spectrum more accurately. The intensities of the PSD fragment ions indicate the fine structure of the saccharide chains. The type of glycosyl linkages could be determined by the ion intensity analysis, and the stereo isomers of monosaccharides were distinguished by the MALDI‐PSD fragment ion analysis. The linkage isomers and structural isomers were also distinguished by this method. The ion intensity analysis of curved‐field reflectron MALDI‐MS could be a powerful tool for glycosylation analysis.</abstract><cop>Weinheim</cop><pub>WILEY-VCH Verlag GmbH</pub><pmid>11680879</pmid><doi>10.1002/1615-9861(200102)1:2<329::AID-PROT329>3.0.CO;2-K</doi><tpages>11</tpages></addata></record> |
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| subjects | Carbohydrate Conformation Carbohydrate Sequence Carbohydrates - analysis Fragment Glycoproteins - chemistry Glycosylation Ionization mass spectrometry Lewis Blood-Group System - chemistry Matrix-assisted laser desorption Molecular Sequence Data Oligosaccharide Oligosaccharides - chemistry Post-source decay Protein Processing, Post-Translational Proteome - chemistry proteomics Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods |
| title | Ion intensity analysis of post-source decay fragmentation in curved-field reflectron matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of carbohydrates: For structural characterization of glycosylation in proteome analysis |
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